AMPK Alpha 1/2 antibody | 34.2

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Mouse anti AMPK Alpha 1/2

Product Type
Monoclonal Antibody
Clone
34.2
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2673GA C E IF IP WB 0.1 mg
Mouse anti AMPK Alpha 1/2 antibody, clone 34.2 recognises both the alpha 1 and the alpha 2 isoforms of AMP-activated protein kinase (AMPK). AMPK is a highly conserved heterotrimeric protein kinase found in all eukaryotes. The heterotrimeric complex exists as multiple isoforms and consists of a catalytic alpha subunit, which is stabilised by non-catalytic regulatory beta- and gamma- subunits. AMPK plays a vital role in maintaining cellular energy homeostasis and is activated by rising levels of AMP under conditions of hypoxia, ischemia, exercise, low glucose levels.

AMPK may play an important role in the control of type 2 diabetes and metabolic syndromes. Recent studies suggest that AMPK functions as a suppressor of cell proliferation of both malignant and normal cells.

Product Details

Target Species
Drosophila
Species Cross-Reactivity
Target SpeciesCross Reactivity
Vertebrates
Human
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
29 amino acid peptide within the N-terminal catalytic domain of Drosophila AMPK alpha.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P54646 Related reagents
Q13131 Related reagents
Entrez Gene
PRKAA2 Related reagents
PRKAA1 Related reagents
GO Terms
GO:0005829 cytosol
GO:0046872 metal ion binding
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0006112 energy reserve metabolic process
GO:0006468 protein phosphorylation
GO:0006633 fatty acid biosynthetic process
GO:0006695 cholesterol biosynthetic process
GO:0006853 carnitine shuttle
GO:0007050 cell cycle arrest
GO:0008286 insulin receptor signaling pathway
GO:0042304 regulation of fatty acid biosynthetic process
GO:0046320 regulation of fatty acid oxidation
GO:0000187 activation of MAPK activity
GO:0005515 protein binding
GO:0001666 response to hypoxia
GO:0004691 cAMP-dependent protein kinase activity
GO:0045542 positive regulation of cholesterol biosynthetic process
GO:0045768 positive regulation of anti-apoptosis
GO:0046318 negative regulation of glucosylceramide biosynthetic process
Regulatory
For research purposes only

Applications of AMPK Alpha 1/2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
Western Blotting 1/100 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
MCA2673GA detects a band of approximately 63kDa in rat brain cell lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F