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CD45RO antibody | IL-A116

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Anti Bovine CD45RO Antibody, clone IL-A116 thumbnail image 1 Anti Bovine CD45RO Antibody, clone IL-A116 thumbnail image 2 Anti Bovine CD45RO Antibody, clone IL-A116 thumbnail image 3 Anti Bovine CD45RO Antibody, clone IL-A116 thumbnail image 4 Anti Bovine CD45RO Antibody, clone IL-A116 thumbnail image 5
Anti Bovine CD45RO Antibody, clone IL-A116 gallery image 1
Figure A. FITC conjugated Mouse anti Bovine CD4 (MCA1653F) and RPE conjugated Mouse IgG3 isotype control (MCA5920PE). Figure B. FITC conjugated Mouse anti Bovine CD4 (MCA1653F) and RPE conjugated Mouse anti Bovine CD45RO (MCA2434PE). All experiments performed on red cell lysed bovine blood gated on lymphoid cells in the presence of 10% rat serum. Data acquired on the ZE5 Cell Analyzer.
Anti Bovine CD45RO Antibody, clone IL-A116 gallery image 2
Figure A. RPE-Cy5 conjugated Mouse anti Bovine CD172a (MCA2041C) and RPE conjugated Mouse IgG3 isotype control (MCA5920PE). Figure B. RPE-Cy5 conjugated Mouse anti Bovine CD172a (MCA2041C) and RPE conjugated Mouse anti Bovine CD45RO (MCA2434PE). All experiments performed on red cell lysed bovine blood gated on lymphoid cells in the presence of 10% rat serum. Data acquired on the ZE5 Cell Analyzer.
Anti Bovine CD45RO Antibody, clone IL-A116 gallery image 3
Figure A. FITC conjugated Mouse anti Bovine WC1 (MCA838F) and RPE conjugated Mouse IgG3 isotype control (MCA5920PE). Figure B. FITC conjugated Mouse anti Bovine WC1(MCA838F) and RPE conjugated Mouse anti Bovine CD45RO(MCA2434PE). All experiments performed on red cell lysed bovine blood gated on lymphoid cells in the presence of 10% rat serum. Data acquired on the ZE5 Cell Analyzer.
Anti Bovine CD45RO Antibody, clone IL-A116 gallery image 4
Figure A. RPE-Cy5 conjugated Mouse anti Bovine CD172a (MCA2041C) and RPE conjugated Mouse IgG3 isotype control (MCA5920PE). Figure B. RPE-Cy5 conjugated Mouse anti Bovine CD172a (MCA2041C) and Mouse anti Bovine CD45RO (MCA2434GA) conjugated with Human anti Mouse IgG3:RPE antibody. All experiments performed on red cell lysed bovine blood gated on lymphoid cells in the presence of 10% rat serum. Data acquired on the ZE5 Cell Analyzer.
Anti Bovine CD45RO Antibody, clone IL-A116 gallery image 5
Published customer image:
PE conjugated Mouse anti Bovine CD45RO antibody, clone IL-A166 (MCA2434PE) used to evaluate CD45RO expression on caprine PBMC by flow cytometry.
Image caption:
Gating strategy for the determination of frequency of the IFN-γ positive lymphocyte subsets. Peripheral blood mononuclear cells (PBMC) from all goats were cultured in the presence of M. bovis tuberculin (PPD-B). (A,B) Singlet lymphocytes were identified based on the degree of cellular differentiation determined by forward scatter (FSC) and side scatter (SCC). (C) Representative frequencies of the CD4/CD45RO cell populations. (D) Representative frequencies of intracellular IFN-γ staining of cells gated from prelabelled CD4+CD45RO+.

From: Arrieta-Villegas C, Vidal E, Martín M, Verdés J, Moll X, Espada Y, Singh M, Villarreal-Ramos B, Domingo M, Pérez de Val B.
Immunogenicity and Protection against Mycobacterium caprae. Challenge in Goats Vaccinated with BCG and Revaccinated after One Year.
Vaccines (Basel). 2020 Dec 10;8(4):751.
doi: 10.3390/vaccines8040751..
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Mouse anti Bovine CD45RO:RPE

Product Type
Monoclonal Antibody
Clone
IL-A116
Isotype
IgG3
Specificity
CD45RO

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Mouse anti Bovine CD45RO, clone IL-A116 recognizes the bovine homologue of the human CD45RO cell surface antigen.

CD45, also known as Leucocyte Common Antigen or LCA, occurs in a number of isoforms, clone IL-A116 is specific for the low molecular weight isoform termed CD45RO, the isoform associated with expression on memory T-cells. Bovine CD45RO is expressed by monocytes, granulocytes and subsets of thymocytes, CD4+ T cells and CD8+ T cells. CD45RO+ CD8+ T cells increase from approximately 5% in neonatal calves to approximately 35% in adult cattle over the age of 5 years (Hogg et al. 2011). Mouse anti Bovine CD45RO, clone IL-A116 immunoprecipitates a molecule of ~180 kDa (Bembridge et al. 1995), analogus to the molecular weight of human and mouse CD45RO.

Mouse anti Bovine CD45RO, clone IL-A116 recognizes the CD45RO cell surface antigen by flow cytometry in both European cattle, Bos taurus, and in Zebu, B.indicus (Bembridge et al. 1995).

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Goat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Immunogen
Bovine peripheral blood monouclear cells.
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC.
Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

How to Use the Spectraviewer

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  • Select combined or multi-laser view to visualize the spectra

References for CD45RO antibody

  1. Howard, C.J. & Naessens, J. (1993) Summary of workshop findings for cattle (tables 1 and 2).
    Vet Immunol Immunopathol. 39 (1-3): 25-47.
  2. Naessens, J. et al. (1993) Cross-reactivity of workshop antibodies with cells from domestic and wild ruminants.
    Vet Immunol Immunopathol. 39 (1-3): 283-90.
  3. Bembridge, G.P. et al. (1993) Comparison of monoclonal antibodies with potential specificity for restricted isoforms of the leukocyte common antigen (CD45R).
    Vet Immunol Immunopathol. 39 (1-3): 129-36.
  4. Bembridge, G.P. et al. (1995) CD45RO expression on bovine T cells: relation to biological function.
    Immunology. 86 (4): 537-44.
  5. Mcinnes, E. et al. (1999) Phenotypic analysis of local cellular responses in calves infected with bovine respiratory syncytial virus.
    Immunology. 96 (3): 396-403.
  6. Sopp, P. & Howard, C.J. (2001) IFN gamma and IL-4 production by CD4, CD8 and WC1 gamma delta TCR(+) T cells from cattle lymph nodes and blood.
    Vet Immunol Immunopathol. 81 (1-2): 85-96.
  7. Hogg, A.E. et al. (2011) Characterization of age-related changes in bovine CD8+ T-cells.
    Vet Immunol Immunopathol. 140 (1-2): 47-54.
  8. Whelan, A.O. et al. (2011) Development of an antibody to bovine IL-2 reveals multifunctional CD4 T(EM) cells in cattle naturally infected with bovine tuberculosis.
    PLoS One. 6 (12): e29194.
    9. View The Latest Product References
  9. Schmidt, N. et al. (2018) Decreased STEC shedding by cattle following passive and active vaccination based on recombinant Escherichia coli Shiga toxoids.
    Vet Res. 49 (1): 28.
  10. Arrieta-Villegas, C. et al. (2020) Immunogenicity and Protection against Mycobacterium caprae Challenge in Goats Vaccinated with BCG and Revaccinated after One Year.
    Vaccines (Basel). 8 (4): 751.
  11. Wherry, T.L.T. et al. (2022) Effects of 1,25-Dihydroxyvitamin D3 and 25-Hydroxyvitamin D3 on PBMCs From Dairy Cattle Naturally Infected With Mycobacterium avium subsp. paratuberculosis.
    Front Vet Sci. 9: 830144.
  12. Hidalgo-Ruiz, M. et al. (2022) Babesia bovis AMA-1, MSA-2c and RAP-1 contain conserved B and T-cell epitopes, which generate neutralizing antibodies and a long-lasting Th1 immune response in vaccinated cattle.
    Vaccine. S0264-410X(22)00049-4.

MCA2434PE

INN1608

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