ACTH antibody | 57 (BGN/1388/66)

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Mouse anti Human ACTH

Product Type
Monoclonal Antibody
Clone
57 (BGN/1388/66)
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
0178-0149 E P* 0.2 mg
0178-0149G E IF P* 1 mg
Mouse anti Human ACTH antibody (Clone 57, BGN/1388/66) recognizes 1-24 ACTH (Synacthen) which, as a synthetic analogue of naturally-occurring Adrenocorticotropic Hormone, can be used to measure adrenal reserve and for the diagnosis of adrenal insufficiency by acute adrenocortical stimulation.

ACTH, released from the anterior pituitary gland in response to corticotropin-releasing hormone from the hypothalamus, acts on the adrenal cortex to stimulate the production of corticosteroids such as cortisol, involved in the response to stress. Studies have shown that the administration of 1-24 ACTH increases blood pressure, believed to be attributed to an increase in ACTH-stimulated cortisol secretion, in association with increased cardiac output.

Mouse anti Human ACTH antibody does not recognize 17-39 ACTH (CLIP). It does cross-reacts with rat N-terminal ACTH.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Synthetic peptide corresponding to the amino acids 1 - 24 (SYSMEHFRWGKPVGKKRRPVKVYP) of the human ACTH conjugated to Keyhole Limpet Haemocyanin.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P01189 Related reagents
Entrez Gene
POMC Related reagents
GO Terms
GO:0005179 hormone activity
GO:0005615 extracellular space
GO:0030141 stored secretory granule
GO:0006091 generation of precursor metabolites and energy
GO:0007218 neuropeptide signaling pathway
GO:0007267 cell-cell signaling
GO:0008217 regulation of blood pressure
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0016486 peptide hormone processing
GO:0031781 type 3 melanocortin receptor binding
GO:0031782 type 4 melanocortin receptor binding
GO:0032098 regulation of appetite
GO:0032720 negative regulation of tumor necrosis factor production
GO:0033059 cellular pigmentation
GO:0034641 cellular nitrogen compound metabolic process
GO:0042446 hormone biosynthetic process
GO:0070996 type 1 melanocortin receptor binding
Regulatory
For research purposes only

Applications of ACTH antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunofluorescence
Immunohistology - Paraffin 1 1/100 1/200
  1. 1 This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Histar Detection System STAR3000A 50 Tests C P
Histar Detection System STAR3000B 150 Tests C P
Histar Detection System STAR3000C 500 Tests C P

Product Specific References

References for ACTH antibody

  1. Zhou, M et al. (2016) Localization of ATP-sensitive K+ channel subunits in rat pituitary gland.
    Arch Histol Cytol. 76 (1): 53-66.

Further Reading

  1. Hatakeyama, H. et al. (2000) Functional adrenocorticotropic hormone receptor in cultured human vascular endothelial cells : possible role in control of blood pressure.
    Hypertension. 36 (5): 862-5.