Mouse anti Human C3d antibody, clone 053A-1184.108.40.206 recognizes human complement component 3d (C3d), a ~33 kDa polypeptide fragment generated over the course of complement activation where complement 3 (C3) convertases cleave C3 to C3b, which is further degraded into iC3b and C3dg/C3d.
C3d is involved in the regulation of many aspects of the immune response, including antigen processing and presentation. The C3d/antigen complex mediates B cell activation by simultaneously binding antigen-specific surface bound immunoglobulin and CD21, lowering the complement activation threshold. C3 is crucial in the induction of tolerance generated when an antigen is introduced into immunoprivileged sites and this is exploited by pathogens and cancer cells to evade the immune system by inhibiting complement activation.
- Target Species
- Product Form
- Purified Ig - liquid
- Purified Ig prepared by affinity chromatography on Protein A.
- Buffer Solution
- Borate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Purified C3d from human plasma.
- Approx. Protein Concentrations
- Please see label for concentration
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Guaranteed until date of expiry. Please see product label.
- Entrez Gene
- GO Terms
endopeptidase inhibitor activity
complement activation, alternative pathway
complement activation, classical pathway
G-protein coupled receptor protein signaling pathway
positive regulation vascular endothelial growth factor production
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of C3d antibody
|Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- For best results Bio-Rad recommend HISTAR detection kits.
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Secondary Antibodies Available
Negative Isotype Controls Available
Useful Reagents Available
Product Specific References
References for C3d antibody
Gay, F. (2013) Staphylococcal immune complexes and myelinolytic toxin in early acute multiple sclerosis lesions-An immunohistological study supported by multifactorial cluster analysis and antigen-imprint isoelectric focusing.
Mult Scler Relat Disord. 2 (3): 213-32.
Fujieda, M. et al. (2012) L-ficolin and capsular polysaccharide-specific IgG in cord serum contribute synergistically to opsonophagocytic killing of serotype III and V group B streptococci.
Infect Immun. 80 (6): 2053-60.
Bergmann-Leitner, E.S. et al. (2006) Complement 3d: from molecular adjuvant to target of immune escape mechanisms.
Clin Immunol. 121 (2): 177-85.