CD8 Beta antibody | CC58

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Mouse anti Bovine CD8 Beta:RPE

Product Type
Monoclonal Antibody
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1654PE F 100 Tests
Mouse anti Bovine CD8 beta antibody, clone CC58 recognizes an epitope associated with the bovine CD8 beta chain. CD8 is usually expressed as an α/β heterodimer. Mouse anti Bovine CD8 beta antibody, clone CC58 has been successfully used for the immunohistochemical detection of CD8 on formalin fixed, paraffin embedded placental tissue from water buffalo (Cantón et al. 2014).

Product Details

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
Water Buffalo
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitute with 1 ml distilled water
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin

Storage Information

Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.


This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

More Information

For research purposes only

Applications of CD8 Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control:RPE MCA928PE 100 Tests F

Application Based External Images

Flow Cytometry

Product Specific References

References for CD8 Beta antibody

  1. Suraud, V. et al. (2008) Acute infection by conjunctival route with Brucella melitensis induces IgG+ cells and IFN-gamma producing cells in peripheral and mucosal lymph nodes in sheep.
    Microbes Infect. 10: 1370-8.
  2. Howard, C.J. & Naessens, J. (1993) Summary of workshop findings for cattle (tables 1 and 2).
    Vet Immunol Immunopathol. 39 (1-3): 25-47.
  3. Naessens, J. et al. (1997) Nomenclature and characterization of leukocyte differentiation antigens in ruminants.
    Immunol Today. 18 (8): 365-8.
  4. Hein, W.R. et al. (1991) Summary of workshop findings for leukocyte antigens of sheep.
    Vet Immunol Immunopathol. 27 (1-3): 28-30.
  5. Gerner, W. et al. (2009) Identification of major histocompatibility complex restriction and anchor residues of foot-and-mouth disease virus-derived bovine T-cell epitopes.
    J Virol. 83: 4039-50.
  6. Gerner, W. et al. (2010) Sensitive detection of Foxp3 expression in bovine lymphocytes by flow cytometry.
    Vet Immunol Immunopathol. 138: 154-8.
  7. MacHugh, N.D. and Sopp, P. (1991) Individual antigens of cattle. Bovine CD8 (BoCD8).
    Vet Immunol Immunopathol. 27: 65-9.
  8. Soltys, J. and Quinn, M.T. (1999) Selective recruitment of T-cell subsets to the udder during staphylococcal and streptococcal mastitis: analysis of lymphocyte subsets and adhesion molecule expression.
    Infect Immun. 67: 6293-302.
  9. Cantón, G.J. et al. (2014) Characterization of immune cell infiltration in the placentome of water buffaloes (Bubalus bubalis) infected with neospora caninum during pregnancy.
    J Comp Pathol. 150: 463-8.
  10. Wattegedera, S.R. et al. (2017) Enhancing the toolbox to study IL-17A in cattle and sheep.
    Vet Res. 48 (1): 20.