CD117 antibody | 104D2
Mouse anti Human CD117 antibody, clone 104D2 (MCA1841) used for the evaluation of c-kit (CD117) expression by flow cytometry.
Image caption:
Purity of Mast cells. A. Flow cytometry analysis of intracellular chymase expression. Human cord blood-derived mast cells labeled for intracellular mast-cell chymase expression (solid line), isotype control (Black area). Results shown are representative of four separate experiments. B. Toluidine blue staining of mast cells. Fluorescence microscopy of human cord blood-derived mast cells stained with Toluidine blue. Results shown are representative of at least three separate experiments. C. Flow cytometry analysis of cell surface expression of c-kit and FcεRI. Human cord blood-derived mast cells labelled for cell-surface expression of c-kit and FceRI (left panel), and isotype controls (right panel). Results shown are representative of four separate experiments.
From: Jayapal M, Tay HK, Reghunathan R, Zhi L, Chow KK, Rauff M, Melendez AJ.
Genome-wide gene expression profiling of human mast cells stimulated by IgE or FcepsilonRI-aggregation reveals a complex network of genes involved in inflammatory responses.
BMC Genomics. 2006 Aug 16;7:210.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD117 antibody, clone 104D2 recognizes the human CD117 cell surface antigen, a 145 kDa glycoprotein also known as c-kit. CD117 is expressed by some haematopoietic stem cells (approx. 70% of CD34+ve cells in normal bone marrow are CD117+ve), some acute myeloid leukaemias and by mast cells.
Functionally CD117 is a receptor for stem cell factor, and has receptor tyrosine kinase activity.
Mouse anti Human CD117 antibody, clone 104D2 is non-neutralizing.
Functionally CD117 is a receptor for stem cell factor, and has receptor tyrosine kinase activity.
Mouse anti Human CD117 antibody, clone 104D2 is non-neutralizing.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Cynomolgus monkey Bovine - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 0.2% Bovine Serum Albumin - Immunogen
- MOLM-1 megakaryocyte cell line
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
- Guarantee
- 6 months from date of despatch
More Information
- UniProt
- P10721
- Entrez Gene
- KIT
- GO Terms
- GO:0005515 protein binding
- GO:0016021 integral to membrane
- GO:0004716 receptor signaling protein tyrosine kinase activity
- GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
- GO:0005524 ATP binding
- GO:0005615 extracellular space
- GO:0008584 male gonad development
- Regulatory
- For research purposes only
Applications of CD117 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/2 | 1/20 | |
| Flow Cytometry | Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 5ul of the suggested working dilution to label 106 cells in 100ul
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
|
| Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
References for CD117 antibody
-
Broudy, V.C. et al. (1998) Analysis of c-kit receptor dimerization by fluorescence resonance energy transfer.
Blood. 91 (3): 898-906. -
Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.
Exp Anim. 49 (2): 97-110. -
Jayapal, M. et al. (2006) Genome-wide gene expression profiling of human mast cells stimulated by IgE or FcepsilonRI-aggregation reveals a complex network of genes involved in inflammatory responses.
BMC Genomics. 7: 210. -
Randall, V.A. et al. (2008) Stem cell factor/c-Kit signalling in normal and androgenetic alopecia hair follicles.
J Endocrinol. 197: 11-23. -
Venkata, N.S.G. et al. (2018) Isolation and characterization of mesenchymal stem cells from human fetus heart.
PLoS One. 13 (2): e0192244.
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