CD117 antibody | 104D2

Mouse anti Human CD117

Product Type
Monoclonal Antibody
Clone
104D2
Isotype
IgG1
Specificity
CD117

Product Code Applications Pack Size List Price Your Price Qty
MCA1841
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human CD117 antibody, clone 104D2 recognizes the human CD117 cell surface antigen, a 145 kDa glycoprotein also known as c-kit. CD117 is expressed by some haematopoietic stem cells (approx. 70% of CD34+ve cells in normal bone marrow are CD117+ve), some acute myeloid leukaemias and by mast cells.

Functionally CD117 is a receptor for stem cell factor, and has receptor tyrosine kinase activity.

Mouse anti Human CD117 antibody, clone 104D2 is non-neutralizing.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Cynomolgus monkey
Bovine
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
MOLM-1 megakaryocyte cell line
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/2 1/20
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG (Fc):HRP
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)

Description Product Code Applications Pack Size List Price Your Price Quantity
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Description Mouse IgG1 Negative Control

References for CD117 antibody

  1. Broudy, V.C. et al. (1998) Analysis of c-kit receptor dimerization by fluorescence resonance energy transfer.
    Blood. 91 (3): 898-906.
  2. Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.
    Exp Anim. 49 (2): 97-110.
  3. Jayapal, M. et al. (2006) Genome-wide gene expression profiling of human mast cells stimulated by IgE or FcepsilonRI-aggregation reveals a complex network of genes involved in inflammatory responses.
    BMC Genomics. 7: 210.
  4. Randall, V.A. et al. (2008) Stem cell factor/c-Kit signalling in normal and androgenetic alopecia hair follicles.
    J Endocrinol. 197: 11-23.
  5. Venkata, N.S.G. et al. (2018) Isolation and characterization of mesenchymal stem cells from human fetus heart.
    PLoS One. 13 (2): e0192244.

Synonyms
C-KIT
SCF-R
RRID
AB_322902
UniProt
P10721
Entrez Gene
KIT
GO Terms
GO:0005515 protein binding
GO:0016021 integral to membrane
GO:0004716 receptor signaling protein tyrosine kinase activity
GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
GO:0005524 ATP binding
GO:0005615 extracellular space
GO:0008584 male gonad development
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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