T Lymphocytes antibody | MsGp7

Mouse anti Guinea Pig T Lymphocytes

Product Type
Monoclonal Antibody
Clone
MsGp7
Isotype
IgG2a
Specificity
T Lymphocytes

Product Code Applications Pack Size List Price Your Price Qty
MCA564GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F 0.1 mg loader
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loader
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Mouse anti Guinea Pig T Lymphocytes, clone MsGp7, is pan reactive with guinea pig T lymphocytes (>95%) in lymph nodes, and does not react with B lymphocytes. It reacts with approximately 70% of thymocytes by FACS analysis and immunohistochemically the medullary thymocytes strongly express this antigen (Healey et al. 1988).

Target Species
Guinea Pig
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Guinea Pig T Lymphocytes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/200
Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

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References for T Lymphocytes antibody

  1. Healey, D.G. et al. (1988) Behaviour of guinea pig T cells stimulated by antigen, allo-antigen and mitogen.
    Int Arch Allergy Appl Immunol. 87 (2): 134-42.
  2. Butter, C. et al. (1988) An immunoelectron microscopical study of the expression of class II MHC and a T lymphocyte surface marker during chronic relapsing experimental allergic encephalomyelitis.
    J Neuroimmunol. 20 (1): 45-51.
  3. Kaufmann, E. et al. (2016) BCG Vaccination Induces Robust CD4+ T Cell Responses to Mycobacterium tuberculosis Complex-Specific Lipopeptides in Guinea Pigs.
    J Immunol. 196 (6): 2723-32.
  4. Sato H et al. (1997) Production of murine monoclonal antibodies to guinea pig leukocytes and immunohistochemistry of guinea pig skin exposed to Schistosoma mansoni.
    Hybridoma. 16 (6): 529-36.
  5. Cowley, S.A. et al. (1989) An immunoelectronmicroscopical study of the expression of major histocompatibility complex (MHC) class II antigens in guinea pig sciatic nerves following induction of intraneural mycobacterial granulomas.
    J Neuroimmunol. 23 (3): 223-31.
  6. Schäfer H & Burger R (2012) Tools for cellular immunology and vaccine research the in the guinea pig: monoclonal antibodies to cell surface antigens and cell lines.
    Vaccine. 30 (40): 5804-11.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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