CD103 antibody | 2G5.1
Mouse anti Human CD103
- Product Type
- Monoclonal Antibody
|Mouse anti Human CD103 antibody, clone 2G5.1 recognizes the human CD103 cell surface antigen, also known as the alphaE integrin, HML-1 antigen, Mucosal lymphocyte 1 antigen or Integrin alpha-E. CD103 is a 1161 amino acid, ~130 kDa single pass type 1 transmembrane glycoprotein.
Mouse anti Human CD103 antibody, clone 2G5.1 reacts with various subsets of human intra-epithelial lymphocytes:- 40% of lamina propria T-cells; 30% of mesenteric lymphoblasts and some lymphocytes from other mucosa. It recognises less than 0.1% bone marrow cells and less than 0.7% PBL.
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Cynomolgus monkey Rhesus Monkey
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
- Human intestinal lymphocytes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the NS1 mouse myeloma cell line
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen 1||1/50||1/100|
|Immunohistology - Paraffin|
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 5 x 105 cells in 100ul.
- Histology Positive Control Tissue
References for CD103 antibody
Cerf-Bensussan, N. et al. (1986) A monoclonal antibody specific for rat intestinal lymphocytes.
J Immunol. 136 (1): 76-82.
Cerf-Bensussan, N. et al. (1987) A monoclonal antibody (HML-1) defining a novel membrane molecule present on human intestinal lymphocytes.
Eur J Immunol. 17 (9): 1279-85.
Kutleša, S. et al. (2002) E-cadherin-mediated interactions of thymic epithelial cells with CD103+ thymocytes lead to enhanced thymocyte cell proliferation.
J Cell Sci. 115 (Pt 23): 4505-15.
Presicce P et al. (2012) Myeloid dendritic cells isolated from tissues of SIV-infected Rhesus macaques promote the induction of regulatory T cells.
AIDS. 26 (3): 263-73.
Swan, Z.D. et al. (2016) Macrophage accumulation in gut mucosa differentiates AIDS from chronic SIV infection in rhesus macaques.
Eur J Immunol. 46 (2): 446-54.
Nugent, J.L. et al. (2021) A nonhuman primate model of vertical sleeve gastrectomy facilitates mechanistic and translational research in human obesity.
iScience. 24 (12): 103421.
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