C5/C5a antibody | 557
Mouse anti Human C5/C5a
- Product Type
- Monoclonal Antibody
- Clone
- 557
- Isotype
- IgG2a
- Specificity
- C5/C5a
Mouse anti Human C5/C5a antibody, clone 557, recognizes an epitope on C5 and C5a. C5 is a component of all three complement activation pathways (classical, lectin and alternative), which lead to its cleavage by C5 convertase. Cleavage of C5 results in the formation of C5b, which initiates pathogenic lysis and C5a, a potent inflammatory mediator. C5a is a ligand for the G protein coupled receptors, C5aR1 and C5aR2 (also known as C5L2) through which its inflammatory functions are controlled (Merle et al. 2015). These receptors are expressed ubiquitously but are found at its highest levels on the surface of immune cells, including macrophages, neutrophils and T cells. The inflammatory responses mediated by C5a are chemotaxis, leukocyte degranulation, vascular permeability and cytokine plus chemokine production. Beyond innate immunity, C5a plays a role in organ development, tissue regeneration and hematopoiesis (Zhang et al. 2017). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Human C5
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 1/50 | ||
Western Blotting |
- UniProt
- P01031
- Entrez Gene
- C5
- GO Terms
- GO:0000187 activation of MAPK activity
- GO:0004866 endopeptidase inhibitor activity
- GO:0005579 membrane attack complex
- GO:0005615 extracellular space
- GO:0006935 chemotaxis
- GO:0006954 inflammatory response
- GO:0006957 complement activation, alternative pathway
- GO:0006958 complement activation, classical pathway
- GO:0007186 G-protein coupled receptor protein signaling pathway
- View More GO Terms
- GO:0008009 chemokine activity
- GO:0019835 cytolysis
- GO:0010575 positive regulation vascular endothelial growth factor production
- GO:0010760 negative regulation of macrophage chemotaxis
- GO:0090197 positive regulation of chemokine secretion
MCA6156GA
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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