BNP Prohormone antibody | 18H5

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Mouse anti Human BNP Prohormone (N-Terminal)

Product Type
Monoclonal Antibody
Clone
18H5
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2641 E IY WB 0.2 mg
Mouse anti Human BNP Prohormone (N-Terminal) antibody, clone 18H5 detects the amino-terminal fragment of pro-brain natriuretic peptide (amino acids 13-20), also known as NT-proBNP, a cleavage product during the proteolytic activation of BNP. NT-proBNP is a secreted protein which is a member of the natriuretic peptide family, which is predominantly secreted from the cardiac ventricles in response to volume and pressure overload.

NT-proBNP is more stable than BNP in serum, and has become a useful biomarker of heart failure.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Amino acids 13-27 of NT-proBNP
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells of the Sp2/0 myeloma cell line

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch

More Information

UniProt
P16860 Related reagents
Entrez Gene
NPPB Related reagents
GO Terms
GO:0005615 extracellular space
GO:0006182 cGMP biosynthetic process
GO:0007168 receptor guanylyl cyclase signaling pathway
GO:0008217 regulation of blood pressure
GO:0008613 diuretic hormone activity
GO:0016525 negative regulation of angiogenesis
GO:0030146 diuresis
GO:0030147 natriuresis
GO:0030308 negative regulation of cell growth
GO:0042312 regulation of vasodilation
GO:0043114 regulation of vascular permeability
GO:0050880 regulation of blood vessel size
Regulatory
For research purposes only

Applications of BNP Prohormone antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunoassay
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
MCA2641 detects a band of approximately 9kDa when using recombinant NT-proBNP

Product Specific References

Further Reading

  1. Clerico, A. et al. (2006) Clinical relevance of biological variation: the lesson of brain natriuretic peptide (BNP) and NT-proBNP assay.
    Clin Chem Lab Med. 44 (4): 366-78.
  2. Pfister, R. & Schneider, C.A. (2004) Natriuretic peptides BNP and NT-pro-BNP: established laboratory markers in clinical practice or just perspectives?
    Clin Chim Acta. 349 (1-2): 25-38.