Brain Natriuretic Peptide antibody | 50E1
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Mouse anti Human Brain Natriuretic Peptide
- Product Type
- Monoclonal Antibody
- Clone
- 50E1
- Isotype
- IgG1
- Specificity
- Brain Natriuretic Peptide
Antibody Quality and 
Unrivaled 
| Mouse anti Human Brain Natriuretic Peptide antibody, clone 50E1 detects an epitope located at the C-terminal (amino acids 26-32) of the human brain natriuretic peptide (BNP), a secreted protein which is a member of the natriuretic peptide family. BNP is a cardiac hormone, which is synthesised as a pro-hormone (proBNP), and is proteolytically cleaved to release a biologically active fragment (BNP), and an inactive fragment (NT-proBNP) into the circulation. BNP is predominantly secreted from the cardiac ventricles in response to volume and pressure overload, and results in a number of biological activities including natriuresis, diuresis, vasorelaxation, and inhibition of the sympathetic nervous system. A high concentration of BNP in the bloodstream is indicative of heart failure. Mouse anti Human Brain Natriuretic Peptide antibody, clone 50E1 also detects proBNP. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Human synthetic Brain natriuretic hormone.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Immunoassay | |
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| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- MCA2642 detects a band of approximately 4kDa when using synthetic BNP and a band of 15kDa when using recombinant proBNP.
References for Brain Natriuretic Peptide antibody
-
Seferian, K.R. et al. (2007) The brain natriuretic peptide (BNP) precursor is the major immunoreactive form of BNP in patients with heart failure.
Clin Chem. 53 (5): 866-73. -
Tamm, N.N. et al. (2008) Novel immunoassay for quantification of brain natriuretic peptide and its precursor in human blood.
Clin Chem. 54 (9): 1511-8.
Further Reading
-
Clerico, A. et al. (2006) Clinical relevance of biological variation: the lesson of brain natriuretic peptide (BNP) and NT-proBNP assay.
Clin Chem Lab Med. 44 (4): 366-78. -
Pfister, R. & Schneider, C.A. (2004) Natriuretic peptides BNP and NT-pro-BNP: established laboratory markers in clinical practice or just perspectives?
Clin Chim Acta. 349 (1-2): 25-38.
- RRID
- AB_2155429
- UniProt
- P16860
- Entrez Gene
- NPPB
- GO Terms
- GO:0005615 extracellular space
- GO:0006182 cGMP biosynthetic process
- GO:0007168 receptor guanylyl cyclase signaling pathway
- GO:0008217 regulation of blood pressure
- GO:0008613 diuretic hormone activity
- GO:0016525 negative regulation of angiogenesis
- GO:0030146 diuresis
- GO:0030147 natriuresis
- GO:0030308 negative regulation of cell growth
- View More GO Terms
- GO:0042312 regulation of vasodilation
- GO:0043114 regulation of vascular permeability
- GO:0050880 regulation of blood vessel size
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