CD18 antibody | CA1.4E9

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MCA1780A647
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F 100 Tests/1ml loader
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MCA1780GA
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C * F IF IP 0.1 mg loader
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Mouse anti Dog CD18 antibody, clone CA1.4E9 recognizes the canine CD18 cell surface antigen, also known as the b2 integrin.

The tissue and cellular distribution of CD18 in canine tissue closely follows that observed for humans (Moore et al. 1990) CD18 is expressed by virtually all leucocytes, but more strongly upon monocytes and granulocytes than on lymphocytes. The cross reactivity patterns of Mouse anti Canine CD18, clone CA1.4E9 further indicate that the antibody clone recognizes an epitope common to a number of mammalian species.

Immunoprecipitation experiments using detergent lysates of iodinated peripheral blood leukocytes indicate that clone Ca1.4E9 immunoprecipitates the common 95 kDa beta 2 integrin chain (CD18) along with the non-covalently associated alpha chains at 180 kDa (CD11a ), 165 kDa (CD11b) and 150 kDa (CD11c) (Moore et al. 1990). Of note is the lack of precipitation of the integrin alpha D chain (CD11d), this is likely due to the almost complete absence of CD11d expression on peripheral blood lymphocytes, in contrast to expression in the splenic red pulp (Fry et al. 2003).

High CD18 expression is a common feature of lymphoma and hyperplasia in dogs (Caniatti et al. 1996)

Target Species
Dog
Species Cross-Reactivity
Target SpeciesCross Reactivity
Horse
Pig
Human
Bovine
Cat
Mustelid Expected from Sequence
Mink
Hooded Seal
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/200
Immunofluorescence
Immunohistology - Frozen 1
Immunohistology - Paraffin
Immunoprecipitation
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood

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Description Mouse IgG1 Negative Control

References for CD18 antibody

  1. Moore, P.F. et al. (1990) Canine leukocyte integrins: characterization of a CD18 homologue.
    Tissue Antigens. 36 (5): 211-20.
  2. Danilenko, D.M. et al. (1992) Canine leukocyte cell adhesion molecules (leuCAMS): characterization of the CD11/CD18 family.
    Tissue Antigens 40: 13-21.
  3. Brodersen, R. et al. (1998) Analysis of the immunological cross reactivities of 213 well characterized monoclonal antibodies with specificities against various leucocyte surface antigens of human and 11 animal species.
    Vet Immunol Immunopathol. 64 (1): 1-13.
  4. Lecchi, C. et al. (2008) Bovine alpha-1 acid glycoprotein can reduce the chemotaxis of bovine monocytes and modulate CD18 expression.
    Vet Res. 39: 50.
  5. Bauer, T.R. Jr. et al. (2006) Correction of the disease phenotype in canine leukocyte adhesion deficiency using ex vivo hematopoietic stem cell gene therapy.
    Blood. 108: 3313-20.
  6. Leite, F. et al. (2000) Recombinant bovine interleukin-1beta amplifies the effects of partially purified Pasteurella haemolytica leukotoxin on bovine neutrophils in a beta(2)-integrin-dependent manner.
    Infect Immun. 68: 5581-6.
  7. McDonough, S.P. and Moore, P.F. (2000) Clinical, hematologic, and immunophenotypic characterization of canine large granular lymphocytosis
    Vet Pathol. 37: 637-46.
  8. Mortarino, M. et al. (2010) Identification of suitable endogenous controls and differentially expressed microRNAs in canine fresh-frozen and FFPE lymphoma samples.
    Leuk Res. 34: 1070-7.
  9. View The Latest Product References
  10. Donahue, R.E. et al. (2011) Leukocyte integrin activation mediates transient neutropenia after G-CSF administration.
    Blood. 118: 4209-14.
  11. Larsen, A.K. et al. (2013) Entry and Elimination of Marine Mammal Brucella spp. by Hooded Seal (Cystophora cristata) Alveolar Macrophages In Vitro.
    PLoS One. 8: e70186.
  12. Comazzi, S. et al. (2006) Flow cytometric expression of common antigens CD18/CD45 in blood from dogs with lymphoid malignancies: a semi-quantitative study.
    Vet Immunol Immunopathol. 112 (3-4): 243-52.
  13. Comazzi, S. et al. (2006) Flow cytometric patterns in blood from dogs with non-neoplastic and neoplastic hematologic diseases using double labeling for CD18 and CD45.
    Vet Clin Pathol. 35 (1): 47-54.
  14. Moreira, M.L. et al. (2016) Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes.
    Vet Parasitol. 220: 33-45.
  15. Bauer, T.R. Jr et al. (2017) Feline leukocyte adhesion (CD18) deficiency caused by a deletion in the integrin β2 (ITGB2) gene.
    Vet Clin Pathol. 46 (3): 391-400.
  16. Martini, V. et al. (2016) Canine small clear cell/T-zone lymphoma: clinical presentation and outcome in a retrospective case series.
    Vet Comp Oncol. 14 Suppl 1: 117-26.
  17. Brodzki, P. et al. (2019) Selected leukocyte subpopulations in peripheral blood and uterine washings in cows before and after intrauterine administration of cefapirin and methisoprinol.
    Anim Sci J. Nov 06 [Epub ahead of print].

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.

Flow Cytometry

Immunofluorescence

Synonyms
Integrin Beta 2 Chain
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