CD1b3 antibody | MsGP9
Mouse anti Guinea Pig CD1b3 antibody, clone MsGP9 recognizes an antigen expressed by guinea pig B lymphocytes.
The antigen is found on mature B cells, weakly on germinal centre B cells, and also on a proportion of thymocytes and peritoneal macrophages.
Mouse anti Guinea Pig CD1b3 antibody, clone MsGP9 appears to recognize the guinea pig homologue of CD1b3 (Hiromatsu et al. 2001).
The antigen is found on mature B cells, weakly on germinal centre B cells, and also on a proportion of thymocytes and peritoneal macrophages.
Mouse anti Guinea Pig CD1b3 antibody, clone MsGP9 appears to recognize the guinea pig homologue of CD1b3 (Hiromatsu et al. 2001).
Product Details
- Target Species
- Guinea Pig
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Immunogen
- Guinea pig thymocytes.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from an immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- Store at +4oC.
DO NOT FREEZE
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q9QZZ0
- Entrez Gene
- Cd1b3
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0005765 lysosomal membrane
- GO:0006955 immune response
- GO:0010008 endosome membrane
- GO:0019882 antigen processing and presentation
- Regulatory
- For research purposes only
Applications of CD1b3 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/50 | 1/200 | |
| Immunohistology - Frozen | |||
| Flow Cytometry |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests |
|
Product Specific References
References for CD1b3 antibody
-
Butter, C. et al. (1989) A quantitative immunocytochemical study of the infiltrating lymphocytes in the spinal cord of guinea pigs with chronic relapsing experimental allergic encephalomyelitis.
J Neuroimmunol. 25 (2-3): 169-76. -
Hiromatsu, K. et al. (2002) Characterization of guinea-pig group 1 CD1 proteins.
Immunology. 106 (2): 159-72. -
Dascher, C.C. et al. (2002) Conservation of CD1 intracellular trafficking patterns between mammalian species.
J Immunol. 169: 6951-8. -
Lanz, T.A. et al. (2010) Pharmacodynamics and pharmacokinetics of the gamma-secretase inhibitor PF-3084014.
J Pharmacol Exp Ther. 334: 269-77. -
Meshi, B. et al. (2002) Emphysematous lung destruction by cigarette smoke. The effects of latent adenoviral infection on the lung inflammatory response.
Am J Respir Cell Mol Biol. 26: 52-7. -
Ordway, D. et al. (2007) The cellular immune response to Mycobacterium tuberculosis infection in the guinea pig.
J Immunol. 179: 2532-41. -
Ordway, D. et al. (2008) Influence of Mycobacterium bovis BCG vaccination on cellular immune response of guinea pigs challenged with Mycobacterium tuberculosis.
Clin Vaccine Immunol. 15: 1248-58.
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