DNA-RNA Hybrid antibody | S9.6
Mouse anti DNA-RNA Hybrid
- Product Type
- Monoclonal Antibody
- Clone
- S9.6
- Isotype
- IgG2a
- Specificity
- DNA-RNA Hybrid
Antibody Quality and Performance Guarantee
| Mouse anti DNA-RNA hybrid antibody, clone S9.6 recognizes DNA-RNA hybrids. DNA-RNA hybrids, also known as R-loops occur naturally in eukaryotic cells as a result of non-covalant sequence-specific interaction between two or more complementary strands of nucleic acids into a single complex. Hybridization influences genomic instability and is particularly prevalent at sites which have high levels of transcriptional activity (Rich, 2006). Mouse anti DNA-RNA hybrid antibody can be used for the detection of DNA-RNA hybridization in microarray assays. |
- Target Species
- Broad
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.035% Sodium Azide (NaN3)
30% Glycerol - Carrier Free
- Yes
- Immunogen
- DNA-RNA hybrids synthesized from φX174 single stranded-DNA.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunoblotting |  |
1 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Source Reference
-
Boguslawski, S.J. et al. (1986) Characterization of monoclonal antibody to DNA.RNA and its application to immunodetection of hybrids.
J Immunol Methods. 89 (1): 123-30.
References for DNA-RNA Hybrid antibody
-
Gibbons, H.R. & Aune, T.M. (2020) Immunoprecipitation of DNA:RNA Hybrids Using the S9.6 Antibody.
Methods Mol Biol. 2161: 195-207. -
Smolka, J.A. et al. (2021) Recognition of RNA by the S9.6 antibody creates pervasive artifacts when imaging RNA:DNA hybrids.
J Cell Biol. 220 (6): e202004079.
Further Reading
-
Bou-Nader, C. et al. (2022) Structural basis of R-loop recognition by the S9.6 monoclonal antibody.
Nat Commun. 13 (1): 1641.
MCA6391
160680
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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