CD14 antibody | 105
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Mouse anti Horse CD14
- Product Type
- Monoclonal Antibody
- Clone
- 105
- Isotype
- IgG1
- Specificity
- CD14
Antibody Quality and 
Unrivaled 
MCA6393GA
| Mouse anti Horse CD14 antibody, clone 105 recognizes horse CD14. CD14 is a GPI-anchored membrane glycoprotein and monocyte/macrophage differentiation antigen. CD14 belongs to the lipopolysaccharide receptor family and is weakly expressed on microglia and Langerhans cells. CD14 acts as a receptor for the potent bacterial endotoxin, lipopolysaccharide (LPS), facilitated by LPS-binding protein (LBP). The binding of LPS to CD14 results in cell activation and the release of cytokines and the inflammatory response, and has been shown to upregulate the cell surface expression of adhesion molecules. Mouse anti Horse CD14 antibody, clone 105, will detect approx. 7.7% of horse PBMC (Kabithe et al. 2010). |
- Target Species
- Horse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Recombinant equine CD14
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the X3.Ag8-653 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/200 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
References for CD14 antibody
-
Wagner, B. & Freer, H. (2009) Development of a bead-based multiplex assay for simultaneous quantification of cytokines in horses.
Vet Immunol Immunopathol. 127 (3-4): 242-8. -
Perkins, G.A. et al. (2014) Maternal T-lymphocytes in equine colostrum express a primarily inflammatory phenotype.
Vet Immunol Immunopathol. 161 (3-4): 141-50.
Further Reading
-
Kabithe, E. et al. (2010) Monoclonal antibodies to equine CD14.
Vet Immunol Immunopathol. 138 (1-2): 149-53.
- UniProt
- Q9TTT3
View more products with CD14 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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