CD1b3 antibody | MsGP9

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Anti Guinea Pig CD1b3 Antibody, clone MsGP9 gallery image 1 — Guinea pig splenocytes stained with Mouse anti Guinea Pig CD1b3 (MCA566GA) followed by goat anti mouse IgG:FITC (STAR117F)

Mouse anti Guinea Pig CD1b3

Mouse anti Guinea Pig CD1b3:RPE

Product Type: Monoclonal Antibody
Clone: MsGP9
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA566GA	C F			0.1 mg
MCA566PE	F			100 Tests

Mouse anti Guinea Pig CD1b3 antibody, clone MsGP9 recognizes an antigen expressed by guinea pig B lymphocytes.

The antigen is found on mature B cells, weakly on germinal centre B cells, and also on a proportion of thymocytes and peritoneal macrophages.

Mouse anti Guinea Pig CD1b3 antibody, clone MsGP9 appears to recognize the guinea pig homologue of CD1b3 (Hiromatsu et al. 2001).

Product Details

Target Species

Guinea Pig

Product Form

Purified IgG - liquid

Product Form

Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized

Reconstitution

Reconstitute with 1 ml distilled water

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin
5%	Sucrose

Carrier Free

Yes

Immunogen

Guinea pig thymocytes.

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Fusion Partners

Spleen cells from an immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC.

DO NOT FREEZE

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 12 months from date of reconstitution.

More Information

UniProt: Q9QZZ0 Related reagents
Entrez Gene: Cd1b3 Related reagents
GO Terms: GO:0005886 plasma membrane; GO:0016021 integral to membrane; GO:0005765 lysosomal membrane; GO:0006955 immune response; GO:0010008 endosome membrane; GO:0019882 antigen processing and presentation
Regulatory: For research purposes only

Applications of CD1b3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry	1/50	1/200
Immunohistology - Frozen
Flow Cytometry

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse IgG1 Negative Control:RPE	MCA928PE	100 Tests	F

Application Based External Images

Flow Cytometry

Immunohistology - Frozen

Product Specific References

References for CD1b3 antibody

Butter, C. et al. (1989) A quantitative immunocytochemical study of the infiltrating lymphocytes in the spinal cord of guinea pigs with chronic relapsing experimental allergic encephalomyelitis.
J Neuroimmunol. 25 (2-3): 169-76.
Hiromatsu, K. et al. (2002) Characterization of guinea-pig group 1 CD1 proteins.
Immunology. 106 (2): 159-72.
Dascher, C.C. et al. (2002) Conservation of CD1 intracellular trafficking patterns between mammalian species.
J Immunol. 169: 6951-8.
Lanz, T.A. et al. (2010) Pharmacodynamics and pharmacokinetics of the gamma-secretase inhibitor PF-3084014.
J Pharmacol Exp Ther. 334: 269-77.
Meshi, B. et al. (2002) Emphysematous lung destruction by cigarette smoke. The effects of latent adenoviral infection on the lung inflammatory response.
Am J Respir Cell Mol Biol. 26: 52-7.
Ordway, D. et al. (2007) The cellular immune response to Mycobacterium tuberculosis infection in the guinea pig.
J Immunol. 179: 2532-41.
Ordway, D. et al. (2008) Influence of Mycobacterium bovis BCG vaccination on cellular immune response of guinea pigs challenged with Mycobacterium tuberculosis.
Clin Vaccine Immunol. 15: 1248-58.

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