CD2 antibody | CC42

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Mouse anti Bovine CD2:FITC

Mouse anti Bovine CD2

Product Type
Monoclonal Antibody
Clone
CC42
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA833F F 100 Tests
MCA833GA C F IF IP P* 0.1 mg
Mouse anti Bovine CD2, clone CC42, recognizes the bovine homologue of human CD2. Clone CC42 inhibits rosetting with SRBC and stains cells in primary and secondary lymphoid organs in patterns consistent with those seen by human CD2 monoclonal antibodies.

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Goat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from an immunised mouse were fused with cells of the mouse NS1 myeloma cell line

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of CD2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry 1/10 1/20
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin 1
Immunoprecipitation
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.

    EDTA pH8.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F 100 Tests F
Mouse IgG1 Negative Control MCA928 100 Tests F

Application Based External Images

Immunofluorescence

Product Specific References

References for CD2 antibody

  1. Davis, W.C. & Splitter, G.S. (1991) Individual antigens of cattle. Bovine CD2 (BoCD2).
    Vet Immunol Immunopathol. 27: 43-50.
  2. Sopp, P. et al. (1991) Investigating monoclonal antibodies to bovine "null" cell antigens using two-colour immunofluorescence.
    Vet Immunol Immunopathol. 27 (1-3): 163-8.
  3. Eskra, L. et al. (1991) Effect of monoclonal antibodies on in vitro function of T-cell subsets.
    Vet Immunol Immunopathol. 27 (1-3): 215-22.
  4. Gutierrez, M. et al. (1999) The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques.
    Vet Immunol Immunopathol. 71 (3-4): 321-34.
  5. Fikri, Y. et al. (2000) Purification and characterisation of bovine WC1+ gammadelta T lymphocytes from peripheral blood.
    Vet Res. 31: 229-39.
  6. Brackenbury, L.S. et al. (2005) Identification of a cell population that produces alpha/beta interferon in vitro and in vivo in response to noncytopathic bovine viral diarrhea virus.
    J Virol. 79: 7738-44.
  7. Wilson, E. et al. (1999) A circulating bovine gamma delta T cell subset, which is found in large numbers in the spleen, accumulates inefficiently in an artificial site of inflammation: correlation with lack of expression of E-selectin ligands and L-selectin.
    J Immunol.162: 4914-9.
  8. Weiss, D.J. et al. (2006) Mucosal immune response in cattle with subclinical Johne's disease.
    Vet Pathol. 43: 127-35.
  9. Grell, S.N. et al. (2005) Age-dependent differences in cytokine and antibody responses after experimental RSV infection in a bovine model.
    Vaccine 23: 3412-23.
  10. Faldyna, M. et al. (1999) Leukocyte counts and lymphocyte subpopulations in the peripheral blood of pygmy goats from herd infected with Mycobacterium avium subspecies paratuberculosis.
    Vet. Med. Czech 44: 259-62
  11. Faldyna, M. et al. (2006) γδ-TCR+ CD2– lymphocytes are recruited into bovine mammary gland after stimulation.
    Veterinarni Medicina, 51: 258–264.
  12. Slama, P. et al. (2009) Effect of Staphylococcus aureus and Streptococcus uberis on apoptosis of bovine mammary gland lymphocytes.
    Res Vet Sci. 87: 233-8.
  13. Bednarek, D. et al. (2003) Effect of steroidal and non-steroidal anti-inflammatory drugs in combination with long-acting oxytetracycline on non-specific immunity of calves suffering from enzootic bronchopneumonia.
    Vet Microbiol. 96: 53-67.