C3c antibody | 013E-498.2.3 (10-02A)

Mouse anti Human C3c

Product Type
Monoclonal Antibody
Clone
013E-498.2.3 (10-02A)
Isotype
IgG1
Specificity
C3c

Product Code Applications Pack Size List Price Your Price Qty
MCA2605
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E * F WB 0.1 mg
List Price Your Price
Search for Batch Specific Datasheets

Mouse anti human C3c, clone 013E-498.2.3 (10-02A), recognizes the ~135 kDa complement component 3c present in serum. C3c is generated over the course of complement activation, where convertase C4b2a (classical pathway) and convertase C3bBb (alternative pathway) cleave C3 to C3b and C3a. C3b is further degraded into iC3b and C3dg/C3d. iC3b is then slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f.

C3 is crucial in the induction of tolerance generated when an antigen is introduced into immunoprivileged sites and this is exploited by pathogens and cancer cells to evade the immune system by inhibiting complement activation.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from ascites
Buffer Solution
Borate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Immunogen
Human C3 from normal serum, highly purified by chromatography.
Approx. Protein Concentrations
Current, batch-specific concentration 1.2 mg/ml
Regulatory
For research purposes only
Guarantee
Guaranteed until date of expiry. Please see product label.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1
Flow Cytometry
Immunohistology - Frozen
Western Blotting
  1. 1Clone 10-02A binds both C3c and C3 and so is not suitable for all ELISA applications.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Kidney from post streptococal glomerulonephritis patients.

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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)

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References for C3c antibody

  1. Sica, M. et al. (2017) Eculizumab treatment: stochastic occurrence of C3 binding to individual PNH erythrocytes.
    J Hematol Oncol. 10 (1): 126.

Further Reading

  1. Janssen, B.J. et al. (2005) Structures of complement component C3 provide insights into the function and evolution of immunity.
    Nature. 437 (7058): 505-11.

Synonyms
Complement Component 3c
RRID
AB_844526
UniProt
P01024
Entrez Gene
C3
GO Terms
GO:0005102 receptor binding
GO:0004866 endopeptidase inhibitor activity
GO:0005615 extracellular space
GO:0006954 inflammatory response
GO:0006957 complement activation, alternative pathway
GO:0006958 complement activation, classical pathway
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0010575 positive regulation vascular endothelial growth factor production
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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