Beta-2 Adrenergic Receptor antibody | R11E1
Mouse anti Human beta-2 Adrenergic Receptor antibody, clone R11E1 recognizes human beta 2 adrenergic receptor (beta2AR), a member of the G protein-coupled receptor superfamily. Beta1 and Beta2 adrenergic receptors are functionally similar but have different affinities for the ligands adrenaline and noraderenaline. B2A receptors co-exist with the B1A receptors but one subtype usually predominates. B2A receptors have a higher affinity for adrenaline and mediate the relaxation of smooth muscle including bronchus, intestine & uterus. They are located presynaptically in the nerves, where they facilitate neurotransmitter release in the brain and regulate a variety of physiological processes. Ligand binding of the B2A receptor leads to the activation of adenylyl cyclase through interaction with the GTP-binding protein Gs.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Purified human Beta-2 adrenergic receptor.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Cells from immunised Balb/c mice were fused with cells of the X63Ag8.653 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
Applications of Beta-2 Adrenergic Receptor antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Flow Cytometry | Neat | 1/25 | |
| Immunofluorescence | |||
| Immunohistology - Paraffin | |||
| Immunoprecipitation |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Product Specific References
Further Reading
-
Frielle, T. et al. (1989) Properties of the beta 1- and beta 2-adrenergic receptor subtypes revealed by molecular cloning.
Clin Chem. 35 (5): 721-5.
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