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IL-4 antibody | CC303

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Anti Bovine Interleukin-4 Antibody, clone CC303 thumbnail image 1 Anti Bovine Interleukin-4 Antibody, clone CC303 thumbnail image 2 Anti Bovine Interleukin-4 Antibody, clone CC303 thumbnail image 3
Anti Bovine Interleukin-4 Antibody, clone CC303 gallery image 1
Published customer image:
Mouse anti Bovine interleukin-4 antibody, clone CC303 (MCA1820) used for the identification of interferonγ expressing dog lymphocytes by flow cytometry.
Image caption:
Representative dot plots illustrating the analysis of intracellular cytokine profile in T-cell subsets. (A) Pseudocolor plot distribution of short-term in vitro cultured (control or SLA-Ag stimulated) canine whole blood sample according to cell size (Forward scatter - FSC) and granularity (Side scatter- SSC) used for lymphocyte gate selection (R1) of FSCLowSSCLow events. (B) Pseudocolor plots representing cytokines + (IL-17, TNF-α, IFN-γ, TGF-β and IL-4) CD4+ cells within gated lymphocytes and (C) Pseudocolor plots representing cytokines + (IL-17, TNF-α, IFN-γ, TGF-β and IL-4) CD8+ cells within gated lymphocytes. The frequency of cytokines+ T-cells subsets were calculated by quadrant statistics approach and first reported as percentage of gated lymphocytes prior to the calculation of the SLAg/Control indexes.

From: Costa-Pereira C, Moreira ML, Soares RP, Marteleto BH, Ribeiro VM, França-Dias MH, Cardoso LM, Viana KF, Giunchetti RC, Martins-Filho OA, Araújo MS. One-year timeline kinetics of cytokine-mediated cellular immunity in dogs vaccinated against visceral leishmaniasis. BMC Vet Res. 2015 Apr 11;11(1):92.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Bovine Interleukin-4 Antibody, clone CC303 gallery image 2
Published customer image:
Mouse anti Bovine interleukin-4 antibody, clone CC303 (MCA1820) used for the detection of IL-4 expressing cells in yellow cattle and water buffalo following schistosome infection by flow cytometry.
Image caption:
Percentage of IFN-γ and IL-4 positive cells detected by flow cytometry pre and post infection. yellow cattle; (B) water buffalo. Level of significance when compared to water buffalo group at the same time, *p < 0.05.

Yang J, Fu Z, Feng X, Shi Y, Yuan C, Liu J, Hong Y, Li H, Lu K, Lin J. Comparison of worm development and host immune responses in natural hosts of Schistosoma japonicum, yellow cattle and water buffalo. BMC Vet Res. 2012 Mar 13;8:25.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Bovine Interleukin-4 Antibody, clone CC303 gallery image 3
Published customer image:
Mouse anti Bovine interleukin-4 antibody, clone CC303 (MCA1820) used to evaluate IL-4 expression by canine pbmc using flow cytometry.
Image caption:
Profile of intracytoplasmatic cytokines in peripheral blood mononuclear cells (PBMCs) of dogs naturally infected by Leishmania infantum after immunotherapy with LBMPL vaccine or with MPL adjuvant alone. Pseudocolor plots illustrating the analysis of intracellular cytokines+ (IL-4 and IFN-γ) (A) in CD4+ cells (B) and CD8+ cells (C) within gated lymphocytes in PBMCs after in vitro stimulation with soluble Leishmania infantum antigen (SLiAg) in dogs submitted to immunotherapy with LBMPL vaccine (black rectangle) or MPL adjuvant alone (white rectangle); before (T0) and after 90 days (T90) of treatment. The frequency of cytokines+ T-cell subsets were calculated by quadrant statistics approach and first reported as percentage of gated lymphocytes prior to the calculation of the SLiAg/control indexes. The CD4+ and CD8+ cytokine indexes (B,C) were calculated as the proportion of cytokine+ cells observed in SLiAg-stimulated cultures divided by the control culture (SLAg/CC ratio). Significant differences (P <0.05) are shown by connecting lines—representing differences between the LBMPL and MPL groups—and by the “*”—representing differences between T0 and T90.

From: Roatt BM, Aguiar-Soares RD, Reis LE, Cardoso JM, Mathias FA, de Brito RC, daSilva SM, Gontijo NF, Ferreira SA, Valenzuela JG, Corrêa-Oliveira R, Giunchetti RC, Reis AB.
A Vaccine Therapy for Canine Visceral Leishmaniasis Promoted Significant Improvement of Clinical and Immune Status with Reduction in Parasite Burden.
Front Immunol. 2017 Mar 7;8:217.
doi: 10.3389/fimmu.2017.00217.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Bovine Interleukin-4:FITC

Mouse anti Bovine Interleukin-4

Mouse anti Bovine Interleukin-4:RPE

Product Type
Monoclonal Antibody
Clone
CC303
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA1820F
Datasheet
F*
MSDS
0.1 mg loader

MCA1820
Datasheet
E F*
MSDS
0.5 mg loader

MCA1820PE
Datasheet
F*
MSDS
100 Tests loader

Mouse anti Bovine Interleukin-4 antibody, clone CC303 recognizes bovine interleukin 4

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Dog
Pig
Sheep
Mustelid
Goat
Dolphin
Mink
Fin Whale
Horse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC.

DO NOT FREEZE

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.
Guarantee
18 months from date of despatch.
Guarantee
12 months from date of reconstitution.

More Information

UniProt
P30367 Related reagents
Entrez Gene
IL4 Related reagents
GO Terms
GO:0005125 cytokine activity
GO:0005136 interleukin-4 receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
GO:0030890 positive regulation of B cell proliferation
GO:0042113 B cell activation
GO:0043066 negative regulation of apoptosis
GO:0045348 positive regulation of MHC class II biosynthetic process
GO:0045671 negative regulation of osteoclast differentiation
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0048295 positive regulation of isotype switching to IgE isotypes
GO:0048304 positive regulation of isotype switching to IgG isotypes
Regulatory
For research purposes only

Applications of IL-4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1
ELISA
Flow Cytometry 1 1/50 1/100
Western Blotting
Flow Cytometry 1 Neat 1/5
  1. 1Membrane permeabilization is required for this application. Bio-Rad recommend the use of Leucoperm™ (Product Code BUF09) for this purpose.
  1. 1Membrane permeabilization is required for this application. Bio-Rad recommend the use of Leucoperm™ (Product Code BUF09) for this purpose.
  1. 1Membrane permeabilization is required for this application. Bio-Rad recommend the use of Leucoperm™ (Product Code BUF09) for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/postive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/postive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F F 0.1 mg loader
Human anti Mouse IgG2a:HRP HCA037P E 0.1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG2a Negative Control:FITC MCA929F F 100 Tests loader
Mouse IgG2a Negative Control MCA929 F 100 Tests loader
Mouse IgG2a Negative Control:RPE MCA929PE F 100 Tests loader

Application Based External Images

Flow Cytometry

Product Specific References

References for IL-4 antibody

  1. Pedersen, L.G. et al. (2002) Identification of monoclonal antibodies that cross-react with cytokines from different animal species.
    Vet Immunol Immunopathol. 88 (3-4): 111-22.
  2. Aasted, B. et al. (2002) Cytokine profiles in peripheral blood mononuclear cells and lymph node cells from piglets infected in utero with porcine reproductive and respiratory syndrome virus.
    Clin Diagn Lab Immunol. 9 (6): 1229-34.
  3. Nielsen, L. et al. (2009) Lymphotropism and host responses during acute wild-type canine distemper virus infections in a highly susceptible natural host.
    J Gen Virol. 90: 2157-65.
  4. Jaber, J.R. et al. (2010) Cross-reactivity of anti-human, anti-porcine and anti-bovine cytokine antibodies with cetacean tissues.
    J Comp Pathol. 143: 45-51.
  5. Martel, C.J. & Aasted, B. (2009) Characterization of antibodies against ferret immunoglobulins, cytokines and CD markers.
    Vet Immunol Immunopathol. 132:109-15.
  6. Fellman, C.L. et al. (2011) Cyclosporine A affects the in vitro expression of T cell activation-related molecules and cytokines in dogs.
    Vet Immunol Immunopathol. 140: 175-80.
  7. Araújo, M.S. et al. (2011) Immunological changes in canine peripheral blood leukocytes triggered by immunization with first or second generation vaccines against canine visceral leishmaniasis.
    Vet Immunol Immunopathol. 141: 64-75.
  8. Jensen, P.V. et al. (2003) Cytokine profiles in adult mink infected with Aleutian mink disease parvovirus.
    J Virol. 77: 7444-51.
  9. Papadogiannakis, E.I. et al. (2009) Determination of intracellular cytokines IFN-gamma and IL-4 in canine T lymphocytes by flow cytometry following whole-blood culture.
    Can J Vet Res. 73: 137-43.
  10. Rutigliano, J.A. et al. (2008) Screening monoclonal antibodies for cross-reactivity in the ferret model of influenza infection.
    J Immunol Methods. 336: 71-7.
  11. Wagner, B. et al. (2008) Characterization of monoclonal antibodies to equine interleukin-10 and detection of T regulatory 1 cells in horses.
    Vet Immunol Immunopathol. 122: 57-64.
  12. Hamza, E. et al. (2007) Modulation of allergy incidence in icelandic horses is associated with a change in IL-4-producing T cells.
    Int Arch Allergy Immunol. 144: 325-37.
  13. Costa-Pereira, C. et al. (2015) One-year timeline kinetics of cytokine-mediated cellular immunity in dogs vaccinated against visceral leishmaniasis.
    BMC Vet Res. 11 (1): 92.
  14. Dean, G.S. et al. (2005) Minimum infective dose of Mycobacterium bovis in cattle.
    Infect Immun. 73 (10): 6467-71.
  15. Araújo, M.S. et al. (2009) T-cell-derived cytokines, nitric oxide production by peripheral blood monocytes and seric anti-Leishmania (Leishmania) chagasi IgG subclass patterns following immunization against canine visceral leishmaniasis using Leishvaccine and Leishmune.
    Vaccine. 27 (7): 1008-17.
  16. Yang, J. et al. (2012) Comparison of worm development and host immune responses in natural hosts of Schistosoma japonicum, yellow cattle and water buffalo.
    BMC Vet Res. 8: 25.
  17. Taubert A et al. (2008) Antigen-induced cytokine production in lymphocytes of Eimeria bovis primary and challenge infected calves.
    Vet Immunol Immunopathol. 126 (3-4): 309-20.
  18. Geherin, S.A. et al. (2013) Ovine skin-recirculating γδ T cells express IFN-γ and IL-17 and exit tissue independently of CCR7.
    Vet Immunol Immunopathol. 155 (1-2): 87-97.
  19. Moreira, M.L. et al. (2016) Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes.
    Vet Parasitol. 220: 33-45.
  20. Roatt, B.M. et al. (2017) A Vaccine Therapy for Canine Visceral Leishmaniasis Promoted Significant Improvement of Clinical and Immune Status with Reduction in Parasite Burden.
    Front Immunol. 8: 217.
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