CD21 antibody | CC21
Mouse anti Bovine CD21 monoclonal antibody, clone CC21 (MCA1424GA) used to identify ovine CD21 expressing cells in heart tissue by immunofluorescence on cryostat sections.
Image caption:
Immunofluorescence examination of explanted heart valves. CD45‐positive cells could be found within (A) allogeneic and (B) xenogeneic explants. C, Allogeneic explants exhibited the lowest number of CD45‐positive cells, whereas xenogeneic (xeno) SDS/SD‐treated explants exhibited the highest number, about 40% of all invading cells. High numbers of CD11b‐positive cells were present in all (D) allogeneic and (E) xenogeneic explants; however, (F) no significant differences were found. G, In allogeneic explants, CD3‐positive T cells were almost absent, but in (H) xenogeneic explants, a low number of CD3‐positive cells could be found. I, Enzyme‐treated valves tended to contain less CD3‐positive cells as untreated explants. J, In allogeneic as well (K) xenogeneic explants, (L) only few CD21‐positive B‐cells could be found. C, F, I, L, Every dot represents one individual animal included in the analysis. For each PHV specimen, at least two sections obtained from different areas have been analyzed, counting 11 HPFs per section. All scale bars represent 100 μm.
From: Ramm R, Goecke T, Theodoridis K, Hoeffler K, Sarikouch S, Findeisen K, Ciubotaru A, Cebotari S, Tudorache I, Haverich A, Hilfiker A.
Decellularization combined with enzymatic removal of N-linked glycans and residual DNA reduces inflammatory response and improves performance of porcine xenogeneic pulmonary heart valves in an ovine in vivo model.
Xenotransplantation. 2020 Mar;27(2):e12571.
doi: 10.1111/xen.12571.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Phycoerythrin conjugated Mouse anti Bovine CD21 monoclonal antibody, clone CC21 (MCA1424PE) used to discriminate lymphocyte populations in bovine blood samples by flow cytometry.
Image caption:
Changes in T and B lymphocytes among PBMCs in Holstein and Jersey steers subjected to heat stress. Flow cytometry analysis to identify lymphocytes subset population. Heat stress caused reduction in lymphocyte populations in the PBMCs of both Holstein and Jersey steers. The lymphocytes comprised CD21+MHCII+ B cells. MT indicates moderate THI condition and HT indicates high THI condition. * = P <0.05, ** = P <0.01
From: Park DS, Gu BH, Park YJ, Joo SS, Lee SS, Kim SH, Kim ET, Kim DH, Lee SS, Lee SJ, Kim BW, Kim M.
Dynamic changes in blood immune cell composition and function in Holstein and Jersey steers in response to heat stress.
Cell Stress Chaperones. 2021 Jul;26(4):705-20.
doi: 10.1007/s12192-021-01216-2.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
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F IF C ResetMouse anti Bovine CD21
- Product Type
- Monoclonal Antibody
- Clone
- CC21
- Isotype
- IgG1
- Specificity
- CD21
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| Mouse anti Bovine CD21 monoclonal antibody, clone CC21 recognizes the bovine CD21 cell surface antigen, a ~145 kDa single pass type I membrane glycoprotein containing multiple sushi domains. CD21 is also known as complement receptor type 2. In cattle CD21 expression is restricted to B lymphocytes (Naessens et al. 1990). CD21 may be expressed on B cells as either a long or a short form (Pringle et al. 2012) Mouse anti bovine CD21, clone CC21 has been used to demonstrate the co-expression of CD21 with |
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Goat Sheep Red deer Mule deer - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSI myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/200 |
| Immunofluorescence | |
||
| Immunohistology - Frozen | |
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| Immunoprecipitation | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for CD21 antibody
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Newland, A. et al. (2004) Ovine dendritic cells transduced with an adenoviral CTLA4eEGFP fusion protein construct induce hyporesponsiveness to allostimulation.
Immunology. 113: 310-7. -
Richt, J.A. et al. (2007) Production of cattle lacking prion protein.
Nat Biotechnol. 25: 132-8. -
Howard, C.J. et al. (1991) Summary of workshop findings for leukocyte antigens of cattle.
Vet Immunol Immunopathol. 27 (1-3): 21-7. -
Naessens, J. et al. (1990) Characterization of a bovine leucocyte differentiation antigen of 145,000 Mw restricted to B lymphocytes.
Immunology 69: 525-30. -
Sopp, P. & Howard, C.J. (2001) IFN gamma and IL-4 production by CD4, CD8 and WC1 gamma delta TCR(+) T cells from cattle lymph nodes and blood.
Vet Immunol Immunopathol. 81 (1-2): 85-96. -
Lwin, S. et al. (2009) Immune cell types involved in early uptake and transport of recombinant mouse prion protein in Peyer's patches of calves.
Cell Tissue Res. 338: 343-54. -
Breugelmans, S. et al. (2011) Immunoassay of lymphocyte subsets in ovine palatine tonsils.
Acta Histochem. 113: 416-22. -
Halliday, S. et al. (2005) Expression of PrPC on cellular components of sheep blood.
J Gen Virol. 86 (Pt 5): 1571-9.
View The Latest Product References
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Brackenbury, L.S. et al. (2005) Identification of a cell population that produces alpha/beta interferon in vitro and in vivo in response to noncytopathic bovine viral diarrhea virus.
J Virol. 79: 7738-44. -
Breugelmans, S. et al. (2011) Differences between the ovine tonsils based on an immunohistochemical quantification of the lymphocyte subpopulations.
Comp Immunol Microbiol Infect Dis. 34: 217-25. -
Richt, J.A. et al. (2007) Production of cattle lacking prion protein.
Nat Biotechnol. 25: 132-8. -
Brujeni, G.N. et al. (2010) Bovine immunodeficiency virus and bovine leukemia virus and their mixed infection in Iranian Holstein cattle.
J Infect Dev Ctries. 4 (9): 576-9. -
Kiku, Y. et al. (2010) Decrease in bovine CD14 positive cells in colostrum is associated with the incidence of mastitis after calving.
Vet Res Commun. 34: 197-203. -
Pilla, R. et al. (2012) Long-term study of MRSA ST1, t127 mastitis in a dairy cow.
Vet Rec. 170: 312. -
Chattha, K.S. et al. (2010) Immunohistochemical investigation of cells expressing CD21, membrane IgM, CD32 and a follicular dendritic cell marker in the lymphoid tissues of neonatal calves.
Vet Immunol Immunopathol. 137: 284-90. -
Weiss, D.J. et al. (2006) Mucosal immune response in cattle with subclinical Johne's disease.
Vet Pathol. 43: 127-35. -
Summers, C. et al. (2012) The distribution of immune cells in the lungs of classical and atypical ovine pulmonary adenocarcinoma.
Vet Immunol Immunopathol. 146: 1-7. -
Sigurdson, C.J. et al. (2002) PrP(CWD) lymphoid cell targets in early and advanced chronic wasting disease of mule deer.
J Gen Virol. 83: 2617-28. -
Dagleish, M.P.et al. (2012) Immunophenotype of cells within cervine rectoanal mucosa-associated lymphoid tissue and mesenteric lymph nodes.
J Comp Pathol. 146: 365-71. -
Edwards, J.C. et al. (2010) PrP(Sc) is associated with B cells in the blood of scrapie-infected sheep.
Virology. 405: 110-9. -
Brodzki, P. et al. (2014) Phenotyping of leukocytes and granulocyte and monocyte phagocytic activity in the peripheral blood and uterus of cows with endometritis.
Theriogenology. 82: 403-10. -
Meganck, V. et al. (2014) Development of a method for isolating bovine colostrum mononuclear leukocytes for phenotyping and functional studies.
Vet J. 200: 294-8. -
Booth, J.S. et al. (2010) Co-stimulation with TLR7/8 and TLR9 agonists induce down-regulation of innate immune responses in sheep blood mononuclear and B cells.
Dev Comp Immunol. 34 (5): 572-8. -
Nikbakht Brujeni, G. et al. (2016) Association of BoLA-DRB3.2 Alleles with BLV Infection Profiles (Persistent Lymphocytosis/Lymphosarcoma) and Lymphocyte Subsets in Iranian Holstein Cattle.
Biochem Genet. 54 (2): 194-207. -
Kruger, E.F. et al. (2003) Bovine monocytes induce immunoglobulin production in peripheral blood B lymphocytes.
Dev Comp Immunol. 27 (10): 889-97. -
De Matteis G et al. (2016) Evaluation of leptin receptor expression on buffalo leukocytes.
Vet Immunol Immunopathol. 177: 16-23. -
Ekman, A. et al. (2010) B-cell development in bovine fetuses proceeds via a pre-B like cell in bone marrow and lymph nodes.
Dev Comp Immunol. 34 (8): 896-903. -
Silva, A.P. et al. (2015) Encapsulated Brucella ovis Lacking a Putative ATP-Binding Cassette Transporter (ΔabcBA) Protects against Wild Type Brucella ovis in Rams.
PLoS One. 10 (8): e0136865. -
Jimbo, S. et al. (2019) Natural and inducible regulatory B cells are widely distributed in ovine lymphoid tissues.
Vet Immunol Immunopathol. 211: 44-8. -
Brodzki, P. et al. (2019) Selected leukocyte subpopulations in peripheral blood and uterine washings in cows before and after intrauterine administration of cefapirin and methisoprinol.
Anim Sci J. Nov 06 [Epub ahead of print]. -
Souza, F.N. et al. (2020) Lymphocyte proliferative responses in dairy cows supplemented with an immunomodulatory feed additive and administered polyvalent vaccination.
Arquivo Brasileiro de Medicina Veterinária e Zootecnia. 72 (6): 2397-401. -
Gondaira, S. et al. (2020) Immunosuppression in Cows following Intramammary Infusion of Mycoplasma bovis.
Infect Immun. 88 (3) Feb 20 [Epub ahead of print]. -
Khosa, S. et al. (2020) Bovine Adenovirus-3 Tropism for Bovine Leukocyte Sub-Populations.
Viruses. 12 (12) Dec 12 [Epub ahead of print]. -
Ramm, R. et al. (2020) Decellularization combined with enzymatic removal of N-linked glycans and residual DNA reduces inflammatory response and improves performance of porcine xenogeneic pulmonary heart valves in an ovine in vivo model.
Xenotransplantation. 27 (2): e12571. -
Ramos, A. et al. (2018) Melatonin enhances responsiveness to Dichelobacter nodosus vaccine in sheep and increases peripheral blood CD4 T lymphocytes and IgG-expressing B lymphocytes.
Vet Immunol Immunopathol. 206: 1-8. -
Okino, C.H. et al. (2020) A polymorphic CD4 epitope related to increased susceptibility to Babesia bovis. in Canchim calves.
Vet Immunol Immunopathol. 230: 110132. -
Park, D.S. et al. (2021) Dynamic changes in blood immune cell composition and function in Holstein and Jersey steers in response to heat stress.
Cell Stress Chaperones. 26 (4): 705-20. -
Radley, G. et al. (2020) In Vitro. Benchmarking Study of Ventricular Assist Devices in Current Clinical Use.
J Card Fail. 26 (1): 70-79. -
Colombatti Olivieri, M.A. et al. (2021) Evaluation of a virulent strain of Mycobacterium avium. subsp. paratuberculosis. used as a heat-killed vaccine.
Vaccine. NNov 10;S0264-410X(21)01433-X. -
Casaro, S. et al. (2022) Flow cytometry panels for immunophenotyping dairy cattle peripheral blood leukocytes
Vet Immunol Immunopathol. 248: 110417. -
Okino, C.H. et al. (2022) CD4 bovine gene: Differential polymorphisms among cattle breeds and a new tool for rapid identification.
Vet Immunol Immunopathol. 251: 110462.
- Synonyms
- CR2
- RRID
- AB_11152606
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