T Lymphocytes antibody | MsGp7
Mouse anti Guinea Pig T Lymphocytes, clone MsGp7, is pan reactive with guinea pig T lymphocytes (>95%) in lymph nodes, and does not react with B lymphocytes. It reacts with approximately 70% of thymocytes by FACS analysis and immunohistochemically the medullary thymocytes strongly express this antigen (Healey et al. 1988).
Product Details
- Target Species
- Guinea Pig
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Guinea Pig T Lymphocytes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. - Shelf Life
- 18 months from date of despatch.
More Information
- Regulatory
- For research purposes only
Applications of T Lymphocytes antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/50 | 1/200 | |
| Immunohistology - Frozen |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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Secondary Antibodies Available
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti Guinea Pig Macrophages | MCA518S | 2 ml | C F* | ||
| Mouse anti Guinea Pig B Cell Subset | MCA567 | 2 ml | C F | ||
| Mouse anti Guinea Pig CD8:FITC | MCA752F | 100 Tests | F |
Product Specific References
References for T Lymphocytes antibody
-
Healey, D.G. et al. (1988) Behaviour of guinea pig T cells stimulated by antigen, allo-antigen and mitogen.
Int Arch Allergy Appl Immunol. 87 (2): 134-42. -
Butter, C. et al. (1988) An immunoelectron microscopical study of the expression of class II MHC and a T lymphocyte surface marker during chronic relapsing experimental allergic encephalomyelitis.
J Neuroimmunol. 20 (1): 45-51. -
Kaufmann, E. et al. (2016) BCG Vaccination Induces Robust CD4+ T Cell Responses to Mycobacterium tuberculosis Complex-Specific Lipopeptides in Guinea Pigs.
J Immunol. 196 (6): 2723-32. -
Sato H et al. (1997) Production of murine monoclonal antibodies to guinea pig leukocytes and immunohistochemistry of guinea pig skin exposed to Schistosoma mansoni.
Hybridoma. 16 (6): 529-36. -
Cowley, S.A. et al. (1989) An immunoelectronmicroscopical study of the expression of major histocompatibility complex (MHC) class II antigens in guinea pig sciatic nerves following induction of intraneural mycobacterial granulomas.
J Neuroimmunol. 23 (3): 223-31. -
Schäfer H & Burger R (2012) Tools for cellular immunology and vaccine research the in the guinea pig: monoclonal antibodies to cell surface antigens and cell lines.
Vaccine. 30 (40): 5804-11.
