WC1 antibody | CC15



The WC1 antigen is reported to be involved in a number of processes, including activation of gamma/delta T cells and the development of a Th1-biased acquired immune response (Rogers et al. 2005).
Mouse anti Bovine WC1 antibody, clone CC15 is routinely tested in flow cytometry on bovine peripheral blood lymphocytes.
Product Details
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Sheep Goat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Bovine lymphocytes
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P30205
- Entrez Gene
- CD163L1
- GO Terms
- GO:0016020 membrane
- GO:0005576 extracellular region
- GO:0005044 scavenger receptor activity
- Regulatory
- For research purposes only
Applications of WC1 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | 1/10 | |
Flow Cytometry | 1/25 | 1/200 | |
Immunohistology - Frozen | |||
Immunohistology - Paraffin 1 | |||
Immunoprecipitation |
- 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase. Overnight incubation with clone CC15 is recommended.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG2a Negative Control:FITC | MCA929F | F | 100 Tests |
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Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
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Product Specific References
References for WC1 antibody
-
Howard, C.J. et al. (1989) In vivo depletion of BoT4 (CD4) and of non-T4/T8 lymphocyte subsets in cattle with monoclonal antibodies.
Eur J Immunol. 19 (4): 757-64. -
Clevers, H. et al. (1990) Identification of a bovine surface antigen uniquely expressed on CD4-CD8- T cell receptor gamma/delta+ T lymphocytes.
Eur J Immunol. 20 (4): 809-17. -
Gutierrez, M. et al. (1999) The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques.
Vet Immunol Immunopathol. 71 (3-4): 321-34. -
Brodersen, R. et al. (1998) Analysis of the immunological cross reactivities of 213 well characterized monoclonal antibodies with specificities against various leucocyte surface antigens of human and 11 animal species.
Vet Immunol Immunopathol. 64 (1): 1-13. -
Winkler, M.T. et al. (2000) Persistence and reactivation of bovine herpesvirus 1 in the tonsils of latently infected calves.
J Virol. 74 (11): 5337-46. -
Sanchez, J. et al. (2011) Microscopical and immunological features of tuberculoid granulomata and cavitary pulmonary tuberculosis in naturally infected goats.
J Comp Pathol. 145 (2-3): 107-17. -
Fulton, B.E. Jr. et al. (2006) Dissemination of bovine leukemia virus-infected cells from a newly infected sheep lymph node.
J Virol. 80: 7873-84. -
Glew, E.J. and Howard, C.J. (2001) Antigen-presenting cells from calves persistently infected with bovine viral diarrhoea virus, a member of the Flaviviridae, are not compromised in their ability to present viral antigen.
J Gen Virol. 82: 1677-85. -
Liebana, E. et al. (2007) Distribution and activation of T-lymphocyte subsets in tuberculous bovine lymph-node granulomas.
Vet Pathol. 44: 366-72. -
Lynch, E.M. et al. (2010) Effect of abrupt weaning at housing on leukocyte distribution, functional activity of neutrophils, and acute phase protein response of beef calves.
BMC Vet Res. 6: 39. -
Summers, C. et al. (2012) The distribution of immune cells in the lungs of classical and atypical ovine pulmonary adenocarcinoma.
Vet Immunol Immunopathol. 146: 1-7. -
Maślanka T et al. (2012) The presence of CD25 on bovine WC1+ γ/δ T cells is positively correlated with their production of IL-10 and TGF-β, but not IFN-γ.
Pol J Vet Sci. 15 (1): 11-20. -
Herzig, C.T. et al. (2010) Evolution of the CD163 family and its relationship to the bovine gamma delta T cell co-receptor WC1.
BMC Evol Biol. 10: 181. -
Romero-Palomo F et al. (2015) Immunopathologic Changes in the Thymus of Calves Pre-infected with BVDV and Challenged with BHV-1.
Transbound Emerg Dis. Aug 25. [Epub ahead of print] -
Goh, S. et al. (2016) Identification of Theileria lestoquardi Antigens Recognized by CD8+ T Cells.
PLoS One. 11 (9): e0162571. -
Wattegedera, S.R. et al. (2017) Enhancing the toolbox to study IL-17A in cattle and sheep.
Vet Res. 48 (1): 20. -
Franzin, A.M. et al. (2017) Immune and biochemical responses in skin differ between bovine hosts genetically susceptible and resistant to the cattle tick Rhipicephalus microplus.
Parasit Vectors. 10 (1): 51. -
Palomares, R.A. et al. (2015) Acute infection with bovine viral diarrhea virus of low or high virulence leads to depletion and redistribution of WC1(+) γδ T cells in lymphoid tissues of beef calves.
Vet Immunol Immunopathol. 167 (3-4): 190-5. -
Blumerman SL et al. (2007) Molecular cloning of bovine chemokine receptors and expression by WC1+ γδ T cells.
Dev Comp Immunol. 31 (1): 87-102. -
Elsayed, M. et al. (2016) Comparison between Immunological and Molecular Based Methods for Diagnosis of Mycobacterium Infections in Cattle, Buffaloes and Human in Egypt.
Cell Mol Biol 62: 125. -
Hecker YP et al. (2013) Immune response and protection provided by live tachyzoites and native antigens from the NC-6 Argentina strain of Neospora caninum in pregnant heifers.
Vet Parasitol. 197 (3-4): 436-46. -
Martínez CM et al. (2005) Immunophenotypical characterization of lymphocyte subpopulations of the uterus of non-pregnant and pregnant goats.
Anat Histol Embryol. 34 (4): 240-6. -
Silva, A.P. et al. (2015) Encapsulated Brucella ovis Lacking a Putative ATP-Binding Cassette Transporter (ΔabcBA) Protects against Wild Type Brucella ovis in Rams.
PLoS One. 10 (8): e0136865. -
Albertsson, A.M. et al. (2018) γδ T Cells Contribute to Injury in the Developing Brain.
Am J Pathol. 188 (3): 757-67. -
Higgins, J.L. et al. (2018) Cell mediated immune response in goats after experimental challenge with the virulent Brucella melitensis strain 16M and the reduced virulence strain Rev. 1.
Vet Immunol Immunopathol. 202: 74-84. -
Baliu-piqué, M. et al. (2019) Age-related distribution and dynamics of T-cells in blood and lymphoid tissues of goats.
Dev Comp Immunol. 93: 1-10. -
Pérez-caballero, R. et al. (2018) Comparative dynamics of peritoneal cell immunophenotypes in sheep during the early and late stages of the infection with Fasciola hepatica by flow cytometric analysis.
Parasit Vectors. 11 (1): 640. -
Gondaira, S. et al. (2020) Immunosuppression in Cows following Intramammary Infusion of Mycoplasma bovis.
Infect Immun. 88 (3)Feb 20 [Epub ahead of print].
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