C3a/C3a Desarg antibody | 2991

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Mouse anti Human C3a/C3a Desarg

Product Type
Monoclonal Antibody
Clone
2991
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA6163GA
Datasheet
E WB
SDS
0.1 mg loader

Mouse anti Human C3a/C3a desArg antibody, clone 2991, recognizes the neo-epitope of both human C3a and C3a desArg, with at least a 5x higher preference for C3a desArg. Neo-epitopes are regions on native proteins that are only exposed and detectable on activated proteins. As such, detection of these neo-epitopes can be used for the direct quantification of the activation of different steps in the complement cascade.

C3 is a 185 kDa glycoprotein consisting of an alpha and beta chain and is the key component of the complement activation pathways. There are 3 activation pathways within the complement system; the classical, lectin and alternative pathways. All three pathways converge on C3; resulting in its cleavage to anaphylatoxin C3a and opsonin C3b (Dunkelberger & Song 2010). C3a is a ligand to the seven-transmembrane coupled receptor C3aR. Upon binding to this receptor C3a elicits an anti-inflammatory response, by attenuating neutrophil mobilization into circulation after injury (Mathern & Heeger 2015). C3a is however rapidly cleaved by serum carboxypeptidases at the C-terminal arginine to the more stable C3a desArg. C3a desArg has no detectable activity with C3aR, but there is some evidence to suggest that it can bind to C5aR2, a receptor of C5a as well as C5L2 (Kalant et al. 2003, Zhang et al. 2017). Measurement of C3a desArg has been used to examine the level of complement activation in test samples.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Human C3a des-arg
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P01024
Entrez Gene
C3
GO Terms
GO:0005102 receptor binding
GO:0004866 endopeptidase inhibitor activity
GO:0005615 extracellular space
GO:0006954 inflammatory response
GO:0006957 complement activation, alternative pathway
GO:0006958 complement activation, classical pathway
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0010575 positive regulation vascular endothelial growth factor production
Regulatory
For research purposes only

Applications of C3a/C3a Desarg antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

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