CD14 antibody | 105



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F ResetMouse anti Horse CD14
- Product Type
- Monoclonal Antibody
- Clone
- 105
- Isotype
- IgG1
- Specificity
- CD14
Mouse anti Horse CD14 antibody, clone 105 recognizes horse CD14. CD14 is a GPI-anchored membrane glycoprotein and monocyte/macrophage differentiation antigen. CD14 belongs to the lipopolysaccharide receptor family and is weakly expressed on microglia and Langerhans cells. CD14 acts as a receptor for the potent bacterial endotoxin, lipopolysaccharide (LPS), facilitated by LPS-binding protein (LBP). The binding of LPS to CD14 results in cell activation and the release of cytokines and the inflammatory response, and has been shown to upregulate the cell surface expression of adhesion molecules. Mouse anti Horse CD14 antibody, clone 105, will detect approx. 7.7% of horse PBMC (Kabithe et al. 2010). |
- Target Species
- Horse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Recombinant equine CD14.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the X3.Ag8-653 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | ![]() |
1/50 | 1/200 |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
References for CD14 antibody
-
Perkins, G.A. et al. (2014) Maternal T-lymphocytes in equine colostrum express a primarily inflammatory phenotype.
Vet Immunol Immunopathol. 161 (3-4): 141-50. -
Ziegler, A. et al. (2016) Equine dendritic cells generated with horse serum have enhanced functionality in comparison to dendritic cells generated with fetal bovine serum.
BMC Vet Res. 12 (1): 254. -
Larson, E.M. et al. (2020) Phenotype and function of IgE-binding monocytes in equine Culicoides hypersensitivity.
PLoS One. 15 (5): e0233537. -
Raza, F. et al. (2021) Peripheral blood basophils are the main source for early interleukin-4 secretion upon in vitro stimulation with Culicoides allergen in allergic horses.
PLoS One. 16 (5): e0252243. -
Tukia, E. et al. (2021) The Effect of Uterine Lavage on Soluble CD14, Chemokine Ligand 2, and Interleukin 10 Levels in Mares With Postpartum Metritis.
J Equine Vet Sci. 98: 103365. -
Gressler, A.E. et al. (2022) Comprehensive Flow Cytometric Characterization of Bronchoalveolar Lavage Cells Indicates Comparable Phenotypes Between Asthmatic and Healthy Horses But Functional Lymphocyte Differences.
Front Immunol. 13: 896255.
Further Reading
-
Kabithe, E. et al. (2010) Monoclonal antibodies to equine CD14.
Vet Immunol Immunopathol. 138 (1-2): 149-53.
- UniProt
- Q9TTT3
MCA6393GA



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