Beta-2 Adrenergic Receptor antibody | R11E1

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Anti Human Beta-2 Adrenergic Receptor Antibody, clone R11E1 gallery image 1 — LPS stimulated U937 cells stained with Mouse anti Human beta-2 adrenergic receptor (MCA2784)

Mouse anti Human Beta-2 Adrenergic Receptor

Product Type: Monoclonal Antibody
Clone: R11E1
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA2784GA	E F IF IP			0.1 mg
MCA2784	E F IF IP			0.2 mg

Mouse anti Human beta-2 Adrenergic Receptor antibody, clone R11E1 recognizes human beta 2 adrenergic receptor (beta2AR), a member of the G protein-coupled receptor superfamily. Beta1 and Beta2 adrenergic receptors are functionally similar but have different affinities for the ligands adrenaline and noraderenaline. B2A receptors co-exist with the B1A receptors but one subtype usually predominates. B2A receptors have a higher affinity for adrenaline and mediate the relaxation of smooth muscle including bronchus, intestine & uterus. They are located presynaptically in the nerves, where they facilitate neurotransmitter release in the brain and regulate a variety of physiological processes. Ligand binding of the B2A receptor leads to the activation of adenylyl cyclase through interaction with the GTP-binding protein Gs.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse
Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Carrier Free

Yes

Immunogen

Purified human Beta-2 adrenergic receptor.

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

Fusion Partners

Cells from immunised Balb/c mice were fused with cells of the X63Ag8.653 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P07550 Related reagents
Entrez Gene: ADRB2 Related reagents
Regulatory: For research purposes only

Applications of Beta-2 Adrenergic Receptor antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA
Flow Cytometry	Neat	1/25
Immunofluorescence
Immunoprecipitation

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.
MCA2784 performs best when used with a biotinylated secondary anti mouse IgG antibody such as STAR11B, followed by Streptavidin:FITC (STAR2B).

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse IgG1 Negative Control	MCA928	100 Tests	F