IL-12 / IL-23 antibody | CC301
Image caption:
IL-12 response (u/ml) in mesenteric lymph nodes against GRA7, TLA, MIC3 and EC2 in function of time.
From: Verhelst D, De Craeye S, Entrican G, Dorny P, Cox E.
Parasite distribution and associated immune response during the acute phase of Toxoplasma gondii infection in sheep.
BMC Vet Res. 2014 Dec 16;10(1):293.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Image caption:
IL-12 responses of neonatal and adult MLN cells following TLR agonist stimulation. Neonatal (closed circles) and adult (open squares) MLN cells were cultured in vitro with or without 12.5 μg/ml polyI:C, 0.5 μg/ml R-848 or 5 μg/ml Gardiquimod. Supernatants were harvested after 48 h of culture and ELISA was carried out for IL-12 (A) secretion. Each circle or square represents one neonate or one adult, respectively. Medians are shown for each stimulus. Non-parametric Mann-Whitney tests were used to compare data for neonates and adults: *p≤0.01; **p≤0.005; ***p≤0.001; ****p≤0.0005.
From: Ferret-Bernard S, Remot A, Lacroix-Lamandé S, Metton C, Bernardet N, et al. (2010)
Cellular and Molecular Mechanisms Underlying the Strong Neonatal IL-12 Response of Lamb Mesenteric Lymph Node Cells to R-848.
PLoS ONE 5(10): e13705.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Image caption:
MLN and spleen cell IL-12 responses to R-848 stimulation as a function of age. MLN and spleen cells from 6- to 14-day-old neonates (closed circles), 20-day-old lambs (closed diamonds) and adults (open squares) were cultured in vitro for 48 h, with or without 0.5 μg/ml R-848. At the end of the culture period, supernatants were harvested and ELISA carried out to assess IL-12 secretion by MLN cells (A) or spleen cells (B). Medians are shown for each stimulus. Non-parametric Mann-Whitney tests were used to compare data for neonates, 20-day-old lambs and adults: *p≤0.01; **p≤0.005; ***p≤0.001; ****p≤0.0005.
From: Ferret-Bernard S, Remot A, Lacroix-Lamandé S, Metton C, Bernardet N, et al. (2010)
Cellular and Molecular Mechanisms Underlying the Strong Neonatal IL-12 Response of Lamb Mesenteric Lymph Node Cells to R-848.
PLoS ONE 5(10): e13705.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Image caption:
Roles of TGFβ1 and IL-10 in regulating IL-12 responses to R-848. MLN cells from neonates (closed circles) and adults (open squares) were cultured in vitro for 48 h, with or without 0.5 μg/ml R-848, in the presence of rhTGFβ1 (n = 7) (A) or rovIL-10 (n = 5) (B). Supernatants were harvested and ELISA was carried out to assess IL-12 secretion. The mean ± SEM level of IL-12 secretion is shown (A, B). RNA was extracted and purified from freshly isolated MLN cells. IL-10 mRNA levels were determined by quantitative RT-PCR. Median normalised values are presented for neonates (closed circles) and adults (open squares) (C). MLN cells from neonates (closed circles) and adults (open squares) were stimulated in vitro for 48 h with or without 0.5 μg/ml R-848. Supernatants were harvested and ELISA carried out to assess IL-10 secretion. Medians are indicated. Non-parametric Mann-Whitney tests were used to compare data for neonates and adults: **p≤≤0.001 (D). RNA was extracted and purified from freshly isolated MLN cells. Foxp3 mRNA levels were determined by quantitative RT-PCR. Median normalised values are presented for neonates (closed circles) and adults (open squares) (E). Comparison of the IL-12 response of neonate MLN cells to R-848 stimulation in culture medium supplemented with 10%FCS, 10% neonate autologous plasma or 10% adult plasma. Paired t-test between neonate and adult plasma were non-significant (F).
From: Ferret-Bernard S, Remot A, Lacroix-Lamandé S, Metton C, Bernardet N, et al. (2010)
Cellular and Molecular Mechanisms Underlying the Strong Neonatal IL-12 Response of Lamb Mesenteric Lymph Node Cells to R-848.
PLoS ONE 5(10): e13705.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Image caption:
Main cell population producing IL-12 in response to R-848. Neonatal MLN cells were cultured in vitro with or without 0.5 μg/ml R-848 and, at various time points, supernatants were harvested and ELISA was carried out for IL-12. Mean ± SEM IL-12 secretion is shown for each time point (A). Neonatal MLN cells were cultured in vitro with (black bars) or without (hatched bars) 0.5 μg/ml R-848 for 8 h, with brefeldin A added for the last 5 h. Cells were harvested for IL-12 intracellular staining combined with labelling of CD11b, CD14, MHC class II and CD205. Three independent experiments were carried out and the data shown are the mean proportions of IL-12+ cells after gating on CD11b+, CD14+, MHC class II+ or CD205+ MLN cells (B).
From: Ferret-Bernard S, Remot A, Lacroix-Lamandé S, Metton C, Bernardet N, et al. (2010)
Cellular and Molecular Mechanisms Underlying the Strong Neonatal IL-12 Response of Lamb Mesenteric Lymph Node Cells to R-848.
PLoS ONE 5(10): e13705.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Bovine Interleukin-12/23 antibody, clone CC301 (MCA1782EL) as a capture reagent in a sandwich ELIDA with biotinylated Mouse anti Bovine IL-12/23 antibody, clone CC326 (MCA2173B) as a detection reagent for the measurement of IL-12 secretion by bovine PBMC.
Image caption:
Interleukin-12 secretion by PBMCs unstimulated (UNC) and stimulated with LPS and ConA (SC, 1 and 5 μg/ml, respectively) and with 4 mM of H2O2 for 24 h at 37°C/5% CO2. *P <0.05 were considered significant among feeding strategy.
From: Ciliberti MG, Albenzio M, De Palo P, Santillo A, Caroprese M.
Nexus Between Immune Responses and Oxidative Stress: The Role of Dietary Hydrolyzed Lignin in ex vivo Bovine Peripheral Blood Mononuclear Cell Response.
Front Vet Sci. 2020 Feb 20;7:9.
doi: 10.3389/fvets.2020.00009.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Bovine Interleukin-12/23:Low Endotoxin
- Product Type
- Monoclonal Antibody
- Clone
- CC301
- Isotype
- IgG2a
- Specificity
- IL-12 / IL-23
Antibody Quality and Performance Guarantee
| Mouse anti Bovine Interleukin-12/23 antibody, clone CC301 recognizes the p40 subunit of bovine interleukin-12, and binds to both the free subunit and the intact heterodimer. The p40 subunit is also known as IL-12B and can form a heterodimer with either IL-12A or IL-23A. Mouse anti Bovine Interleukin-12/23 antibody, clone CC301 may be used as a capture antibody in an ELISA in combination with biotinylated Mouse anti Bovine Interleukin-12/23 antibody, clone CC326 (MCA2173B) as a detection reagent. |
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human Sheep Mouse African Buffalo - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant bovine IL-12.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Endotoxin Level
- <0.01 EU/μg
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at -20°C only.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
8ug/ml | |
| ELISpot | |
||
| Flow Cytometry 1 | |
1/10 | |
| Functional Assays | |
1/100 | |
| Western Blotting | |
1/50 | 1/100 |
- 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
- ELISA
- This antibody may be used as a capture antibody in a sandwich ELISA in combination with biotinylated clone CC326 (MCA2173B) as detection reagent (Bannerman et al. 2004).
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2a Negative Control:Low Endotoxin | MCA929EL | F | 0.5 mg |
|
Log in | ||
| List Price | Your Price | ||||||
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| Description | Mouse IgG2a Negative Control:Low Endotoxin | ||||||
References for IL-12 / IL-23 antibody
-
Hope, J.C. et al. (2002) Development of detection methods for ruminant interleukin (IL)-12.
J Immunol Methods. 266 (1-2): 117-26. -
Wenz, J.R. et al. (2010) Factors associated with concentrations of select cytokine and acute phase proteins in dairy cows with naturally occurring clinical mastitis.
J Dairy Sci. 93: 2458-70. -
Rinaldi, M. et al (2010) A sentinel function for teat tissues in dairy cows: dominant innate immune response elements define early response to E. coli mastitis.
Funct Integr Genomics. 10: 21-38. -
Ferret-Bernard, S. et al. (2011) Mesenteric lymph node cells from neonates present a prominent IL-12 response to CpG oligodeoxynucleotide via an IL-15 feedback loop of amplification.
Vet Res. 42:19. -
Contreras, V. et al. (2010) Existence of CD8α-like dendritic cells with a conserved functional specialization and a common molecular signature in distant mammalian species.
J Immunol. 185: 3313-25. -
Bannerman, D.D. et al. (2004) Escherichia coli and Staphylococcus aureus elicit differential innate immune responses following intramammary infection.
Clin Diagn Lab Immunol. 11: 463-72. -
Davis, T.L. and Pate, J.L. (2007) Bovine luteal cells stimulate proliferation of major histocompatibility nonrestricted gamma delta T cells.
Biol Reprod. 77: 914-22. -
Ferret-Bernard, S. (2010) Cellular and molecular mechanisms underlying the strong neonatal IL-12 response of lamb mesenteric lymph node cells to R-848.
PLoS One. 5: e13705.
View The Latest Product References
-
Shoda, L.K. et al. (2001) DNA from protozoan parasites Babesia bovis, Trypanosoma cruzi, and T. brucei is mitogenic for B lymphocytes and stimulates macrophage expression of interleukin-12, tumor necrosis factor alpha, and nitric oxide.
Infect Immun. 69: 2162-71. -
Souza, M. et al. (2008) Pathogenesis and immune responses in gnotobiotic calves after infection with the genogroup II.4-HS66 strain of human norovirus.
J Virol. 82: 1777-86. -
Verhelst, D. et al. (2014) Parasite distribution and associated immune response during the acute phase of Toxoplasma gondii infection in sheep.
BMC Vet Res. 10: 293. -
Beechler BR et al. (2015) Enemies and turncoats: bovine tuberculosis exposes pathogenic potential of Rift Valley fever virus in a common host, African buffalo (Syncerus caffer).
Proc Biol Sci. 282 (1805): . -
Pomeroy B et al. (2015) Monocyte-derived dendritic cells from late gestation cows have an impaired ability to mature in response to E. coli stimulation in a receptor and cytokine-mediated fashion.
Vet Immunol Immunopathol. 167 (1-2): 22-9. -
Stabel, J.R. & Bannantine, J.P. (2021) Reduced tissue colonization of Mycobacterium avium subsp. paratuberculosis in neonatal calves vaccinated with a cocktail of recombinant proteins.
Vaccine. 39 (23): 3131-40. -
Rodrigues, V. et al. (2017) Development of a bead-based multiplexed assay for simultaneous quantification of five bovine cytokines by flow cytometry.
Cytometry A. 91 (9): 901-7. -
Stabel, J.R. et al. (2021) Comparative cellular immune responses in calves after infection with Mycobacterium avium subsp. paratuberculosis, M. avium subsp. avium, M. kansasii and M. bovis.
Vet Immunol Immunopathol. 237: 110268. -
Ciliberti, M.G. et al. (2020) Nexus Between Immune Responses and Oxidative Stress: The Role of Dietary Hydrolyzed Lignin in ex vivo Bovine Peripheral Blood Mononuclear Cell Response.
Front Vet Sci. 7: 9. -
Tavalire, H.F. et al. (2019) Risk alleles for tuberculosis infection associate with reduced immune reactivity in a wild mammalian host.
Proc Biol Sci. 286 (1907): 20190914. -
Stabel, J.R. et al. (2020) Comparison of Sheep, Goats, and Calves as Infection Models for Mycobacterium avium subsp. paratuberculosis.
Vet Immunol Immunopathol. 225: 110060. -
Ihedioha, O. et al. (2020) Poor stimulation of bovine dendritic cells by Mycobacterium bovis culture supernatant and surface extract is associated with decreased activation of ERK and NF-κB and higher expression of SOCS1 and 3.
Innate Immun. 26 (6): 537-546.
- RRID
- AB_616909
- UniProt
- P54349
- P46282
- Entrez Gene
- IL12B
- IL12A
- GO Terms
- GO:0016020 membrane
- GO:0004896 cytokine receptor activity
- GO:0005125 cytokine activity
- GO:0005143 interleukin-12 receptor binding
- GO:0030101 natural killer cell activation
- GO:0042093 T-helper cell differentiation
- GO:0042095 interferon-gamma biosynthetic process
- GO:0042104 positive regulation of activated T cell proliferation
- GO:0008083 growth factor activity
- View More GO Terms
MCA1782EL
1612
163392
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