IgE antibody | 3H10
Mouse anti Horse IgE, clone 3H10, recognizes native equine IgE and does not cross react with equine IgM, IgA or IgG.
IgE is an immunoglobulin primarily produced from plasma cells and, in normal serum, present at very low concentrations.
IgE is important in both type 1 hypersensitivity and immunity to parasite infections, in particular parasitic worms where equine IgE levels are significantly elevated following infection.
Monoclonal antibodies to equine IgE are of particular relevance to research into insect bite sensitivity, one of the most widely studied allergic diseases in equid species (Schaffartzik, A. et al. 2012).
IgE is an immunoglobulin primarily produced from plasma cells and, in normal serum, present at very low concentrations.
IgE is important in both type 1 hypersensitivity and immunity to parasite infections, in particular parasitic worms where equine IgE levels are significantly elevated following infection.
Monoclonal antibodies to equine IgE are of particular relevance to research into insect bite sensitivity, one of the most widely studied allergic diseases in equid species (Schaffartzik, A. et al. 2012).
Product Details
- Target Species
- Horse
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
HRP Stabiliser (BUF052A) - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Equine IgE
- Approx. Protein Concentrations
- IgG concentration1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the P3X myeloma cell line
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of IgE antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/500 | 1/5,000 | |
| ELISA | 1/1000 | 1/10,000 | |
| Immunohistology - Frozen | |||
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- This product may be used as a detection reagent in a sandwich ELISA together with MCA5983GA as the capture reagent.
- ELISA
- This product is suitable for use in indirect ELISA applications.
- Western Blotting
- Western blot analysis against affinity purified equine IgE using Mouse anti Horse IgE clone 3H10 demonstrates a band of approximately 80 kDa under reducing conditions and a band of approximately 200 kDa under non-reducing conditions which correspond with the expected molecular weight of equine Ig epsilon chain and the complete equine IgE molecule, respectively (Wilson, D.A. et al. 2006).
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Secondary Antibodies Available
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti Horse IgE | MCA5983GA | C E P* WB* | 0.1 mg |
|
|
| Mouse anti Horse IgE | MCA5983GA | C E P* WB* | 0.1 mg |
|
Product Specific References
Source Reference
-
Wilson, D.A. et al. (2006) Production of monoclonal antibodies specific for native equine IgE and their application to monitor total serum IgE responses in Icelandic and non-Icelandic horses with insect bite dermal hypersensitivity.
Vet Immunol Immunopathol.112: 56-70.
Further Reading
-
Schaffartzik, A.et al. (2012) Equine insect bite hypersensitivity: what do we know?
Vet Immunol Immunopathol. 147: 113-26.
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