C3a/C3a Desarg antibody | 2991
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Mouse anti Human C3a/C3a Desarg
- Product Type
- Monoclonal Antibody
- Clone
- 2991
- Isotype
- IgG1
- Specificity
- C3a/C3a Desarg
Antibody Quality and Performance Guarantee
| Mouse anti Human C3a/C3a desArg antibody, clone 2991, recognizes the neo-epitope of both human C3a and C3a desArg, with at least a 5x higher preference for C3a desArg. Neo-epitopes are regions on native proteins that are only exposed and detectable on activated proteins. As such, detection of these neo-epitopes can be used for the direct quantification of the activation of different steps in the complement cascade. C3 is a 185 kDa glycoprotein consisting of an alpha and beta chain and is the key component of the complement activation pathways. There are 3 activation pathways within the complement system; the classical, lectin and alternative pathways. All three pathways converge on C3; resulting in its cleavage to anaphylatoxin C3a and opsonin C3b (Dunkelberger & Song 2010). C3a is a ligand to the seven-transmembrane coupled receptor C3aR. Upon binding to this receptor C3a elicits an anti-inflammatory response, by attenuating neutrophil mobilization into circulation after injury (Mathern & Heeger 2015). C3a is however rapidly cleaved by serum carboxypeptidases at the C-terminal arginine to the more stable C3a desArg. C3a desArg has no detectable activity with C3aR, but there is some evidence to suggest that it can bind to C5aR2, a receptor of C5a as well as C5L2 (Kalant et al. 2003, Zhang et al. 2017). Measurement of C3a desArg has been used to examine the level of complement activation in test samples. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Human C3a des-arg
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- UniProt
- P01024
- Entrez Gene
- C3
- GO Terms
- GO:0005102 receptor binding
- GO:0004866 endopeptidase inhibitor activity
- GO:0005615 extracellular space
- GO:0006954 inflammatory response
- GO:0006957 complement activation, alternative pathway
- GO:0006958 complement activation, classical pathway
- GO:0007186 G-protein coupled receptor protein signaling pathway
- GO:0010575 positive regulation vascular endothelial growth factor production
MCA6163GA
157935
161140
166406
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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