Aggrecan antibody | 7D4

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Anti Human Aggrecan Antibody, clone 7D4 thumbnail image 1

Anti Human Aggrecan Antibody, clone 7D4 thumbnail image 2

Anti Human Aggrecan Antibody, clone 7D4 gallery image 1 — Recombinant human aggrecan detected with Mouse anti Human Aggrecan (**MCA1454G**) followed by Goat anti Mouse IgG:Dylight^®800. Signal was visualized using a LI-COR Odyssey^® infrared imaging system.

Anti Human Aggrecan Antibody, clone 7D4 gallery image 2 — **Published customer image:**
Mouse anti Human aggrecan antibody, clone 7D4 (**MCA1454**) used for the detection of aggrecan in bovine synovial explants by immunohistochemistry on formalin fixed, paraffin embedded tissue sections
**Image caption:**
Influence of TGF-β1 on the BMP-2-induced deposition of cartilaginous proteins in synovial explants (immunohistochemical analysis). Light micrographs of sections through bovine explants that had been cultured for 6 weeks under the indicated stimulation conditions and then either stained with Toluidine Blue (1st row), or treated for the immunohistochemical detection of aggrecan (2nd row), type-II collagen (3rd row), type-IX collagen (4th row), type-X collagen (5th row) or COMP (6th row). BMP-2 was applied at a concentration of 200 ng/ml, and TGF-β1 at one of 10 ng/ml. Each agent was introduced at the onset of the 6-week culturing period and was present throughout its course. Native (uncultured) metacarpal-joint cartilage (final vertical column) served as a positive control. Cell nuclei (2nd to 6th rows) were counterstained with haematoxylin. All photomicrographs are represented at the same final magnification. Bar = 100 μm.

From: Shintani N, Siebenrock KA, Hunziker EB (2013)
TGF-β1 Enhances the BMP-2-Induced Chondrogenesis of Bovine Synovial Explants and Arrests Downstream Differentiation at an Early Stage of Hypertrophy.
.PLoS ONE 8(1): e53086.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Human Aggrecan

Product Type: Monoclonal Antibody
Clone: 7D4
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA1454G	C E IP P WB			0.2 mg

Mouse anti Human Aggrecan antibody, clone 7D4 recognizes human aggrecan, a proteoglycan and member of the aggrecan/versican proteoglycan family, which forms a major component of the extracellular matrix (ECM) of both cartilage and the central nervous system (CNS).

Studies using a panel of core protein-directed antibodies against human aggrecan, revealed the distribution of different aggrecan isoforms within the CNS, and sub-divided the isoforms into clusters 1-5 accordingly. These studies also showed a difference in the relative abundance of these isoforms when comparing brain and cartilage tissues (Virgintino et al. 2009).

Mouse anti Human Aggrecan antibody, clone 7D4 recognizes epitopes within the N-terminal G1-IGD-G2 region of aggrecan, that has been identified as a cluster 1 isoform.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Bovine
Chicken
Fish
Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Carrier Free

Yes

Immunogen

Purified human articular cartilage aggrecan

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

Fusion Partners

Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P16112 Related reagents
Entrez Gene: ACAN Related reagents
GO Terms: GO:0005515 protein binding; GO:0001501 skeletal system development; GO:0007155 cell adhesion; GO:0005201 extracellular matrix structural constituent; GO:0005529 sugar binding; GO:0005540 hyaluronic acid binding; GO:0005578 proteinaceous extracellular matrix; GO:0006508 proteolysis
Regulatory: For research purposes only

Applications of Aggrecan antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Histology Positive Control Tissue: Cerebral Cortex

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Product Specific References

References for Aggrecan antibody

Virgintino, D. et al. (2009) Differential distribution of aggrecan isoforms in perineuronal nets of the human cerebral cortex.
J Cell Mol Med. 13 (9B): 3151-73.
Shintani, N. and Hunziker, E.B. (2011) Differential effects of dexamethasone on the chondrogenesis of mesenchymal stromal cells: Influence of microenvironment, tissue origin and growth factor.
Eur Cell Mater. 22: 302-20.
Gaál B et al. (2014) Distribution of extracellular matrix macromolecules in the vestibular nuclei and cerebellum of the frog, Rana esculenta.
Neuroscience. 258: 162-73.
Suttkus, A. et al. (2014) Aggrecan, link protein and tenascin-R are essential components of the perineuronal net to protect neurons against iron-induced oxidative stress.
Cell Death Dis. 5: e1119.
Betre, H. et al. (2002) A two-step chondrocyte recovery system based on thermally sensitive elastin-like polypeptide scaffolds for cartilage tissue engineering
Engineering in Medicine and Biology 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society EMBS/BMES Conference,: 829-30.
Shintani N et al. (2013) TGF-β1 enhances the BMP-2-induced chondrogenesis of bovine synovial explants and arrests downstream differentiation at an early stage of hypertrophy.
PLoS One. 8 (1): e53086.
Murakami, T. & Ohtsuka, A. (2003) Perisynaptic barrier of proteoglycans in the mature brain and spinal cord.
Arch Histol Cytol. 66 (3): 195-207.
Wan, S. et al. (2016) Self-assembling peptide hydrogel for intervertebral disc tissue engineering.
Acta Biomater. pii: S1742-7061(16)30506-2. [Epub ahead of print]
Blosa, M. et al. (2013) Unique features of extracellular matrix in the mouse medial nucleus of trapezoid body--implications for physiological functions.
Neuroscience. 228: 215-34.
Goldberg-Bockhorn, E. et al. (2017) Laser surface modification of decellularized extracellular cartilage matrix for cartilage tissue engineering.
Lasers Med Sci. Dec 06 [Epub ahead of print].
Rogers, S.L. et al. (2018) Normal development of the Perineuronal net in humans; in patients with and without epilepsy.
Neuroscience. Jun 06 [Epub ahead of print].

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