Beta 2 Microglobulin antibody | B2M-01
Mouse anti Human beta 2 Microglobulin antibody, clone B2M-01 recognizes native beta 2 microglobulin which is a component of MHC class I molecules, which are present on all nucleated cells. Levels of beta 2 microglobulin can be used to assess renal function in kidney transplant recipients. Also known as B2M, it has been suggested that this protein can be used as a prognostic marker for HIV infection.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Dog Rabbit Chicken Bovine - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Product Form
- Ig fraction - liquid
- Preparation
- Purified IgG prepared from ascites using sequential precipitation using caprylic acid and ammonium sulphate.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
HRP Stabiliser (BUF052A) - Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Immunogen
- Beta-2 microglobulin from human urine.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- Ig concentration 1.0mg/ml
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P61769
- Entrez Gene
- B2M
- GO Terms
- GO:0000139 Golgi membrane
- GO:0005515 protein binding
- GO:0002474 antigen processing and presentation of peptide antigen via MHC class I
- GO:0042612 MHC class I protein complex
- GO:0006955 immune response
- GO:0016032 viral reproduction
- GO:0031901 early endosome membrane
- GO:0050690 regulation of defense response to virus by virus
- GO:0060333 interferon-gamma-mediated signaling pathway
- Regulatory
- For research purposes only
Applications of Beta 2 Microglobulin antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/250 | 1/2500 | |
| Immunohistology - Paraffin | |||
| Western Blotting | |||
| ELISA | |||
| Flow Cytometry | |||
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
|
Product Specific References
References for Beta 2 Microglobulin antibody
-
Hilgert, I. et al. (1984) The Use of Murine Monoclonal Antibody B2M-01 for Detection and Purification of Human beta 2- microglobulin.
Folia Biologica (Praha) 30: 369-376.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
