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Ubiquitin antibody | 1B4-UB

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Anti Ubiquitin Antibody, clone 1B4-UB thumbnail image 1 Anti Ubiquitin Antibody, clone 1B4-UB thumbnail image 2 Anti Ubiquitin Antibody, clone 1B4-UB thumbnail image 3
Anti Ubiquitin Antibody, clone 1B4-UB gallery image 1
Staining of paraffin embedded human tonsil with Mouse anti Ubiquitin (MCA1398G).
Anti Ubiquitin Antibody, clone 1B4-UB gallery image 2
Staining of human hypophysis. Formalin fixed, paraffin processed tissue with Mouse anti Ubiquitin (MCA1398G).
Anti Ubiquitin Antibody, clone 1B4-UB gallery image 3
GST-ubiquitin detected with Mouse anti Ubiquitin (MCA1398G).

Mouse anti Ubiquitin

Product Type
Monoclonal Antibody
Clone
1B4-UB
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA1398G
Datasheet
C E F* P WB
MSDS
0.1 mg loader

Mouse anti Ubiquitin antibody, clone 1B4-UB recognizes both human and bovine ubiquitin.

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Ubiquitin from bovine erythrocytes conjugated to bovine gamma globulin.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mouse were fused with cells of the mouse PAI myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P62990
Regulatory
For research purposes only

Applications of Ubiquitin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1 1/10 1/50
Immunohistology - Frozen 1/500 1/1000
Immunohistology - Paraffin 1/500 1/1000
Western Blotting 1/100 1/1000
  1. 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Immunohistology
This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Histology Positive Control Tissue
Human hypophysis
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader

Application Based External Images

Immunohistology - Paraffin

Western Blotting

Product Specific References

References for Ubiquitin antibody

  1. Sun, L. et al. (1999) Molecular identification of a murine ubiquitin/60S ribosomal fusion protein and expression study in mouse kidney.
    Biochem Genet. 37: 139-47.
  2. Chen, W.Y. et al. (2007) Histone deacetylase inhibitors reduce steroidogenesis through SCF-mediated ubiquitination and degradation of steroidogenic factor 1 (NR5A1).
    Mol Cell Biol. 27: 7284-90.
  3. Bista, M. et al. (2013) MDMX contains an autoinhibitory sequence element.
    Proc Natl Acad Sci U S A. 110: 17814-9.
  4. Ma, L. et al. (2019) Composition of the Intranuclear Inclusions of Fragile X-associated Tremor/Ataxia Syndrome.
    Acta Neuropathol Commun. 7 (1): 143.
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