Mouse anti Human C5/C5b antibody, clone 568,
recognizes an epitope on C5 and C5b. C5 is a component of all three complement activation pathways (classical, lectin and alternative), with all leading to its cleavage. C5 convertases cleave C5 into the anaphylatoxin C5a and C5b. The creation of C5b initiates the creation of the membrane attack complex (C5b-9) resulting in pathogenic cell lysis. This is achieved through the incorporation of the complex into bacterial cell walls, forming a pore, particularly in gram negative Neisseria strains (Merle et al. 2015
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
activation of MAPK activity
endopeptidase inhibitor activity
membrane attack complex
complement activation, alternative pathway
complement activation, classical pathway
G-protein coupled receptor protein signaling pathway
positive regulation vascular endothelial growth factor production
negative regulation of macrophage chemotaxis
positive regulation of chemokine secretion
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of C5/C5b antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
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