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CD1b3 antibody | MsGP9

Mouse anti Guinea Pig CD1b3

Product Type
Monoclonal Antibody
Clone
MsGP9
Isotype
IgG1
Specificity
CD1b3

Product Code Applications Pack Size List Price Your Price Qty
MCA566GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F 0.1 mg loader
List Price Your Price
loader

Mouse anti Guinea Pig CD1b3 antibody, clone MsGP9 recognizes an antigen expressed by guinea pig B lymphocytes.

The antigen is found on mature B cells, weakly on germinal centre B cells, and also on a proportion of thymocytes and peritoneal macrophages.

Mouse anti Guinea Pig CD1b3 antibody, clone MsGP9 appears to recognize the guinea pig homologue of CD1b3 (Hiromatsu et al. 2001).

Target Species
Guinea Pig
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Guinea pig thymocytes.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from an immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/200
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Source Reference

  1. Butter, C. et al. (1989) A quantitative immunocytochemical study of the infiltrating lymphocytes in the spinal cord of guinea pigs with chronic relapsing experimental allergic encephalomyelitis.
    J Neuroimmunol. 25 (2-3): 169-76.

References for CD1b3 antibody

  1. Meshi, B. et al. (2002) Emphysematous lung destruction by cigarette smoke. The effects of latent adenoviral infection on the lung inflammatory response.
    Am J Respir Cell Mol Biol. 26: 52-7.
  2. Dascher, C.C. et al. (2002) Conservation of CD1 intracellular trafficking patterns between mammalian species.
    J Immunol. 169: 6951-8.
  3. Hiromatsu, K. et al. (2002) Characterization of guinea-pig group 1 CD1 proteins.
    Immunology. 106 (2): 159-72.
  4. Ordway, D. et al. (2007) The cellular immune response to Mycobacterium tuberculosis infection in the guinea pig.
    J Immunol. 179: 2532-41.
  5. Ordway, D. et al. (2008) Influence of Mycobacterium bovis BCG vaccination on cellular immune response of guinea pigs challenged with Mycobacterium tuberculosis.
    Clin Vaccine Immunol. 15: 1248-58.
  6. Lanz, T.A. et al. (2010) Pharmacodynamics and pharmacokinetics of the gamma-secretase inhibitor PF-3084014.
    J Pharmacol Exp Ther. 334: 269-77.

Flow Cytometry

Immunohistology - Frozen

RRID
AB_11152940
UniProt
Q9QZZ0
Entrez Gene
Cd1b3
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005765 lysosomal membrane
GO:0006955 immune response
GO:0010008 endosome membrane
GO:0019882 antigen processing and presentation

MCA566GA

151032 164528 166198

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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