C7 antibody | 030-113.7.5.4

100% Secure

Mouse anti Human C7

Product Type
Monoclonal Antibody
Clone
030-113.7.5.4
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2644 E FN WB 0.1 mg
Mouse anti Human C7 antibody, clone 030-113.7.5.4 recognises complement component 7 (C7), a 110 kDa glycoprotein present in the blood serum. The factor I domain of C7 binds the C terminus of the C5 alpha-chain, acting as a membrane anchor. This enables the assembly of the complement membrane attack complex (MAC) and consequent complement lytic activity. C7 deficiency is associated with increased susceptibility to recurrent infections, in particular by Neisseria meningitides.

Mouse anti Human C7 antibody, clone 030-113.7.5.4 does not recognie membrane bound complement membrane attack complex.

Mouse anti Human C7 antibody, clone 030-113.7.5.4 inhibits lysis of sensitised sheep erythrocytes.

Removal of Sodium Azide is recommended prior to use in functional assays. The use of EQU003 is recommended for this purpose.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Borate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Immunogen
Purified human C7.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P10643 Related reagents
Entrez Gene
C7 Related reagents
GO Terms
GO:0005576 extracellular region
GO:0005579 membrane attack complex
GO:0006957 complement activation, alternative pathway
GO:0006958 complement activation, classical pathway
GO:0019835 cytolysis
Regulatory
For research purposes only

Applications of C7 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Functional Assays
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Product Specific References

References for C7 antibody

  1. Langer, F. et al. (2013) Rapid activation of monocyte tissue factor by antithymocyte globulin is dependent on complement and protein disulfide isomerase.
    Blood. 121 (12): 2324-35.

Further Reading

  1. Thai, C.T. & Ogata, R.T. (2004) Complement components C5 and C7: recombinant factor I modules of C7 bind to the C345C domain of C5.
    J Immunol. 173 (7): 4547-52.
  2. Debard, A.L. et al. (2005) FcgammaRIIIb and complement component C7 codeficiency in a patient with recurrence of fulminant meningococcal septic shock.
    Clin Infect Dis. 40 (11): 1679-83.