Aggrecan antibody | 7D4
Mouse anti Human aggrecan antibody, clone 7D4 (MCA1454) used for the detection of aggrecan in bovine synovial explants by immunohistochemistry on formalin fixed, paraffin embedded tissue sections
Image caption:
Influence of TGF-β1 on the BMP-2-induced deposition of cartilaginous proteins in synovial explants (immunohistochemical analysis). Light micrographs of sections through bovine explants that had been cultured for 6 weeks under the indicated stimulation conditions and then either stained with Toluidine Blue (1st row), or treated for the immunohistochemical detection of aggrecan (2nd row), type-II collagen (3rd row), type-IX collagen (4th row), type-X collagen (5th row) or COMP (6th row). BMP-2 was applied at a concentration of 200 ng/ml, and TGF-β1 at one of 10 ng/ml. Each agent was introduced at the onset of the 6-week culturing period and was present throughout its course. Native (uncultured) metacarpal-joint cartilage (final vertical column) served as a positive control. Cell nuclei (2nd to 6th rows) were counterstained with haematoxylin. All photomicrographs are represented at the same final magnification. Bar = 100 μm.
From: Shintani N, Siebenrock KA, Hunziker EB (2013)
TGF-β1 Enhances the BMP-2-Induced Chondrogenesis of Bovine Synovial Explants and Arrests Downstream Differentiation at an Early Stage of Hypertrophy.
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This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Filter by Application:
WB P ResetMouse anti Human Aggrecan
- Product Type
- Monoclonal Antibody
- Clone
- 7D4
- Isotype
- IgG1
- Specificity
- Aggrecan
Antibody Quality and 
Unrivaled 
| Mouse anti Human Aggrecan antibody, clone 7D4 recognizes human aggrecan, a proteoglycan and member of the aggrecan/versican proteoglycan family, which forms a major component of the extracellular matrix (ECM) of both cartilage and the central nervous system (CNS). Using a panel of core protein-directed antibodies directed against human aggrecan, revealed the distribution of different aggrecan isoforms within the CNS, and sub-divided the isoforms into clusters 1-5 accordingly. highlighting a difference in the relative abundance of these isoforms when comparing brain and cartilage tissues (Virgintino et al. 2009). Mouse anti Human Aggrecan antibody, clone 7D4 recognizes epitopes within the N-terminal G1-IGD-G2 region of aggrecan, identified as a cluster 1 isoform. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bovine Chicken Fish Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Purified human articular cartilage aggrecan
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
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| Immunohistology - Frozen | |
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| Immunohistology - Paraffin | |
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| Immunoprecipitation | |
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| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Cerebral Cortex
References for Aggrecan antibody
-
Murakami, T. & Ohtsuka, A. (2003) Perisynaptic barrier of proteoglycans in the mature brain and spinal cord.
Arch Histol Cytol. 66 (3): 195-207. -
Virgintino, D. et al. (2009) Differential distribution of aggrecan isoforms in perineuronal nets of the human cerebral cortex.
J Cell Mol Med. 13 (9B): 3151-73. -
Shintani, N. and Hunziker, E.B. (2011) Differential effects of dexamethasone on the chondrogenesis of mesenchymal stromal cells: Influence of microenvironment, tissue origin and growth factor.
Eur Cell Mater. 22: 302-20. -
Gaál B et al. (2014) Distribution of extracellular matrix macromolecules in the vestibular nuclei and cerebellum of the frog, Rana esculenta.
Neuroscience. 258: 162-73. -
Suttkus, A. et al. (2014) Aggrecan, link protein and tenascin-R are essential components of the perineuronal net to protect neurons against iron-induced oxidative stress.
Cell Death Dis. 5: e1119. -
Betre, H. et al. (2002) A two-step chondrocyte recovery system based on thermally sensitive elastin-like polypeptide scaffolds for cartilage tissue engineering
Engineering in Medicine and Biology 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society EMBS/BMES Conference,: 829-30. -
Shintani N et al. (2013) TGF-β1 enhances the BMP-2-induced chondrogenesis of bovine synovial explants and arrests downstream differentiation at an early stage of hypertrophy.
PLoS One. 8 (1): e53086. -
Blosa, M. et al. (2013) Unique features of extracellular matrix in the mouse medial nucleus of trapezoid body--implications for physiological functions.
Neuroscience. 228: 215-34.
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Goldberg-Bockhorn, E. et al. (2018) Laser surface modification of decellularized extracellular cartilage matrix for cartilage tissue engineering.
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De Moor, L. et al. (2020) Scaffold Free Microtissue Formation for Enhanced Cartilage Repair.
Ann Biomed Eng. 48 (1): 298-311.
- RRID
- AB_10961289
- UniProt
- P16112
- Entrez Gene
- ACAN
- GO Terms
- GO:0005515 protein binding
- GO:0001501 skeletal system development
- GO:0007155 cell adhesion
- GO:0005201 extracellular matrix structural constituent
- GO:0005529 sugar binding
- GO:0005540 hyaluronic acid binding
- GO:0005578 proteinaceous extracellular matrix
- GO:0006508 proteolysis
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