CD1b3 antibody | MsGP9

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Mouse anti Guinea Pig CD1b3

Mouse anti Guinea Pig CD1b3:RPE

Product Type
Monoclonal Antibody
Clone
MsGP9
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA566GA C F 0.1 mg
MCA566PE F 100 Tests
Mouse anti Guinea Pig CD1b3 antibody, clone MsGP9 recognizes an antigen expressed by guinea pig B lymphocytes.

The antigen is found on mature B cells, weakly on germinal centre B cells, and also on a proportion of thymocytes and peritoneal macrophages.

Mouse anti Guinea Pig CD1b3 antibody, clone MsGP9 appears to recognize the guinea pig homologue of CD1b3 (Hiromatsu et al. 2001).

Product Details

Target Species
Guinea Pig
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Carrier Free
Yes
Immunogen
Guinea pig thymocytes.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from an immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC.

DO NOT FREEZE

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
Q9QZZ0 Related reagents
Entrez Gene
Cd1b3 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005765 lysosomal membrane
GO:0006955 immune response
GO:0010008 endosome membrane
GO:0019882 antigen processing and presentation
Regulatory
For research purposes only

Applications of CD1b3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/200
Immunohistology - Frozen
Flow Cytometry
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control:RPE MCA928PE 100 Tests F

Application Based External Images

Flow Cytometry

Immunohistology - Frozen

Product Specific References

References for CD1b3 antibody

  1. Butter, C. et al. (1989) A quantitative immunocytochemical study of the infiltrating lymphocytes in the spinal cord of guinea pigs with chronic relapsing experimental allergic encephalomyelitis.
    J Neuroimmunol. 25 (2-3): 169-76.
  2. Hiromatsu, K. et al. (2002) Characterization of guinea-pig group 1 CD1 proteins.
    Immunology. 106 (2): 159-72.
  3. Dascher, C.C. et al. (2002) Conservation of CD1 intracellular trafficking patterns between mammalian species.
    J Immunol. 169: 6951-8.
  4. Lanz, T.A. et al. (2010) Pharmacodynamics and pharmacokinetics of the gamma-secretase inhibitor PF-3084014.
    J Pharmacol Exp Ther. 334: 269-77.
  5. Meshi, B. et al. (2002) Emphysematous lung destruction by cigarette smoke. The effects of latent adenoviral infection on the lung inflammatory response.
    Am J Respir Cell Mol Biol. 26: 52-7.
  6. Ordway, D. et al. (2007) The cellular immune response to Mycobacterium tuberculosis infection in the guinea pig.
    J Immunol. 179: 2532-41.
  7. Ordway, D. et al. (2008) Influence of Mycobacterium bovis BCG vaccination on cellular immune response of guinea pigs challenged with Mycobacterium tuberculosis.
    Clin Vaccine Immunol. 15: 1248-58.