AMPK Alpha 1/2 antibody | 84.1
Mouse anti AMPK Alpha 1/2 antibody, clone 84.1 recognises both the alpha 1 and the alpha 2 isoforms of AMP-activated protein kinase (AMPK). AMPK is a highly conserved heterotrimeric protein kinase found in all eukaryotes. The heterotrimeric complex exists as multiple isoforms and consists of a catalytic alpha subunit, which is stabilised by non-catalytic regulatory beta- and gamma- subunits. AMPK plays a vital role in maintaining cellular energy homeostasis and is activated by rising levels of AMP under conditions of hypoxia, ischemia, exercise, low glucose levels.
AMPK may play an important role in the control of type 2 diabetes and metabolic syndromes. Recent studies suggest that AMPK functions as a suppressor of cell proliferation of both malignant and normal cells.
AMPK may play an important role in the control of type 2 diabetes and metabolic syndromes. Recent studies suggest that AMPK functions as a suppressor of cell proliferation of both malignant and normal cells.
Product Details
- Target Species
- Drosophila
- Species Cross-Reactivity
-
Target Species Cross Reactivity Vertebrates Expected from Sequence Human Expected from Sequence Mouse Expected from Sequence Rat Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Carrier Free
- Yes
- Immunogen
- 29 amino acid peptide within the N-terminal catalytic domain of Drosophila AMPK alpha.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
More Information
- UniProt
- Q13131
- Entrez Gene
- PRKAA1
- GO Terms
- GO:0000187 activation of MAPK activity
- GO:0005515 protein binding
- GO:0001666 response to hypoxia
- GO:0005829 cytosol
- GO:0004691 cAMP-dependent protein kinase activity
- GO:0046872 metal ion binding
- GO:0005524 ATP binding
- GO:0006633 fatty acid biosynthetic process
- GO:0006695 cholesterol biosynthetic process
- GO:0007050 cell cycle arrest
- GO:0008286 insulin receptor signaling pathway
- GO:0045542 positive regulation of cholesterol biosynthetic process
- GO:0045768 positive regulation of anti-apoptosis
- GO:0046318 negative regulation of glucosylceramide biosynthetic process
- GO:0046320 regulation of fatty acid oxidation
- Regulatory
- For research purposes only
Applications of AMPK Alpha 1/2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Immunoprecipitation | |||
| Western Blotting | 1/100 | 1/1000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- MCA2673GA detects a band of approximately 63kDa in rat brain cell lysates.
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