MBP antibody | 1
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Mouse anti Bovine MBP (aa129-138)
- Product Type
- Monoclonal Antibody
- Clone
- 1
- Isotype
- IgG2a
- Specificity
- MBP
- Region
- (aa129-138)
Antibody Quality and Performance Guarantee
| Mouse anti Bovine MBP (aa129-138) antibody, clone 1 recognizes myelin basic protein (MBP). Clone 1 is reactive with an epitope in the 129-138 region of the human MBP molecule. The numbering of MBP residues is that as described by Martenson, R. E., 1984. Clone 1 (129-138) has been reported as being suitable for Western blotting. |
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Tissue Culture Supernatant - liquid
- Preparation
- Tissue Culture Supernatant containing 0.1M Tris/HCl pH7.4 and 5-10% foetal calf serum
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Bovine myelin basic protein (MBP).
- Fusion Partners
- Spleen cells from immunized NIH mice were fused with cells of the NS0 mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/200 | 1/1000 |
| Immunohistology - Frozen | |
1/10 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Source Reference
-
Groome, N. et al. (1985) Preparation and properties of monoclonal antibodies to myelin basic protein and its peptides.
Neurochem Int. 7 (2): 309-17.
References for MBP antibody
-
Groome, N.P. et al. (1986) Region-specific immunoassays for human myelin basic protein.
J Neuroimmunol. 12 (4): 253-64. -
Marignier, R. et al. (2010) Oligodendrocytes are damaged by neuromyelitis optica immunoglobulin G via astrocyte injury.
Brain. 133 (9): 2578-91. -
Brunner, C. et al. (1989) Differential ultrastructural localization of myelin basic protein, myelin/oligodendroglial glycoprotein, and 2',3'-cyclic nucleotide 3'-phosphodiesterase in the CNS of adult rats.
J Neurochem. 52: 296-304. -
Jatana, M. et al. (2006) Combination of systemic hypothermia and N-acetylcysteine attenuates hypoxic-ischemic brain injury in neonatal rats.
Pediatr Res. 59: 684-9. -
Chujor, C.S. et al. (1991) Serum antibodies against peripheral nervous system antigens in leprosy.
Int J Lepr Other Mycobact Dis. 59: 590-7.
Further Reading
-
Martenson, R. E. (1984) Experimental allergic encephalomyelitis. A useful model for multiple sclerosis.
In Alvord, E. C. et al. (Eds).Wiley, New York, pp273-289.
- Synonyms
- Myelin Basic Protein
- RRID
- AB_2140358
- UniProt
- P02687
- Entrez Gene
- MBP
- GO Terms
- GO:0005886 plasma membrane
- GO:0005515 protein binding
- GO:0019911 structural constituent of myelin sheath
- GO:0043209 myelin sheath
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