C3a Receptor antibody | hC3aRZ1
Mouse anti Human C3a receptor antibody, clone hC3aRZ1 used for flow cytometry.
Image caption
Expression of C3aR1 and C5aR on collagen- and convulxin-activated platelets. Washed platelets were activated for 10 min with 2.5 µg/mL collagen, 500 ng/mL convulxin or saline and further incubated for 30 min at RT and analyzed for C3aR1 and C5aR expression by flow cytometry. A: representative overlays of fluorescence histograms obtained for each condition using an alexa fluor 488 anti-C3aR1 (solid line), a FITC anti-CD88 (dashed line) or a FITC control IgG (dotted line). B: Mean fluorescence intensities ± SEM of 3 to 9 experiments for each condition.
From: Martel C, Cointe S, Maurice P, Matar S, Ghitescu M, et al. (2011)
Requirements for Membrane Attack Complex Formation and Anaphylatoxins Binding to Collagen-Activated Platelets.
PLoS ONE 6(4): e18812.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Filter by Application:
F ResetMouse anti Human C3a Receptor
- Product Type
- Monoclonal Antibody
- Clone
- hC3aRZ1
- Isotype
- IgG1
- Specificity
- C3a Receptor
Antibody Quality and Performance Guarantee
| Mouse anti Human C3a Receptor antibody, clone hC3aRZ1 recognizes the receptor for human complement component C3a (C3aR), a ~55 kDa cell surface protein of the rhodopsin family of G protein-coupled seven-transmembrane receptors. C3a is an anaphylatoxin, which is a potent proinflammatory mediator that possesses immunoregulatory properties. C3a regulates inflammatory functions by binding to the C3a receptor. C3aR is expressed on various tissues and leucocytes including basophils, neutrophils, mast cells, eosinophils and activated T lymphocytes. Expression levels of C3aR on human cells varies significantly between donors. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Recombinant human C3aR protein.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Cells from immunised BALB/c mice were fused with cells of the X63-Ag8.653 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/10 | 1/20 |
| Immunohistology - Frozen | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the sugested working dilution to label 1x106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for C3a Receptor antibody
-
Werfel, T. et al. (2000) Activated human T lymphocytes express a functional C3a receptor.
J Immunol. 165 (11): 6599-605. -
Soruri, A. et al. (2003) IL-4 down-regulates anaphylatoxin receptors in monocytes and dendritic cells and impairs anaphylatoxin-induced migration in vivo.
J Immunol. 170 (6): 3306-14. -
Zwirner, J. et al. (1999) Evaluation of C3a receptor expression on human leucocytes by the use of novel monoclonal antibodies.
Immunology. 97: 166-72. -
Kacani, L. et al. (2001) C5a and C5a(desArg) enhance the susceptibility of monocyte-derived macrophages to HIV infection.
J Immunol. 166: 3410-5. -
Raby, A.C. et al. (2009) Soluble TLR2 reduces inflammation without compromising bacterial clearance by disrupting TLR2 triggering.
J Immunol. 183: 506-17. -
Martel, C. et al. (2011) Requirements for membrane attack complex formation and anaphylatoxins binding to collagen-activated platelets.
PLoS One. 6: e18812. -
Hengartner, N.E. et al. (2015) Crucial Role of IL1beta and C3a in the In Vitro-Response of Multipotent Mesenchymal Stromal Cells to Inflammatory Mediators of Polytrauma.
PLoS One. 10: e0116772. -
Schreiber, A. et al. (2009) C5a receptor mediates neutrophil activation and ANCA-induced glomerulonephritis.
J Am Soc Nephrol. 20: 289-98.
View The Latest Product References
-
Török K (2012) Human T cell derived, cell-bound complement iC3b is integrally involved in T cell activation.
Immunol Lett. 143: 131-6. -
Patzelt, J. et al. (2015) Expression of anaphylatoxin receptors on platelets in patients with coronary heart disease.
Atherosclerosis. 238 (2): 289-95. -
Raby, A.C. et al. (2009) Soluble TLR2 reduces inflammation without compromising bacterial clearance by disrupting TLR2 triggering.
J Immunol. 183 (1): 506-17.
- Synonyms
- Complement Component 3a Receptor 1
- RRID
- AB_877450
- UniProt
- Q16581
- Entrez Gene
- C3AR1
- GO Terms
- GO:0004435 phosphatidylinositol phospholipase C activity
- GO:0004876 complement component C3a receptor activity
- GO:0004943 C3a anaphylatoxin receptor activity
- GO:0005887 integral to plasma membrane
- GO:0006935 chemotaxis
- GO:0006954 inflammatory response
- GO:0007204 elevation of cytosolic calcium ion concentration
- GO:0008015 blood circulation
MCA2455GA
159380
1708
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