Actin Alpha antibody | 1A4
Mouse anti Human alpha actin antibody, clone 1A4 (MCA5781GA) used to demonstrate actin isoform expression in adult rat skeletal muscle by immunofluorescence.
Image caption:
Regenerating rat adult skeletal muscle co-stained with isoform specific antibodies.
C) At 4d–5d post-injury, co-staining with anti-α-SMA (red) and anti-α-CAA (green) shows that during the healing process, only a few fibers co-express both isoforms after 4d (a–c, arrowhead), whereas after 5d, only α-CAA positive fibers are detected (d–f). After 4d, myofibroblasts might participate to the repair process and are detected by using the anti-α- SMA mAb (a–c, arrow). Merged images are shown on right column. Bars = 50μm.
From: Chaponnier C, Gabbiani G.
Monoclonal antibodies against muscle actin isoforms: epitope identification and analysis of isoform expression by immunoblot and immunostaining in normal and regenerating skeletal muscle.
F1000Res. 2016, 5: 416.
Mouse anti Human actin alpha (smooth muscle) antibody, clone 1A4 (MCA5781GA) used to evaluate actin exptession in murine renal tissue by immunohistochemistry on cryosections.
Image caption:
SH045 impact on renal fibrogenesis after UUO. (E) Representative images of control and UUO-injured kidneys stained with α-SMA (magnification: 400×). Scale bars are 50 μm. (F) Quantification of α-SMA+ staining (control n = 6, UUO n = 8). Data expressed as means ± SD. Two-way ANOVA followed by Sidak’s multiple comparisons post hoc test. * p <0.05, ** p <0.01, and **** p <0.0001 defined as significant. ns, not statistically significant.
From: Zheng Z, Xu Y, Krügel U, Schaefer M, Grune T, Nürnberg B, Köhler MB, Gollasch M, Tsvetkov D, Markó L.
In Vivo Inhibition of TRPC6 by SH045 Attenuates Renal Fibrosis in a New Zealand Obese (NZO) Mouse Model of Metabolic Syndrome.
Int J Mol Sci. 2022 Jun 20;23(12):6870.
doi: 10.3390/ijms23126870.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human Actin Alpha (Smooth Muscle)
- Product Type
- Monoclonal Antibody
- Clone
- 1A4
- Isotype
- IgG2a
- Specificity
- Actin Alpha
- Region
- (SMOOTH MUSCLE)
Antibody Quality and Performance Guarantee
| Mouse anti human actin alpha (smooth muscle) antibody, clone 1A4 recognises alpha smooth muscle actin. Actins are highly conserved proteins that play a role in cell contractile activity, motility, cytokinesis, and in maintaining cell structure. Alpha smooth muscle actin is expressed in vascular smooth muscle, myofibroblasts and myoepithelial cells and also in tumours derived from these cells. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Pig Rabbit Rat Mouse Sheep - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Antibody purified from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Acetylated N-terminal synthetic decapeptide of alpha smooth muscle actin coupled to KLH.
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Immunofluorescence | |
||
| Immunohistology - Frozen | |
1/50 | 1/100 |
| Immunohistology - Paraffin 1 | |
||
| Western Blotting | |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Smooth muscle in vessels
References for Actin Alpha antibody
-
Rønnov-jessen, L. & Petersen, O.W. (1996) A function for filamentous alpha-smooth muscle actin: retardation of motility in fibroblasts.
J Cell Biol. 134 (1): 67-80. -
Skalli, O. et al. (1986) A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation.
J Cell Biol. 103 (6 Pt 2): 2787-96. -
Darby, I. et al. (1990) Alpha-smooth muscle actin is transiently expressed by myofibroblasts during experimental wound healing.
Lab Invest. 63: 21-29 -
Desmoulière, A. et al. (1993) Transforming growth factor-beta 1 induces alpha-smooth muscle actin expression in granulation tissue myofibroblasts and in quiescent and growing cultured fibroblasts.
J Cell Biol. 122 (1): 103-11. -
Hinz, B. et al. (2001) Alpha-smooth muscle actin expression upregulates fibroblast contractile activity.
Mol Biol Cell. 12 (9): 2730-41. -
Hinz, B. et al. (2002) The NH2-terminal peptide of alpha-smooth muscle actin inhibits force generation by the myofibroblast in vitro and in vivo.
J Cell Biol. 157 (4): 657-63. -
Shin, M.O. et al. (2010) Effect of dietary supplementation of grape skin and seeds on liver fibrosis induced by dimethylnitrosamine in rats.
Nutr Res Pract. 4: 369-74. -
Brisset, A.C. et al. (2007) Intimal smooth muscle cells of porcine and human coronary artery express S100A4, a marker of the rhomboid phenotype in vitro.
Circ Res. 100: 1055-62.
View The Latest Product References
-
Chaponnier, C. & Gabbiani, G. (2016) Monoclonal antibodies against muscle actin isoforms: epitope identification and analysis of isoform expression by immunoblot and immunostaining in normal and regenerating skeletal muscle.
F1000Res. 5: 416. -
Stammler, A. et al. (2015) Epididymitis: ascending infection restricted by segmental boundaries.
Hum Reprod. 30 (7): 1557-65. -
Sievers, H. et al. (2020) Pododermal angioarchitecture in the equine hoof wall: A light and scanning electron microscopic study of the wall proper.
Clin Hemorheol Microcirc. 74 (1): 21-44. -
Zheng, Z. et al. (2022) In Vivo. Inhibition of TRPC6 by SH045 Attenuates Renal Fibrosis in a New Zealand Obese (NZO) Mouse Model of Metabolic Syndrome.
Int J Mol Sci. 23 (12): 6870.
- UniProt
- P62736
- Entrez Gene
- ACTA2
- GO Terms
- GO:0009615 response to virus
- GO:0005829 cytosol
- GO:0005524 ATP binding
MCA5781GA
150459
162016
165935
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