Ubiquitin antibody | 1B4-UB
Mouse anti Ubiquitin antibody, clone 1B4-UB recognizes both human and bovine ubiquitin.
Product Details
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Ubiquitin from bovine erythrocytes conjugated to bovine gamma globulin.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mouse were fused with cells of the mouse PAI myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P62990
- Regulatory
- For research purposes only
Applications of Ubiquitin antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Flow Cytometry 1 | 1/10 | 1/50 | |
| Immunohistology - Frozen | 1/500 | 1/1000 | |
| Immunohistology - Paraffin | 1/500 | 1/1000 | |
| Western Blotting | 1/100 | 1/1000 |
- 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Immunohistology
- This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
- Histology Positive Control Tissue
- Human hypophysis
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Product Specific References
References for Ubiquitin antibody
-
Sun, L. et al. (1999) Molecular identification of a murine ubiquitin/60S ribosomal fusion protein and expression study in mouse kidney.
Biochem Genet. 37: 139-47. -
Chen, W.Y. et al. (2007) Histone deacetylase inhibitors reduce steroidogenesis through SCF-mediated ubiquitination and degradation of steroidogenic factor 1 (NR5A1).
Mol Cell Biol. 27: 7284-90. -
Bista, M. et al. (2013) MDMX contains an autoinhibitory sequence element.
Proc Natl Acad Sci U S A. 110: 17814-9. -
Ma, L. et al. (2019) Composition of the Intranuclear Inclusions of Fragile X-associated Tremor/Ataxia Syndrome.
Acta Neuropathol Commun. 7 (1): 143.
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