Brain Natriuretic Peptide antibody | 50E1

Mouse anti Human Brain Natriuretic Peptide

Product Type
Monoclonal Antibody
Clone
50E1
Isotype
IgG1
Specificity
Brain Natriuretic Peptide

Product Code Applications Pack Size List Price Your Price Qty
MCA2642
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SDS Safety Datasheet SDS
E IY WB 0.2 mg loader
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loader
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Mouse anti Human Brain Natriuretic Peptide antibody, clone 50E1 detects an epitope located at the C-terminal (amino acids 26-32) of the human brain natriuretic peptide (BNP), a secreted protein which is a member of the natriuretic peptide family. BNP is a cardiac hormone, which is synthesised as a pro-hormone (proBNP), and is proteolytically cleaved to release a biologically active fragment (BNP), and an inactive fragment (NT-proBNP) into the circulation.

BNP is predominantly secreted from the cardiac ventricles in response to volume and pressure overload, and results in a number of biological activities including natriuresis, diuresis, vasorelaxation, and inhibition of the sympathetic nervous system. A high concentration of BNP in the bloodstream is indicative of heart failure.

Mouse anti Human Brain Natriuretic Peptide antibody, clone 50E1 also detects proBNP.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Human synthetic Brain natriuretic hormone.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunoassay
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
MCA2642 detects a band of approximately 4kDa when using synthetic BNP and a band of 15kDa when using recombinant proBNP.

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References for Brain Natriuretic Peptide antibody

  1. Seferian, K.R. et al. (2007) The brain natriuretic peptide (BNP) precursor is the major immunoreactive form of BNP in patients with heart failure.
    Clin Chem. 53 (5): 866-73.
  2. Tamm, N.N. et al. (2008) Novel immunoassay for quantification of brain natriuretic peptide and its precursor in human blood.
    Clin Chem. 54 (9): 1511-8.

Further Reading

  1. Clerico, A. et al. (2006) Clinical relevance of biological variation: the lesson of brain natriuretic peptide (BNP) and NT-proBNP assay.
    Clin Chem Lab Med. 44 (4): 366-78.
  2. Pfister, R. & Schneider, C.A. (2004) Natriuretic peptides BNP and NT-pro-BNP: established laboratory markers in clinical practice or just perspectives?
    Clin Chim Acta. 349 (1-2): 25-38.

RRID
AB_2155429
UniProt
P16860
Entrez Gene
NPPB
GO Terms
GO:0005615 extracellular space
GO:0006182 cGMP biosynthetic process
GO:0007168 receptor guanylyl cyclase signaling pathway
GO:0008217 regulation of blood pressure
GO:0008613 diuretic hormone activity
GO:0016525 negative regulation of angiogenesis
GO:0030146 diuresis
GO:0030147 natriuresis
GO:0030308 negative regulation of cell growth
GO:0042312 regulation of vasodilation
GO:0043114 regulation of vascular permeability
GO:0050880 regulation of blood vessel size
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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