DNA-RNA Hybrid antibody | S9.6
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Mouse anti DNA-RNA Hybrid
- Product Type
- Monoclonal Antibody
- DNA-RNA Hybrid
|Mouse anti DNA-RNA hybrid antibody, clone S9.6 recognizes DNA-RNA hybrids.
DNA-RNA hybrids, also known as R-loops occur naturally in eukaryotic cells as a result of non-covalant sequence-specific interaction between two or more complementary strands of nucleic acids into a single complex. Hybridization influences genomic instability and is particularly prevalent at sites which have high levels of transcriptional activity (Rich, 2006).
Mouse anti DNA-RNA hybrid antibody can be used for the detection of DNA-RNA hybridization in microarray assays.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.035% Sodium Azide (NaN3)
- Carrier Free
- DNA-RNA hybrids synthesized from φX174 single stranded-DNA.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
|Application Name||Verified||Min Dilution||Max Dilution|
Boguslawski, S.J. et al. (1986) Characterization of monoclonal antibody to DNA.RNA and its application to immunodetection of hybrids.
J Immunol Methods. 89 (1): 123-30.
References for DNA-RNA Hybrid antibody
Gibbons, H.R. & Aune, T.M. (2020) Immunoprecipitation of DNA:RNA Hybrids Using the S9.6 Antibody.
Methods Mol Biol. 2161: 195-207.
Smolka, J.A. et al. (2021) Recognition of RNA by the S9.6 antibody creates pervasive artifacts when imaging RNA:DNA hybrids.
J Cell Biol. 220 (6): e202004079.
Bou-Nader, C. et al. (2022) Structural basis of R-loop recognition by the S9.6 monoclonal antibody.
Nat Commun. 13 (1): 1641.
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