Ubiquitin antibody | 1B4-UB

Mouse anti Ubiquitin

Product Type
Monoclonal Antibody
Clone
1B4-UB
Isotype
IgG1
Specificity
Ubiquitin

Product Code Applications Pack Size List Price Your Price Qty
MCA1398G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E F * P WB 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Ubiquitin antibody, clone 1B4-UB recognizes both human and bovine ubiquitin.

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Ubiquitin from bovine erythrocytes conjugated to bovine gamma globulin.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mouse were fused with cells of the mouse PAI myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1 1/10 1/50
Immunohistology - Frozen 1/500 1/1000
Immunohistology - Paraffin 1/500 1/1000
Western Blotting 1/100 1/1000
  1. 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Immunohistology
This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Histology Positive Control Tissue
Human hypophysis

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Description Mouse IgG1 Negative Control

References for Ubiquitin antibody

  1. (2011) Human Protein Atlas: CAB000362
    Human Protein Atlas: CAB000362
  2. Sun, L. et al. (1999) Molecular identification of a murine ubiquitin/60S ribosomal fusion protein and expression study in mouse kidney.
    Biochem Genet. 37: 139-47.
  3. Chen, W.Y. et al. (2007) Histone deacetylase inhibitors reduce steroidogenesis through SCF-mediated ubiquitination and degradation of steroidogenic factor 1 (NR5A1).
    Mol Cell Biol. 27: 7284-90.
  4. Bista, M. et al. (2013) MDMX contains an autoinhibitory sequence element.
    Proc Natl Acad Sci U S A. 110: 17814-9.
  5. Ma, L. et al. (2019) Composition of the Intranuclear Inclusions of Fragile X-associated Tremor/Ataxia Syndrome.
    Acta Neuropathol Commun. 7 (1): 143.

Immunohistology - Paraffin

Western Blotting

RRID
AB_2180567
UniProt
P62990
View more products with UBIQUITIN specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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