C3a antibody | H13

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Mouse anti Human C3a

Product Type
Monoclonal Antibody
Clone
H13
Isotype
IgG2b
Product Code Applications Pack Size List Price Quantity
0200-0615
Datasheet
C E WB
MSDS
50 µg loader

Mouse anti Human C3a antibody, clone H13 recognizes human C3a anaphylatoxin, a cleavage product of human complement component C3. C3a increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.

Mouse anti Human C3a antibody, clone H13 recognizes a C3a-determinant also present on native C3. Mouse anti Human C3a antibody, clone H13 allows discrimination of ubiquitous or non-specifically bound native C3 or C3a from biologically active C3b fragment, neither C3b or C5 components are recognized by Mouse anti Human C3a antibody, clone H13 (Burger et al. 1987).

Mouse anti Human C3a antibody, clone H13 is reported to block C3a functional activity (Hartmann et al. 1997).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Guinea Pig
Rabbit
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 1.0ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide, 0.5% Bovine Serum Albumin.
Immunogen
Human complement component C3.
Approx. Protein Concentrations
IgG concentration 50 ug/ml after reconstitution.
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells of the non-secreting P3-X63-Ag8.653 myeloma cell line.

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
Guaranteed until date of expiry. Please see product label.

More Information

UniProt
P01024 Related reagents
Entrez Gene
C3 Related reagents
GO Terms
GO:0005102 receptor binding
GO:0004866 endopeptidase inhibitor activity
GO:0005615 extracellular space
GO:0006954 inflammatory response
GO:0006957 complement activation, alternative pathway
GO:0006958 complement activation, classical pathway
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0010575 positive regulation vascular endothelial growth factor production
Regulatory
For research purposes only

Applications of C3a antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Frozen 1/10
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgG2b:FITC HCA038F F 0.1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Product Specific References

Source Reference

  1. Burger, R. et al. (1987) Functional analysis and quantification of the complement C3 derived anaphylatoxin C3a with a monoclonal antibody.
    Clin Exp Immunol. 68 (3): 703-11.

References for C3a antibody

  1. Burger, R. et al. (1988) The C terminus of the anaphylatoxin C3a generated upon complement activation represents a neoantigenic determinant with diagnostic potential.
    J Immunol. 141 (2): 553-8.
  2. Hartmann, K. et al. (1997) C3a and C5a stimulate chemotaxis of human mast cells.
    Blood. 89 (8): 2863-70.
  3. Möller, T. et al. (1997) Mechanisms of C5a and C3a complement fragment-induced [Ca2+]i signaling in mouse microglia.
    J Neurosci. 17 (2): 615-24.