CD62L antibody | CC32
Mouse anti Bovine CD62L antibody, clone CC32 (MCA1649G) used for the evaluation of L-selectin expression on PBMC from M. bovis cattle by flow cytometry.
Image caption:
Characterisation of memory markers reveals a characteristic TEM phenotype for multifunctional CD4+ cells in naturally infected cattle. PBMC from naturally M. bovis infected cattle were stimulated with PPD-B and stained for production of cytokine by ICS flow cytometry. Histograms were first gated on singlet, live lymphocytes and gating strategies applied to allow phenotyping of cells for expression of CD44, CD45RO and CD62L. The quadrant gate defining expression of CD44 and CD62L (top panel), CD45RO and CD62L (middle panel) or CD44 and CD45RO (bottom panel) was determined using the total live-lymphocyte population, as shown for each surface marker combination in the histograms on the far left of the panel. This quadrant gating strategy was used to determine the surface phenotype of total CD4+IFN-γ+, CD4+IL-2+, CD4+TNF-α+ and triple functional CD4+ cells, as shown in the other histograms. The percentage of cytokine producing CD44hiCD62Llo, CD45RO+CD62Llo and CD44hiCD45RO+ is shown for each of these CD4+ populations. The data is from one representative animal of 5 analysed.
From: Whelan AO, Villarreal-Ramos B, Vordermeier HM, Hogarth PJ (2011)
Development of an Antibody to Bovine IL-2 Reveals Multifunctional CD4 TEM Cells in Cattle Naturally Infected with Bovine Tuberculosis.
PLoS ONE 6(12): e29194.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Bovine CD62L antibody, clone CC32 recognizes bovine L-selectin, also known as CD62L, Leukocyte-endothelial cell adhesion molecule 1, LECAM-1 or Lymph node homing receptor. Bovine CD62L is a 370 amino acid ~90 kDa, single pass type I transmembrane glycoprotein bearing a single C-type lectin domain, an EGF-like domain and two Sushi domains (UniProt: P98131).
Immunoprecipitation of peripheral blood mononuclear cell lysates with Mouse anti Bovine CD62L antibody, clone CC32 reveals a molecule of ~90 kDa when run on polyacrylamide gels under reducing conditions, slightly larger than the murine and human CD62L homologues. Bovine CD62L is expressed on subpopulations of T-lymphocytes expressing CD2, CD4 and CD8. WC1 positive γ/δ T cells also express CD62L as do a subpopulation of WC3+ve B-lymphocytes and all peripheral blood monocytes (Howard et al. 1992).
Mouse anti Bovine CD62L antibody, clone CC32 has also been used successfully for the identification of CD62L on ovine peripheral blood cells by flow cytometry (Halliday et al. 2005).
Immunoprecipitation of peripheral blood mononuclear cell lysates with Mouse anti Bovine CD62L antibody, clone CC32 reveals a molecule of ~90 kDa when run on polyacrylamide gels under reducing conditions, slightly larger than the murine and human CD62L homologues. Bovine CD62L is expressed on subpopulations of T-lymphocytes expressing CD2, CD4 and CD8. WC1 positive γ/δ T cells also express CD62L as do a subpopulation of WC3+ve B-lymphocytes and all peripheral blood monocytes (Howard et al. 1992).
Mouse anti Bovine CD62L antibody, clone CC32 has also been used successfully for the identification of CD62L on ovine peripheral blood cells by flow cytometry (Halliday et al. 2005).
Product Details
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Sheep - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Bovine lymphocytes
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the NS1 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P98131 Related reagents
- Entrez Gene
- SELL Related reagents
- GO Terms
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0005529 sugar binding
- Regulatory
- For research purposes only
Applications of CD62L antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | 1/50 | 1/100 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:FITC | MCA928F | 100 Tests | F | ||
| Mouse IgG1 Negative Control | MCA928 | 100 Tests | F |
Product Specific References
References for CD62L antibody
-
Sopp, P. & Howard, C.J. (2001) IFN gamma and IL-4 production by CD4, CD8 and WC1 gamma delta TCR(+) T cells from cattle lymph nodes and blood.
Vet Immunol Immunopathol. 81 (1-2): 85-96. -
Toka, F.N. et al. (2011) Rapid and Transient Activation of {gamma}{delta} T Cells to IFN-{gamma} Production, NK Cell-Like Killing, and Antigen Processing during Acute Virus Infection.
J Immunol. 186: 4853-61. -
Vesosky, B. et al. (2003) Activation marker expression on bovine peripheral blood gammadelta T cells during post-natal development and following vaccination with a commercial polyvalent viral vaccine.
Dev Comp Immunol. 27: 439-47. -
Edwards, J.C. et al. (2010) PrP(Sc) is associated with B cells in the blood of scrapie-infected sheep.
Virology. 405: 110-9. -
Glew, E.J. et al. (2003) Differential effects of bovine viral diarrhoea virus on monocytes and dendritic cells.
J Gen Virol. 84: 1771-80. -
Vrieling M et al. (2012) γδ T cell homing to skin and migration to skin-draining lymph nodes is CCR7 independent.
J Immunol. 188 (2): 578-84. -
Brackenbury, L.S. et al. (2005) Identification of a cell population that produces alpha/beta interferon in vitro and in vivo in response to noncytopathic bovine viral diarrhea virus.
J Virol. 79: 7738-44. -
Whelan, A.O. et al. (2011) Development of an Antibody to Bovine IL-2 Reveals Multifunctional CD4 T(EM) Cells in Cattle Naturally Infected with Bovine Tuberculosis.
PLoS One. 6: e29194. -
Riollet, C. et al. (2001) Cell subpopulations and cytokine expression in cow milk in response to chronic Staphylococcus aureus infection.
J Dairy Sci. 84: 1077-84. -
Ozawa, T. et al. (2011) Effect of intramammary infusion of rbGM-CSF on SCC and expression of polymorphonuclear neutrophil adhesion molecules in subclinical mastitis cows.
Vet Res Commun. 36: 21-7. -
Silvestre, F.T. et al. (2011) Effects of differential supplementation of fatty acids during the peripartum and breeding periods of Holstein cows: II. Neutrophil fatty acids and function, and acute phase proteins.
J Dairy Sci. 94: 2285-301. -
Halliday, S. et al. (2005) Expression of PrPC on cellular components of sheep blood.
J Gen Virol. 86: 1571-9. -
Blunt, L. et al. (2015) Phenotypic characterization of bovine memory cells responding to mycobacteria in IFNγ enzyme linked immunospot assays
Vaccine. Oct 27 [Epub ahead of print] -
Hussen, J. et al. (2016) Neutrophil degranulation differentially modulates phenotype and function of bovine monocyte subsets.
Innate Immun. 22 (2): 124-37. -
Chen, X. et al. (2016) Bovine P-selectin mediates leukocyte adhesion and is highly polymorphic in dairy breeds.
Res Vet Sci. 108: 85-92. -
Jimbo, S. et al. (2017) Effect of Mycoplasma bovis on bovine neutrophils.
Vet Immunol Immunopathol. 188: 27-33.
