CD62L antibody | CC32

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Anti Bovine CD62L Antibody, clone CC32 thumbnail image 1

Anti Bovine CD62L Antibody, clone CC32 thumbnail image 2

Anti Bovine CD62L Antibody, clone CC32 thumbnail image 3

Anti Bovine CD62L Antibody, clone CC32 thumbnail image 4

Anti Bovine CD62L Antibody, clone CC32 gallery image 1 — Figure A. RPE conjugated Mouse anti Bovine CD4 (MCA1653PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated Mouse anti Bovine CD4 (MCA1653PE) and FITC conjugated Mouse anti Bovine CD62L (**MCA1649F**). All experiments performed on red cell lysed bovine blood gated on lymphocytes in the presence of 10% bovine serum. Data acquired on the ZE5™ Cell Analyzer.

Anti Bovine CD62L Antibody, clone CC32 gallery image 2 — **Published customer image:**
Mouse anti Bovine CD62L antibody, clone CC32 (**MCA1649G**) used for the evaluation of L-selectin expression on PBMC from *M. bovis* cattle by flow cytometry.
**Image caption:**
Characterisation of memory markers reveals a characteristic TEM phenotype for multifunctional CD4+ cells in naturally infected cattle. PBMC from naturally *M. bovis* infected cattle were stimulated with PPD-B and stained for production of cytokine by ICS flow cytometry. Histograms were first gated on singlet, live lymphocytes and gating strategies applied to allow phenotyping of cells for expression of CD44, CD45RO and CD62L. The quadrant gate defining expression of CD44 and CD62L (top panel), CD45RO and CD62L (middle panel) or CD44 and CD45RO (bottom panel) was determined using the total live-lymphocyte population, as shown for each surface marker combination in the histograms on the far left of the panel. This quadrant gating strategy was used to determine the surface phenotype of total CD4+IFN-γ+, CD4+IL-2+, CD4+TNF-α+ and triple functional CD4+ cells, as shown in the other histograms. The percentage of cytokine producing CD44hiCD62Llo, CD45RO+CD62Llo and CD44hiCD45RO+ is shown for each of these CD4+ populations. The data is from one representative animal of 5 analysed.

From: Whelan AO, Villarreal-Ramos B, Vordermeier HM, Hogarth PJ (2011)
Development of an Antibody to Bovine IL-2 Reveals Multifunctional CD4 TEM Cells in Cattle Naturally Infected with Bovine Tuberculosis.
PLoS ONE 6(12): e29194.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Anti Bovine CD62L Antibody, clone CC32 gallery image 3 — Figure A. FITC conjugated mouse anti bovine CD62L (**MCA1649F**) and RPE conjugated mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated mouse anti bovine CD62L (**MCA1649F**) and RPE conjugated mouse anti bovine CD80 (MCA2436PE). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

Anti Bovine CD62L Antibody, clone CC32 gallery image 4 — Figure A. RPE conjugated mouse anti bovine CD80 (MCA2436PE) and FITC conjugated mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated mouse anti bovine CD80 (MCA2436PE) and FITC conjugated mouse anti bovine CD62L (**MCA1649F**). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

Mouse anti Bovine CD62L:FITC

Mouse anti Bovine CD62L

Product Type: Monoclonal Antibody
Clone: CC32
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA1649F	F			0.1 mg
MCA1649G	F			0.25 mg

Mouse anti Bovine CD62L antibody, clone CC32 recognizes bovine L-selectin, also known as CD62L, Leukocyte-endothelial cell adhesion molecule 1, LECAM-1 or Lymph node homing receptor. Bovine CD62L is a 370 amino acid ~90 kDa, single pass type I transmembrane glycoprotein bearing a single C-type lectin domain, an EGF-like domain and two Sushi domains (UniProt: P98131).

Immunoprecipitation of peripheral blood mononuclear cell lysates with Mouse anti Bovine CD62L antibody, clone CC32 reveals a molecule of ~90 kDa when run on polyacrylamide gels under reducing conditions, slightly larger than the murine and human CD62L homologues. Bovine CD62L is expressed on subpopulations of T-lymphocytes expressing CD2, CD4 and CD8. WC1 positive γ/δ T cells also express CD62L as do a subpopulation of WC3^+ve B-lymphocytes and all peripheral blood monocytes (Howard et al. 1992).

Mouse anti Bovine CD62L antibody, clone CC32 has also been used successfully for the identification of CD62L on ovine peripheral blood cells by flow cytometry (Halliday et al. 2005).

Product Details

Target Species

Bovine

Species Cross-Reactivity

Target Species	Cross Reactivity
Sheep

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09%

Sodium Azide

Carrier Free

Yes

Immunogen

Bovine lymphocytes

Approx. Protein Concentrations

IgG concentration 0.1 mg/ml

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Fusion Partners

Spleen cells from immunised BALB/c mice were fused with cells of the NS1 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P98131 Related reagents
Entrez Gene: SELL Related reagents
GO Terms: GO:0007155 cell adhesion; GO:0016021 integral to membrane; GO:0005529 sugar binding
Regulatory: For research purposes only

Applications of CD62L antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry		Neat	1/10
Flow Cytometry		1/50	1/100

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse IgG1 Negative Control:FITC	MCA928F	100 Tests	F
Mouse IgG1 Negative Control	MCA928	100 Tests	F

Application Based External Images

Flow Cytometry

Immunohistology - Frozen

Product Specific References

References for CD62L antibody

Sopp, P. & Howard, C.J. (2001) IFN gamma and IL-4 production by CD4, CD8 and WC1 gamma delta TCR(+) T cells from cattle lymph nodes and blood.
Vet Immunol Immunopathol. 81 (1-2): 85-96.
Toka, F.N. et al. (2011) Rapid and Transient Activation of {gamma}{delta} T Cells to IFN-{gamma} Production, NK Cell-Like Killing, and Antigen Processing during Acute Virus Infection.
J Immunol. 186: 4853-61.
Vesosky, B. et al. (2003) Activation marker expression on bovine peripheral blood gammadelta T cells during post-natal development and following vaccination with a commercial polyvalent viral vaccine.
Dev Comp Immunol. 27: 439-47.
Edwards, J.C. et al. (2010) PrP^(Sc) is associated with B cells in the blood of scrapie-infected sheep.
Virology. 405: 110-9.
Glew, E.J. et al. (2003) Differential effects of bovine viral diarrhoea virus on monocytes and dendritic cells.
J Gen Virol. 84: 1771-80.
Vrieling M et al. (2012) γδ T cell homing to skin and migration to skin-draining lymph nodes is CCR7 independent.
J Immunol. 188 (2): 578-84.
Brackenbury, L.S. et al. (2005) Identification of a cell population that produces alpha/beta interferon in vitro and in vivo in response to noncytopathic bovine viral diarrhea virus.
J Virol. 79: 7738-44.
Whelan, A.O. et al. (2011) Development of an Antibody to Bovine IL-2 Reveals Multifunctional CD4 T(EM) Cells in Cattle Naturally Infected with Bovine Tuberculosis.
PLoS One. 6: e29194.
Riollet, C. et al. (2001) Cell subpopulations and cytokine expression in cow milk in response to chronic Staphylococcus aureus infection.
J Dairy Sci. 84: 1077-84.
Ozawa, T. et al. (2011) Effect of intramammary infusion of rbGM-CSF on SCC and expression of polymorphonuclear neutrophil adhesion molecules in subclinical mastitis cows.
Vet Res Commun. 36: 21-7.
Silvestre, F.T. et al. (2011) Effects of differential supplementation of fatty acids during the peripartum and breeding periods of Holstein cows: II. Neutrophil fatty acids and function, and acute phase proteins.
J Dairy Sci. 94: 2285-301.
Halliday, S. et al. (2005) Expression of PrPC on cellular components of sheep blood.
J Gen Virol. 86: 1571-9.
Blunt, L. et al. (2015) Phenotypic characterization of bovine memory cells responding to mycobacteria in IFNγ enzyme linked immunospot assays
Vaccine. Oct 27 [Epub ahead of print]
Hussen, J. et al. (2016) Neutrophil degranulation differentially modulates phenotype and function of bovine monocyte subsets.
Innate Immun. 22 (2): 124-37.
Chen, X. et al. (2016) Bovine P-selectin mediates leukocyte adhesion and is highly polymorphic in dairy breeds.
Res Vet Sci. 108: 85-92.
Jimbo, S. et al. (2017) Effect of Mycoplasma bovis on bovine neutrophils.
Vet Immunol Immunopathol. 188: 27-33.

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