Mouse anti Bovine IgG2, clone K192 4F10
, is a monoclonal antibody specific for bovine IgG2, recognizing both the homozygous A1/A1 & heterozygous A1/A2 allotypes (Kacskovics & JButler 1996
) and does not recognize other bovine immunoglobulin classes.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Purified Bovine IgG
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of the P3.X63.Ag8.653 myeloma cell line
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of IgG2 antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- This product is suitable for use in direct ELISA applications
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Secondary Antibodies Available
Application Based External Images
Product Specific References
References for IgG2 antibody
Estes, D. M. et al. (1998) Effects of type I/type II interferons and transforming growth factor-beta on B-cell differentiation and proliferation. Definition of costimulation and cytokine requirements for immunoglobulin synthesis and expression.
Immunology 95: 604- 611.
Wyatt, C.R. et al. (2001) Evidence for the emergence of a type-1-like immune response in intestinal mucosa of calves recovering from cryptosporidiosis.
J Parasitol. 87: 90-5.
Abbott, J.R. et al. (2005) Rapid and long-term disappearance of CD4+ T lymphocyte responses specific for Anaplasma marginale major surface protein-2 (MSP2) in MSP2 vaccinates following challenge with live A. marginale.
J Immunol. 174: 6702-15.
Arulkanthan, A. et al. (1999) Biased immunoglobulin G1 isotype responses induced in cattle with DNA expressing msp1a of Anaplasma marginale.
Infect Immun. 67: 3481-7.
Barrio, M.B. et al. (2003) Assessment of the opsonic activity of purified bovine sIgA following intramammary immunization of cows with Staphylococcus aureus.
J Dairy Sci. 86: 2884-94.
French, D.M. et al. (1999) Emergence of Anaplasma marginale antigenic variants during persistent rickettsemia.
Infect Immun. 67: 5834-40.
Han, S. et al. (2010) Anaplasma marginale infection with persistent high-load bacteremia induces a dysfunctional memory CD4+ T lymphocyte response but sustained high IgG titers.
Clin Vaccine Immunol. 17: 1881-90.
Kooyman, F.N. et al. (2007) Antibodies elicited by the bovine lungworm, Dictyocaulus viviparus, cross-react with platelet-activating factor.
Infect Immun. 75: 4456-62.
Morton, H.C. et al. (2001) Identification of residues within the extracellular domain 1 of bovine Fc gamma 2R essential for binding bovine IgG2.
J Biol Chem. 276: 47794-800.
Salem, E. et al. (2019) Pathogenesis, Host Innate Immune Response, and Aerosol Transmission of Influenza D Virus in Cattle.
J Virol. 93(7):e01853-18.
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