T Lymphocytes antibody | MsGp7
FITC conjugated Mouse anti Guinea Pig T lymphocyte antibody, clone MsGp7 (MCA564F) used to identify guinea pig lymphocytes by flow cytometry.
Image caption:
BCG-vaccination elicits CD1b-restricted, PIM6-specific T cell-responses. Four weeks after BCG-vaccination PBMCs were isolated. Autologous, CD1b-expressing APCs and non-adherent lymphocytes were stained with CFSE and stimulated with mycobacterial antigens. CFSE dilution was analyzed by flow-cytometry after five days. (B) CFSE-low, PIM6-reactive lymphocytes were stained for a general T cell-marker, CD4 and CD8. The small panel at the left shows the gating strategy. The larger pseudo-color graph representatively shows the CD4- and CD8-distribution. The dot blot on the right shows the relative distribution for T cell-markers in PIM6-reactive cells four weeks after BCG-vaccination. Red symbols represent the normal T cell- and CD4-CD8-distribution in PBMCs from naïve control guinea pigs. Asterisks indicate the level of significance as determined by unpaired t-test.
From: Eckhardt E, Schinköthe J, Gischke M, Sehl-Ewert J, Corleis B, Dorhoi A, Teifke J, Albrecht D, Geluk A, Gilleron M, Bastian M.
Phosphatidylinositolmannoside vaccination induces lipid-specific Th1-responses and partially protects guinea pigs from Mycobacterium tuberculosis challenge.
Sci Rep. 2023 Oct 30;13(1):18613.
doi: 10.1038/s41598-023-45898-3.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
F ResetMouse anti Guinea Pig T Lymphocytes
- Product Type
- Monoclonal Antibody
- Clone
- MsGp7
- Isotype
- IgG2a
- Specificity
- T Lymphocytes
Antibody Quality and Performance Guarantee
| Mouse anti Guinea Pig T Lymphocytes, clone MsGp7, is pan reactive with guinea pig T lymphocytes (>95%) in lymph nodes, and does not react with B lymphocytes. It reacts with approximately 70% of thymocytes by FACS analysis and immunohistochemically the medullary thymocytes strongly express this antigen (Healey et al. 1988). |
- Target Species
- Guinea Pig
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Guinea Pig T Lymphocytes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/200 |
| Immunohistology - Frozen | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse anti Guinea Pig Macrophages | MCA518S | C F * | 2 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Mouse anti Guinea Pig Macrophages | ||||||
| Mouse anti Guinea Pig B Cell Subset | MCA567 | C F | 2 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Mouse anti Guinea Pig B Cell Subset | ||||||
| Mouse anti Guinea Pig CD8:FITC | MCA752F | F | 100 Tests |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Mouse anti Guinea Pig CD8:FITC | ||||||
References for T Lymphocytes antibody
-
Healey, D.G. et al. (1988) Behaviour of guinea pig T cells stimulated by antigen, allo-antigen and mitogen.
Int Arch Allergy Appl Immunol. 87 (2): 134-42. -
Butter, C. et al. (1988) An immunoelectron microscopical study of the expression of class II MHC and a T lymphocyte surface marker during chronic relapsing experimental allergic encephalomyelitis.
J Neuroimmunol. 20 (1): 45-51. -
Cowley, S.A. et al. (1989) An immunoelectronmicroscopical study of the expression of major histocompatibility complex (MHC) class II antigens in guinea pig sciatic nerves following induction of intraneural mycobacterial granulomas.
J Neuroimmunol. 23 (3): 223-31. -
Sato H et al. (1997) Production of murine monoclonal antibodies to guinea pig leukocytes and immunohistochemistry of guinea pig skin exposed to Schistosoma mansoni.
Hybridoma. 16 (6): 529-36. -
Kaufmann, E. et al. (2016) BCG Vaccination Induces Robust CD4+ T Cell Responses to Mycobacterium tuberculosis Complex-Specific Lipopeptides in Guinea Pigs.
J Immunol. 196 (6): 2723-32. -
Eckhardt, E. et al. (2023) Phosphatidylinositolmannoside vaccination induces lipid-specific Th1-responses and partially protects guinea pigs from Mycobacterium tuberculosis challenge.
Sci Rep. 13 (1): 18613.
Further Reading
-
Schäfer H & Burger R (2012) Tools for cellular immunology and vaccine research the in the guinea pig: monoclonal antibodies to cell surface antigens and cell lines.
Vaccine. 30 (40): 5804-11.
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