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WC1 antibody | CC15

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Anti Bovine WC1 Antibody, clone CC15 thumbnail image 1 Anti Bovine WC1 Antibody, clone CC15 thumbnail image 2 Anti Bovine WC1 Antibody, clone CC15 thumbnail image 3
Anti Bovine WC1 Antibody, clone CC15 gallery image 1
Figure A. RPE conjugated Mouse anti Bovine CD45RO Antibody (MCA2434PE) and FITC conjugated Mouse IgG2a isotype control (MCA929F). Figure B. RPE conjugated Mouse anti Bovine CD45RO Antibody (MCA2434PE) and FITC conjugated Mouse anti Bovine WC1 Antibody (MCA838F). All experiments performed on red cell lysed bovine blood gated on lymphoid cells in the presence of 10% rat serum. Data acquired on the ZE5™ Cell Analyzer.
Anti Bovine WC1 Antibody, clone CC15 gallery image 2
Published customer image:
Mouse anti Bovine WC1 antibody, clone CC15 (MCA838) used to identify WC1 expressing lymphocytes in tick infested bovine skin by immunohistochemistry on formalin fixed paraffin embedded tissue sections.
Image caption:
Lymphocyte phenotypes in tick-infested skins. Cryopreserved sections were stained with peroxidase-immunohistochemistry to counts local lymphocytes, as described in the methods section. The blue arrowhead marks cement cone produced by R. microplus showing the sections in the center of tick attachment (Zone 1 or 2, Additional file 1: Figure S1b), while black arrowhead marks the lymphocytes surrounding cement cone (original magnifications were 10× and 100×).

From: Franzin AM, Maruyama SR, Garcia GR, Oliveira RP, Ribeiro JM, Bishop R, Maia AA, Moré DD, Ferreira BR, Santos IK.
Immune and biochemical responses in skin differ between bovine hosts genetically susceptible and resistant to the cattle tick Rhipicephalus microplus. Parasit Vectors. 2017 Jan 31;10(1):51.
Anti Bovine WC1 Antibody, clone CC15 gallery image 3
Published customer image:
FITC conjugated Mouse anti Bovine WC1 antibody, clone CC15 (MCA838F) used for the evaluation of WC1 expression on ovine peritoneal cells by flow cytometry.
Image caption:
Percentage of cell populations in the peritoneal fluid during the early stages of infection. The specific cell populations are shown as follows: (a) TCD4; (b) TCD8; (c) WC1+γδ; (d) CD14; (e) MHCII; (f) CD83. Cell subsets of uninfected (Neg. Ctl) and infected sheep (Group 1) are shown in columns. Mann-Whitney U-test was used to compare data from Groups 1 and 3. Values represent the mean ± standard deviation (SD). Asterisks indicate different levels of significance between groups: *P ≤ 0.05, **P ≤ 0.01

From: Pérez-Caballero R, Javier Martínez-Moreno F, Zafra R, Molina-Hernández V, Pacheco IL, Teresa Ruiz-Campillo M, Escamilla A, Pérez J, Martnez-Moreno Á, Buffoni L.
Comparative dynamics of peritoneal cell immunophenotypes in sheep during the early and late stages of the infection with Fasciola hepatica by flow cytometric analysis.
Parasit Vectors. 2018 11(1):640.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Bovine WC1:FITC

Mouse anti Bovine WC1

Product Type
Monoclonal Antibody
Clone
CC15
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA838F
Datasheet
F
SDS
0.1 mg loader

MCA838GA
Datasheet
C F IP P*
SDS
0.1 mg loader

Mouse anti Bovine WC1 antibody, clone CC15 recognizes bovine WC1, a ~215/300 kDa antigen expressed on the majority of gamma/delta T lymphocytes. These cells also express low levels of CD5, but are negative for other B and T cell markers.

The WC1 antigen is reported to be involved in a number of processes, including activation of gamma/delta T cells and the development of a Th1-biased acquired immune response (Rogers et al. 2005).

Mouse anti Bovine WC1 antibody, clone CC15 is routinely tested in flow cytometry on bovine peripheral blood lymphocytes.

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Sheep
Goat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Bovine lymphocytes
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P30205
Entrez Gene
CD163L1
GO Terms
GO:0016020 membrane
GO:0005576 extracellular region
GO:0005044 scavenger receptor activity
Regulatory
For research purposes only

Applications of WC1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry 1/25 1/200
Immunohistology - Frozen
Immunohistology - Paraffin 1
Immunoprecipitation
  1. 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase. Overnight incubation with clone CC15 is recommended.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
Human anti Mouse IgG2a:FITC HCA037F F 0.1 mg loader
Human anti Mouse IgG2a:HRP HCA037P E 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG2a Negative Control:FITC MCA929F F 100 Tests loader
Mouse IgG2a Negative Control MCA929 F 100 Tests loader

Application Based External Images

Flow Cytometry

Product Specific References

References for WC1 antibody

  1. Howard, C.J. et al. (1989) In vivo depletion of BoT4 (CD4) and of non-T4/T8 lymphocyte subsets in cattle with monoclonal antibodies.
    Eur J Immunol. 19 (4): 757-64.
  2. Clevers, H. et al. (1990) Identification of a bovine surface antigen uniquely expressed on CD4-CD8- T cell receptor gamma/delta+ T lymphocytes.
    Eur J Immunol. 20 (4): 809-17.
  3. Gutierrez, M. et al. (1999) The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques.
    Vet Immunol Immunopathol. 71 (3-4): 321-34.
  4. Brodersen, R. et al. (1998) Analysis of the immunological cross reactivities of 213 well characterized monoclonal antibodies with specificities against various leucocyte surface antigens of human and 11 animal species.
    Vet Immunol Immunopathol. 64 (1): 1-13.
  5. Winkler, M.T. et al. (2000) Persistence and reactivation of bovine herpesvirus 1 in the tonsils of latently infected calves.
    J Virol. 74 (11): 5337-46.
  6. Sanchez, J. et al. (2011) Microscopical and immunological features of tuberculoid granulomata and cavitary pulmonary tuberculosis in naturally infected goats.
    J Comp Pathol. 145 (2-3): 107-17.
  7. Fulton, B.E. Jr. et al. (2006) Dissemination of bovine leukemia virus-infected cells from a newly infected sheep lymph node.
    J Virol. 80: 7873-84.
  8. Glew, E.J. and Howard, C.J. (2001) Antigen-presenting cells from calves persistently infected with bovine viral diarrhoea virus, a member of the Flaviviridae, are not compromised in their ability to present viral antigen.
    J Gen Virol. 82: 1677-85.
  9. Liebana, E. et al. (2007) Distribution and activation of T-lymphocyte subsets in tuberculous bovine lymph-node granulomas.
    Vet Pathol. 44: 366-72.
  10. Lynch, E.M. et al. (2010) Effect of abrupt weaning at housing on leukocyte distribution, functional activity of neutrophils, and acute phase protein response of beef calves.
    BMC Vet Res. 6: 39.
  11. Summers, C. et al. (2012) The distribution of immune cells in the lungs of classical and atypical ovine pulmonary adenocarcinoma.
    Vet Immunol Immunopathol. 146: 1-7.
  12. Maślanka T et al. (2012) The presence of CD25 on bovine WC1+ γ/δ T cells is positively correlated with their production of IL-10 and TGF-β, but not IFN-γ.
    Pol J Vet Sci. 15 (1): 11-20.
  13. Herzig, C.T. et al. (2010) Evolution of the CD163 family and its relationship to the bovine gamma delta T cell co-receptor WC1.
    BMC Evol Biol. 10: 181.
  14. Romero-Palomo F et al. (2015) Immunopathologic Changes in the Thymus of Calves Pre-infected with BVDV and Challenged with BHV-1.
    Transbound Emerg Dis. Aug 25. [Epub ahead of print]
  15. Goh, S. et al. (2016) Identification of Theileria lestoquardi Antigens Recognized by CD8+ T Cells.
    PLoS One. 11 (9): e0162571.
  16. Wattegedera, S.R. et al. (2017) Enhancing the toolbox to study IL-17A in cattle and sheep.
    Vet Res. 48 (1): 20.
  17. Franzin, A.M. et al. (2017) Immune and biochemical responses in skin differ between bovine hosts genetically susceptible and resistant to the cattle tick Rhipicephalus microplus.
    Parasit Vectors. 10 (1): 51.
  18. Palomares, R.A. et al. (2015) Acute infection with bovine viral diarrhea virus of low or high virulence leads to depletion and redistribution of WC1(+) γδ T cells in lymphoid tissues of beef calves.
    Vet Immunol Immunopathol. 167 (3-4): 190-5.
  19. Blumerman SL et al. (2007) Molecular cloning of bovine chemokine receptors and expression by WC1+ γδ T cells.
    Dev Comp Immunol. 31 (1): 87-102.
  20. Elsayed, M. et al. (2016) Comparison between Immunological and Molecular Based Methods for Diagnosis of Mycobacterium Infections in Cattle, Buffaloes and Human in Egypt.
    Cell Mol Biol 62: 125.
  21. Hecker YP et al. (2013) Immune response and protection provided by live tachyzoites and native antigens from the NC-6 Argentina strain of Neospora caninum in pregnant heifers.
    Vet Parasitol. 197 (3-4): 436-46.
  22. Martínez CM et al. (2005) Immunophenotypical characterization of lymphocyte subpopulations of the uterus of non-pregnant and pregnant goats.
    Anat Histol Embryol. 34 (4): 240-6.
  23. Silva, A.P. et al. (2015) Encapsulated Brucella ovis Lacking a Putative ATP-Binding Cassette Transporter (ΔabcBA) Protects against Wild Type Brucella ovis in Rams.
    PLoS One. 10 (8): e0136865.
  24. Albertsson, A.M. et al. (2018) γδ T Cells Contribute to Injury in the Developing Brain.
    Am J Pathol. 188 (3): 757-67.
  25. Higgins, J.L. et al. (2018) Cell mediated immune response in goats after experimental challenge with the virulent Brucella melitensis strain 16M and the reduced virulence strain Rev. 1.
    Vet Immunol Immunopathol. 202: 74-84.
  26. Baliu-piqué, M. et al. (2019) Age-related distribution and dynamics of T-cells in blood and lymphoid tissues of goats.
    Dev Comp Immunol. 93: 1-10.
  27. Pérez-caballero, R. et al. (2018) Comparative dynamics of peritoneal cell immunophenotypes in sheep during the early and late stages of the infection with Fasciola hepatica by flow cytometric analysis.
    Parasit Vectors. 11 (1): 640.
  28. Gondaira, S. et al. (2020) Immunosuppression in Cows following Intramammary Infusion of Mycoplasma bovis.
    Infect Immun. 88 (3)Feb 20 [Epub ahead of print].

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