Actin Alpha antibody | 1A4

Mouse anti Human Actin Alpha (Smooth Muscle)

Product Type
Monoclonal Antibody
Clone
1A4
Isotype
IgG2a
Specificity
Actin Alpha
Region
(SMOOTH MUSCLE)

Product Code Applications Pack Size List Price Your Price Qty
MCA5781GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E IF P * WB 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti human actin alpha (smooth muscle) antibody, clone 1A4 recognises alpha smooth muscle actin. Actins are highly conserved proteins that play a role in cell contractile activity, motility, cytokinesis, and in maintaining cell structure. Alpha smooth muscle actin is expressed in vascular smooth muscle, myofibroblasts and myoepithelial cells and also in tumours derived from these cells.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Pig
Rabbit
Rat
Mouse
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Antibody purified from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Acetylated N-terminal synthetic decapeptide of alpha smooth muscle actin coupled to KLH.
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunofluorescence
Immunohistology - Frozen 1/50 1/100
Immunohistology - Paraffin 1
Western Blotting
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Smooth muscle in vessels

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References for Actin Alpha antibody

  1. Ando, H. et al. (1999) Cardiac microvascular endothelial cells express alpha-smooth muscle actin and show low NOS III activity.
    Am J Physiol. 276 (5 Pt 2): H1755-68.
  2. Rønnov-jessen, L. & Petersen, O.W. (1996) A function for filamentous alpha-smooth muscle actin: retardation of motility in fibroblasts.
    J Cell Biol. 134 (1): 67-80.
  3. Paranya G et al. (2001) Aortic valve endothelial cells undergo transforming growth factor-beta-mediated and non-transforming growth factor-beta-mediated transdifferentiation in vitro.
    Am J Pathol. 159 (4): 1335-43.
  4. Lavaud S et al. (2001) Inflammation is probably not a prerequisite for renal interstitial fibrosis in normoglycemic obese rats.
    Am J Physiol Renal Physiol. 280 (4): F683-94.
  5. Skalli, O. et al. (1986) A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation.
    J Cell Biol. 103 (6 Pt 2): 2787-96.
  6. Darby, I. et al. (1990) Alpha-smooth muscle actin is transiently expressed by myofibroblasts during experimental wound healing.
    Lab Invest. 63: 21-29
  7. Desmoulière, A. et al. (1993) Transforming growth factor-beta 1 induces alpha-smooth muscle actin expression in granulation tissue myofibroblasts and in quiescent and growing cultured fibroblasts.
    J Cell Biol. 122 (1): 103-11.
  8. Hinz, B. et al. (2001) Alpha-smooth muscle actin expression upregulates fibroblast contractile activity.
    Mol Biol Cell. 12 (9): 2730-41.
  9. View The Latest Product References
  10. Hinz, B. et al. (2002) The NH2-terminal peptide of alpha-smooth muscle actin inhibits force generation by the myofibroblast in vitro and in vivo.
    J Cell Biol. 157 (4): 657-63.
  11. Shin, M.O. et al. (2010) Effect of dietary supplementation of grape skin and seeds on liver fibrosis induced by dimethylnitrosamine in rats.
    Nutr Res Pract. 4: 369-74.
  12. Brisset, A.C. et al. (2007) Intimal smooth muscle cells of porcine and human coronary artery express S100A4, a marker of the rhomboid phenotype in vitro.
    Circ Res. 100: 1055-62.
  13. Chaponnier, C. & Gabbiani, G. (2016) Monoclonal antibodies against muscle actin isoforms: epitope identification and analysis of isoform expression by immunoblot and immunostaining in normal and regenerating skeletal muscle.
    F1000Res. 5: 416.
  14. Stammler, A. et al. (2015) Epididymitis: ascending infection restricted by segmental boundaries.
    Hum Reprod. 30 (7): 1557-65.
  15. Sievers, H. et al. (2020) Pododermal angioarchitecture in the equine hoof wall: A light and scanning electron microscopic study of the wall proper.
    Clin Hemorheol Microcirc. 74 (1): 21-44.
  16. Zheng, Z. et al. (2022) In Vivo. Inhibition of TRPC6 by SH045 Attenuates Renal Fibrosis in a New Zealand Obese (NZO) Mouse Model of Metabolic Syndrome.
    Int J Mol Sci. 23 (12): 6870.

Immunohistology - Paraffin

UniProt
P62736
Entrez Gene
ACTA2
GO Terms
GO:0009615 response to virus
GO:0005829 cytosol
GO:0005524 ATP binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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