CD8 Beta antibody | CC58
Mouse anti Bovine CD8 beta antibody, clone CC58 (MCA1654GA) used to demonstrate CD8 expressing cells in bovine placenta by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Examples of different immune cell infiltration in placentomes from N. caninum-inoculated animals. (c) Minimal infiltration of cytotoxic T-cells (CD8+) in a necrotic focus in maternal caruncle of a N. caninum challenged cow culled 28 dpi. Counterstained with haematoxylin. Original magnification 200×.
From: Cantón GJ, Katzer F, Benavides-Silván J, Maley SW, Palarea-Albaladejo J, Pang Y, Smith S, Bartley PM, Rocchi M, Innes EA, Chianini F.
Phenotypic characterisation of the cellular immune infiltrate in placentas of cattle following experimental inoculation with Neospora caninum in late gestation.
Vet Res. 2013 Jul 22;44:60.
doi: 10.1186/1297-9716-44-60..
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Bovine CD8 beta antibody, clone CC58 recognizes an epitope associated with the bovine CD8 beta chain. CD8 is usually expressed as an α/β heterodimer. Mouse anti Bovine CD8 beta antibody, clone CC58 has been successfully used for the immunohistochemical detection of CD8 on formalin fixed, paraffin embedded placental tissue from water buffalo (Cantón et al. 2014).
Product Details
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Sheep Goat Water Buffalo - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4°C or at -20°C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch
- Guarantee
- 12 months from date of reconstitution.
More Information
- Regulatory
- For research purposes only
Applications of CD8 Beta antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | |||
| Flow Cytometry | Neat | 1/10 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
|
| Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests |
|
Product Specific References
References for CD8 Beta antibody
-
Suraud, V. et al. (2008) Acute infection by conjunctival route with Brucella melitensis induces IgG+ cells and IFN-gamma producing cells in peripheral and mucosal lymph nodes in sheep.
Microbes Infect. 10: 1370-8. -
Howard, C.J. & Naessens, J. (1993) Summary of workshop findings for cattle (tables 1 and 2).
Vet Immunol Immunopathol. 39 (1-3): 25-47. -
Naessens, J. et al. (1997) Nomenclature and characterization of leukocyte differentiation antigens in ruminants.
Immunol Today. 18 (8): 365-8. -
Hein, W.R. et al. (1991) Summary of workshop findings for leukocyte antigens of sheep.
Vet Immunol Immunopathol. 27 (1-3): 28-30. -
Gerner, W. et al. (2009) Identification of major histocompatibility complex restriction and anchor residues of foot-and-mouth disease virus-derived bovine T-cell epitopes.
J Virol. 83: 4039-50. -
Gerner, W. et al. (2010) Sensitive detection of Foxp3 expression in bovine lymphocytes by flow cytometry.
Vet Immunol Immunopathol. 138: 154-8. -
MacHugh, N.D. and Sopp, P. (1991) Individual antigens of cattle. Bovine CD8 (BoCD8).
Vet Immunol Immunopathol. 27: 65-9. -
Soltys, J. and Quinn, M.T. (1999) Selective recruitment of T-cell subsets to the udder during staphylococcal and streptococcal mastitis: analysis of lymphocyte subsets and adhesion molecule expression.
Infect Immun. 67: 6293-302. -
Cantón, G.J. et al. (2014) Characterization of immune cell infiltration in the placentome of water buffaloes (Bubalus bubalis) infected with neospora caninum during pregnancy.
J Comp Pathol. 150: 463-8. -
Wattegedera, S.R. et al. (2017) Enhancing the toolbox to study IL-17A in cattle and sheep.
Vet Res. 48 (1): 20. -
Hecker, Y.P. et al. (2015) Cell mediated immune responses in the placenta following challenge of vaccinated pregnant heifers with Neospora caninum.
Vet Parasitol. 214 (3-4): 247-54. -
Olivier, M. et al. (2013) Plasticity of migrating CD1b+ and CD1b- lymph dendritic cells in the promotion of Th1, Th2 and Th17 in response to Salmonella and helminth secretions.
PLoS One. 8 (11): e79537.
