C3/C3b antibody | 755
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Mouse anti Human C3/C3b
- Product Type
- Monoclonal Antibody
- Clone
- 755
- Isotype
- IgG1
- Specificity
- C3/C3b
Antibody Quality and Performance Guarantee
| Mouse anti Human C3/C3b antibody, clone 755, recognizes an epitope within the C-terminal 360 amino acids on the alpha chain of C3 and recognizes both C3b and C3. C3 is a 185 kDa glycoprotein consisting of an alpha and beta chain and is the key component of the complement activation pathways. It is also the most abundant complement protein within serum, with levels of ~1.3 mg/ml (Sim et al. 1981). There are 3 activation pathways within the complement system; the classical, lectin and alternative pathways. All three pathways converge on C3, resulting in its cleavage to anaphylatoxin C3a and opsonin C3b. C3b targets microbial cell surface receptors, marking them for phagocytosis by macrophages (Fournis et al. 2019). C3b and its degradation products also play a role in the removal of complement-coated apoptotic and necrotic cells (Xu et al. 2008). In what is considered to be a consequence of this functionality, patients with C3 deficiencies are subject to recurrent bacterial infections (Tedesco 2008). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Native human C3
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Functional Assays | |||
| Immunohistology - Frozen | |
1/50 | |
| Immunohistology - Paraffin | |
1/200 | |
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- UniProt
- P01024
- Entrez Gene
- C3
- GO Terms
- GO:0005102 receptor binding
- GO:0004866 endopeptidase inhibitor activity
- GO:0005615 extracellular space
- GO:0006954 inflammatory response
- GO:0006957 complement activation, alternative pathway
- GO:0006958 complement activation, classical pathway
- GO:0007186 G-protein coupled receptor protein signaling pathway
- GO:0010575 positive regulation vascular endothelial growth factor production
MCA6151GA
157924
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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