Heparin/Heparan sulfate antibody | T320.11

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Mouse anti Heparin/Heparan sulfate

Product Type
Monoclonal Antibody
Clone
T320.11
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
OBT1698
Datasheet
E R WB
MSDS
0.1 mg loader

Mouse anti Heparin/Heparan sulfate, clone T320.11, recognizes the chemically related αβ-linked glycosaminoglycans heparin and heparan sulfate.

Both heparin and heparan sulfate are composed of alternating sequences of glucosamine and uronic acid with heparin being the more heavily sulfated polymer (Gallagher & Walker, 1985).

Product Details

Target Species
Broad
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate Buffered Saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of Heparin/Heparan sulfate antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1 10ug/ml
Radioimmunoassays 1 10ug/ml
Western Blotting 1/500 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Product Specific References

References for Heparin/Heparan sulfate antibody

  1. Shibata, S. et al. (1993) Monoclonal antibodies to heparan sulfate inhibit the formation of thrombin-antithrombin III complexes.
    Clin Immunol Immunopathol. 67 (3 Pt 1): 264-72.
  2. Coles, C.H. et al. (2011) Proteoglycan-specific molecular switch for RPTPσ clustering and neuronal extension.
    Science. 332 (6028): 484-8.
  3. Kato, R. et al. (2017) Heparan sulfate storage in the cardiac conduction system triggers atrioventricular block.
    Brain Dev. 39 (5): 418-421.

Further Reading

  1. Gallagher, J.T. & Walker, A. (1985) Molecular distinctions between heparan sulphate and heparin. Analysis of sulphation patterns indicates that heparan sulphate and heparin are separate families of N-sulphated polysaccharides.
    Biochem J. 230 (3): 665-74.