CD20 antibody | NCD1.2
Mouse anti Dog CD20
- Product Type
- Monoclonal Antibody
- Clone
- NCD1.2
- Isotype
- IgG1
- Specificity
- CD20
Antibody Quality and Performance Guarantee
MCA6449GA
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| Mouse anti Dog CD20 antibody, clone NCD1.2 recognizes dog CD20. CD20 is an atypical tetraspanin expressed on B cells and is involved in B cell activation, proliferation, and differentiation. Similar to its expression in humans, canine CD20 is found on most mature B cells and is an important target for diagnosing and treating B-cell malignancies such as lymphoma. The extracellular domains of canine CD20 differ significantly in sequence and antigenicity from human CD20, which prevents most therapeutic anti human CD20 antibodies from binding to the canine protein. Mouse anti Dog CD20, clone NCD1.2, has been shown to detect CD20 on canine B cells, medium-sized B-cell lymphoma, and diffuse large B-cell lymphoma (DLBCL) (Jain et al. 2016). Studies using HDX-MS have also identified the dominant paratope in CD20 binding by NCD1.2, providing structural insights for antibody optimization for lymphoma treatment (Uhrik et al. 2021). |
- Target Species
- Dog
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- Peptide NCDPANPSEKNSLSIQYCGSIR from Canine CD20
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/100 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| Description | Mouse IgG1 Negative Control | ||||||
Antibody Characterization Reference
-
Jain, S. et al. (2016) The Development of a Recombinant scFv Monoclonal Antibody Targeting Canine CD20 for Use in Comparative Medicine.
PLoS One. 11
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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