CD2 antibody | CC42

Product Code Applications Pack Size List Price Your Price Qty
MCA833GA
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SDS Safety Datasheet SDS
C F IF IP P * 0.1 mg loader
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loader
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Mouse anti Bovine CD2, clone CC42, recognizes the bovine homologue of human CD2.

Clone CC42 inhibits rosetting with SRBC and stains cells in primary and secondary lymphoid organs in patterns consistent with those seen by human CD2 monoclonal antibodies.

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Goat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from an immunised mouse were fused with cells of the mouse NS1 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/20
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin 1
Immunoprecipitation
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.

    EDTA pH8.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

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References for CD2 antibody

  1. Davis, W.C. & Splitter, G.S. (1991) Individual antigens of cattle. Bovine CD2 (BoCD2).
    Vet Immunol Immunopathol. 27: 43-50.
  2. Sopp, P. et al. (1991) Investigating monoclonal antibodies to bovine "null" cell antigens using two-colour immunofluorescence.
    Vet Immunol Immunopathol. 27 (1-3): 163-8.
  3. Eskra, L. et al. (1991) Effect of monoclonal antibodies on in vitro function of T-cell subsets.
    Vet Immunol Immunopathol. 27 (1-3): 215-22.
  4. Gutierrez, M. et al. (1999) The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques.
    Vet Immunol Immunopathol. 71 (3-4): 321-34.
  5. Fikri, Y. et al. (2000) Purification and characterisation of bovine WC1+ gammadelta T lymphocytes from peripheral blood.
    Vet Res. 31: 229-39.
  6. Brackenbury, L.S. et al. (2005) Identification of a cell population that produces alpha/beta interferon in vitro and in vivo in response to noncytopathic bovine viral diarrhea virus.
    J Virol. 79: 7738-44.
  7. Wilson, E. et al. (1999) A circulating bovine gamma delta T cell subset, which is found in large numbers in the spleen, accumulates inefficiently in an artificial site of inflammation: correlation with lack of expression of E-selectin ligands and L-selectin.
    J Immunol.162: 4914-9.
  8. Weiss, D.J. et al. (2006) Mucosal immune response in cattle with subclinical Johne's disease.
    Vet Pathol. 43: 127-35.
  9. View The Latest Product References
  10. Grell, S.N. et al. (2005) Age-dependent differences in cytokine and antibody responses after experimental RSV infection in a bovine model.
    Vaccine 23: 3412-23.
  11. Faldyna, M. et al. (1999) Leukocyte counts and lymphocyte subpopulations in the peripheral blood of pygmy goats from herd infected with Mycobacterium avium subspecies paratuberculosis.
    Vet. Med. Czech 44: 259-62
  12. Faldyna, M. et al. (2006) γδ-TCR+ CD2– lymphocytes are recruited into bovine mammary gland after stimulation.
    Veterinarni Medicina, 51: 258–64.
  13. Slama, P. et al. (2009) Effect of Staphylococcus aureus and Streptococcus uberis on apoptosis of bovine mammary gland lymphocytes.
    Res Vet Sci. 87: 233-8.
  14. Bednarek, D. et al. (2003) Effect of steroidal and non-steroidal anti-inflammatory drugs in combination with long-acting oxytetracycline on non-specific immunity of calves suffering from enzootic bronchopneumonia.
    Vet Microbiol. 96: 53-67.
  15. Hamilton, C.A. et al. (2017) Frequency and phenotype of natural killer cells and natural killer cell subsets in bovine lymphoid compartments and blood.
    Immunology. 151 (1): 89-97.
  16. Elsayed, M.S.A.E. et al. (2022) Real-time PCR using atpE, conventional PCR targeting different regions of difference, and flow cytometry for confirmation of Mycobacterium bovis. in buffaloes and cattle from the Delta area of Egypt.
    BMC Microbiol. 22 (1): 154.

Immunofluorescence

Synonyms
LFA-2
UniProt
Q148M9
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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