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CD21 antibody | CC21

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Anti Bovine CD21 Antibody, clone CC21 thumbnail image 1 Anti Bovine CD21 Antibody, clone CC21 thumbnail image 2 Anti Bovine CD21 Antibody, clone CC21 thumbnail image 3
Anti Bovine CD21 Antibody, clone CC21 gallery image 1
Figure A. RPE conjugated Mouse anti Bovine CD8b (MCA1654PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated Mouse anti Bovine CD8b (MCA1654PE) and FITC conjugated Mouse anti Bovine CD21 (MCA1424F). All experiments performed on red cell lysed bovine blood gated on lymphocytes in the presence of 10% bovine serum. Data acquired on the ZE5™ Cell Analyzer.
Anti Bovine CD21 Antibody, clone CC21 gallery image 2
Figure A. RPE conjugated Mouse anti Bovine CD8 (MCA837PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated Mouse anti Bovine CD8 (MCA837PE) and FITC conjugated Mouse anti Bovine CD21 (MCA1424F). All experiments performed on red cell lysed bovine blood gated on lymphocytes in the presence of 10% bovine serum. Data acquired on the ZE5™ Cell Analyzer.
Anti Bovine CD21 Antibody, clone CC21 gallery image 3
Published customer image:
Mouse anti Bovine CD21 monoclonal antibody, clone CC21 (MCA1424GA) used to identify ovine CD21 expressing cells in heart tissue by immunofluorescence on cryostat sections.
Image caption:
Immunofluorescence examination of explanted heart valves. CD45‐positive cells could be found within (A) allogeneic and (B) xenogeneic explants. C, Allogeneic explants exhibited the lowest number of CD45‐positive cells, whereas xenogeneic (xeno) SDS/SD‐treated explants exhibited the highest number, about 40% of all invading cells. High numbers of CD11b‐positive cells were present in all (D) allogeneic and (E) xenogeneic explants; however, (F) no significant differences were found. G, In allogeneic explants, CD3‐positive T cells were almost absent, but in (H) xenogeneic explants, a low number of CD3‐positive cells could be found. I, Enzyme‐treated valves tended to contain less CD3‐positive cells as untreated explants. J, In allogeneic as well (K) xenogeneic explants, (L) only few CD21‐positive B‐cells could be found. C, F, I, L, Every dot represents one individual animal included in the analysis. For each PHV specimen, at least two sections obtained from different areas have been analyzed, counting 11 HPFs per section. All scale bars represent 100 μm.

From: Ramm R, Goecke T, Theodoridis K, Hoeffler K, Sarikouch S, Findeisen K, Ciubotaru A, Cebotari S, Tudorache I, Haverich A, Hilfiker A.
Decellularization combined with enzymatic removal of N-linked glycans and residual DNA reduces inflammatory response and improves performance of porcine xenogeneic pulmonary heart valves in an ovine in vivo model.
Xenotransplantation. 2020 Mar;27(2):e12571.
doi: 10.1111/xen.12571.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Mouse anti Bovine CD21:Alexa Fluor® 647

Mouse anti Bovine CD21:FITC

Mouse anti Bovine CD21

Mouse anti Bovine CD21:RPE

Product Type
Monoclonal Antibody
Clone
CC21
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA1424A647
Datasheet
F
SDS
100 Tests/1ml loader

MCA1424F
Datasheet
F
SDS
0.1 mg loader

MCA1424GA
Datasheet
C F IF IP
SDS
0.1 mg loader

MCA1424PE
Datasheet
F
SDS
100 Tests loader

Mouse anti Bovine CD21 monoclonal antibody, clone CC21 recognizes the bovine CD21 cell surface antigen, a ~145 kDa single pass type I membrane glycoprotein containing multiple sushi domains. CD21 is also known as complement receptor type 2. In cattle CD21 expression is restricted to B lymphocytes (Naessens et al. 1990). CD21 may be expressed on B cells as either a long or a short form (Pringle et al. 2012)

Mouse anti bovine CD21, clone CC21 has been used to demonstrate the co-expression of CD21 with PrPc on B cells of scrapie infected sheep (Halliday et al. 2005).

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Goat
Sheep
Red deer
Mule deer
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor 647 - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1ml distilled water.
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
5% Sucrose
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSI myeloma cell line.

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD21 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry Neat 1/10
Flow Cytometry 1/25 1/200
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
Flow Cytometry Neat 1/10
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 647 MCA928A647 F 100 Tests/1ml loader
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
Mouse IgG1 Negative Control MCA928 F 100 Tests loader
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests loader

Application Based External Images

Flow Cytometry

Immunofluorescence

Product Specific References

References for CD21 antibody

  1. Howard, C.J. et al. (1991) Summary of workshop findings for leukocyte antigens of cattle.
    Vet Immunol Immunopathol. 27 (1-3): 21-7.
  2. Naessens, J. et al. (1990) Characterization of a bovine leucocyte differentiation antigen of 145,000 Mw restricted to B lymphocytes.
    Immunology 69: 525-30.
  3. Sopp, P. & Howard, C.J. (2001) IFN gamma and IL-4 production by CD4, CD8 and WC1 gamma delta TCR(+) T cells from cattle lymph nodes and blood.
    Vet Immunol Immunopathol. 81 (1-2): 85-96.
  4. Lwin, S. et al. (2009) Immune cell types involved in early uptake and transport of recombinant mouse prion protein in Peyer's patches of calves.
    Cell Tissue Res. 338: 343-54.
  5. Breugelmans, S. et al. (2011) Immunoassay of lymphocyte subsets in ovine palatine tonsils.
    Acta Histochem. 113: 416-22.
  6. Halliday, S. et al. (2005) Expression of PrPC on cellular components of sheep blood.
    J Gen Virol. 86 (Pt 5): 1571-9.
  7. Brackenbury, L.S. et al. (2005) Identification of a cell population that produces alpha/beta interferon in vitro and in vivo in response to noncytopathic bovine viral diarrhea virus.
    J Virol. 79: 7738-44.
  8. Breugelmans, S. et al. (2011) Differences between the ovine tonsils based on an immunohistochemical quantification of the lymphocyte subpopulations.
    Comp Immunol Microbiol Infect Dis. 34: 217-25.
  9. Richt, J.A. et al. (2007) Production of cattle lacking prion protein.
    Nat Biotechnol. 25: 132-8.
  10. Brujeni, G.N. et al. (2010) Bovine immunodeficiency virus and bovine leukemia virus and their mixed infection in Iranian Holstein cattle.
    J Infect Dev Ctries. 4 (9): 576-9.
  11. Kiku, Y. et al. (2010) Decrease in bovine CD14 positive cells in colostrum is associated with the incidence of mastitis after calving.
    Vet Res Commun. 34: 197-203.
  12. Pilla, R. et al. (2012) Long-term study of MRSA ST1, t127 mastitis in a dairy cow.
    Vet Rec. 170: 312.
  13. Chattha, K.S. et al. (2010) Immunohistochemical investigation of cells expressing CD21, membrane IgM, CD32 and a follicular dendritic cell marker in the lymphoid tissues of neonatal calves.
    Vet Immunol Immunopathol. 137: 284-90.
  14. Weiss, D.J. et al. (2006) Mucosal immune response in cattle with subclinical Johne's disease.
    Vet Pathol. 43: 127-35.
  15. Summers, C. et al. (2012) The distribution of immune cells in the lungs of classical and atypical ovine pulmonary adenocarcinoma.
    Vet Immunol Immunopathol. 146: 1-7.
  16. Sigurdson, C.J. et al. (2002) PrP(CWD) lymphoid cell targets in early and advanced chronic wasting disease of mule deer.
    J Gen Virol. 83: 2617-28.
  17. Dagleish, M.P.et al. (2012) Immunophenotype of cells within cervine rectoanal mucosa-associated lymphoid tissue and mesenteric lymph nodes.
    J Comp Pathol. 146: 365-71.
  18. Edwards, J.C. et al. (2010) PrP(Sc) is associated with B cells in the blood of scrapie-infected sheep.
    Virology. 405: 110-9.
  19. Brodzki, P. et al. (2014) Phenotyping of leukocytes and granulocyte and monocyte phagocytic activity in the peripheral blood and uterus of cows with endometritis.
    Theriogenology. 82: 403-10.
  20. Meganck, V. et al. (2014) Development of a method for isolating bovine colostrum mononuclear leukocytes for phenotyping and functional studies.
    Vet J. 200: 294-8.
  21. Booth, J.S. et al. (2010) Co-stimulation with TLR7/8 and TLR9 agonists induce down-regulation of innate immune responses in sheep blood mononuclear and B cells.
    Dev Comp Immunol. 34 (5): 572-8.
  22. Nikbakht Brujeni, G. et al. (2016) Association of BoLA-DRB3.2 Alleles with BLV Infection Profiles (Persistent Lymphocytosis/Lymphosarcoma) and Lymphocyte Subsets in Iranian Holstein Cattle.
    Biochem Genet. 54 (2): 194-207.
  23. Kruger, E.F. et al. (2003) Bovine monocytes induce immunoglobulin production in peripheral blood B lymphocytes.
    Dev Comp Immunol. 27 (10): 889-97.
  24. De Matteis G et al. (2016) Evaluation of leptin receptor expression on buffalo leukocytes.
    Vet Immunol Immunopathol. 177: 16-23.
  25. Ekman, A. et al. (2010) B-cell development in bovine fetuses proceeds via a pre-B like cell in bone marrow and lymph nodes.
    Dev Comp Immunol. 34 (8): 896-903.
  26. Silva, A.P. et al. (2015) Encapsulated Brucella ovis Lacking a Putative ATP-Binding Cassette Transporter (ΔabcBA) Protects against Wild Type Brucella ovis in Rams.
    PLoS One. 10 (8): e0136865.
  27. Jimbo, S. et al. (2019) Natural and inducible regulatory B cells are widely distributed in ovine lymphoid tissues.
    Vet Immunol Immunopathol. 211: 44-8.
  28. Brodzki, P. et al. (2019) Selected leukocyte subpopulations in peripheral blood and uterine washings in cows before and after intrauterine administration of cefapirin and methisoprinol.
    Anim Sci J. Nov 06 [Epub ahead of print].
  29. Souza, F.N. et al. (2020) Lymphocyte proliferative responses in dairy cows supplemented with an immunomodulatory feed additive and administered polyvalent vaccination.
    Arquivo Brasileiro de Medicina Veterinária e Zootecnia. 72 (6): 2397-401.
  30. Gondaira, S. et al. (2020) Immunosuppression in Cows following Intramammary Infusion of Mycoplasma bovis.
    Infect Immun. 88 (3) Feb 20 [Epub ahead of print].
  31. Khosa, S. et al. (2020) Bovine Adenovirus-3 Tropism for Bovine Leukocyte Sub-Populations.
    Viruses. 12 (12) Dec 12 [Epub ahead of print].
  32. Ramm, R. et al. (2020) Decellularization combined with enzymatic removal of N-linked glycans and residual DNA reduces inflammatory response and improves performance of porcine xenogeneic pulmonary heart valves in an ovine in vivo model.
    Xenotransplantation. 27 (2): e12571.
  33. Ramos, A. et al. (2018) Melatonin enhances responsiveness to Dichelobacter nodosus vaccine in sheep and increases peripheral blood CD4 T lymphocytes and IgG-expressing B lymphocytes.
    Vet Immunol Immunopathol. 206: 1-8.

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