IgG2 antibody | K192 4F10
|Mouse anti Bovine IgG2, clone K192 4F10, is a monoclonal antibody specific for bovine IgG2, recognizing both the homozygous A1/A1 & heterozygous A1/A2 allotypes (Kacskovics & JButler 1996) and does not recognize other bovine immunoglobulin classes.|
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Purified Bovine IgG
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of the P3.X63.Ag8.653 myeloma cell line
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
- This product is suitable for use in direct ELISA applications
References for IgG2 antibody
Estes, D. M. et al. (1998) Effects of type I/type II interferons and transforming growth factor-beta on B-cell differentiation and proliferation. Definition of costimulation and cytokine requirements for immunoglobulin synthesis and expression.
Immunology 95: 604- 611.
Wyatt, C.R. et al. (2001) Evidence for the emergence of a type-1-like immune response in intestinal mucosa of calves recovering from cryptosporidiosis.
J Parasitol. 87: 90-5.
Abbott, J.R. et al. (2005) Rapid and long-term disappearance of CD4+ T lymphocyte responses specific for Anaplasma marginale major surface protein-2 (MSP2) in MSP2 vaccinates following challenge with live A. marginale.
J Immunol. 174: 6702-15.
Arulkanthan, A. et al. (1999) Biased immunoglobulin G1 isotype responses induced in cattle with DNA expressing msp1a of Anaplasma marginale.
Infect Immun. 67: 3481-7.
Barrio, M.B. et al. (2003) Assessment of the opsonic activity of purified bovine sIgA following intramammary immunization of cows with Staphylococcus aureus.
J Dairy Sci. 86: 2884-94.
French, D.M. et al. (1999) Emergence of Anaplasma marginale antigenic variants during persistent rickettsemia.
Infect Immun. 67: 5834-40.
Han, S. et al. (2010) Anaplasma marginale infection with persistent high-load bacteremia induces a dysfunctional memory CD4+ T lymphocyte response but sustained high IgG titers.
Clin Vaccine Immunol. 17: 1881-90.
Kooyman, F.N. et al. (2007) Antibodies elicited by the bovine lungworm, Dictyocaulus viviparus, cross-react with platelet-activating factor.
Infect Immun. 75: 4456-62.
View The Latest Product References
Morton, H.C. et al. (2001) Identification of residues within the extracellular domain 1 of bovine Fc gamma 2R essential for binding bovine IgG2.
J Biol Chem. 276: 47794-800.
Salem, E. et al. (2019) Pathogenesis, Host Innate Immune Response, and Aerosol Transmission of Influenza D Virus in Cattle.
J Virol. 93(7):e01853-18.
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