CD45RO antibody | IL-A116
PE conjugated Mouse anti Bovine CD45RO antibody, clone IL-A166 (MCA2434PE) used to evaluate CD45RO expression on caprine PBMC by flow cytometry.
Image caption:
Gating strategy for the determination of frequency of the IFN-γ positive lymphocyte subsets. Peripheral blood mononuclear cells (PBMC) from all goats were cultured in the presence of M. bovis tuberculin (PPD-B). (A,B) Singlet lymphocytes were identified based on the degree of cellular differentiation determined by forward scatter (FSC) and side scatter (SCC). (C) Representative frequencies of the CD4/CD45RO cell populations. (D) Representative frequencies of intracellular IFN-γ staining of cells gated from prelabelled CD4+CD45RO+.
From: Arrieta-Villegas C, Vidal E, Martín M, Verdés J, Moll X, Espada Y, Singh M, Villarreal-Ramos B, Domingo M, Pérez de Val B.
Immunogenicity and Protection against Mycobacterium caprae. Challenge in Goats Vaccinated with BCG and Revaccinated after One Year.
Vaccines (Basel). 2020 Dec 10;8(4):751.
doi: 10.3390/vaccines8040751..
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Bovine CD45RO
- Product Type
- Monoclonal Antibody
- Clone
- IL-A116
- Isotype
- IgG3
- Specificity
- CD45RO
Antibody Quality and Performance Guarantee
| Mouse anti Bovine CD45RO, clone IL-A116 recognizes the bovine homologue of the human CD45RO cell surface antigen. CD45, also known as Leucocyte Common Antigen or LCA, occurs in a number of isoforms, clone IL-A116 is specific for the low molecular weight isoform termed CD45RO, the isoform associated with expression on memory T-cells. Bovine CD45RO is expressed by monocytes, granulocytes and subsets of thymocytes, CD4+ T cells and CD8+ T cells. CD45RO+ CD8+ T cells increase from approximately 5% in neonatal calves to approximately 35% in adult cattle over the age of 5 years (Hogg et al. 2011). Mouse anti Bovine CD45RO, clone IL-A116 immunoprecipitates a molecule of ~180 kDa (Bembridge et al. 1995), analogus to the molecular weight of human and mouse CD45RO. Mouse anti Bovine CD45RO, clone IL-A116 recognizes the CD45RO cell surface antigen by flow cytometry in both European cattle, Bos taurus, and in Zebu, B.indicus (Bembridge et al. 1995). |
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Goat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Bovine peripheral blood monouclear cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/10 | 1/50 |
| Immunoprecipitation | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
References for CD45RO antibody
-
Howard, C.J. & Naessens, J. (1993) Summary of workshop findings for cattle (tables 1 and 2).
Vet Immunol Immunopathol. 39 (1-3): 25-47. -
Naessens, J. et al. (1993) Cross-reactivity of workshop antibodies with cells from domestic and wild ruminants.
Vet Immunol Immunopathol. 39 (1-3): 283-90. -
Bembridge, G.P. et al. (1993) Comparison of monoclonal antibodies with potential specificity for restricted isoforms of the leukocyte common antigen (CD45R).
Vet Immunol Immunopathol. 39 (1-3): 129-36. -
Bembridge, G.P. et al. (1995) CD45RO expression on bovine T cells: relation to biological function.
Immunology. 86 (4): 537-44. -
Mcinnes, E. et al. (1999) Phenotypic analysis of local cellular responses in calves infected with bovine respiratory syncytial virus.
Immunology. 96 (3): 396-403. -
Sopp, P. & Howard, C.J. (2001) IFN gamma and IL-4 production by CD4, CD8 and WC1 gamma delta TCR(+) T cells from cattle lymph nodes and blood.
Vet Immunol Immunopathol. 81 (1-2): 85-96. -
Hogg, A.E. et al. (2011) Characterization of age-related changes in bovine CD8+ T-cells.
Vet Immunol Immunopathol. 140 (1-2): 47-54. -
Whelan, A.O. et al. (2011) Development of an antibody to bovine IL-2 reveals multifunctional CD4 T(EM) cells in cattle naturally infected with bovine tuberculosis.
PLoS One. 6 (12): e29194.
View The Latest Product References
-
Schmidt, N. et al. (2018) Decreased STEC shedding by cattle following passive and active vaccination based on recombinant Escherichia coli Shiga toxoids.
Vet Res. 49 (1): 28. -
Arrieta-Villegas, C. et al. (2020) Immunogenicity and Protection against Mycobacterium caprae Challenge in Goats Vaccinated with BCG and Revaccinated after One Year.
Vaccines (Basel). 8 (4): 751. -
Wherry, T.L.T. et al. (2022) Effects of 1,25-Dihydroxyvitamin D3 and 25-Hydroxyvitamin D3 on PBMCs From Dairy Cattle Naturally Infected With Mycobacterium avium subsp. paratuberculosis.
Front Vet Sci. 9: 830144. -
Hidalgo-Ruiz, M. et al. (2022) Babesia bovis AMA-1, MSA-2c and RAP-1 contain conserved B and T-cell epitopes, which generate neutralizing antibodies and a long-lasting Th1 immune response in vaccinated cattle.
Vaccine. S0264-410X(22)00049-4. -
Seemann, L. et al. (2024) Dietary L-carnitine supplementation modifies blood parameters of mid-lactating dairy cows during standardized lipopolysaccharide-induced inflammation.
Front Immunol. 15: 1390137.
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