CD4 antibody | CC30

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Mouse anti Bovine CD4

Product Type
Monoclonal Antibody
Clone
CC30
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA834GA C F IP P* 0.1 mg
Mouse anti Bovine CD4 antibody, clone CC30 recognizes a ~50 kDa transmembrane molecule considered to be the bovine homologue of human CD4. The phenotype, tissue distribution and function of T-cells expressing the bovine CD4 antigen are similar to those in other species. However, expression on macrophages has not yet been detected.

Mouse anti Bovine CD4, clone CC30 has successfully been used for immunohistochemical localization of CD4 on paraffin embedded material using zinc salt fixation (Cantón et al. 2013). Additionally, clone CC30 has been reported as being suitable for use on formal dichromate (FD5) fixed paraffin embedded tissue with amplification and antigen retrieval techniques (Gutierrez et al. 1999).

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bison
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Bovine thymocytes.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from an immunised mouse were fused with cells of the mouse NS1 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
A7YY52 Related reagents
Regulatory
For research purposes only

Applications of CD4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/200
Immunohistology - Frozen 1/100
Immunohistology - Paraffin 1
Immunoprecipitation
  1. 1
    Clone CC30 has been reported as being suitable for use on formal dichromate (FD5) fixed paraffin embedded tissue with amplification and antigen retrieval techniques, see Gutierrez et al. for details.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA928 100 Tests F

Application Based External Images

Flow Cytometry

Immunofluorescence

Immunohistology - Paraffin

Product Specific References

References for CD4 antibody

  1. Bensaid, A. & Hadam, M. (1991) Individual antigens of cattle. Bovine CD4 (BoCD4).
    Vet Immunol Immunopathol. 27 (1-3): 51-4.
  2. Eskra, L. et al. (1991) Effect of monoclonal antibodies on in vitro function of T-cell subsets.
    Vet Immunol Immunopathol. 27 (1-3): 215-22.
  3. Gutierrez, M. et al. (1999) The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques.
    Vet Immunol Immunopathol. 71 (3-4): 321-34.
  4. Winkler, M.T. et al. (1999) Bovine herpesvirus 1 can infect CD4(+) T lymphocytes and induce programmed cell death during acute infection of cattle.
    J Virol. 73 (10): 8657-68.
  5. Winkler, M.T. et al. (2000) Persistence and reactivation of bovine herpesvirus 1 in the tonsils of latently infected calves.
    J Virol. 74 (11): 5337-46.
  6. Riondato, F. et al. (2008) Effects of road transportation on lymphocyte subsets in calves
    Vet J. 175: 364-8.
  7. Collins, R.A. et al. (1999) Bovine interleukin-12 and modulation of IFNgamma production.
    Vet Immunol Immunopathol. 68: 193-207.
  8. Liebana, E. et al. (2007) Distribution and activation of T-lymphocyte subsets in tuberculous bovine lymph-node granulomas.
    Vet Pathol. 44: 366-72.
  9. Sühwold, A. et al. (2010) T cell reactions of Eimeria bovis primary and challenge-infected calves.
    Parasitol Res. 106: 595-605.
  10. Rhodes, S.G. et al. (2000) Bovine tuberculosis: immune responses in the peripheral blood and at the site of active disease.
    Immunology. 99: 195-202.
  11. Machugh, N.D. et al. (1997) Identification of two distinct subsets of bovine gamma delta T cells with unique cell surface phenotype and tissue distribution.
    Immunology. 92: 340-5.
  12. Childerstone, A.J. et al. (1999) Demonstration of bovine CD8+ T-cell responses to foot-and-mouth disease virus.
    J Gen Virol. 80: 663-9.
  13. Rhodes, S.G. et al. (2001) Antigen recognition and immunomodulation by gamma delta T cells in bovine tuberculosis.
    J Immunol. 166: 5604-10.
  14. Knowles, G. et al. (1996) Phenotypical characterization of lymphocytes infiltrating regressing papillomas.
    J Virol. 70: 8451-8.
  15. Smyth, A.J. et al. (2001) In vitro responsiveness of gammadelta T cells from Mycobacterium bovis-infected cattle to mycobacterial antigens: predominant involvement of WC1(+) cells.
    Infect Immun. 69: 89-96.
  16. Hein, W.R. and Dudler, L. (1997) TCR gamma delta+ cells are prominent in normal bovine skin and express a diverse repertoire of antigen receptors.
    Immunology. 91: 58-64.
  17. Juleff, N. et al. (2009) Foot-and-mouth disease virus can induce a specific and rapid CD4+ T-cell-independent neutralizing and isotype class-switched antibody response in naïve cattle.
    J Virol. 83: 3626-36.
  18. Hope, J.C. et al. (2005) Exposure to Mycobacterium avium induces low-level protection from Mycobacterium bovis infection but compromises diagnosis of disease in cattle.
    Clin Exp Immunol. 141: 432-9.
  19. Sopp, P. et al. (2006) Flow cytometric detection of gamma interferon can effectively discriminate Mycobacterium bovis BCG-vaccinated cattle from M. bovis-infected cattle.
    Clin Vaccine Immunol. 13: 1343-8.
  20. Sacchini, F. et al. (2011) A minor role of CD4+ T lymphocytes in the control of a primary infection of cattle with Mycoplasma mycoides subsp. mycoides.
    Vet Res. 42: 77.
  21. Skyberg, J.A. et al. (2011) Murine and bovine γδ T cells enhance innate immunity against Brucella abortus infections.
    PLoS One. 6(7): e21978.
  22. Cantón, G.J. et al. (2013) Phenotypic characterisation of the cellular immune infiltrate in placentas of cattle following experimental inoculation with Neospora caninum in late gestation.
    Vet Res. 44: 60.
  23. Reid, E. et al. (2011) Bovine plasmacytoid dendritic cells are the major source of type I interferon in response to foot-and-mouth disease virus in vitro and in vivo.
    J Virol. 85: 4297-308.
  24. Arranz-Solís D et al. (2016) Systemic and local immune responses in sheep after Neospora caninum experimental infection at early, mid and late gestation.
    Vet Res. 47 (1): 2.
  25. Clapp, B. et al. (2011) DNA vaccination of bison to brucellar antigens elicits elevated antibody and IFN-γ responses.
    J Wildl Dis. 47 (3): 501-10.
  26. Salman S et al. (2013) Colostrum and milk selenium, antioxidative capacity and immune status of dairy cows fed sodium selenite or selenium yeast.
    Arch Anim Nutr. 67 (1): 48-61.
  27. Aranday-Cortes, E. et al. (2013) Upregulation of IL-17A, CXCL9 and CXCL10 in early-stage granulomas induced by Mycobacterium bovis in cattle.
    Transbound Emerg Dis. 60 (6): 525-37.
  28. Pirson, C. et al. (2015) Highly purified mycobacterial phosphatidylinositol mannosides drive cell-mediated responses and activate NKT cells in cattle.
    Clin Vaccine Immunol. 22 (2): 178-84.
  29. Fuertes, M. et al. (2015) Immunohistochemical study and mRNA cytokine profile of the local immune response in cattle naturally infected with Calicophoron daubneyi.
    Vet Parasitol. 214 (1-2): 178-83.