Actin Alpha antibody | 1A4

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Mouse anti Human Actin Alpha (Smooth Muscle)

Product Type
Monoclonal Antibody
Clone
1A4
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA5781GA C E IF P* WB 0.1 mg
Mouse anti human actin alpha (smooth muscle) antibody, clone 1A4 recognises alpha smooth muscle actin. Actins are highly conserved proteins that play a role in cell contractile activity, motility, cytokinesis, and in maintaining cell structure. Alpha smooth muscle actin is expressed in vascular smooth muscle, myofibroblasts and myoepithelial cells and also in tumours derived from these cells.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Pig
Rabbit
Rat
Mouse
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Acetylated N-terminal synthetic decapeptide of alpha smooth muscle actin coupled to KLH.
Approx. Protein Concentrations
IgG concentration 1 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P62736 Related reagents
Entrez Gene
ACTA2 Related reagents
GO Terms
GO:0009615 response to virus
GO:0005829 cytosol
GO:0005524 ATP binding
Regulatory
For research purposes only

Applications of Actin Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunofluorescence
Immunohistology - Frozen 1/50 1/100
Immunohistology - Paraffin 1
Western Blotting
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Smooth muscle in vessels

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Application Based External Images

Immunofluorescence

Immunohistology - Paraffin

Western Blotting

Product Specific References

References for Actin Alpha antibody

  1. Skalli, O. et al. (1986) A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation.
    J. Cell Biol. 103: 2787-96
  2. Darby, I. et al. (1990) Alpha-smooth muscle actin is transiently expressed by myofibroblasts during experimental wound healing.
    Lab Invest. 63: 21-29
  3. Desmoulière, A. et al. (1993) Transforming growth factor-beta 1 induces alpha-smooth muscle actin expression in granulation tissue myofibroblasts and in quiescent and growing cultured fibroblasts.
    J Cell Biol. 122 (1): 103-11.
  4. Hinz, B. et al. (2001) Alpha-smooth muscle actin expression upregulates fibroblast contractile activity.
    Mol Biol Cell. 12 (9): 2730-41.
  5. Hinz, B. et al. (2002) The NH2-terminal peptide of alpha-smooth muscle actin inhibits force generation by the myofibroblast in vitro and in vivo.
    J Cell Biol. 157 (4): 657-63.
  6. Shin, M.O. et al. (2010) Effect of dietary supplementation of grape skin and seeds on liver fibrosis induced by dimethylnitrosamine in rats.
    Nutr Res Pract. 4: 369-74.
  7. Brisset, A.C. et al. (2007) Intimal smooth muscle cells of porcine and human coronary artery express S100A4, a marker of the rhomboid phenotype in vitro.
    Circ Res. 100: 1055-62.
  8. Chaponnier, C. & Gabbiani, G. (2016) Monoclonal antibodies against muscle actin isoforms: epitope identification and analysis of isoform expression by immunoblot and immunostaining in normal and regenerating skeletal muscle.
    F1000Res. 5: 416.
  9. Stammler, A. et al. (2015) Epididymitis: ascending infection restricted by segmental boundaries.
    Hum Reprod. 30 (7): 1557-65.