Aggrecan antibody | 7D4

Mouse anti Human Aggrecan

Product Type
Monoclonal Antibody
Clone
7D4
Isotype
IgG1
Specificity
Aggrecan

Product Code Applications Pack Size List Price Your Price Qty
MCA1454G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E IP P WB 0.2 mg loader
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loader
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Mouse anti Human Aggrecan antibody, clone 7D4 recognizes human aggrecan, a proteoglycan and member of the aggrecan/versican proteoglycan family, which forms a major component of the extracellular matrix (ECM) of both cartilage and the central nervous system (CNS).

Using a panel of core protein-directed antibodies directed against human aggrecan, revealed the distribution of different aggrecan isoforms within the CNS, and sub-divided the isoforms into clusters 1-5 accordingly. highlighting a difference in the relative abundance of these isoforms when comparing brain and cartilage tissues (Virgintino et al. 2009).  

Mouse anti Human Aggrecan antibody, clone 7D4 recognizes epitopes within the N-terminal G1-IGD-G2 region of aggrecan, identified as a cluster 1 isoform.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine
Chicken
Fish
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Purified human articular cartilage aggrecan
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Cerebral Cortex

Description Product Code Applications Pack Size List Price Your Price Quantity
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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
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Description Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
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Description Goat anti Mouse IgG (Fc):HRP
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

References for Aggrecan antibody

  1. Murakami, T. & Ohtsuka, A. (2003) Perisynaptic barrier of proteoglycans in the mature brain and spinal cord.
    Arch Histol Cytol. 66 (3): 195-207.
  2. Virgintino, D. et al. (2009) Differential distribution of aggrecan isoforms in perineuronal nets of the human cerebral cortex.
    J Cell Mol Med. 13 (9B): 3151-73.
  3. Shintani, N. and Hunziker, E.B. (2011) Differential effects of dexamethasone on the chondrogenesis of mesenchymal stromal cells: Influence of microenvironment, tissue origin and growth factor.
    Eur Cell Mater. 22: 302-20.
  4. Gaál B et al. (2014) Distribution of extracellular matrix macromolecules in the vestibular nuclei and cerebellum of the frog, Rana esculenta.
    Neuroscience. 258: 162-73.
  5. Suttkus, A. et al. (2014) Aggrecan, link protein and tenascin-R are essential components of the perineuronal net to protect neurons against iron-induced oxidative stress.
    Cell Death Dis. 5: e1119.
  6. Betre, H. et al. (2002) A two-step chondrocyte recovery system based on thermally sensitive elastin-like polypeptide scaffolds for cartilage tissue engineering
    Engineering in Medicine and Biology 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society EMBS/BMES Conference,: 829-30.
  7. Shintani N et al. (2013) TGF-β1 enhances the BMP-2-induced chondrogenesis of bovine synovial explants and arrests downstream differentiation at an early stage of hypertrophy.
    PLoS One. 8 (1): e53086.
  8. Blosa, M. et al. (2013) Unique features of extracellular matrix in the mouse medial nucleus of trapezoid body--implications for physiological functions.
    Neuroscience. 228: 215-34.
  9. View The Latest Product References
  10. Wan, S. et al. (2016) Self-assembling peptide hydrogel for intervertebral disc tissue engineering.
    Acta Biomater. 46: 29-40.
  11. Rogers, S.L. et al. (2018) Normal Development of the Perineuronal Net in Humans; In Patients with and without Epilepsy.
    Neuroscience. 384: 350-60.
  12. Goldberg-Bockhorn, E. et al. (2018) Laser surface modification of decellularized extracellular cartilage matrix for cartilage tissue engineering.
    Lasers Med Sci. 33 (2): 375-84.
  13. De Moor, L. et al. (2020) Scaffold Free Microtissue Formation for Enhanced Cartilage Repair.
    Ann Biomed Eng. 48 (1): 298-311.

RRID
AB_10961289
UniProt
P16112
Entrez Gene
ACAN
GO Terms
GO:0005515 protein binding
GO:0001501 skeletal system development
GO:0007155 cell adhesion
GO:0005201 extracellular matrix structural constituent
GO:0005529 sugar binding
GO:0005540 hyaluronic acid binding
GO:0005578 proteinaceous extracellular matrix
GO:0006508 proteolysis
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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