CD1b3 antibody | MsGP9
Mouse anti Guinea Pig CD1b3
- Product Type
- Monoclonal Antibody
- Clone
- MsGP9
- Isotype
- IgG1
- Specificity
- CD1b3
Antibody Quality and Performance Guarantee
| Mouse anti Guinea Pig CD1b3 antibody, clone MsGP9 recognizes an antigen expressed by guinea pig B lymphocytes. The antigen is found on mature B cells, weakly on germinal centre B cells, and also on a proportion of thymocytes and peritoneal macrophages. Mouse anti Guinea Pig CD1b3 antibody, clone MsGP9 appears to recognize the guinea pig homologue of CD1b3 (Hiromatsu et al. 2001). |
- Target Species
- Guinea Pig
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Guinea pig thymocytes.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from an immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/200 |
| Immunohistology - Frozen | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Source Reference
-
Butter, C. et al. (1989) A quantitative immunocytochemical study of the infiltrating lymphocytes in the spinal cord of guinea pigs with chronic relapsing experimental allergic encephalomyelitis.
J Neuroimmunol. 25 (2-3): 169-76.
References for CD1b3 antibody
-
Meshi, B. et al. (2002) Emphysematous lung destruction by cigarette smoke. The effects of latent adenoviral infection on the lung inflammatory response.
Am J Respir Cell Mol Biol. 26: 52-7. -
Dascher, C.C. et al. (2002) Conservation of CD1 intracellular trafficking patterns between mammalian species.
J Immunol. 169: 6951-8. -
Hiromatsu, K. et al. (2002) Characterization of guinea-pig group 1 CD1 proteins.
Immunology. 106 (2): 159-72. -
Ordway, D. et al. (2007) The cellular immune response to Mycobacterium tuberculosis infection in the guinea pig.
J Immunol. 179: 2532-41. -
Ordway, D. et al. (2008) Influence of Mycobacterium bovis BCG vaccination on cellular immune response of guinea pigs challenged with Mycobacterium tuberculosis.
Clin Vaccine Immunol. 15: 1248-58. -
Lanz, T.A. et al. (2010) Pharmacodynamics and pharmacokinetics of the gamma-secretase inhibitor PF-3084014.
J Pharmacol Exp Ther. 334: 269-77.
- RRID
- AB_11152940
- UniProt
- Q9QZZ0
- Entrez Gene
- Cd1b3
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0005765 lysosomal membrane
- GO:0006955 immune response
- GO:0010008 endosome membrane
- GO:0019882 antigen processing and presentation
MCA566GA
151032
164528
166198
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