Brain Natriuretic Peptide antibody | 50E1

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Mouse anti Human Brain Natriuretic Peptide

Product Type: Monoclonal Antibody
Clone: 50E1
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA2642	E IY WB			0.2 mg

Mouse anti Human Brain Natriuretic Peptide antibody, clone 50E1 detects an epitope located at the C-terminal (amino acids 26-32) of the human brain natriuretic peptide (BNP), a secreted protein which is a member of the natriuretic peptide family. BNP is a cardiac hormone, which is synthesised as a pro-hormone (proBNP), and is proteolytically cleaved to release a biologically active fragment (BNP), and an inactive fragment (NT-proBNP) into the circulation.

BNP is predominantly secreted from the cardiac ventricles in response to volume and pressure overload, and results in a number of biological activities including natriuresis, diuresis, vasorelaxation, and inhibition of the sympathetic nervous system. A high concentration of BNP in the bloodstream is indicative of heart failure.

Mouse anti Human Brain Natriuretic Peptide antibody, clone 50E1 also detects proBNP.

Product Details

Target Species

Human

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%

Sodium Azide (NaN₃)

Immunogen

Human synthetic Brain natriuretic hormone.

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

Fusion Partners

Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P16860 Related reagents
Entrez Gene: NPPB Related reagents
GO Terms: GO:0005615 extracellular space; GO:0006182 cGMP biosynthetic process; GO:0007168 receptor guanylyl cyclase signaling pathway; GO:0008217 regulation of blood pressure; GO:0008613 diuretic hormone activity; GO:0016525 negative regulation of angiogenesis; GO:0030146 diuresis; GO:0030147 natriuresis; GO:0030308 negative regulation of cell growth; GO:0042312 regulation of vasodilation; GO:0043114 regulation of vascular permeability; GO:0050880 regulation of blood vessel size
Regulatory: For research purposes only

Applications of Brain Natriuretic Peptide antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
Immunoassay
Western Blotting

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Western Blotting: MCA2642 detects a band of approximately 4kDa when using synthetic BNP and a band of 15kDa when using recombinant proBNP.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Product Specific References

References for Brain Natriuretic Peptide antibody

Seferian, K.R. et al. (2007) The brain natriuretic peptide (BNP) precursor is the major immunoreactive form of BNP in patients with heart failure.
Clin Chem. 53 (5): 866-73.
Tamm, N.N. et al. (2008) Novel immunoassay for quantification of brain natriuretic peptide and its precursor in human blood.
Clin Chem. 54 (9): 1511-8.