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CD10 antibody | SN5c

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Anti Human CD10 Antibody, clone SN5c thumbnail image 1 Anti Human CD10 Antibody, clone SN5c thumbnail image 2 Anti Human CD10 Antibody, clone SN5c thumbnail image 3 Anti Human CD10 Antibody, clone SN5c thumbnail image 4 Anti Human CD10 Antibody, clone SN5c thumbnail image 5 Anti Human CD10 Antibody, clone SN5c thumbnail image 6 Anti Human CD10 Antibody, clone SN5c thumbnail image 7 Anti Human CD10 Antibody, clone SN5c thumbnail image 8 Anti Human CD10 Antibody, clone SN5c thumbnail image 9 Anti Human CD10 Antibody, clone SN5c thumbnail image 10 Anti Human CD10 Antibody, clone SN5c thumbnail image 11 Anti Human CD10 Antibody, clone SN5c thumbnail image 12 Anti Human CD10 Antibody, clone SN5c thumbnail image 13 Anti Human CD10 Antibody, clone SN5c thumbnail image 14 Anti Human CD10 Antibody, clone SN5c thumbnail image 15 Anti Human CD10 Antibody, clone SN5c thumbnail image 16 Anti Human CD10 Antibody, clone SN5c thumbnail image 17 Anti Human CD10 Antibody, clone SN5c thumbnail image 18 Anti Human CD10 Antibody, clone SN5c thumbnail image 19 Anti Human CD10 Antibody, clone SN5c thumbnail image 20 Anti Human CD10 Antibody, clone SN5c thumbnail image 21 Anti Human CD10 Antibody, clone SN5c thumbnail image 22 Anti Human CD10 Antibody, clone SN5c thumbnail image 23
Anti Human CD10 Antibody, clone SN5c gallery image 1
Figure A. FITC conjugated Mouse anti Human CD11b (MCA551F) and Amethyst Orange conjugated Mouse IgG1 isotype control (MCA928AMO). Figure B. FITC conjugated Mouse anti Human CD3 (MCA463F) and Amethyst Orange conjugated Mouse anti Human CD10 (MCA1556AMO). All experiments performed on human blood gated on live single granulocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
Anti Human CD10 Antibody, clone SN5c gallery image 2
Figure A. FITC conjugated mouse anti human CD19 (MCA1940F) and Alexa647® conjugated Mouse IgG1 isotype control (MCA928A647). Figure B. FITC conjugated mouse anti human CD19 (MCA1940F) and Alexa647® conjugated mouse anti human CD10 (MCA1556A647). All experiments performed on red cell lysed Human peripheral blood gated on leukocytes in the presence of Human SeroBlock (BUF070A).
Anti Human CD10 Antibody, clone SN5c gallery image 3
Figure A. FITC conjugated Mouse anti Human CD11b (MCA551F). Figure B. FITC conjugated Mouse anti Human CD11b (MCA551F) and StarBright Violet 670 conjugated Mouse anti Human CD10 (MCA1556SBV670). All experiments performed on red cell lysed human blood gated on live single cell granulocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD10 Antibody, clone SN5c gallery image 4
Figure A. FITC conjugated Mouse anti Human CD11b (MCA551F). Figure B. FITC conjugated Mouse anti Human CD11b (MCA551F) and StarBright Violet 440 conjugated Mouse anti Human CD10 (MCA1556SBV440). All experiments performed on red cell lysed human blood gated on live single granulocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD10 Antibody, clone SN5c gallery image 5
Figure A. PE conjugated Mouse anti Human CD11b (MCA551PE). Figure B. PE conjugated Mouse anti Human CD11b (MCA551PE) and StarBright Violet 610 conjugated Mouse anti Human CD10 (MCA1556SBV610). All experiments performed on red blood lysed human blood gated on live single cell granulocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD10 Antibody, clone SN5c gallery image 6
Figure A. PE conjugated Mouse anti Human CD11b (MCA551PE). Figure B. PE conjugated Mouse anti Human CD11b (MCA551PE) and StarBright Violet 570 conjugated Mouse anti Human CD10 (MCA1556SBV570). All experiments performed on red cell lysed human blood gated on live single cell granulocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD10 Antibody, clone SN5c gallery image 7
Figure A. FITC conjugated Mouse anti Human CD11b (MCA551F). Figure B. FITC conjugated Mouse anti Human CD11b (MCA551F) and StarBright Violet 710 conjugated Mouse anti Human CD10 (MCA1556SBV710). All experiments performed on red cell lysed human blood gated on live single cell granulocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD10 Antibody, clone SN5c gallery image 8
Figure A. PE conjugated Mouse anti Human CD11b (MCA551PE). Figure B. PE conjugated Mouse anti Human CD11b (MCA551PE) and StarBright Violet 790 conjugated Mouse anti Human CD10 (MCA1556SBV790). All experiments performed on red cell lysed human blood gated on live single cell granulocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD10 Antibody, clone SN5c gallery image 9
Figure A. FITC conjugated Mouse anti Human CD11b (MCA551F) . Figure B. FITC conjugated Mouse anti Human CD11b (MCA551F) and StarBright Violet 475 conjugated Mouse anti Human CD10 (MCA1556SBV475). All experiments performed on red cell lysed human blood gated on live single cell granulocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD10 Antibody, clone SN5c gallery image 10
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD11b (MCA74A647). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD11b (MCA74A647) and StarBright Violet 760 conjugated Mouse anti Human CD10 (MCA1556SBV760). All experiments performed on red cell lysed human blood gated on live single granulocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD10 Antibody, clone SN5c gallery image 11
Figure A. PE conjugated Mouse anti Human CD11b (MCA551PE). Figure B. PE conjugated Mouse anti Human CD11b (MCA551PE) and StarBright UltraViolet 665 conjugated Mouse anti Human CD10 (MCA1556SBUV665). All experiments performed on red cell lysed human blood gated on live granulocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD10 Antibody, clone SN5c gallery image 12
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD11b (MCA551A647). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD11b (MCA551A647) and StarBright UltraViolet 795 conjugated Mouse anti Human CD10 (MCA1556SBUV795). All experiments performed on red cell lysed human blood gated on live single cell Granulocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD10 Antibody, clone SN5c gallery image 13
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD11b (MCA551A647). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD11b (MCA551A647) and StarBright Blue 580 conjugated Mouse anti Human CD10 (MCA1556SBB580). All experiments performed on red cell lysed human blood gated on live single cell granulocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD10 Antibody, clone SN5c gallery image 14
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD11b (MCA551A647). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD11b (MCA551A647) and StarBright Blue 615 conjugated Mouse anti Human CD10 (MCA1556SBB615). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD10 Antibody, clone SN5c gallery image 15
Figure A. RPE conjugated Mouse anti Human CD11b (MCA551PE). Figure B. RPE conjugated Mouse anti Human CD11b (MCA551PE) and StarBright Blue 675 conjugated Mouse anti Human CD10 (MCA1556SBB675). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD10 Antibody, clone SN5c gallery image 16
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD11b (MCA551A647). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD11b (MCA551A647) and StarBright Blue 765 conjugated Mouse anti Human CD10 (MCA1556SBB765). All experiments performed on red cell lysed human blood gated on live single cell granulocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD10 Antibody, clone SN5c gallery image 17
Figure A. RPE conjugated Mouse anti Human CD11b (MCA551PE). Figure B. RPE conjugated Mouse anti Human CD11b (MCA551PE) and StarBright Blue 810 conjugated Mouse anti Human CD10 (MCA1556SBB810). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD10 Antibody, clone SN5c gallery image 18
Figure A. FITC conjugated Mouse anti Human CD11b (MCA551F). Figure B. FITC conjugated Mouse anti Human CD11b (MCA551F) and StarBright Yellow 575 conjugated Mouse anti Human CD10 (MCA1556SBY575). All experiments performed on red cell lysed Human blood gated on live single granulocytes, in the presence of 10% Human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD10 Antibody, clone SN5c gallery image 19
Figure A. Pacific Blue conjugated Mouse anti Human CD11b (MCA551PB). Figure B. Pacific Blue conjugated Mouse anti Human CD11b (MCA551PB) and StarBright Yellow 720 conjugated Mouse anti Human CD10 (MCA1556SBY720). All experiments performed on human blood gated on live single granulocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD10 Antibody, clone SN5c gallery image 20
Figure A. Alexa fluor 700 conjugated Mouse anti Human CD11b (MCA551A700). Figure B. Alexa fluor 700 conjugated Mouse anti Human CD11b (MCA551A700) and StarBright Yellow 605 conjugated Mouse anti Human CD10 (MCA1556SBY605). All experiments performed on human blood gated on live single cell granulocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD10 Antibody, clone SN5c gallery image 21
Figure A. FITC conjugated Mouse anti Human CD11b (MCA551F). Figure B. FITC conjugated Mouse anti Human CD11b (MCA551F) and StarBright Yellow 665 conjugated Mouse anti Human CD10 (MCA1556SBY665). All experiments performed on red cell lysed human blood gated on live single cell granulocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD10 Antibody, clone SN5c gallery image 22
Figure A. RPE conjugated Mouse anti Human CD11b (MCA551PE). Figure B. RPE conjugated Mouse anti Human CD11b (MCA551PE) and StarBright Yellow 800 conjugated Mouse anti Human CD10 (MCA1556SBY800). All experiments performed on red cell lysed human blood gated on live single cell granulocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD10 Antibody, clone SN5c gallery image 23
Figure A. FITC conjugated Mouse anti Human CD11b (MCA551F). Figure B. FITC conjugated Mouse anti Human CD11b (MCA551F) and StarBright Red 775 conjugated Mouse anti Human CD10 (MCA1556SBR775). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Mouse anti Human CD10

Product Type
Monoclonal Antibody
Clone
SN5c
Isotype
IgG1
Specificity
CD10

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Product Code Applications Pack Size List Price Your Price Qty
MCA1556GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP WB 0.1 mg loader
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loader
MCA1556
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP WB 0.2 mg loader
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loader

Mouse anti Human CD10 antibody, clone SN5c recognizes human neprilysin, also known as CD10, atriopeptidase, enkephalinase, neutral endopeptidase 24.11, skin fibroblast elastase or common acute lymphocytic leukemia antigen (CALLA). CD10 is a 749 aminoacid, ~100 kDa single pass type II transmembrane glycoprotein expressed by acute lymphoblastic leukaemia cells and by peripheral blood granulocytes.

Defects in the MME gene encoding CD10 can lead to the development of the peripheral nervous system disorder, Charcot-Marie-Tooth disease 2T (CMY2T), an axonal form of Marie-Charcot-Tooth disease characterized by either dominantly inherited primary peripheral demyelinating neuropathies, designated CMT1, or primary peripheral axonal neuropathies showing axonal degeneration in the absence of any obvious myelin alteration (CMT2).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
MCA1556GA: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
MCA1556: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Immunogen
Partially purified cell membrane antigens from fresh leukemia cells
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse PS/NS1/1-Ag4-1 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/100 Pack Size: 0.1 mg
1/10 Pack Size: 0.2 mg		 1/200 Pack Size: 0.1 mg
1/25 Pack Size: 0.2 mg
Immunohistology - Frozen
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG (Fc):HRP
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader
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Description Mouse IgG1 Negative Control

Source Reference

  1. Matsuzaki, H. et al. (1987) Unique epitopes of common acute lymphoblastic leukemia antigen detected by new monoclonal antibodies.
    Cancer Res. 47 (8): 2160-6.

References for CD10 antibody

  1. Biddle, W.C. et al. (1989) In vitro and in vivo cytotoxic activity of anti-human leukemia monoclonal antibodies SN5c and SN6 daunorubicin conjugates.
    Leuk Res. 13 (8): 699-707.
  2. Riemann, D. et al. (2001) Caveolae/lipid rafts in fibroblast-like synoviocytes: ectopeptidase-rich membrane microdomains.
    Biochem J. 354 (Pt 1): 47-55.
  3. Diaz-Romero, J. et al. (2008) Immunophenotypic changes of human articular chondrocytes during monolayer culture reflect bona fide dedifferentiation rather than amplification of progenitor cells.
    J Cell Physiol. 214: 75-83.
  4. Pilling, D. et al. (2009) Identification of markers that distinguish monocyte-derived fibrocytes from monocytes, macrophages, and fibroblasts.
    PLoS One. 4 (10): e7475.
  5. Manini, I. et al. (2020) Heterogeneity Matters: Different Regions of Glioblastoma Are Characterized by Distinctive Tumor-Supporting Pathways.
    Cancers (Basel). 12 (10)Oct 13 [Epub ahead of print].
  6. Glynn, E. & Fromm, J.R. (2020) Immunophenotypic Characterization and Purification of Neoplastic Cells from Lymph Nodes Involved by T-Cell/Histiocyte-rich Large B-cell Lymphoma by Flow Cytometry and Flow Cytometric Cell Sorting.
    Cytometry B Clin Cytom. 98 (1): 88-98.
  7. Caponnetto, F. et al. (2020) Human Adipose-Derived Stem Cells in Madelung's Disease: Morphological and Functional Characterization.
    Cells. 10 (1): 44.
  8. Kirolos, S.A. and Gomer, R.H. (2022) The extracellular sialidase NEU3 induces neutrophil priming
    bioRxiv. 24 Feb [Epub ahead of print].
    9. View The Latest Product References

Immunofluorescence

Synonyms
CALLA
RRID
AB_321494
UniProt
P08473
Entrez Gene
MME
GO Terms
GO:0005515 protein binding
GO:0004222 metalloendopeptidase activity
GO:0005887 integral to plasma membrane
GO:0046872 metal ion binding
GO:0006508 proteolysis
GO:0007267 cell-cell signaling

MCA1556GA

155041 165252 165731 167135

MCA1556

1113 162134

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