CD45RO antibody | IL-A116
PE conjugated Mouse anti Bovine CD45RO antibody, clone IL-A166 (MCA2434PE) used to evaluate CD45RO expression on caprine PBMC by flow cytometry.
Image caption:
Gating strategy for the determination of frequency of the IFN-γ positive lymphocyte subsets. Peripheral blood mononuclear cells (PBMC) from all goats were cultured in the presence of M. bovis tuberculin (PPD-B). (A,B) Singlet lymphocytes were identified based on the degree of cellular differentiation determined by forward scatter (FSC) and side scatter (SCC). (C) Representative frequencies of the CD4/CD45RO cell populations. (D) Representative frequencies of intracellular IFN-γ staining of cells gated from prelabelled CD4+CD45RO+.
From: Arrieta-Villegas C, Vidal E, Martín M, Verdés J, Moll X, Espada Y, Singh M, Villarreal-Ramos B, Domingo M, Pérez de Val B.
Immunogenicity and Protection against Mycobacterium caprae. Challenge in Goats Vaccinated with BCG and Revaccinated after One Year.
Vaccines (Basel). 2020 Dec 10;8(4):751.
doi: 10.3390/vaccines8040751..
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Bovine CD45RO:Alexa Fluor® 647
Mouse anti Bovine CD45RO
Mouse anti Bovine CD45RO:RPE
- Product Type
- Monoclonal Antibody
- Clone
- IL-A116
- Isotype
- IgG3
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA2434A647 | F | 100 Tests/1ml |
|
|
| MCA2434GA | F IP | 0.1 mg |
|
|
| MCA2434PE | F | 100 Tests |
|
Mouse anti Bovine CD45RO, clone IL-A116 recognises the bovine homologue of the human CD45RO cell surface antigen.
CD45, also known as Leucocyte Common Antigen or LCA, occurs in a number of isoforms, clone IL-A116 is specific for the low molecular weight isoform termed CD45RO, the isoform associated with expression on memory T-cells. Bovine CD45RO is expressed by monocytes, granulocytes and subsets of thymocytes, CD4+ T cells and CD8+ T cells. Studies utilizing clone IL-A116 have demonstrated that the percentage of CD45RO+ CD8+ T cells increase from approximately 5% in neonatal calves to approximately 35% in adult cattle over the age of 5 years (Hogg et al. 2011). It has been demonstrated that mouse anti bovine CD45RO, clone IL-A116 immunoprecipitates a molecule of ~180 kDa (Bembridge et al. 1995) which is analogus to the molecular weight of human and mouse CD45RO.
Mouse anti Bovine CD45RO, clone IL-A116 has been demonstrated to recognise the CD45RO cell surface antigen by flow cytometry in both European cattle, Bos taurus, and in Zebu, B.indicus (Bembridge et al. 1995).
CD45, also known as Leucocyte Common Antigen or LCA, occurs in a number of isoforms, clone IL-A116 is specific for the low molecular weight isoform termed CD45RO, the isoform associated with expression on memory T-cells. Bovine CD45RO is expressed by monocytes, granulocytes and subsets of thymocytes, CD4+ T cells and CD8+ T cells. Studies utilizing clone IL-A116 have demonstrated that the percentage of CD45RO+ CD8+ T cells increase from approximately 5% in neonatal calves to approximately 35% in adult cattle over the age of 5 years (Hogg et al. 2011). It has been demonstrated that mouse anti bovine CD45RO, clone IL-A116 immunoprecipitates a molecule of ~180 kDa (Bembridge et al. 1995) which is analogus to the molecular weight of human and mouse CD45RO.
Mouse anti Bovine CD45RO, clone IL-A116 has been demonstrated to recognise the CD45RO cell surface antigen by flow cytometry in both European cattle, Bos taurus, and in Zebu, B.indicus (Bembridge et al. 1995).
Product Details
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Goat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor 647 - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose - Carrier Free
- Yes
- Immunogen
- Bovine peripheral blood monouclear cells.
- Approx. Protein Concentrations
- Ig concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line.
Storage Information
- Storage
- Store at +4°C or at -20°C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC.
Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD45RO antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | 1/10 | 1/50 | |
| Immunoprecipitation | |||
| Flow Cytometry | Neat | 1/10 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for CD45RO antibody
-
Bembridge, G.P. et al. (1995) CD45RO expression on bovine T cells: relation to biological function.
Immunology. 86 (4): 537-44. -
Hogg, A.E. et al. (2011) Characterization of age-related changes in bovine CD8+ T-cells.
Vet Immunol Immunopathol. 140 (1-2): 47-54. -
Whelan, A.O. et al. (2011) Development of an antibody to bovine IL-2 reveals multifunctional CD4 T(EM) cells in cattle naturally infected with bovine tuberculosis.
PLoS One. 6 (12): e29194. -
Howard, C.J. & Naessens, J. (1993) Summary of workshop findings for cattle (tables 1 and 2).
Vet Immunol Immunopathol. 39 (1-3): 25-47. -
Sopp, P. & Howard, C.J. (2001) IFN gamma and IL-4 production by CD4, CD8 and WC1 gamma delta TCR(+) T cells from cattle lymph nodes and blood.
Vet Immunol Immunopathol. 81 (1-2): 85-96. -
Mcinnes, E. et al. (1999) Phenotypic analysis of local cellular responses in calves infected with bovine respiratory syncytial virus.
Immunology. 96 (3): 396-403. -
Bembridge, G.P. et al. (1993) Comparison of monoclonal antibodies with potential specificity for restricted isoforms of the leukocyte common antigen (CD45R).
Vet Immunol Immunopathol. 39 (1-3): 129-36. -
Naessens, J. et al. (1993) Cross-reactivity of workshop antibodies with cells from domestic and wild ruminants.
Vet Immunol Immunopathol. 39 (1-3): 283-90. -
Schmidt, N. et al. (2018) Decreased STEC shedding by cattle following passive and active vaccination based on recombinant Escherichia coli Shiga toxoids.
Vet Res. 49 (1): 28. -
Arrieta-Villegas, C. et al. (2020) Immunogenicity and Protection against Mycobacterium caprae Challenge in Goats Vaccinated with BCG and Revaccinated after One Year.
Vaccines (Basel). 8 (4): 751.
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