CD103 antibody | 2G5.1

Mouse anti Human CD103

Product Type
Monoclonal Antibody
Clone
2G5.1
Isotype
IgG2a
Specificity
CD103

Product Code Applications Pack Size List Price Your Price Qty
MCA708
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * F IF IP 0.2 mg loader
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loader
MCA708T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * F IF IP 25 µg loader
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loader
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Mouse anti Human CD103 antibody, clone 2G5.1 recognizes the human CD103 cell surface antigen, also known as the alphaE integrin, HML-1 antigen, Mucosal lymphocyte 1 antigen or Integrin alpha-E. CD103 is a 1161 amino acid, ~130 kDa single pass type 1 transmembrane glycoprotein.

Mouse anti Human CD103 antibody, clone 2G5.1 reacts with various subsets of human intra-epithelial lymphocytes:- 40% of lamina propria T-cells; 30% of mesenteric lymphoblasts and some lymphocytes from other mucosa. It recognises less than 0.1% bone marrow cells and less than 0.7% PBL.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Cynomolgus monkey
Rhesus Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Human intestinal lymphocytes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunofluorescence
Immunohistology - Frozen 1 1/50 1/100
Immunohistology - Paraffin
Immunoprecipitation
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 5 x 105 cells in 100ul.
Histology Positive Control Tissue
Intestine

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Description Human anti Mouse IgG2a:HRP
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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG (Fc):HRP
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG2a Negative Control MCA929 F 100 Tests loader
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Description Mouse IgG2a Negative Control

References for CD103 antibody

  1. Cerf-Bensussan, N. et al. (1987) A monoclonal antibody (HML-1) defining a novel membrane molecule present on human intestinal lymphocytes.
    Eur J Immunol. 17 (9): 1279-85.
  2. Cerf-Bensussan, N. et al. (1986) A monoclonal antibody specific for rat intestinal lymphocytes.
    J Immunol. 136 (1): 76-82.
  3. Sopper, S. et al. (1997) Lymphocyte subsets and expression of differentiation markers in blood and lymphoid organs of rhesus monkeys.
    Cytometry. 29 (4): 351-62.
  4. Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of Cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.
    Exp. Anim. 49: 97-110.
  5. Swan, Z.D. et al. (2015) Macrophage accumulation in gut mucosa differentiates AIDS from chronic SIV infection in rhesus macaques.
    Eur J Immunol. Nov 9. [Epub ahead of print]
  6. Presicce P et al. (2012) Myeloid dendritic cells isolated from tissues of SIV-infected Rhesus macaques promote the induction of regulatory T cells.
    AIDS. 26 (3): 263-73.
  7. Kutleša, S. et al. (2002) E-cadherin-mediated interactions of thymic epithelial cells with CD103+ thymocytes lead to enhanced thymocyte cell proliferation.
    J Cell Sci. 115 (Pt 23): 4505-15.
  8. Nugent, J.L. et al. (2021) A nonhuman primate model of vertical sleeve gastrectomy facilitates mechanistic and translational research in human obesity.
    iScience. 24 (12): 103421.
  9. View The Latest Product References

RRID
AB_321984
UniProt
P38570
Entrez Gene
ITGAE
GO Terms
GO:0007155 cell adhesion
GO:0004872 receptor activity
GO:0008305 integrin complex
GO:0007229 integrin-mediated signaling pathway
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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