CD10 antibody | SN5c

Product Code Applications Pack Size List Price Your Price Qty
MCA1556GA
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SDS Safety Datasheet SDS
C F IP WB 0.1 mg loader
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loader
MCA1556
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SDS Safety Datasheet SDS
C F IP WB 0.2 mg loader
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Mouse anti Human CD10 antibody, clone SN5c recognizes human neprilysin, also known as CD10, atriopeptidase, enkephalinase, neutral endopeptidase 24.11, skin fibroblast elastase or common acute lymphocytic leukemia antigen (CALLA). CD10 is a 749 aminoacid, ~100 kDa single pass type II transmembrane glycoprotein expressed by acute lymphoblastic leukaemia cells and by peripheral blood granulocytes.

Defects in the MME gene encoding CD10 can lead to the development of the peripheral nervous system disorder, Charcot-Marie-Tooth disease 2T (CMY2T), an axonal form of Marie-Charcot-Tooth disease characterized by either dominantly inherited primary peripheral demyelinating neuropathies, designated CMT1, or primary peripheral axonal neuropathies showing axonal degeneration in the absence of any obvious myelin alteration (CMT2).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
Pack Size: 0.1 mg
0.09%Sodium Azide
Pack Size: 0.2 mg
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Partially purified cell membrane antigens from fresh leukemia cells
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse PS/NS1/1-Ag4-1 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/100 Pack Size: 0.1 mg
1/10 Pack Size: 0.2 mg
1/200 Pack Size: 0.1 mg
1/25 Pack Size: 0.2 mg
Immunohistology - Frozen
Immunoprecipitation
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Goat anti Mouse IgG (Fc):HRP
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)

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Description Mouse IgG1 Negative Control

Source Reference

  1. Matsuzaki, H. et al. (1987) Unique epitopes of common acute lymphoblastic leukemia antigen detected by new monoclonal antibodies.
    Cancer Res. 47 (8): 2160-6.

References for CD10 antibody

  1. Biddle, W.C. et al. (1989) In vitro and in vivo cytotoxic activity of anti-human leukemia monoclonal antibodies SN5c and SN6 daunorubicin conjugates.
    Leuk Res. 13 (8): 699-707.
  2. Riemann, D. et al. (2001) Caveolae/lipid rafts in fibroblast-like synoviocytes: ectopeptidase-rich membrane microdomains.
    Biochem J. 354 (Pt 1): 47-55.
  3. Diaz-Romero, J. et al. (2008) Immunophenotypic changes of human articular chondrocytes during monolayer culture reflect bona fide dedifferentiation rather than amplification of progenitor cells.
    J Cell Physiol. 214: 75-83.
  4. Pilling, D. et al. (2009) Identification of markers that distinguish monocyte-derived fibrocytes from monocytes, macrophages, and fibroblasts.
    PLoS One. 4 (10): e7475.
  5. Manini, I. et al. (2020) Heterogeneity Matters: Different Regions of Glioblastoma Are Characterized by Distinctive Tumor-Supporting Pathways.
    Cancers (Basel). 12 (10)Oct 13 [Epub ahead of print].
  6. Glynn, E. & Fromm, J.R. (2020) Immunophenotypic Characterization and Purification of Neoplastic Cells from Lymph Nodes Involved by T-Cell/Histiocyte-rich Large B-cell Lymphoma by Flow Cytometry and Flow Cytometric Cell Sorting.
    Cytometry B Clin Cytom. 98 (1): 88-98.
  7. Caponnetto, F. et al. (2020) Human Adipose-Derived Stem Cells in Madelung's Disease: Morphological and Functional Characterization.
    Cells. 10 (1): 44.
  8. Kirolos, S.A. and Gomer, R.H. (2022) The extracellular sialidase NEU3 induces neutrophil priming
    bioRxiv. 24 Feb [Epub ahead of print].
  9. View The Latest Product References

Immunofluorescence

Synonyms
CALLA
RRID
AB_321494
UniProt
P08473
Entrez Gene
MME
GO Terms
GO:0005515 protein binding
GO:0004222 metalloendopeptidase activity
GO:0005887 integral to plasma membrane
GO:0046872 metal ion binding
GO:0006508 proteolysis
GO:0007267 cell-cell signaling
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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