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MBP antibody | 1

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Mouse anti Bovine MBP (aa129-138)

Product Type
Monoclonal Antibody
Clone
1
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA70
Datasheet
C E
MSDS
2 ml loader

Mouse anti Bovine MBP (aa129-138) antibody, clone 1 recognizes myelin basic protein (MBP). Clone 1 is reactive with an epitope in the 129-138 region of the human MBP molecule. The numbering of MBP residues is that as described by Martenson Martenson, R. E., 1984.

Clone 1 (129-138) has been reported as being suitable for Western blotting.

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Tissue Culture Supernatant - liquid
Preparation
Tissue Culture Supernatant containing 0.1M Tris/HCl pH7.4 and 5-10% foetal calf serum
Preservative Stabilisers
0.05%Sodium Azide
Immunogen
Bovine Myelin Basic Protein (MBP).
Fusion Partners
Spleen cells from immunised NIH mice were fused with cells of the NS0 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

UniProt
P02687 Related reagents
Entrez Gene
MBP Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0019911 structural constituent of myelin sheath
GO:0043209 myelin sheath
Regulatory
For research purposes only

Applications of MBP antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/200 1/1000
Immunohistology - Frozen 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F F 0.1 mg loader
Human anti Mouse IgG2a:HRP HCA037P E 0.1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Product Specific References

References for MBP antibody

  1. Groome, N. et al. (1985) Preparation and properties of monoclonal antibodies to myelin basic protein and its peptides.
    Neurochem Int. 7 (2): 309-17.
  2. Martenson, R. E. (1984) Experimental allergic encephalomyelitis. A useful model for multiple sclerosis.
    In Alvord, E. C. et al. (Eds).Wiley, New York, pp273-289.
  3. Groome, N.P. et al. (1986) Region-specific immunoassays for human myelin basic protein.
    J Neuroimmunol. 12 (4): 253-64.
  4. Marignier, R. et al. (2010) Oligodendrocytes are damaged by neuromyelitis optica immunoglobulin G via astrocyte injury.
    Brain. 133 (9): 2578-91.
  5. Brunner, C. et al. (1989) Differential ultrastructural localization of myelin basic protein, myelin/oligodendroglial glycoprotein, and 2',3'-cyclic nucleotide 3'-phosphodiesterase in the CNS of adult rats.
    J Neurochem. 52: 296-304.
  6. Jatana, M. et al. (2006) Combination of systemic hypothermia and N-acetylcysteine attenuates hypoxic-ischemic brain injury in neonatal rats.
    Pediatr Res. 59: 684-9.
  7. Chujor, C.S. et al. (1991) Serum antibodies against peripheral nervous system antigens in leprosy.
    Int J Lepr Other Mycobact Dis. 59: 590-7.
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