C1s antibody | M81
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Mouse anti Human C1s
- Product Type
- Monoclonal Antibody
- Clone
- M81
- Isotype
- IgG1
- Specificity
- C1s
Antibody Quality and Performance Guarantee
| Mouse anti Human C1s antibody, clone M81, recognizes human C1s, a subcomponent of complement component 1 (C1). This antibody recognizes an epitope around the binding site of C1s, in the IV domain and/or the V of the gamma domain and detects active and inactive C1s. C1 is a large inactive plasma complex containing one subunit of C1q, 2 subunits of C1r and 2 C1s subunits, which are non-covalently bound together. C1 is the first responder in the classical activation pathway in the complement cascade (Noris & Remuzzi 2013). The classical pathway is initiated when the C1q subunit comes into close proximity of the Fc regions of immunoglobulin’s that have bound close together to an antigen fixed on the surface of a pathogen. This binding by C1q results in the auto-activation of C1r which subsequently releases active C1s units. The newly released active C1s cleaves C4 and C2 creating C4b and C2a which then help to generate C3 convertase to continue the cascade (Janeway et al. 2001). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
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| Flow Cytometry | |
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| Immunohistology - Frozen | |
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| Immunohistology - Paraffin | |
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| Immunoprecipitation | |
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| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
MCA6159GA
157931
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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