CD112 antibody | R2-525

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Mouse anti Human CD112

Product Type
Monoclonal Antibody
Clone
R2-525
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1937T C F IP 25 µg
MCA1937 C F IP 0.2 mg
Mouse anti Human CD112 antibody, clone R2-525 recognizes CD112, also known as Poliovirus Receptor Related 2, PRR2 or Nectin-2, a surface molecule originally reported to be homologous to CD155, the poliovirus receptor (Lopez et al. 1998). PRR2 expression is restricted to the myelo-monocytic and megakaryocytic lineages. PRR2 expression has also been detected on haematopoeitic progenitors expressing CD34.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Approx. Protein Concentrations
Isotype concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q92692 Related reagents
Entrez Gene
PVRL2 Related reagents
GO Terms
GO:0002860 positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target
GO:0002891 positive regulation of immunoglobulin mediated immune response
GO:0016021 integral to membrane
GO:0050839 cell adhesion molecule binding
GO:0005915 zonula adherens
GO:0007156 homophilic cell adhesion
GO:0007286 spermatid development
GO:0009986 cell surface
GO:0015026 coreceptor activity
GO:0019064 viral envelope fusion with host membrane
GO:0033005 positive regulation of mast cell activation
GO:0034329 cell junction assembly
GO:0034332 adherens junction organization
GO:0042271 susceptibility to natural killer cell mediated cytotoxicity
GO:0042803 protein homodimerization activity
GO:0046814 virion attachment, binding of host cell surface coreceptor
GO:0051856 adhesion to symbiont
GO:0060370 susceptibility to T cell mediated cytotoxicity
Regulatory
For research purposes only

Applications of CD112 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunohistology - Frozen
Immunoprecipitation
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA928 100 Tests F

Product Specific References

References for CD112 antibody

  1. Lopez, M. et al. (1998) The human poliovirus receptor related 2 protein is a new hematopoietic/endothelial homophilic adhesion molecule.
    Blood. 92 (12): 4602-11.
  2. Goyert, S.M. (1998) White cell differentiation antigens, Myeloid Antigens: section report.
    Leucocyte typing VI. Kishimoto, T. et al. eds (Garland Publishing Inc. New York) p. 956-957
  3. Cocchi F, et al. (2000) Cell-to-cell spread of wild-type herpes simplex virus type 1, but not of syncytial strains, is mediated by the immunoglobulin-like receptors that mediate virion entry, nectin1 (PRR1/HveC/HIgR) and nectin2 (PRR2/HveB).
    J Virol. 74 (8): 3909-17.
  4. Verheyden, S. et al. (2009) Role of the inhibitory KIR ligand HLA-Bw4 and HLA-C expression levels in the recognition of leukemic cells by Natural Killer cells.
    Cancer Immunol Immunother. 58 (6): 855-65.
  5. Fionda, C. et al. (2009) Heat shock protein-90 inhibitors increase MHC class I-related chain A and B ligand expression on multiple myeloma cells and their ability to trigger NK cell degranulation.
    J Immunol. 183 (7): 4385-94.