CD103 antibody | 2G5.1
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Mouse anti Human CD103
- Product Type
- Monoclonal Antibody
- Clone
- 2G5.1
- Isotype
- IgG2a
- Specificity
- CD103
Antibody Quality and Performance Guarantee
| Mouse anti Human CD103 antibody, clone 2G5.1 recognizes the human CD103 cell surface antigen, also known as the alphaE integrin, HML-1 antigen, Mucosal lymphocyte 1 antigen or Integrin alpha-E. CD103 is a 1161 amino acid, ~130 kDa single pass type 1 transmembrane glycoprotein. Mouse anti Human CD103 antibody, clone 2G5.1 reacts with various subsets of human intra-epithelial lymphocytes:- 40% of lamina propria T-cells; 30% of mesenteric lymphoblasts and some lymphocytes from other mucosa. It recognises less than 0.1% bone marrow cells and less than 0.7% PBL. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Cynomolgus monkey Rhesus Monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Immunogen
- Human intestinal lymphocytes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the NS1 mouse myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/100 |
| Immunofluorescence | |
||
| Immunohistology - Frozen 1 | |
1/50 | 1/100 |
| Immunohistology - Paraffin | |||
| Immunoprecipitation | |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 5 x 105 cells in 100ul.
- Histology Positive Control Tissue
- Intestine
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG2a Negative Control | ||||||
References for CD103 antibody
-
Cerf-Bensussan, N. et al. (1986) A monoclonal antibody specific for rat intestinal lymphocytes.
J Immunol. 136 (1): 76-82. -
Cerf-Bensussan, N. et al. (1987) A monoclonal antibody (HML-1) defining a novel membrane molecule present on human intestinal lymphocytes.
Eur J Immunol. 17 (9): 1279-85. -
Kutleša, S. et al. (2002) E-cadherin-mediated interactions of thymic epithelial cells with CD103+ thymocytes lead to enhanced thymocyte cell proliferation.
J Cell Sci. 115 (Pt 23): 4505-15. -
Presicce P et al. (2012) Myeloid dendritic cells isolated from tissues of SIV-infected Rhesus macaques promote the induction of regulatory T cells.
AIDS. 26 (3): 263-73. -
Swan, Z.D. et al. (2016) Macrophage accumulation in gut mucosa differentiates AIDS from chronic SIV infection in rhesus macaques.
Eur J Immunol. 46 (2): 446-54. -
Nugent, J.L. et al. (2021) A nonhuman primate model of vertical sleeve gastrectomy facilitates mechanistic and translational research in human obesity.
iScience. 24 (12): 103421.
- RRID
- AB_321984
- UniProt
- P38570
- Entrez Gene
- ITGAE
- GO Terms
- GO:0007155 cell adhesion
- GO:0004872 receptor activity
- GO:0008305 integrin complex
- GO:0007229 integrin-mediated signaling pathway
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