C5a/C5a Desarg (neo-epitope) antibody | 2952
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Mouse anti Human C5a/C5a Desarg (NEO-EPITOPE)
- Product Type
- Monoclonal Antibody
- Clone
- 2952
- Isotype
- IgG1
- Specificity
- C5a/C5a Desarg (neo-epitope)
Antibody Quality and Performance Guarantee
| Mouse anti Human C5a/C5a desArg (neo-epitope) antibody, clone 2952, recognizes a neo-epitope on the human complement proteins C5a and C5a desArg. Neo-epitopes are regions on native proteins, like C5, that are normally only exposed and detectable by monoclonal antibodies, on active forms of the protein. C5 is a member of the complement cascade and all three activation pathways (classical, lectin and alternative), lead to its cleavage. Upon activation of any of these pathways, C5 is cleaved by C5 convertases into C5b, which mediates pathogen lysis, and the inflammatory mediator C5a. The inflammatory responses mediated by C5a are chemotaxis, leukocyte degranulation, vascular permeability and cytokine plus chemokine production. Beyond innate immunity though, C5a plays a role in organ development, tissue regeneration and hematopoiesis (Zhang et al. 2017). C5a elicits these responses through its interaction with the receptors C5aR and C5aR (C5L2). These receptors are expressed ubiquitously, but are found in higher levels on the surface of immune cells, including macrophages, neutrophils and T cells. However, C5a is a short lived in circulation and is cleaved at its C-terminal arginine, by carboxypeptidases, generating the more stable C5a desArg. While C5a desArg is more stable in serum than C5a, it is less potent in comparison, but they do activate a similar range of biological responses (Zhang et al. 2017, Merle et al. 2015). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- ISHKDMQLG (C5a amino acid 65-73)
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Immunohistology - Frozen | |
||
| Immunohistology - Paraffin | |
1/100 | |
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- UniProt
- P01031
- Entrez Gene
- C5
- GO Terms
- GO:0000187 activation of MAPK activity
- GO:0004866 endopeptidase inhibitor activity
- GO:0005579 membrane attack complex
- GO:0005615 extracellular space
- GO:0006935 chemotaxis
- GO:0006954 inflammatory response
- GO:0006957 complement activation, alternative pathway
- GO:0006958 complement activation, classical pathway
- GO:0007186 G-protein coupled receptor protein signaling pathway
- View More GO Terms
- GO:0008009 chemokine activity
- GO:0019835 cytolysis
- GO:0010575 positive regulation vascular endothelial growth factor production
- GO:0010760 negative regulation of macrophage chemotaxis
- GO:0090197 positive regulation of chemokine secretion
MCA6155GA
157928
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