CD14 antibody | 105

Mouse anti Horse CD14

Product Type
Monoclonal Antibody
Clone
105
Isotype
IgG1
Specificity
CD14

Product Code Applications Pack Size List Price Your Price Qty
New MCA6393GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Horse CD14 antibody, clone 105 recognizes horse CD14.

CD14 is a GPI-anchored membrane glycoprotein and monocyte/macrophage differentiation antigen. CD14 belongs to the lipopolysaccharide receptor family and is weakly expressed on microglia and Langerhans cells. CD14 acts as a receptor for the potent bacterial endotoxin, lipopolysaccharide (LPS), facilitated by LPS-binding protein (LBP). The binding of LPS to CD14 results in cell activation and the release of cytokines and the inflammatory response, and has been shown to upregulate the cell surface expression of adhesion molecules.

Mouse anti Horse CD14 antibody, clone 105, will detect approx. 7.7% of horse PBMC (Kabithe et al. 2010).

Target Species
Horse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Recombinant equine CD14
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the X3.Ag8-653 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/200
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

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References for CD14 antibody

  1. Wagner, B. & Freer, H. (2009) Development of a bead-based multiplex assay for simultaneous quantification of cytokines in horses.
    Vet Immunol Immunopathol. 127 (3-4): 242-8.
  2. Perkins, G.A. et al. (2014) Maternal T-lymphocytes in equine colostrum express a primarily inflammatory phenotype.
    Vet Immunol Immunopathol. 161 (3-4): 141-50.

Further Reading

  1. Kabithe, E. et al. (2010) Monoclonal antibodies to equine CD14.
    Vet Immunol Immunopathol. 138 (1-2): 149-53.

UniProt
Q9TTT3
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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