C3a Receptor antibody | hC3aRZ1

Mouse anti Human C3a Receptor

Product Type
Monoclonal Antibody
Clone
hC3aRZ1
Isotype
IgG1
Specificity
C3a Receptor

Product Code Applications Pack Size List Price Your Price Qty
MCA2455GA
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SDS Safety Datasheet SDS
C F 0.1 mg loader
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loader
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Mouse anti Human C3a Receptor antibody, clone hC3aRZ1 recognizes the receptor for human complement component C3a (C3aR), a ~55 kDa cell surface protein of the rhodopsin family of G protein-coupled seven-transmembrane receptors. C3a is an anaphylatoxin, which is a potent proinflammatory mediator that possesses immunoregulatory properties. C3a regulates inflammatory functions by binding to the C3a receptor. C3aR is expressed on various tissues and leucocytes including basophils, neutrophils, mast cells, eosinophils and activated T lymphocytes.

Expression levels of C3aR on human cells varies significantly between donors.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Recombinant human C3aR protein.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Cells from immunised BALB/c mice were fused with cells of the X63-Ag8.653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/20
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the sugested working dilution to label 1x106 cells in 100ul.

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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG (Fc):HRP
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
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Description Mouse IgG1 Negative Control

References for C3a Receptor antibody

  1. Werfel, T. et al. (2000) Activated human T lymphocytes express a functional C3a receptor.
    J Immunol. 165 (11): 6599-605.
  2. Soruri, A. et al. (2003) IL-4 down-regulates anaphylatoxin receptors in monocytes and dendritic cells and impairs anaphylatoxin-induced migration in vivo.
    J Immunol. 170 (6): 3306-14.
  3. Zwirner, J. et al. (1999) Evaluation of C3a receptor expression on human leucocytes by the use of novel monoclonal antibodies.
    Immunology. 97: 166-72.
  4. Kacani, L. et al. (2001) C5a and C5a(desArg) enhance the susceptibility of monocyte-derived macrophages to HIV infection.
    J Immunol. 166: 3410-5.
  5. Raby, A.C. et al. (2009) Soluble TLR2 reduces inflammation without compromising bacterial clearance by disrupting TLR2 triggering.
    J Immunol. 183: 506-17.
  6. Martel, C. et al. (2011) Requirements for membrane attack complex formation and anaphylatoxins binding to collagen-activated platelets.
    PLoS One. 6: e18812.
  7. Hengartner, N.E. et al. (2015) Crucial Role of IL1beta and C3a in the In Vitro-Response of Multipotent Mesenchymal Stromal Cells to Inflammatory Mediators of Polytrauma.
    PLoS One. 10: e0116772.
  8. Schreiber, A. et al. (2009) C5a receptor mediates neutrophil activation and ANCA-induced glomerulonephritis.
    J Am Soc Nephrol. 20: 289-98.
  9. View The Latest Product References
  10. Török K (2012) Human T cell derived, cell-bound complement iC3b is integrally involved in T cell activation.
    Immunol Lett. 143: 131-6.
  11. Patzelt, J. et al. (2015) Expression of anaphylatoxin receptors on platelets in patients with coronary heart disease.
    Atherosclerosis. 238 (2): 289-95.
  12. Raby, A.C. et al. (2009) Soluble TLR2 reduces inflammation without compromising bacterial clearance by disrupting TLR2 triggering.
    J Immunol. 183 (1): 506-17.

Flow Cytometry

Synonyms
Complement Component 3a Receptor 1
RRID
AB_877450
UniProt
Q16581
Entrez Gene
C3AR1
GO Terms
GO:0004435 phosphatidylinositol phospholipase C activity
GO:0004876 complement component C3a receptor activity
GO:0004943 C3a anaphylatoxin receptor activity
GO:0005887 integral to plasma membrane
GO:0006935 chemotaxis
GO:0006954 inflammatory response
GO:0007204 elevation of cytosolic calcium ion concentration
GO:0008015 blood circulation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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