CD4 antibody | CC30
Mouse anti Bovine CD4 antibody, clone CC30 (MCA834GA )used to demonstrate the presence of T helper cells in ovine placenta during gestation by immunohistochemistry on cryostat sections.
Image caption:
Comparison of the immunohistochemical labelling of T inflammatory cells and parasite antigen in the placenta. This panel compares the distribution and frequency of T lymphocytes (CD3, CD4 and CD8 antigens) and parasite antigen immunohistochemically labelled in samples from the three groups. Pictures showing the labelling of T cells (first three rows) were taken at 75× and those of the parasite antigen (last row) were taken at 150×. F: foetal mesenchyme, M: maternal endometrial stalk.
From: Arranz-Solís D, Benavides J, Regidor-Cerrillo J, Horcajo P, Castaño P, Del Carmen Ferreras M, Jiménez-Pelayo L, Collantes-Fernández E, Ferre I, Hemphill A, Pérez V, Ortega-Mora LM.
Systemic and local immune responses in sheep after Neospora caninum experimental infection at early, mid and late gestation.
Vet Res. 2016 Jan 6;47(1):2.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Bovine CD4 antibody, clone CC30 (MCA834GA) used for the detection of CD4+ve helper T cells by immunohistochemistry on formalin fixed, paraffin embedded bovine placental sections.
Image caption:
Examples of different immune cell infiltration in placentomes from N. caninum-inoculated animals. (a) Severe infiltration of macrophages in the base of maternal caruncle of a N. caninum inoculated cow culled 14 dpi. Counterstained with haematoxylin. Original magnification 100×. (b) Mild aggregate of T helper cells (CD4+) surrounding a blood vessel in maternal caruncle of a placentome collected from an inoculated cow culled 28 dpi. Counterstained with haematoxylin. Original magnification 200×. (c) Minimal infiltration of cytotoxic T-cells (CD8+) in a necrotic focus in maternal caruncle of a N. caninum challenged cow culled 28 dpi. Counterstained with haematoxylin. Original magnification 200×. (d) Mild aggregate of γδ T-cells in the base of maternal caruncle of an inoculated cow culled 14 dpi. Counterstained with haematoxylin. Original magnification 200×. (e) Rare presence of NK-cells in connective tissue in the maternal caruncle surrounding necrotic and mineralized foetal villi, observed in a placenta of an inoculated cow culled 28 dpi. Counterstained with haematoxylin. Original magnification 200×. (f) Rare presence of CD79αcy+ cells aligned with endometrial epithelial cells in the maternal caruncle and in some foetal villi, observed in the placentome of a N. caninum inoculated cow culled 28 dpi. Original magnification 400×. Counterstained with haematoxylin.
From: Cantón GJ, Katzer F, Benavides-Silván J, Maley SW, Palarea-Albaladejo J, Pang Y, Smith S, Bartley PM, Rocchi M, Innes EA, Chianini F.
Phenotypic characterisation of the cellular immune infiltrate in placentas of cattle following experimental inoculation with Neospora caninum in late gestation.
Vet Res. 2013 Jul 22;44:60.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Bovine CD4 antibody, clone CC63 (MCA834GA) used for the identification of CD4 expressing lymphocytes in bovine tissue sections by immunohistochemistry on formalin fixed, paraffin sections.
Image caption:
Immunohistochemical staining of CD4 + T cells of the Bovine mammary tissue using paraffin-embedded sections (A–C): (A) Showing the low immunoreactivity of T lymphocytes (arrow) in the interlobular connective tissue with the monoclonal antibody specific for CD4+ T cells in Bovine mammary tissue infected with BPIV-3. (B) The intermediate immunoreactivity of CD4 + T cells (arrow) in the interalveolar connective tissue with the monoclonal antibody specific for CD4+ T cells in Bovine mammary tissue infected with BoHV-1. (C) The high reactivity distributional of CD4 + T lymphocytes (arrow) in the interalveolar connective tissue with the monoclonal antibody specific for CD4+ T cells in Bovine mammary tissue infected with BoHV-1 and BPIV-3. IHC. Bar, 20μm.
From: Çomakli S, Özdemir S. Comparative Evaluation of the Immune Responses in Cattle Mammary Tissues Naturally Infected with Bovine Parainfluenza Virus Type 3 and Bovine Alphaherpesvirus-1. Pathogens. 2019;8(1). pii: E26.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Bovine CD4 antibody, clone CC30 (MCA834GA) used to evaluate CD4 expression in bovine granulomatous lesions by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunolabeling for CD4+ cells (ABC IHC stain, DAB brown chromogen). (A) Comparison of CD4+ cell distribution in different granuloma stages of BCG vaccinated (Vac, vaccinated) and non-vaccinated (Con, control) calves. (B) Stage I granuloma from non-vaccinated calves, showing stained CD4+ cells (arrows). (C) Stage I granuloma from BCG vaccinated calf showing more CD4+ cells (arrows). (D) Stage IV granuloma from non-vaccinated calf showing stained CD4+ cells (arrows). (E) Stage IV granuloma from BCG vaccinated calf showing stained CD4+ cells (arrows). Bar equals 50μm in (B–E). n, necrotic core.
From: Sirak A, Tulu B, Bayissa B, Gumi B, Berg S, Salguero FJ, Ameni G; ETHICOBOTS Consortium.
Cellular and Cytokine Responses in Lymph Node Granulomas of Bacillus Calmette Guérin (BCG)-Vaccinated and Non-vaccinated Cross-Breed Calves Naturally Infected With Mycobacterium bovis.
Front Vet Sci. 2021 Sep 16;8:698800.
doi: 10.3389/fvets.2021.698800.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Bovine CD4
- Product Type
- Monoclonal Antibody
- Clone
- CC30
- Isotype
- IgG1
- Specificity
- CD4
Antibody Quality and Performance Guarantee
| Mouse anti Bovine CD4 antibody, clone CC30 recognizes a ~50 kDa transmembrane molecule considered to be the bovine homologue of human CD4. The phenotype, tissue distribution and function of T-cells expressing the bovine CD4 antigen are similar to those in other species. However, expression on macrophages has not yet been detected. Mouse anti Bovine CD4, clone CC30 has successfully been used for immunohistochemical localization of CD4 on paraffin embedded material using zinc salt fixation (Cantón et al. 2013). Additionally, clone CC30 has been reported as being suitable for use on formal dichromate (FD5) fixed paraffin embedded tissue with amplification and antigen retrieval techniques (Gutierrez et al. 1999). |
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bison - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Bovine thymocytes.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from an immunized mouse were fused with cells of the mouse NS1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/200 |
| Immunohistology - Frozen | |
1/100 | |
| Immunohistology - Paraffin 1 | |
||
| Immunoprecipitation | |
- 1
Clone CC30 has been reported as being suitable for use on formal dichromate (FD5) fixed paraffin embedded tissue with amplification and antigen retrieval techniques, see Gutierrez et al. for details.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for CD4 antibody
-
Bensaid, A. & Hadam, M. (1991) Individual antigens of cattle. Bovine CD4 (BoCD4).
Vet Immunol Immunopathol. 27 (1-3): 51-4. -
Eskra, L. et al. (1991) Effect of monoclonal antibodies on in vitro. function of T-cell subsets.
Vet Immunol Immunopathol. 27 (1-3): 215-22. -
Gutierrez, M. et al. (1999) The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques.
Vet Immunol Immunopathol. 71 (3-4): 321-34. -
Winkler, M.T. et al. (1999) Bovine herpesvirus 1 can infect CD4(+) T lymphocytes and induce programmed cell death during acute infection of cattle.
J Virol. 73 (10): 8657-68. -
Winkler, M.T. et al. (2000) Persistence and reactivation of bovine herpesvirus 1 in the tonsils of latently infected calves.
J Virol. 74 (11): 5337-46. -
Riondato, F. et al. (2008) Effects of road transportation on lymphocyte subsets in calves
Vet J. 175: 364-8. -
Collins, R.A. et al. (1999) Bovine interleukin-12 and modulation of IFNgamma production.
Vet Immunol Immunopathol. 68: 193-207. -
Liebana, E. et al. (2007) Distribution and activation of T-lymphocyte subsets in tuberculous bovine lymph-node granulomas.
Vet Pathol. 44: 366-72.
View The Latest Product References
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Sühwold, A. et al. (2010) T cell reactions of Eimeria bovis primary and challenge-infected calves.
Parasitol Res. 106: 595-605. -
Rhodes, S.G. et al. (2000) Bovine tuberculosis: immune responses in the peripheral blood and at the site of active disease.
Immunology. 99: 195-202. -
Machugh, N.D. et al. (1997) Identification of two distinct subsets of bovine gamma delta T cells with unique cell surface phenotype and tissue distribution.
Immunology. 92: 340-5. -
Childerstone, A.J. et al. (1999) Demonstration of bovine CD8+ T-cell responses to foot-and-mouth disease virus.
J Gen Virol. 80: 663-9. -
Rhodes, S.G. et al. (2001) Antigen recognition and immunomodulation by gamma delta T cells in bovine tuberculosis.
J Immunol. 166: 5604-10. -
Knowles, G. et al. (1996) Phenotypical characterization of lymphocytes infiltrating regressing papillomas.
J Virol. 70: 8451-8. -
Smyth, A.J. et al. (2001) In vitro responsiveness of gammadelta T cells from Mycobacterium bovis-infected cattle to mycobacterial antigens: predominant involvement of WC1(+) cells.
Infect Immun. 69: 89-96. -
Hein, W.R. and Dudler, L. (1997) TCR gamma delta+ cells are prominent in normal bovine skin and express a diverse repertoire of antigen receptors.
Immunology. 91: 58-64. -
Juleff, N. et al. (2009) Foot-and-mouth disease virus can induce a specific and rapid CD4+ T-cell-independent neutralizing and isotype class-switched antibody response in naïve cattle.
J Virol. 83: 3626-36. -
Hope, J.C. et al. (2005) Exposure to Mycobacterium avium induces low-level protection from Mycobacterium bovis infection but compromises diagnosis of disease in cattle.
Clin Exp Immunol. 141: 432-9. -
Sopp, P. et al. (2006) Flow cytometric detection of gamma interferon can effectively discriminate Mycobacterium bovis BCG-vaccinated cattle from M. bovis-infected cattle.
Clin Vaccine Immunol. 13: 1343-8. -
Sacchini, F. et al. (2011) A minor role of CD4+ T lymphocytes in the control of a primary infection of cattle with Mycoplasma mycoides subsp. mycoides.
Vet Res. 42: 77. -
Skyberg, J.A. et al. (2011) Murine and bovine γδ T cells enhance innate immunity against Brucella abortus infections.
PLoS One. 6(7): e21978. -
Cantón, G.J. et al. (2013) Phenotypic characterisation of the cellular immune infiltrate in placentas of cattle following experimental inoculation with Neospora caninum in late gestation.
Vet Res. 44: 60. -
Reid, E. et al. (2011) Bovine plasmacytoid dendritic cells are the major source of type I interferon in response to foot-and-mouth disease virus in vitro and in vivo.
J Virol. 85: 4297-308. -
Arranz-Solís D et al. (2016) Systemic and local immune responses in sheep after Neospora caninum experimental infection at early, mid and late gestation.
Vet Res. 47 (1): 2. -
Clapp, B. et al. (2011) DNA vaccination of bison to brucellar antigens elicits elevated antibody and IFN-γ responses.
J Wildl Dis. 47 (3): 501-10. -
Aranday-Cortes, E. et al. (2013) Upregulation of IL-17A, CXCL9 and CXCL10 in early-stage granulomas induced by Mycobacterium bovis in cattle.
Transbound Emerg Dis. 60 (6): 525-37. -
Pirson, C. et al. (2015) Highly purified mycobacterial phosphatidylinositol mannosides drive cell-mediated responses and activate NKT cells in cattle.
Clin Vaccine Immunol. 22 (2): 178-84. -
Fuertes, M. et al. (2015) Immunohistochemical study and mRNA cytokine profile of the local immune response in cattle naturally infected with Calicophoron daubneyi.
Vet Parasitol. 214 (1-2): 178-83. -
Çomakli, S. & Özdemir, S. (2019) Comparative Evaluation of the Immune Responses in Cattle Mammary Tissues Naturally Infected with Bovine Parainfluenza Virus Type 3 and Bovine Alphaherpesvirus-1.
Pathogens.8 (1): 26. -
Bozkurt, Y.A. et al. (2019) The architecture of the lymph nodes in the abdominal and thoracic cavities of wild boar
Ind J Anim Res. 53 609-15. -
Hecker, Y.P. et al. (2015) Cell mediated immune responses in the placenta following challenge of vaccinated pregnant heifers with Neospora caninum.
Vet Parasitol. 214 (3-4): 247-54. -
Cunha, P. et al. (2019) Expansion, isolation and first characterization of bovine Th17 lymphocytes.
Sci Rep. 9 (1): 16115. -
Sirak, A. et al. (2021) Cellular and Cytokine Responses in Lymph Node Granulomas of Bacillus Calmette Guérin (BCG)-Vaccinated and Non-vaccinated Cross-Breed Calves Naturally Infected With Mycobacterium bovis.
Front Vet Sci. 8: 698800. -
Korbonits, L. et al. (2022) Mycobacterium avium subsp. paratuberculosis Infected Cows Reveal Divergent Immune Response in Bovine Peripheral Blood Derived Lymphocyte Proteome.
Metabolites. 12 (10): 924. -
Andrés, S. et al. (2024) Essential oil supplementation in milk replacers: short- and long-term impacts on feed efficiency, the faecal microbiota and the plasma metabolome in dairy calves.
J Dev Orig Health Dis. : 1-11.
- UniProt
- A7YY52
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