CD21 antibody | CC21
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Mouse anti Bovine CD21
- Product Type
- Monoclonal Antibody
|Mouse anti Bovine CD21 monoclonal antibody, clone CC21 recognizes the bovine CD21 cell surface antigen, a ~145 kDa single pass type I membrane glycoprotein containing multiple sushi domains. CD21 is also known as complement receptor type 2. In cattle CD21 expression is restricted to B lymphocytes (Naessens et al. 1990). CD21 may be expressed on B cells as either a long or a short form (Pringle et al. 2012)
Mouse anti bovine CD21, clone CC21 has been used to demonstrate the co-expression of CD21 with
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Goat Sheep Red deer Mule deer
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSI myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
References for CD21 antibody
Naessens, J. et al. (1990) Characterization of a bovine leucocyte differentiation antigen of 145,000 Mw restricted to B lymphocytes.
Immunology 69: 525-30.
Howard, C.J. et al. (1991) Summary of workshop findings for leukocyte antigens of cattle.
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Sopp, P. & Howard, C.J. (2001) IFN gamma and IL-4 production by CD4, CD8 and WC1 gamma delta TCR(+) T cells from cattle lymph nodes and blood.
Vet Immunol Immunopathol. 81 (1-2): 85-96.
Sigurdson, C.J. et al. (2002) PrP(CWD) lymphoid cell targets in early and advanced chronic wasting disease of mule deer.
J Gen Virol. 83: 2617-28.
Kruger, E.F. et al. (2003) Bovine monocytes induce immunoglobulin production in peripheral blood B lymphocytes.
Dev Comp Immunol. 27 (10): 889-97.
Newland, A. et al. (2004) Ovine dendritic cells transduced with an adenoviral CTLA4eEGFP fusion protein construct induce hyporesponsiveness to allostimulation.
Immunology. 113: 310-7.
Halliday, S. et al. (2005) Expression of PrPC on cellular components of sheep blood.
J Gen Virol. 86 (Pt 5): 1571-9.
Brackenbury, L.S. et al. (2005) Identification of a cell population that produces alpha/beta interferon in vitro and in vivo in response to noncytopathic bovine viral diarrhea virus.
J Virol. 79: 7738-44.
View The Latest Product References
Weiss, D.J. et al. (2006) Mucosal immune response in cattle with subclinical Johne's disease.
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Richt, J.A. et al. (2007) Production of cattle lacking prion protein.
Nat Biotechnol. 25: 132-8.
Lwin, S. et al. (2009) Immune cell types involved in early uptake and transport of recombinant mouse prion protein in Peyer's patches of calves.
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Ekman, A. et al. (2010) B-cell development in bovine fetuses proceeds via a pre-B like cell in bone marrow and lymph nodes.
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Edwards, J.C. et al. (2010) PrP(Sc) is associated with B cells in the blood of scrapie-infected sheep.
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Kiku, Y. et al. (2010) Decrease in bovine CD14 positive cells in colostrum is associated with the incidence of mastitis after calving.
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Chattha, K.S. et al. (2010) Immunohistochemical investigation of cells expressing CD21, membrane IgM, CD32 and a follicular dendritic cell marker in the lymphoid tissues of neonatal calves.
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Brujeni, G.N. et al. (2010) Bovine immunodeficiency virus and bovine leukemia virus and their mixed infection in Iranian Holstein cattle.
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Booth, J.S. et al. (2010) Co-stimulation with TLR7/8 and TLR9 agonists induce down-regulation of innate immune responses in sheep blood mononuclear and B cells.
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Breugelmans, S. et al. (2011) Differences between the ovine tonsils based on an immunohistochemical quantification of the lymphocyte subpopulations.
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Breugelmans, S. et al. (2011) Immunoassay of lymphocyte subsets in ovine palatine tonsils.
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Summers, C. et al. (2012) The distribution of immune cells in the lungs of classical and atypical ovine pulmonary adenocarcinoma.
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Dagleish, M.P.et al. (2012) Immunophenotype of cells within cervine rectoanal mucosa-associated lymphoid tissue and mesenteric lymph nodes.
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Nikbakht Brujeni, G. et al. (2016) Association of BoLA-DRB3.2 Alleles with BLV Infection Profiles (Persistent Lymphocytosis/Lymphosarcoma) and Lymphocyte Subsets in Iranian Holstein Cattle.
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De Matteis G et al. (2016) Evaluation of leptin receptor expression on buffalo leukocytes.
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