C3a Receptor antibody | hC3aRZ1

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Mouse anti Human C3a Receptor:Alexa Fluor® 488

Mouse anti Human C3a Receptor:Alexa Fluor® 647

Mouse anti Human C3a Receptor

Product Type
Monoclonal Antibody
Clone
hC3aRZ1
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2455A488 F 100 Tests/1ml
MCA2455A488T F 25 Tests/0.25ml
MCA2455A647 F 100 Tests/1ml
MCA2455A647T F 25 Tests/0.25ml
MCA2455GA C F 0.1 mg
Mouse anti Human C3a Receptor antibody, clone hC3aRZ1 recognizes the receptor for human complement component C3a (C3aR), a ~55 kDa cell surface protein of the rhodopsin family of G protein-coupled seven-transmembrane receptors. C3a is an anaphylatoxin, which is a potent proinflammatory mediator that possesses immunoregulatory properties. C3a regulates inflammatory functions by binding to the C3a receptor. C3aR is expressed on various tissues and leucocytes including basophils, neutrophils, mast cells, eosinophils and activated T lymphocytes.

Expression levels of C3aR on human cells varies significantly between donors.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Alexa Fluor®488 - liquid
Product Form
Purified IgG conjugated to Alexa Fluor® 647- liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Recombinant human C3aR protein.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Cells from immunised BALB/c mice were fused with cells of the X63-Ag8.653 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q16581 Related reagents
Entrez Gene
C3AR1 Related reagents
GO Terms
GO:0004435 phosphatidylinositol phospholipase C activity
GO:0004876 complement component C3a receptor activity
GO:0004943 C3a anaphylatoxin receptor activity
GO:0005887 integral to plasma membrane
GO:0006935 chemotaxis
GO:0006954 inflammatory response
GO:0007204 elevation of cytosolic calcium ion concentration
GO:0008015 blood circulation
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of C3a Receptor antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Flow Cytometry Neat
Flow Cytometry 1/10 1/20
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the sugested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the sugested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the sugested working dilution to label 1x106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 488 MCA928A488 100 Tests/1ml F
Mouse IgG1 Negative Control:Alexa Fluor® 647 MCA928A647 100 Tests/1ml F
Mouse IgG1 Negative Control MCA928 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Application Based External Images

Flow Cytometry

Product Specific References

References for C3a Receptor antibody

  1. Werfel, T. et al. (2000) Activated human T lymphocytes express a functional C3a receptor.
    J Immunol. 165 (11): 6599-605.
  2. Soruri, A. et al. (2003) IL-4 down-regulates anaphylatoxin receptors in monocytes and dendritic cells and impairs anaphylatoxin-induced migration in vivo.
    J Immunol. 170 (6): 3306-14.
  3. Zwirner, J. et al. (1999) Evaluation of C3a receptor expression on human leucocytes by the use of novel monoclonal antibodies.
    Immunology. 97: 166-72.
  4. Kacani, L. et al. (2001) C5a and C5a(desArg) enhance the susceptibility of monocyte-derived macrophages to HIV infection.
    J Immunol. 166: 3410-5.
  5. Raby, A.C. et al. (2009) Soluble TLR2 reduces inflammation without compromising bacterial clearance by disrupting TLR2 triggering.
    J Immunol. 183: 506-17.
  6. Martel, C. et al. (2011) Requirements for membrane attack complex formation and anaphylatoxins binding to collagen-activated platelets.
    PLoS One. 6: e18812.
  7. Hengartner, N.E. et al. (2015) Crucial Role of IL1beta and C3a in the In Vitro-Response of Multipotent Mesenchymal Stromal Cells to Inflammatory Mediators of Polytrauma.
    PLoS One. 10: e0116772.
  8. Schreiber, A. et al. (2009) C5a receptor mediates neutrophil activation and ANCA-induced glomerulonephritis.
    J Am Soc Nephrol. 20: 289-98.
  9. Török K (2012) Human T cell derived, cell-bound complement iC3b is integrally involved in T cell activation.
    Immunol Lett. 143: 131-6.
  10. Patzelt, J. et al. (2015) Expression of anaphylatoxin receptors on platelets in patients with coronary heart disease.
    Atherosclerosis. 238 (2): 289-95.
  11. Raby, A.C. et al. (2009) Soluble TLR2 reduces inflammation without compromising bacterial clearance by disrupting TLR2 triggering.
    J Immunol. 183 (1): 506-17.