Actin Alpha antibody | 1A4
Mouse anti human actin alpha (smooth muscle) antibody, clone 1A4 recognises alpha smooth muscle actin. Actins are highly conserved proteins that play a role in cell contractile activity, motility, cytokinesis, and in maintaining cell structure. Alpha smooth muscle actin is expressed in vascular smooth muscle, myofibroblasts and myoepithelial cells and also in tumours derived from these cells.
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Pig Rabbit Rat Mouse Sheep
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Acetylated N-terminal synthetic decapeptide of alpha smooth muscle actin coupled to KLH.
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Shelf Life
- 18 months from date of despatch.
- P62736 Related reagents
- Entrez Gene
- ACTA2 Related reagents
- GO Terms
- GO:0009615 response to virus
- GO:0005829 cytosol
- GO:0005524 ATP binding
- For research purposes only
Applications of Actin Alpha antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen||1/50||1/100|
|Immunohistology - Paraffin 1|
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Smooth muscle in vessels
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for Actin Alpha antibody
Skalli, O. et al. (1986) A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation.
J Cell Biol. 103 (6 Pt 2): 2787-96.
Darby, I. et al. (1990) Alpha-smooth muscle actin is transiently expressed by myofibroblasts during experimental wound healing.
Lab Invest. 63: 21-29
Desmoulière, A. et al. (1993) Transforming growth factor-beta 1 induces alpha-smooth muscle actin expression in granulation tissue myofibroblasts and in quiescent and growing cultured fibroblasts.
J Cell Biol. 122 (1): 103-11.
Hinz, B. et al. (2001) Alpha-smooth muscle actin expression upregulates fibroblast contractile activity.
Mol Biol Cell. 12 (9): 2730-41.
Hinz, B. et al. (2002) The NH2-terminal peptide of alpha-smooth muscle actin inhibits force generation by the myofibroblast in vitro and in vivo.
J Cell Biol. 157 (4): 657-63.
Shin, M.O. et al. (2010) Effect of dietary supplementation of grape skin and seeds on liver fibrosis induced by dimethylnitrosamine in rats.
Nutr Res Pract. 4: 369-74.
Brisset, A.C. et al. (2007) Intimal smooth muscle cells of porcine and human coronary artery express S100A4, a marker of the rhomboid phenotype in vitro.
Circ Res. 100: 1055-62.
Chaponnier, C. & Gabbiani, G. (2016) Monoclonal antibodies against muscle actin isoforms: epitope identification and analysis of isoform expression by immunoblot and immunostaining in normal and regenerating skeletal muscle.
F1000Res. 5: 416.
Stammler, A. et al. (2015) Epididymitis: ascending infection restricted by segmental boundaries.
Hum Reprod. 30 (7): 1557-65.