Heparin/Heparan sulfate antibody | T320.11
Mouse anti heparin/heparan sulfate antibody, clone T320.11 (OBT1698) used to functionalize cantilever tips for the analysis of skin samples by atomic force microscopy.
Image caption:
Heparan sulfate mapping of the stripped abdominal skin sample using a cantilever tip functionalized with the anti‐heparan sulfate antibody. The scan size is 1 μm × 1 μm. (A) Topography image. The height scale is 12 nm for the full color range. (B) Simultaneously acquired recognition map. Blue to orange reflects no recognition and dark red corresponds to a recognition event. (C) An overlay of the topography and the recognition map showed that heparan sulfate is located on surface protrusions (bead‐like structures, indicated with arrows).
From: Danzberger J, Donovan M, Rankl C, Zhu R, Vicic S, Baltenneck C, Enea R, Hinterdorfer P, Luengo GS.
Glycan distribution and density in native skin's stratum corneum.
Skin Res Technol. 2018 Aug;24(3):450-58.
doi: 10.1111/srt.12453.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti heparin/heparan sulfate antibody, clone T320.11 (OBT1698) used to functionalize cantilever tips for the analysis of skin samples by atomic force microscopy.
Image caption:
Dynamic force spectroscopy of the anti‐heparan sulfate antibody and its cognate cell receptor. The data for the single arm rupture (cyan squares) were fitted with the single energy barrier model (cyan line). Data of double‐arm ruptures (magenta squares) were fitted using a 2‐bond Markov model (magenta line) with the acquired xB and koff parameters from the single arm rupture fit
From: Danzberger J, Donovan M, Rankl C, Zhu R, Vicic S, Baltenneck C, Enea R, Hinterdorfer P, Luengo GS.
Glycan distribution and density in native skin's stratum corneum.
Skin Res Technol. 2018 Aug;24(3):450-58.
doi: 10.1111/srt.12453.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Heparin/Heparan sulfate
- Product Type
- Monoclonal Antibody
- Clone
- T320.11
- Isotype
- IgG1
- Specificity
- Heparin/Heparan sulfate
Antibody Quality and Performance Guarantee
| Mouse anti Heparin/Heparan sulfate, clone T320.11, recognizes the chemically related αβ-linked glycosaminoglycans heparin and heparan sulfate. Both heparin and heparan sulfate are composed of alternating sequences of glucosamine and uronic acid with heparin being the more heavily sulfated polymer (Gallagher & Walker, 1985). |
- Target Species
- Broad
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- Phosphate Buffered Saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1 | 10ug/ml |
| Radioimmunoassays | |
1 | 10ug/ml |
| Western Blotting | |
1/500 | 1/1000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for Heparin/Heparan sulfate antibody
-
Coles, C.H. et al. (2011) Proteoglycan-specific molecular switch for RPTPσ clustering and neuronal extension.
Science. 332 (6028): 484-8. -
Kato, R. et al. (2017) Heparan sulfate storage in the cardiac conduction system triggers atrioventricular block.
Brain Dev. 39 (5): 418-421. -
Danzberger, J. et al. (2018) Glycan distribution and density in native skin's stratum corneum.
Skin Res Technol. 24 (3): 450-8.
Further Reading
-
Gallagher, J.T. & Walker, A. (1985) Molecular distinctions between heparan sulphate and heparin. Analysis of sulphation patterns indicates that heparan sulphate and heparin are separate families of N-sulphated polysaccharides.
Biochem J. 230 (3): 665-74. -
Shibata, S. et al. (1993) Monoclonal antibodies to heparan sulfate inhibit the formation of thrombin-antithrombin III complexes.
Clin Immunol Immunopathol. 67 (3 Pt 1): 264-72.
- RRID
- AB_617290
OBT1698
153537
159891
165353
166447
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