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CD62L antibody | CC32

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Anti Bovine CD62L Antibody, clone CC32 thumbnail image 1 Anti Bovine CD62L Antibody, clone CC32 thumbnail image 2 Anti Bovine CD62L Antibody, clone CC32 thumbnail image 3 Anti Bovine CD62L Antibody, clone CC32 thumbnail image 4 Anti Bovine CD62L Antibody, clone CC32 thumbnail image 5
Anti Bovine CD62L Antibody, clone CC32 gallery image 1
Figure A. RPE conjugated Mouse anti Bovine CD4 (MCA1653PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated Mouse anti Bovine CD4 (MCA1653PE) and FITC conjugated Mouse anti Bovine CD62L (MCA1649F). All experiments performed on red cell lysed bovine blood gated on lymphocytes in the presence of 10% bovine serum. Data acquired on the ZE5™ Cell Analyzer.
Anti Bovine CD62L Antibody, clone CC32 gallery image 2
Published customer image:
Mouse anti Bovine CD62L antibody, clone CC32 (MCA1649G) used for the evaluation of L-selectin expression on PBMC from M. bovis cattle by flow cytometry.
Image caption:
Characterisation of memory markers reveals a characteristic TEM phenotype for multifunctional CD4+ cells in naturally infected cattle. PBMC from naturally M. bovis infected cattle were stimulated with PPD-B and stained for production of cytokine by ICS flow cytometry. Histograms were first gated on singlet, live lymphocytes and gating strategies applied to allow phenotyping of cells for expression of CD44, CD45RO and CD62L. The quadrant gate defining expression of CD44 and CD62L (top panel), CD45RO and CD62L (middle panel) or CD44 and CD45RO (bottom panel) was determined using the total live-lymphocyte population, as shown for each surface marker combination in the histograms on the far left of the panel. This quadrant gating strategy was used to determine the surface phenotype of total CD4+IFN-γ+, CD4+IL-2+, CD4+TNF-α+ and triple functional CD4+ cells, as shown in the other histograms. The percentage of cytokine producing CD44hiCD62Llo, CD45RO+CD62Llo and CD44hiCD45RO+ is shown for each of these CD4+ populations. The data is from one representative animal of 5 analysed.

From: Whelan AO, Villarreal-Ramos B, Vordermeier HM, Hogarth PJ (2011)
Development of an Antibody to Bovine IL-2 Reveals Multifunctional CD4 TEM Cells in Cattle Naturally Infected with Bovine Tuberculosis.
PLoS ONE 6(12): e29194.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Bovine CD62L Antibody, clone CC32 gallery image 3
Figure A. FITC conjugated mouse anti bovine CD62L (MCA1649F) and RPE conjugated mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated mouse anti bovine CD62L (MCA1649F) and RPE conjugated mouse anti bovine CD80 (MCA2436PE). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Anti Bovine CD62L Antibody, clone CC32 gallery image 4
Figure A. RPE conjugated mouse anti bovine CD80 (MCA2436PE) and FITC conjugated mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated mouse anti bovine CD80 (MCA2436PE) and FITC conjugated mouse anti bovine CD62L (MCA1649F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Anti Bovine CD62L Antibody, clone CC32 gallery image 5
Published customer image:
Phycoerythrin conjugated Mouse anti Bovine CD335 antibody, clone AKS1 (MCA2365PE) used along with FITC conjugated Mouse anti Bovine CD62L antibody, clone CC32 (MCA1649F) to evaluate NKp46 expression on bovine lymphocyte populations by flow cytometry.
Image caption:
CD62L expression by afferent‐lymph (AL) ‐derived CD2− natural killer (NK) cells may permit homing to lymph nodes (LNs). Lymphocytes from peripheral blood (PB), AL and LNs from seven calves were labelled with monoclonal antibodies for NKp46 and CD62L and analysed by flow cytometry. FACS plots from one representative animal illustrate the expression of NKp46 and CD62L by PB‐, AL‐ and LN‐derived lymphocytes (a). Gates were set based on Fluorescence Minus One controls.

From: Hamilton CA, Mahan S, Bell CR, Villarreal-Ramos B, Charleston B, Entrican G, Hope JC.
Frequency and phenotype of natural killer cells and natural killer cell subsets in bovine lymphoid compartments and blood.
Immunology. 2017 May;151(1):89-97.
doi: 10.1111/imm.12708.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Mouse anti Bovine CD62L:Alexa Fluor® 647

Mouse anti Bovine CD62L:FITC

Mouse anti Bovine CD62L

Product Type
Monoclonal Antibody
Clone
CC32
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA1649A647
Datasheet
F
SDS
100 Tests/1ml loader

MCA1649F
Datasheet
F
SDS
0.1 mg loader

MCA1649G
Datasheet
F
SDS
0.25 mg loader

Mouse anti Bovine CD62L antibody, clone CC32 recognizes bovine L-selectin, also known as CD62L, Leukocyte-endothelial cell adhesion molecule 1, LECAM-1 or Lymph node homing receptor. Bovine CD62L is a 370 amino acid ~90 kDa, single pass type I transmembrane glycoprotein bearing a single C-type lectin domain, an EGF-like domain and two Sushi domains (UniProt: P98131).

Immunoprecipitation of peripheral blood mononuclear cell lysates with Mouse anti Bovine CD62L antibody, clone CC32 reveals a molecule of ~90 kDa when run on polyacrylamide gels under reducing conditions, slightly larger than the murine and human CD62L homologues. Bovine CD62L is expressed on subpopulations of T-lymphocytes expressing CD2, CD4 and CD8. WC1 positive γ/δ T cells also express CD62L as do a subpopulation of WC3+ve B-lymphocytes and all peripheral blood monocytes (Howard et al. 1992).

Mouse anti Bovine CD62L antibody, clone CC32 has also been used successfully for the identification of CD62L on ovine peripheral blood cells by flow cytometry (Halliday et al. 2005).

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor 647 - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Bovine lymphocytes
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the NS1 myeloma cell line.

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P98131
Entrez Gene
SELL
GO Terms
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0005529 sugar binding
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD62L antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry Neat 1/10
Flow Cytometry 1/50 1/100
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 647 MCA928A647 F 100 Tests/1ml loader
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
Mouse IgG1 Negative Control MCA928 F 100 Tests loader

Application Based External Images

Flow Cytometry

Immunohistology - Frozen

Product Specific References

References for CD62L antibody

  1. Sopp, P. & Howard, C.J. (2001) IFN gamma and IL-4 production by CD4, CD8 and WC1 gamma delta TCR(+) T cells from cattle lymph nodes and blood.
    Vet Immunol Immunopathol. 81 (1-2): 85-96.
  2. Toka, F.N. et al. (2011) Rapid and Transient Activation of {gamma}{delta} T Cells to IFN-{gamma} Production, NK Cell-Like Killing, and Antigen Processing during Acute Virus Infection.
    J Immunol. 186: 4853-61.
  3. Vesosky, B. et al. (2003) Activation marker expression on bovine peripheral blood gammadelta T cells during post-natal development and following vaccination with a commercial polyvalent viral vaccine.
    Dev Comp Immunol. 27: 439-47.
  4. Edwards, J.C. et al. (2010) PrP(Sc) is associated with B cells in the blood of scrapie-infected sheep.
    Virology. 405: 110-9.
  5. Glew, E.J. et al. (2003) Differential effects of bovine viral diarrhoea virus on monocytes and dendritic cells.
    J Gen Virol. 84: 1771-80.
  6. Vrieling M et al. (2012) γδ T cell homing to skin and migration to skin-draining lymph nodes is CCR7 independent.
    J Immunol. 188 (2): 578-84.
  7. Brackenbury, L.S. et al. (2005) Identification of a cell population that produces alpha/beta interferon in vitro and in vivo in response to noncytopathic bovine viral diarrhea virus.
    J Virol. 79: 7738-44.
  8. Whelan, A.O. et al. (2011) Development of an Antibody to Bovine IL-2 Reveals Multifunctional CD4 T(EM) Cells in Cattle Naturally Infected with Bovine Tuberculosis.
    PLoS One. 6: e29194.
  9. Riollet, C. et al. (2001) Cell subpopulations and cytokine expression in cow milk in response to chronic Staphylococcus aureus infection.
    J Dairy Sci. 84: 1077-84.
  10. Ozawa, T. et al. (2011) Effect of intramammary infusion of rbGM-CSF on SCC and expression of polymorphonuclear neutrophil adhesion molecules in subclinical mastitis cows.
    Vet Res Commun. 36: 21-7.
  11. Silvestre, F.T. et al. (2011) Effects of differential supplementation of fatty acids during the peripartum and breeding periods of Holstein cows: II. Neutrophil fatty acids and function, and acute phase proteins.
    J Dairy Sci. 94: 2285-301.
  12. Halliday, S. et al. (2005) Expression of PrPC on cellular components of sheep blood.
    J Gen Virol. 86: 1571-9.
  13. Blunt, L. et al. (2015) Phenotypic characterization of bovine memory cells responding to mycobacteria in IFNγ enzyme linked immunospot assays.
    Vaccine. 33 (51): 7276-82.
  14. Hussen, J. et al. (2016) Neutrophil degranulation differentially modulates phenotype and function of bovine monocyte subsets.
    Innate Immun. 22 (2): 124-37.
  15. Chen, X. et al. (2016) Bovine P-selectin mediates leukocyte adhesion and is highly polymorphic in dairy breeds.
    Res Vet Sci. 108: 85-92.
  16. Jimbo, S. et al. (2017) Effect of Mycoplasma bovis on bovine neutrophils.
    Vet Immunol Immunopathol. 188: 27-33.
  17. Hamilton, C.A. et al. (2017) Frequency and phenotype of natural killer cells and natural killer cell subsets in bovine lymphoid compartments and blood.
    Immunology. 151 (1): 89-97.
  18. Souza, F.N. et al. (2020) Immune response in nonspecific mastitis: What can it tell us?
    J Dairy Sci. 103 (6): 5376-86.

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