T Lymphocytes antibody | MsGp7
Mouse anti Guinea Pig T Lymphocytes, clone MsGp7, is pan reactive with guinea pig T lymphocytes (>95%) in lymph nodes, and does not react with B lymphocytes. It reacts with approximately 70% of thymocytes by FACS analysis and immunohistochemically the medullary thymocytes strongly express this antigen, see Healey D.G. et al. for details. The functional significance of this antigen is currently unknown.
Product Details
- Target Species
- Guinea Pig
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Guinea Pig T Lymphocytes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. - Shelf Life
- 18 months from date of despatch.
More Information
- Regulatory
- For research purposes only
Applications of T Lymphocytes antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/50 | 1/200 | |
| Immunohistology - Frozen |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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Secondary Antibodies Available
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti Guinea Pig Macrophages | MCA518S | 2 ml | C F* | ||
| Mouse anti Guinea Pig B Cell Subset | MCA567 | 2 ml | C F | ||
| Mouse anti Guinea Pig CD8:FITC | MCA752F | 100 Tests | F |
Product Specific References
References for T Lymphocytes antibody
-
Healey, D.G. et al. (1988) Behaviour of guinea pig T cells stimulated by antigen, allo-antigen and mitogen.
Int Arch Allergy Appl Immunol. 87 (2): 134-42. -
Butter, C. et al. (1988) An immunoelectron microscopical study of the expression of class II MHC and a T lymphocyte surface marker during chronic relapsing experimental allergic encephalomyelitis.
J Neuroimmunol. 20 (1): 45-51. -
Kaufmann, E. et al. (2016) BCG Vaccination Induces Robust CD4+ T Cell Responses to Mycobacterium tuberculosis Complex-Specific Lipopeptides in Guinea Pigs.
J Immunol. 196 (6): 2723-32. -
Sato H et al. (1997) Production of murine monoclonal antibodies to guinea pig leukocytes and immunohistochemistry of guinea pig skin exposed to Schistosoma mansoni.
Hybridoma. 16 (6): 529-36. -
Cowley, S.A. et al. (1989) An immunoelectronmicroscopical study of the expression of major histocompatibility complex (MHC) class II antigens in guinea pig sciatic nerves following induction of intraneural mycobacterial granulomas.
J Neuroimmunol. 23 (3): 223-31. -
Schäfer H & Burger R (2012) Tools for cellular immunology and vaccine research the in the guinea pig: monoclonal antibodies to cell surface antigens and cell lines.
Vaccine. 30 (40): 5804-11.
