CD14 antibody | CC-G33

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Anti Bovine CD14 Antibody, clone CC-G33 thumbnail image 1

Anti Bovine CD14 Antibody, clone CC-G33 thumbnail image 2

Anti Bovine CD14 Antibody, clone CC-G33 thumbnail image 3

Anti Bovine CD14 Antibody, clone CC-G33 gallery image 1 — Staining of bovine peripheral blood monocytes with Mouse anti Bovine CD14 (**MCA2678GA**)

Anti Bovine CD14 Antibody, clone CC-G33 gallery image 2 — **Published customer image:**
Mouse anti Bovine CD4 antibody, clone CC-G33 (**MCA2678GA**) used for the identification of bovine monocytes by immunofluorescence.
**Image caption:**
Representative immunofluorescence (fluorescein isothiocyanate) showing one CD14 cell (monocyte). cellular nuclei were stained with DAPI. The samples were viewed and photographed with a microscope equipped with epifluorescence illumination at a magnification of ×1000.

From: Miarelli, M. , Signorelli, F. and Matteis, G. (2013)
Tyrosine phosphorylation of monocyte-derived macrophage proteins in buffalo (*Bubalus bubalis*): A potential phenotype of natural resistance.
Open Journal of Animal Sciences, 3, 127-31.

Anti Bovine CD14 Antibody, clone CC-G33 gallery image 3 — Figure A. FITC conjugated mouse anti bovine CD14 (**MCA2678F**) and RPE conjugated mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated mouse anti bovine CD14 (**MCA2678F**) and RPE conjugated mouse anti bovine CD80 (MCA2436PE). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

Mouse anti Bovine CD14:FITC

Mouse anti Bovine CD14

Product Type: Monoclonal Antibody
Clone: CC-G33
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA2678F	F			0.1 mg
MCA2678GA	F IF			0.1 mg

Mouse anti Bovine CD14, clone CC-G33 is a monoclonal antibody recognizing bovine CD14, a GPI-anchored membrane glycoprotein and monocyte/macrophage differentiation antigen, belonging to the lipopolysaccharide receptor family, also expressed weakly on microglia and Langerhans cells.

CD14 acts as a receptor for the potent bacterial endotoxin, lipopolysaccharide (LPS), facilitated by LPS-binding protein (LBP). The binding of LPS to CD14 results in cell activation and the release of cytokines and the inflammatory response, and has been shown to upregulate the cell surface expression of adhesion molecules.

Mouse anti Bovine CD14 clone CC-G33 cross-reacts with human CD14 expressed on transfected COS-7 cells, and also recognises an epitope on ovine CD14, see Sopp et al. 1996 for details. CC-G33 has also been shown to be reactive with CD14 from the Water buffalo (Bubalus bubalis), see Mirielli et al. 2013.

Product Details

Target Species

Bovine

Species Cross-Reactivity

Target Species	Cross Reactivity
Sheep
Human
Water Buffalo

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%	Sodium Azide (NaN₃)
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Carrier Free

Yes

Immunogen

Partially purified polypeptides isolated from bovine leucocyte cell surface membrane.

Approx. Protein Concentrations

IgG concentration 0.1mg/ml

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

Fusion Partners

Spleen cells from immunised Balb/c mice were fused with cells of the NS1 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: Q95122 Related reagents
Entrez Gene: CD14 Related reagents
GO Terms: GO:0005886 plasma membrane; GO:0006954 inflammatory response; GO:0031225 anchored to membrane; GO:0045087 innate immune response
Regulatory: For research purposes only

Applications of CD14 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry
Flow Cytometry	1/10	1/200
Immunofluorescence

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse IgG1 Negative Control:FITC	MCA928F	100 Tests	F
Mouse IgG1 Negative Control	MCA928	100 Tests	F

Application Based External Images

Flow Cytometry

Immunofluorescence

Product Specific References

Source Reference

Sopp, P. et al. (1996) Identification of bovine CD14.
Vet Immunol Immunopathol. 52 (4): 323-8.

References for CD14 antibody

Villarreal-Ramos, B. et al. (2006) Influence of the nature of the antigen on the boosting of responses to mycobacteria in M. bovis-BCG vaccinated cattle.
Vaccine. 24 (47-48): 6850-8.
Pirson, C. et al. (2012) Differential effects of Mycobacterium bovis - derived polar and apolar lipid fractions on bovine innate immune cells.
Vet Res. 43: 54.
Berthon, P. & Hopkins, J. (1996) Ruminant cluster CD14.
Vet Immunol Immunopathol. 52 (4): 245-8.
Glew, E.J. et al. (2003) Differential effects of bovine viral diarrhoea virus on monocytes and dendritic cells.
J Gen Virol. 84: 1771-80.
Harris, J. et al. (2003) Expression of caveolin by bovine lymphocytes and antigen-presenting cells.
Immunology. 105: 190-5.
Yamakawa, Y. et al. (2008) Identification and functional characterization of a bovine orthologue to DC-SIGN.
J Leukoc Biol. 83: 1396-403.
Herath, S. et al. (2006) Expression and function of Toll-like receptor 4 in the endometrial cells of the uterus.
Endocrinology. 147: 562-70.
Gliddon, D.R. et al. (2004) DEC-205 expression on migrating dendritic cells in afferent lymph.
Immunology. 11: 262-72.
Villarreal-Ramos, B. et al. (2003) Investigation of the role of CD8+ T cells in bovine tuberculosis in vivo.
Infect Immun.71: 4297-303.
Leung, S.T. et al. (2000) Uterine lymphocyte distribution and interleukin expression during early pregnancy in cows.
J Reprod Fertil. 119: 25-33.
Vrieling, M. et al. (2015) Bovine Staphylococcus aureus Secretes the Leukocidin LukMF' To Kill Migrating Neutrophils through CCR1.
MBio. 6 (3): e00335.
Gibson, A. et al. (2012) Identification of a lineage negative cell population in bovine peripheral blood with the ability to mount a strong type I interferon response
Dev Comp Immunol. 36: 332-41.
Haas, K.M. and Estes, D.M. (2001) The identification and characterization of a ligand for bovine CD5.
J Immunol. 166: 3158-66.
Miarelli, M. et al. (2013) Tyrosine phosphorylation of monocyte-derived macrophage proteins in buffalo (Bubalus bubalis): A potential phenotype of natural resistance
Open J Anim Sci. 3 (2): 127-31.
Altreuther, G. et al. (2001) Morphologic and functional changes in bovine monocytes infected in vitro with the bovine leukaemia virus.
Scand J Immunol. 54: 459-69.
Brodzki, P. et al. (2014) Phenotyping of leukocytes and granulocyte and monocyte phagocytic activity in the peripheral blood and uterus of cows with endometritis.
Theriogenology. 82 (3): 403-10.
Fikri Y et al. (2002) Costimulatory molecule requirement for bovine WC1+gammadelta T cells' proliferative response to bacterial superantigens.
Scand J Immunol. 55 (4): 373-81.
Hecker YP et al. (2014) A Neospora caninum vaccine using recombinant proteins fails to prevent foetal infection in pregnant cattle after experimental intravenous challenge.
Vet Immunol Immunopathol. 162 (3-4): 142-53.
Herry, V. et al. (2017) Local immunization impacts the response of dairy cows to Escherichia coli mastitis.
Sci Rep. 7 (1): 3441.
Pepponi, I. et al. (2017) A mycobacterial growth inhibition assay (MGIA) for bovine TB vaccine development.
Tuberculosis (Edinb). 106: 118-22.

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