Actin Alpha antibody | 1A4
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|Mouse anti human actin alpha (smooth muscle) antibody, clone 1A4 recognises alpha smooth muscle actin. Actins are highly conserved proteins that play a role in cell contractile activity, motility, cytokinesis, and in maintaining cell structure. Alpha smooth muscle actin is expressed in vascular smooth muscle, myofibroblasts and myoepithelial cells and also in tumours derived from these cells.|
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Pig Rabbit Rat Mouse Sheep
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antibody purified from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Acetylated N-terminal synthetic decapeptide of alpha smooth muscle actin coupled to KLH.
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen||1/50||1/100|
|Immunohistology - Paraffin 1|
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
- Histology Positive Control Tissue
- Smooth muscle in vessels
References for Actin Alpha antibody
Ando, H. et al. (1999) Cardiac microvascular endothelial cells express alpha-smooth muscle actin and show low NOS III activity.
Am J Physiol. 276 (5 Pt 2): H1755-68.
Rønnov-jessen, L. & Petersen, O.W. (1996) A function for filamentous alpha-smooth muscle actin: retardation of motility in fibroblasts.
J Cell Biol. 134 (1): 67-80.
Paranya G et al. (2001) Aortic valve endothelial cells undergo transforming growth factor-beta-mediated and non-transforming growth factor-beta-mediated transdifferentiation in vitro.
Am J Pathol. 159 (4): 1335-43.
Lavaud S et al. (2001) Inflammation is probably not a prerequisite for renal interstitial fibrosis in normoglycemic obese rats.
Am J Physiol Renal Physiol. 280 (4): F683-94.
Skalli, O. et al. (1986) A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation.
J Cell Biol. 103 (6 Pt 2): 2787-96.
Darby, I. et al. (1990) Alpha-smooth muscle actin is transiently expressed by myofibroblasts during experimental wound healing.
Lab Invest. 63: 21-29
Desmoulière, A. et al. (1993) Transforming growth factor-beta 1 induces alpha-smooth muscle actin expression in granulation tissue myofibroblasts and in quiescent and growing cultured fibroblasts.
J Cell Biol. 122 (1): 103-11.
Hinz, B. et al. (2001) Alpha-smooth muscle actin expression upregulates fibroblast contractile activity.
Mol Biol Cell. 12 (9): 2730-41.
View The Latest Product References
Hinz, B. et al. (2002) The NH2-terminal peptide of alpha-smooth muscle actin inhibits force generation by the myofibroblast in vitro and in vivo.
J Cell Biol. 157 (4): 657-63.
Shin, M.O. et al. (2010) Effect of dietary supplementation of grape skin and seeds on liver fibrosis induced by dimethylnitrosamine in rats.
Nutr Res Pract. 4: 369-74.
Brisset, A.C. et al. (2007) Intimal smooth muscle cells of porcine and human coronary artery express S100A4, a marker of the rhomboid phenotype in vitro.
Circ Res. 100: 1055-62.
Chaponnier, C. & Gabbiani, G. (2016) Monoclonal antibodies against muscle actin isoforms: epitope identification and analysis of isoform expression by immunoblot and immunostaining in normal and regenerating skeletal muscle.
F1000Res. 5: 416.
Stammler, A. et al. (2015) Epididymitis: ascending infection restricted by segmental boundaries.
Hum Reprod. 30 (7): 1557-65.
Sievers, H. et al. (2020) Pododermal angioarchitecture in the equine hoof wall: A light and scanning electron microscopic study of the wall proper.
Clin Hemorheol Microcirc. 74 (1): 21-44.
Zheng, Z. et al. (2022) In Vivo. Inhibition of TRPC6 by SH045 Attenuates Renal Fibrosis in a New Zealand Obese (NZO) Mouse Model of Metabolic Syndrome.
Int J Mol Sci. 23 (12): 6870.
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