CD21 antibody | CC21

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Anti Bovine CD21 Antibody, clone CC21 thumbnail image 1

Anti Bovine CD21 Antibody, clone CC21 thumbnail image 2

Anti Bovine CD21 Antibody, clone CC21 gallery image 1 — Figure A. RPE conjugated Mouse anti Bovine CD8b (MCA1654PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated Mouse anti Bovine CD8b (MCA1654PE) and FITC conjugated Mouse anti Bovine CD21 (**MCA1424F**). All experiments performed on red cell lysed bovine blood gated on lymphocytes in the presence of 10% bovine serum. Data acquired on the ZE5™ Cell Analyzer.

Anti Bovine CD21 Antibody, clone CC21 gallery image 2 — Figure A. RPE conjugated Mouse anti Bovine CD8 (MCA837PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated Mouse anti Bovine CD8 (MCA837PE) and FITC conjugated Mouse anti Bovine CD21 (**MCA1424F**). All experiments performed on red cell lysed bovine blood gated on lymphocytes in the presence of 10% bovine serum. Data acquired on the ZE5™ Cell Analyzer.

Mouse anti Bovine CD21:FITC

Mouse anti Bovine CD21

Mouse anti Bovine CD21:RPE

Product Type: Monoclonal Antibody
Clone: CC21
Isotype: IgG1

Product Code	Applications	Pack Size
MCA1424F	F	0.1 mg
MCA1424GA	C F IF IP	0.1 mg
MCA1424PE	F	100 Tests

Mouse anti Bovine CD21 monoclonal antibody, clone CC21 recognizes the bovine CD21 cell surface antigen, a ~145 kDa single pass type I membrane glycoprotein containing multiple sushi domains. CD21 is also known as complement receptor type 2. In cattle CD21 expression is restricted to B lymphocytes (Naessens et al. 1990). CD21 may be expressed on B cells as either a long or a short form (Pringle et al. 2012)

Mouse anti bovine CD21, clone CC21 has been used to demonstrate the co-expression of CD21 with PrP^c on B cells of scrapie infected sheep (Halliday et al. 2005).

Product Details

Target Species

Bovine

Species Cross-Reactivity

Target Species	Cross Reactivity
Goat
Sheep
Red deer
Mule deer

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Product Form

Purified IgG - liquid

Product Form

Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized

Reconstitution

Reconstitute with 1ml distilled water.

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Preservative Stabilisers

0.09% Sodium Azide
1% Bovine Serum Albumin
5% Sucrose

Carrier Free

Yes

Approx. Protein Concentrations

IgG concentration 0.1 mg/ml

Approx. Protein Concentrations

IgG concentration 1 mg/ml

Fusion Partners

Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSI myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Prior to reconstitution store at +4^oC.
After reconstitution store at +4^oC.
DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Shelf Life: 12 months from date of reconstitution.

More Information

Regulatory: For research purposes only

Applications of CD21 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry	Neat	1/10
Flow Cytometry	1/25	1/200
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
Flow Cytometry	Neat	1/10

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications
Mouse IgG1 Negative Control:FITC	MCA928F	100 Tests	F
Mouse IgG1 Negative Control	MCA928	100 Tests	F
Mouse IgG1 Negative Control:RPE	MCA928PE	100 Tests	F

Application Based External Images

Flow Cytometry

Immunofluorescence

Product Specific References

References for CD21 antibody

Howard, C.J. et al. (1991) Summary of workshop findings for leukocyte antigens of cattle.
Vet Immunol Immunopathol. 27 (1-3): 21-7.
Naessens, J. et al. (1990) Characterization of a bovine leucocyte differentiation antigen of 145,000 Mw restricted to B lymphocytes.
Immunology 69: 525-30.
Sopp, P. & Howard, C.J. (2001) IFN gamma and IL-4 production by CD4, CD8 and WC1 gamma delta TCR(+) T cells from cattle lymph nodes and blood.
Vet Immunol Immunopathol. 81 (1-2): 85-96.
Lwin, S. et al. (2009) Immune cell types involved in early uptake and transport of recombinant mouse prion protein in Peyer's patches of calves.
Cell Tissue Res. 338: 343-54.
Breugelmans, S. et al. (2011) Immunoassay of lymphocyte subsets in ovine palatine tonsils.
Acta Histochem. 113: 416-22.
Halliday, S. et al. (2005) Expression of PrPC on cellular components of sheep blood.
J Gen Virol. 86 (Pt 5): 1571-9.
Brackenbury, L.S. et al. (2005) Identification of a cell population that produces alpha/beta interferon in vitro and in vivo in response to noncytopathic bovine viral diarrhea virus.
J Virol. 79: 7738-44.
Breugelmans, S. et al. (2011) Differences between the ovine tonsils based on an immunohistochemical quantification of the lymphocyte subpopulations.
Comp Immunol Microbiol Infect Dis. 34: 217-25.
Richt, J.A. et al. (2007) Production of cattle lacking prion protein.
Nat Biotechnol. 25: 132-8.
Brujeni, G.N. et al. (2010) Bovine immunodeficiency virus and bovine leukemia virus and their mixed infection in Iranian Holstein cattle.
J Infect Dev Ctries. 4 (9): 576-9.
Kiku, Y. et al. (2010) Decrease in bovine CD14 positive cells in colostrum is associated with the incidence of mastitis after calving.
Vet Res Commun. 34: 197-203.
Pilla, R. et al. (2012) Long-term study of MRSA ST1, t127 mastitis in a dairy cow.
Vet Rec. 170: 312.
Chattha, K.S. et al. (2010) Immunohistochemical investigation of cells expressing CD21, membrane IgM, CD32 and a follicular dendritic cell marker in the lymphoid tissues of neonatal calves.
Vet Immunol Immunopathol. 137: 284-90.
Weiss, D.J. et al. (2006) Mucosal immune response in cattle with subclinical Johne's disease.
Vet Pathol. 43: 127-35.
Summers, C. et al. (2012) The distribution of immune cells in the lungs of classical and atypical ovine pulmonary adenocarcinoma.
Vet Immunol Immunopathol. 146: 1-7.
Sigurdson, C.J. et al. (2002) PrP(CWD) lymphoid cell targets in early and advanced chronic wasting disease of mule deer.
J Gen Virol. 83: 2617-28.
Dagleish, M.P.et al. (2012) Immunophenotype of cells within cervine rectoanal mucosa-associated lymphoid tissue and mesenteric lymph nodes.
J Comp Pathol. 146: 365-71.
Edwards, J.C. et al. (2010) PrP(Sc) is associated with B cells in the blood of scrapie-infected sheep.
Virology. 405: 110-9.
Brodzki, P. et al. (2014) Phenotyping of leukocytes and granulocyte and monocyte phagocytic activity in the peripheral blood and uterus of cows with endometritis.
Theriogenology. 82: 403-10.
Meganck, V. et al. (2014) Development of a method for isolating bovine colostrum mononuclear leukocytes for phenotyping and functional studies.
Vet J. 200: 294-8.
Booth, J.S. et al. (2010) Co-stimulation with TLR7/8 and TLR9 agonists induce down-regulation of innate immune responses in sheep blood mononuclear and B cells.
Dev Comp Immunol. 34 (5): 572-8.
Nikbakht Brujeni, G. et al. (2016) Association of BoLA-DRB3.2 Alleles with BLV Infection Profiles (Persistent Lymphocytosis/Lymphosarcoma) and Lymphocyte Subsets in Iranian Holstein Cattle.
Biochem Genet. 54 (2): 194-207.
Kruger, E.F. et al. (2003) Bovine monocytes induce immunoglobulin production in peripheral blood B lymphocytes.
Dev Comp Immunol. 27 (10): 889-97.
De Matteis G et al. (2016) Evaluation of leptin receptor expression on buffalo leukocytes.
Vet Immunol Immunopathol. 177: 16-23.
Ekman, A. et al. (2010) B-cell development in bovine fetuses proceeds via a pre-B like cell in bone marrow and lymph nodes.
Dev Comp Immunol. 34 (8): 896-903.
Silva, A.P. et al. (2015) Encapsulated Brucella ovis Lacking a Putative ATP-Binding Cassette Transporter (ΔabcBA) Protects against Wild Type Brucella ovis in Rams.
PLoS One. 10 (8): e0136865.

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