BNP Prohormone antibody | 18H5
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Mouse anti Human BNP Prohormone (N-Terminal)
- Product Type
- Monoclonal Antibody
- Clone
- 18H5
- Isotype
- IgG1
- Specificity
- BNP Prohormone
- Region
- (N-TERMINAL)
Antibody Quality and Performance Guarantee
| Mouse anti Human BNP Prohormone (N-Terminal) antibody, clone 18H5 detects the amino-terminal fragment of pro-brain natriuretic peptide (amino acids 13-20), also known as NT-proBNP, a cleavage product during the proteolytic activation of BNP. NT-proBNP is a secreted protein which is a member of the natriuretic peptide family, which is predominantly secreted from the cardiac ventricles in response to volume and pressure overload. NT-proBNP is more stable than BNP in serum, and has become a useful biomarker of heart failure. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Amino acids 13-27 of NT-proBNP
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells of the Sp2/0 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
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| Immunoassay | |
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| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- MCA2641 detects a band of approximately 9kDa when using recombinant NT-proBNP
Further Reading
-
Clerico, A. et al. (2006) Clinical relevance of biological variation: the lesson of brain natriuretic peptide (BNP) and NT-proBNP assay.
Clin Chem Lab Med. 44 (4): 366-78. -
Pfister, R. & Schneider, C.A. (2004) Natriuretic peptides BNP and NT-pro-BNP: established laboratory markers in clinical practice or just perspectives?
Clin Chim Acta. 349 (1-2): 25-38.
- Synonyms
- NT-proBNP
- RRID
- AB_2236317
- UniProt
- P16860
- Entrez Gene
- NPPB
- GO Terms
- GO:0005615 extracellular space
- GO:0006182 cGMP biosynthetic process
- GO:0007168 receptor guanylyl cyclase signaling pathway
- GO:0008217 regulation of blood pressure
- GO:0008613 diuretic hormone activity
- GO:0016525 negative regulation of angiogenesis
- GO:0030146 diuresis
- GO:0030147 natriuresis
- GO:0030308 negative regulation of cell growth
- View More GO Terms
- GO:0042312 regulation of vasodilation
- GO:0043114 regulation of vascular permeability
- GO:0050880 regulation of blood vessel size
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