CCL3 antibody | H03-5C10

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Mouse anti Human CCL3

Product Type
Monoclonal Antibody
Clone
H03-5C10
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA6220GA
Datasheet
E
SDS
0.1 mg loader

Mouse anti Human CCL3 antibody, clone H03-5C10, recognizes C-C motif chemokine 3 (CCL3) also known as G0/G1 switch regulatory protein 19-1 and Macrophage inflammatory protein 1 alpha (MIP1α). CCL3 is an inflammatory cytokine with the ability to activate chemotactic mobilization of monocyte and lymphocyte cells. It also plays a role in regulating the proliferation of hematopoietic stem/progenitor cells (Baba & Mukaida 2014). The receptors of CCL3 are the G-protein coupled receptors CCR1, CCR5 and the D6 receptor in humans. Signaling via CCR1 and CCR5, CCL3 is able to stimulate lymphocyte recruitment to sites of infection, as well as CD8+ T cells to active lymph nodes (Staversky et al. 2018).

Expression levels of CCL3 have been reported to increase in patients with colorectal cancer, independently of tumor stage. This has been shown to be concurrent with the upregulated expression of CCR1 and CCR5 (Lόpez et al. 2018). CCL3 has also been implicated in various pathologies related to microenvironmental dysfunction, including myelodysplastic syndrome, metastatic disease of renal cell carcinoma and B cell lymphomas (Staversky et al. 2018).

The purified Mouse anti Human CCL3 antibody, clone H03-5C10 (MCA6220GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human CCL3 antibody, clone F01.1-3E5 (MCA6221B) as the detection antibody.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
E. coli derived recombinant human full length CCL3 (Ala27-Ala92)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P10147
Entrez Gene
CCL3
GO Terms
GO:0007010 cytoskeleton organization
GO:0004871 signal transducer activity
GO:0005615 extracellular space
GO:0005625 soluble fraction
GO:0006874 cellular calcium ion homeostasis
GO:0006887 exocytosis
GO:0006928 cellular component movement
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0007267 cell-cell signaling
GO:0008009 chemokine activity
GO:0042056 chemoattractant activity
GO:0045069 regulation of viral genome replication
Regulatory
For research purposes only

Applications of CCL3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Human CCL3:Biotin MCA6221B E 0.1 mg loader

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