CD8 Beta antibody | CC58

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Anti Bovine CD8 Beta Antibody, clone CC58 thumbnail image 1

Anti Bovine CD8 Beta Antibody, clone CC58 thumbnail image 2

Anti Bovine CD8 Beta Antibody, clone CC58 thumbnail image 3

Anti Bovine CD8 Beta Antibody, clone CC58 gallery image 1 — Figure A. FITC conjugated Mouse anti Bovine CD21 (MCA1424F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Bovine CD21 (MCA1424F) and RPE conjugated Mouse anti Bovine CD8b (**MCA1654PE**). All experiments performed on red cell lysed bovine blood gated on lymphocytes in the presence of 10% bovine serum. Data acquired on the ZE5™ Cell Analyzer.

Anti Bovine CD8 Beta Antibody, clone CC58 gallery image 2 — Figure A. RPE conjugated Mouse anti Bovine CD8b (**MCA1654PE**) and FITC conjugated mouse IgG2b isotype control (MCA691F). Figure B. RPE conjugated mouse anti bovine CD8b (**MCA1645PE**) and FITC conjugated mouse anti bovine CD21 (MCA5953F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

Anti Bovine CD8 Beta Antibody, clone CC58 gallery image 3 — Figure A. FITC conjugated Mouse anti Bovine CD21 (MCA5953F) and RPE conjugated mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated mouse anti bovine CD21 (MCA5953F) and RPE conjugated mouse anti bovine CD8b (**MCA1654PE**). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

Mouse anti Bovine CD8 Beta

Mouse anti Bovine CD8 Beta:RPE

Product Type: Monoclonal Antibody
Clone: CC58
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA1654G	F IP			0.25 mg
MCA1654PE	F			100 Tests

Mouse anti Bovine CD8 beta antibody, clone CC58 recognizes an epitope associated with the bovine CD8 beta chain. CD8 is usually expressed as an α/β heterodimer. Mouse anti Bovine CD8 beta antibody, clone CC58 has been successfully used for the immunohistochemical detection of CD8 on formalin fixed, paraffin embedded placental tissue from water buffalo (Cantón et al. 2014).

Product Details

Target Species

Bovine

Species Cross-Reactivity

Target Species	Cross Reactivity
Sheep
Goat
Water Buffalo

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Product Form

Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized

Reconstitution

Reconstitute with 1 ml distilled water

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%

Sodium Azide

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin
5%	Sucrose

Carrier Free

Yes

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Prior to reconstitution store at +4^oC. Following reconstitution store at +4^oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 12 months from date of reconstitution.

More Information

Regulatory: For research purposes only

Applications of CD8 Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry
Immunoprecipitation
Flow Cytometry	Neat	1/10

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse IgG1 Negative Control	MCA928	100 Tests	F
Mouse IgG1 Negative Control:RPE	MCA928PE	100 Tests	F

Application Based External Images

Flow Cytometry

Product Specific References

References for CD8 Beta antibody

Suraud, V. et al. (2008) Acute infection by conjunctival route with Brucella melitensis induces IgG+ cells and IFN-gamma producing cells in peripheral and mucosal lymph nodes in sheep.
Microbes Infect. 10: 1370-8.
Howard, C.J. & Naessens, J. (1993) Summary of workshop findings for cattle (tables 1 and 2).
Vet Immunol Immunopathol. 39 (1-3): 25-47.
Naessens, J. et al. (1997) Nomenclature and characterization of leukocyte differentiation antigens in ruminants.
Immunol Today. 18 (8): 365-8.
Hein, W.R. et al. (1991) Summary of workshop findings for leukocyte antigens of sheep.
Vet Immunol Immunopathol. 27 (1-3): 28-30.
Gerner, W. et al. (2009) Identification of major histocompatibility complex restriction and anchor residues of foot-and-mouth disease virus-derived bovine T-cell epitopes.
J Virol. 83: 4039-50.
Gerner, W. et al. (2010) Sensitive detection of Foxp3 expression in bovine lymphocytes by flow cytometry.
Vet Immunol Immunopathol. 138: 154-8.
MacHugh, N.D. and Sopp, P. (1991) Individual antigens of cattle. Bovine CD8 (BoCD8).
Vet Immunol Immunopathol. 27: 65-9.
Soltys, J. and Quinn, M.T. (1999) Selective recruitment of T-cell subsets to the udder during staphylococcal and streptococcal mastitis: analysis of lymphocyte subsets and adhesion molecule expression.
Infect Immun. 67: 6293-302.
Cantón, G.J. et al. (2014) Characterization of immune cell infiltration in the placentome of water buffaloes (Bubalus bubalis) infected with neospora caninum during pregnancy.
J Comp Pathol. 150: 463-8.
Wattegedera, S.R. et al. (2017) Enhancing the toolbox to study IL-17A in cattle and sheep.
Vet Res. 48 (1): 20.

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