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Aggrecan antibody | 7D4

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Anti Human Aggrecan Antibody, clone 7D4 thumbnail image 1 Anti Human Aggrecan Antibody, clone 7D4 thumbnail image 2
Anti Human Aggrecan Antibody, clone 7D4 gallery image 1
Recombinant human aggrecan detected with Mouse anti Human Aggrecan (MCA1454G) followed by Goat anti Mouse IgG:Dylight®800. Signal was visualized using a LI-COR Odyssey® infrared imaging system.
Anti Human Aggrecan Antibody, clone 7D4 gallery image 2
Published customer image:
Mouse anti Human aggrecan antibody, clone 7D4 (MCA1454) used for the detection of aggrecan in bovine synovial explants by immunohistochemistry on formalin fixed, paraffin embedded tissue sections
Image caption:
Influence of TGF-β1 on the BMP-2-induced deposition of cartilaginous proteins in synovial explants (immunohistochemical analysis). Light micrographs of sections through bovine explants that had been cultured for 6 weeks under the indicated stimulation conditions and then either stained with Toluidine Blue (1st row), or treated for the immunohistochemical detection of aggrecan (2nd row), type-II collagen (3rd row), type-IX collagen (4th row), type-X collagen (5th row) or COMP (6th row). BMP-2 was applied at a concentration of 200 ng/ml, and TGF-β1 at one of 10 ng/ml. Each agent was introduced at the onset of the 6-week culturing period and was present throughout its course. Native (uncultured) metacarpal-joint cartilage (final vertical column) served as a positive control. Cell nuclei (2nd to 6th rows) were counterstained with haematoxylin. All photomicrographs are represented at the same final magnification. Bar = 100 μm.

From: Shintani N, Siebenrock KA, Hunziker EB (2013)
TGF-β1 Enhances the BMP-2-Induced Chondrogenesis of Bovine Synovial Explants and Arrests Downstream Differentiation at an Early Stage of Hypertrophy.
.PLoS ONE 8(1): e53086.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Human Aggrecan

Product Type
Monoclonal Antibody
Clone
7D4
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA1454G
Datasheet
C E IP P WB
MSDS
0.2 mg loader

Mouse anti Human Aggrecan antibody, clone 7D4 recognizes human aggrecan, a proteoglycan and member of the aggrecan/versican proteoglycan family, which forms a major component of the extracellular matrix (ECM) of both cartilage and the central nervous system (CNS).

Studies using a panel of core protein-directed antibodies against human aggrecan, revealed the distribution of different aggrecan isoforms within the CNS, and sub-divided the isoforms into clusters 1-5 accordingly. These studies also showed a difference in the relative abundance of these isoforms when comparing brain and cartilage tissues (Virgintino et al. 2009).  

Mouse anti Human Aggrecan antibody, clone 7D4 recognizes epitopes within the N-terminal G1-IGD-G2 region of aggrecan, that has been identified as a cluster 1 isoform.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine
Chicken
Fish
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Purified human articular cartilage aggrecan
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

UniProt
P16112 Related reagents
Entrez Gene
ACAN Related reagents
GO Terms
GO:0005515 protein binding
GO:0001501 skeletal system development
GO:0007155 cell adhesion
GO:0005201 extracellular matrix structural constituent
GO:0005529 sugar binding
GO:0005540 hyaluronic acid binding
GO:0005578 proteinaceous extracellular matrix
GO:0006508 proteolysis
Regulatory
For research purposes only

Applications of Aggrecan antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Cerebral Cortex
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Product Specific References

References for Aggrecan antibody

  1. Virgintino, D. et al. (2009) Differential distribution of aggrecan isoforms in perineuronal nets of the human cerebral cortex.
    J Cell Mol Med. 13 (9B): 3151-73.
  2. Shintani, N. and Hunziker, E.B. (2011) Differential effects of dexamethasone on the chondrogenesis of mesenchymal stromal cells: Influence of microenvironment, tissue origin and growth factor.
    Eur Cell Mater. 22: 302-20.
  3. Gaál B et al. (2014) Distribution of extracellular matrix macromolecules in the vestibular nuclei and cerebellum of the frog, Rana esculenta.
    Neuroscience. 258: 162-73.
  4. Suttkus, A. et al. (2014) Aggrecan, link protein and tenascin-R are essential components of the perineuronal net to protect neurons against iron-induced oxidative stress.
    Cell Death Dis. 5: e1119.
  5. Betre, H. et al. (2002) A two-step chondrocyte recovery system based on thermally sensitive elastin-like polypeptide scaffolds for cartilage tissue engineering
    Engineering in Medicine and Biology 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society EMBS/BMES Conference,: 829-30.
  6. Shintani N et al. (2013) TGF-β1 enhances the BMP-2-induced chondrogenesis of bovine synovial explants and arrests downstream differentiation at an early stage of hypertrophy.
    PLoS One. 8 (1): e53086.
  7. Murakami, T. & Ohtsuka, A. (2003) Perisynaptic barrier of proteoglycans in the mature brain and spinal cord.
    Arch Histol Cytol. 66 (3): 195-207.
  8. Wan, S. et al. (2016) Self-assembling peptide hydrogel for intervertebral disc tissue engineering.
    Acta Biomater. pii: S1742-7061(16)30506-2. [Epub ahead of print]
  9. Blosa, M. et al. (2013) Unique features of extracellular matrix in the mouse medial nucleus of trapezoid body--implications for physiological functions.
    Neuroscience. 228: 215-34.
  10. Goldberg-Bockhorn, E. et al. (2018) Laser surface modification of decellularized extracellular cartilage matrix for cartilage tissue engineering.
    Lasers Med Sci. 33 (2): 375-84.
  11. Rogers, S.L. et al. (2018) Normal Development of the Perineuronal Net in Humans; In Patients with and without Epilepsy.
    Neuroscience. 384: 350-60.
  12. De Moor, L. et al. (2019) Scaffold Free Microtissue Formation for Enhanced Cartilage Repair.
    Ann Biomed Eng. Aug 26 [Epub ahead of print].
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