Ubiquitin antibody | 1B4-UB

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
Flow Cytometry 1		1/10	1/50
Immunohistology - Frozen		1/500	1/1000
Immunohistology - Paraffin		1/500	1/1000
Western Blotting		1/100	1/1000

1. ¹ Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Histology Positive Control Tissue

Human hypophysis

References for Ubiquitin antibody

1. (2011) Human Protein Atlas: CAB000362
   Human Protein Atlas: CAB000362
2. Sun, L. et al. (1999) Molecular identification of a murine ubiquitin/60S ribosomal fusion protein and expression study in mouse kidney.
   Biochem Genet. 37: 139-47.
3. Chen, W.Y. et al. (2007) Histone deacetylase inhibitors reduce steroidogenesis through SCF-mediated ubiquitination and degradation of steroidogenic factor 1 (NR5A1).
   Mol Cell Biol. 27: 7284-90.
4. Bista, M. et al. (2013) MDMX contains an autoinhibitory sequence element.
   Proc Natl Acad Sci U S A. 110: 17814-9.
5. Ma, L. et al. (2019) Composition of the Intranuclear Inclusions of Fragile X-associated Tremor/Ataxia Syndrome.
   Acta Neuropathol Commun. 7 (1): 143.

Immunohistology - Paraffin

Western Blotting

RRID

AB_2180567

UniProt

P62990

MCA1398G

0310 156232 161444

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