Mouse anti Ubiquitin antibody, clone 1B4-UB recognizes both human and bovine ubiquitin.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Carrier Free
- Ubiquitin from bovine erythrocytes conjugated to bovine gamma globulin.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mouse were fused with cells of the mouse PAI myeloma cell line.
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Ubiquitin antibody
|Flow Cytometry 1
|Immunohistology - Frozen
|Immunohistology - Paraffin
- 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
- Histology Positive Control Tissue
- Human hypophysis
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
Application Based External Images
Immunohistology - Paraffin
Product Specific References
References for Ubiquitin antibody
(2011) Human Protein Atlas: CAB000362
Human Protein Atlas: CAB000362
Sun, L. et al. (1999) Molecular identification of a murine ubiquitin/60S ribosomal fusion protein and expression study in mouse kidney.
Biochem Genet. 37: 139-47.
Chen, W.Y. et al. (2007) Histone deacetylase inhibitors reduce steroidogenesis through SCF-mediated ubiquitination and degradation of steroidogenic factor 1 (NR5A1).
Mol Cell Biol. 27: 7284-90.
Bista, M. et al. (2013) MDMX contains an autoinhibitory sequence element.
Proc Natl Acad Sci U S A. 110: 17814-9.
Ma, L. et al. (2019) Composition of the Intranuclear Inclusions of Fragile X-associated Tremor/Ataxia Syndrome.
Acta Neuropathol Commun. 7 (1): 143.
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