Beta 2 Microglobulin antibody | B2M-01

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Mouse anti Human Beta 2 Microglobulin:HRP

Mouse anti Human Beta 2 Microglobulin

Product Type
Monoclonal Antibody
Clone
B2M-01
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
6240-0354P E P 0.1 mg
6240-0354 E F 0.25 mg
Mouse anti Human beta 2 Microglobulin antibody, clone B2M-01 recognizes native beta 2 microglobulin which is a component of MHC class I molecules, which are present on all nucleated cells. Levels of beta 2 microglobulin can be used to assess renal function in kidney transplant recipients. Also known as B2M, it has been suggested that this protein can be used as a prognostic marker for HIV infection.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Dog
Rabbit
Chicken
Bovine
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Ig fraction - liquid
Preparation
Purified IgG prepared from ascites using sequential precipitation using caprylic acid and ammonium sulphate.
Preparation
Ig fraction prepared from ascites by ammonium sulphate and caprylic acid precipitation
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
HRP Stabiliser (BUF052A)
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Beta-2 microglobulin from human urine
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
Ig concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P61769 Related reagents
Entrez Gene
B2M Related reagents
GO Terms
GO:0000139 Golgi membrane
GO:0005515 protein binding
GO:0002474 antigen processing and presentation of peptide antigen via MHC class I
GO:0042612 MHC class I protein complex
GO:0006955 immune response
GO:0016032 viral reproduction
GO:0031901 early endosome membrane
GO:0050690 regulation of defense response to virus by virus
GO:0060333 interferon-gamma-mediated signaling pathway
Regulatory
For research purposes only

Applications of Beta 2 Microglobulin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/250 1/2500
Immunohistology - Paraffin
Western Blotting
ELISA
Flow Cytometry
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG2a Negative Control MCA929 100 Tests F

Product Specific References

References for Beta 2 Microglobulin antibody

  1. Hilgert, I. et al. (1984) The Use of Murine Monoclonal Antibody B2M-01 for Detection and Purification of Human beta 2- microglobulin.
    Folia Biologica (Praha) 30: 369-376.