Aquaporin 1 antibody | 1/A5F6

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Mouse anti Aquaporin 1

Product Type
Monoclonal Antibody
Clone
1/A5F6
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2099 C E IF P* WB 0.1 mg
Mouse anti Human aquaporin 1 antibody, clone 1/A5F6 recognizes an epitope within the cytoplasmic domain of the water-specific channel aquaporin 1, also known as AQP1 or CHIP-28.

Aquaporin 1 is a ~28 kDa integral membrane protein which was originally identified in red blood cells and the kidney. AQP1 is also expressed by the choroid plexus and various other tissues. The glycosylated forms of AQP1 range between 40-60 kDa.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
Rabbit
Zebrafish
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
0.1% Bovine Serum Albumin
Immunogen
Synthetic peptide corresponding to amino acids 249-269 of aquaporin 1.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with the cells of the Sp-2/0 Ag14 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P29972 Related reagents
Entrez Gene
AQP1 Related reagents
GO Terms
GO:0005515 protein binding
GO:0003097 renal water transport
GO:0005887 integral to plasma membrane
GO:0005223 intracellular cGMP activated cation channel activity
GO:0005267 potassium channel activity
GO:0005737 cytoplasm
GO:0006182 cGMP biosynthetic process
GO:0006884 cell volume homeostasis
GO:0046878 positive regulation of saliva secretion
GO:0042493 response to drug
GO:0009925 basal plasma membrane
GO:0015079 potassium ion transmembrane transporter activity
GO:0015168 glycerol transmembrane transporter activity
GO:0015250 water channel activity
GO:0015696 ammonium transport
GO:0016324 apical plasma membrane
GO:0020003 symbiont-containing vacuole
GO:0021670 lateral ventricle development
GO:0030157 pancreatic juice secretion
GO:0030184 nitric oxide transmembrane transporter activity
GO:0030950 establishment or maintenance of actin cytoskeleton polarity
GO:0031526 brush border membrane
GO:0031965 nuclear membrane
GO:0033326 cerebrospinal fluid secretion
GO:0034644 cellular response to UV
GO:0035377 transepithelial water transport
GO:0035379 carbon dioxide transmembrane transporter activity
GO:0042383 sarcolemma
GO:0042476 odontogenesis
GO:0043066 negative regulation of apoptosis
GO:0045766 positive regulation of angiogenesis
GO:0048146 positive regulation of fibroblast proliferation
GO:0051739 ammonia transmembrane transporter activity
GO:0070301 cellular response to hydrogen peroxide
GO:0071260 cellular response to mechanical stimulus
GO:0071280 cellular response to copper ion
GO:0071288 cellular response to mercury ion
GO:0071300 cellular response to retinoic acid
GO:0071320 cellular response to cAMP
GO:0071456 cellular response to hypoxia
GO:0071472 cellular response to salt stress
GO:0071474 cellular hyperosmotic response
GO:0071549 cellular response to dexamethasone stimulus
GO:0071732 cellular response to nitric oxide
GO:0085018 maintenance of symbiont-containing vacuole via substance secreted by host
Regulatory
For research purposes only

Applications of Aquaporin 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1000 1/20000
Immunofluorescence
Immunohistology - Frozen 1/500 1/1000
Immunohistology - Paraffin 1 1/500 1/1000
Western Blotting 1/1000 1/5000
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Histology Positive Control Tissue
Kidney

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Application Based External Images

Flow Cytometry

Immunofluorescence

Product Specific References

References for Aquaporin 1 antibody

  1. Nagy G et al. (2002) Development and characterisation of a monoclonal antibody family against aquaporin 1 (AQP1) and aquaporin 4 (AQP4).
    Pathol Oncol Res. 8 (2): 115-24.
  2. Bódis, B. et al. (2001) Active water selective channels in the stomach: investigation of aquaporins after ethanol and capsaicin treatment in rats.
    J Physiol Paris. 95 (1-6): 271-5.
  3. Francone, V.P. et al (2010) Signaling from the secretory granule to the nucleus: Uhmk1 and PAM.
    Mol Endocrinol. 24: 1543-58.
  4. Felszeghy, S. et al (2004) Expression of aquaporin isoforms during human and mouse tooth development.
    Arch Oral Biol. 49: 247-57.
  5. Ripoche, P. et al. (2004) Human Rhesus-associated glycoprotein mediates facilitated transport of NH(3) into red blood cells.
    Proc Natl Acad Sci U S A. 101: 17222-7.
  6. Lupp, A. et al. (2010) Immunohistochemical identification of the PTHR1 parathyroid hormone receptor in normal and neoplastic human tissues.
    Eur J Endocrinol. 162: 979-86.
  7. Pannabecker, T.L. et al. (2008) Quantitative analysis of functional reconstructions reveals lateral and axial zonation in the renal inner medulla.
    Am J Physiol Renal Physiol. 294: F1306-14.
  8. Thiele, S. et al. (2007) A disruptive mutation in exon 3 of the GNAS gene with albright hereditary osteodystrophy, normocalcemic pseudohypoparathyroidism, and selective long transcript variant Gsalpha-L deficiency.
    J Clin Endocrinol Metab. 92: 1764-8.
  9. Antonelou, M.H. et al. (2011) Oxidative stress-associated shape transformation and membrane proteome remodeling in erythrocytes of end stage renal disease patients on hemodialysis.
    J Proteomics. 74: 2441-52.
  10. Arnaud, L. et al. (2010) A functional AQP1 allele producing a Co(a-b-) phenotype revises and extends the Colton blood group system.
    Transfusion. 50: 2106-16.
  11. Saison, C. et al. (2012) A new AQP1 null allele identified in a Gypsy woman who developed an anti-CO3 during her first pregnancy.
    Vox Sang. 103: 137-44.