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CD14 antibody | CC-G33

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Anti Bovine CD14 Antibody, clone CC-G33 thumbnail image 1 Anti Bovine CD14 Antibody, clone CC-G33 thumbnail image 2 Anti Bovine CD14 Antibody, clone CC-G33 thumbnail image 3 Anti Bovine CD14 Antibody, clone CC-G33 thumbnail image 4
Anti Bovine CD14 Antibody, clone CC-G33 gallery image 1
Staining of bovine peripheral blood monocytes with Mouse anti Bovine CD14 (MCA2678GA)
Anti Bovine CD14 Antibody, clone CC-G33 gallery image 2
Published customer image:
Mouse anti Bovine CD4 antibody, clone CC-G33 (MCA2678GA) used for the identification of bovine monocytes by immunofluorescence.
Image caption:
Representative immunofluorescence (fluorescein isothiocyanate) showing one CD14 cell (monocyte). cellular nuclei were stained with DAPI. The samples were viewed and photographed with a microscope equipped with epifluorescence illumination at a magnification of ×1000.

From: Miarelli, M. , Signorelli, F. and Matteis, G. (2013)
Tyrosine phosphorylation of monocyte-derived macrophage proteins in buffalo (Bubalus bubalis): A potential phenotype of natural resistance.
Open Journal of Animal Sciences, 3, 127-31.
Anti Bovine CD14 Antibody, clone CC-G33 gallery image 3
Figure A. FITC conjugated mouse anti bovine CD14 (MCA2678F) and RPE conjugated mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated mouse anti bovine CD14 (MCA2678F) and RPE conjugated mouse anti bovine CD80 (MCA2436PE). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Anti Bovine CD14 Antibody, clone CC-G33 gallery image 4
Published customer image:
FITC conjugated Mouse anti Bovine CD14 antibody, clone CC-G33 (MCA2678F) used for the evaluation of CD14 expression on ovine peritoneal cells by flow cytometry.
Image caption:
Percentage of cell populations in the peritoneal fluid during the early stages of infection. The specific cell populations are shown as follows: (a) TCD4; (b) TCD8; (c) WC1+γδ; (d) CD14; (e) MHCII; (f) CD83. Cell subsets of uninfected (Neg. Ctl) and infected sheep (Group 1) are shown in columns. Mann-Whitney U-test was used to compare data from Groups 1 and 3. Values represent the mean ± standard deviation (SD). Asterisks indicate different levels of significance between groups: *P ≤ 0.05, **P ≤ 0.01

From: Pérez-Caballero R, Javier Martínez-Moreno F, Zafra R, Molina-Hernández V, Pacheco IL, Teresa Ruiz-Campillo M, Escamilla A, Pérez J, Martnez-Moreno Á, Buffoni L.
Comparative dynamics of peritoneal cell immunophenotypes in sheep during the early and late stages of the infection with Fasciola hepatica by flow cytometric analysis.
Parasit Vectors. 2018 11(1):640.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Bovine CD14:FITC

Mouse anti Bovine CD14

Mouse anti Bovine CD14:RPE

Product Type
Monoclonal Antibody
Clone
CC-G33
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA2678F
Datasheet
F
SDS
0.1 mg loader

MCA2678GA
Datasheet
F IF
SDS
0.1 mg loader

MCA2678PE
Datasheet
F
SDS
100 Tests loader

Mouse anti Bovine CD14, clone CC-G33 is a monoclonal antibody recognizing bovine CD14, a GPI-anchored membrane glycoprotein and monocyte/macrophage differentiation antigen, belonging to the lipopolysaccharide receptor family, also expressed weakly on microglia and Langerhans cells.

CD14 acts as a receptor for the potent bacterial endotoxin, lipopolysaccharide (LPS), facilitated by LPS-binding protein (LBP). The binding of LPS to CD14 results in cell activation and the release of cytokines and the inflammatory response, and has been shown to upregulate the cell surface expression of adhesion molecules.

Mouse anti Bovine CD14 clone CC-G33 cross-reacts with human CD14 expressed on transfected COS-7 cells, and also recognises an epitope on ovine CD14, see Sopp et al. 1996 for details. CC-G33 has also been shown to be reactive with CD14 from the Water buffalo (Bubalus bubalis), see Mirielli et al. 2013.

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Sheep
Human
Water Buffalo
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
1%Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Carrier Free
Yes
Immunogen
Partially purified polypeptides isolated from bovine leucocyte cell surface membrane.
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the NS1 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
Q95122
Entrez Gene
CD14
GO Terms
GO:0005886 plasma membrane
GO:0006954 inflammatory response
GO:0031225 anchored to membrane
GO:0045087 innate immune response
Regulatory
For research purposes only

Applications of CD14 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Flow Cytometry 1/10 1/200
Immunofluorescence
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
Mouse IgG1 Negative Control MCA928 F 100 Tests loader
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests loader

Application Based External Images

Flow Cytometry

Immunofluorescence

Product Specific References

Source Reference

  1. Sopp, P. et al. (1996) Identification of bovine CD14.
    Vet Immunol Immunopathol. 52 (4): 323-8.

References for CD14 antibody

  1. Villarreal-Ramos, B. et al. (2006) Influence of the nature of the antigen on the boosting of responses to mycobacteria in M. bovis-BCG vaccinated cattle.
    Vaccine. 24 (47-48): 6850-8.
  2. Pirson, C. et al. (2012) Differential effects of Mycobacterium bovis - derived polar and apolar lipid fractions on bovine innate immune cells.
    Vet Res. 43: 54.
  3. Berthon, P. & Hopkins, J. (1996) Ruminant cluster CD14.
    Vet Immunol Immunopathol. 52 (4): 245-8.
  4. Glew, E.J. et al. (2003) Differential effects of bovine viral diarrhoea virus on monocytes and dendritic cells.
    J Gen Virol. 84: 1771-80.
  5. Harris, J. et al. (2003) Expression of caveolin by bovine lymphocytes and antigen-presenting cells.
    Immunology. 105: 190-5.
  6. Yamakawa, Y. et al. (2008) Identification and functional characterization of a bovine orthologue to DC-SIGN.
    J Leukoc Biol. 83: 1396-403.
  7. Herath, S. et al. (2006) Expression and function of Toll-like receptor 4 in the endometrial cells of the uterus.
    Endocrinology. 147: 562-70.
  8. Gliddon, D.R. et al. (2004) DEC-205 expression on migrating dendritic cells in afferent lymph.
    Immunology. 11: 262-72.
  9. Villarreal-Ramos, B. et al. (2003) Investigation of the role of CD8+ T cells in bovine tuberculosis in vivo.
    Infect Immun.71: 4297-303.
  10. Leung, S.T. et al. (2000) Uterine lymphocyte distribution and interleukin expression during early pregnancy in cows.
    J Reprod Fertil. 119: 25-33.
  11. Vrieling, M. et al. (2015) Bovine Staphylococcus aureus Secretes the Leukocidin LukMF' To Kill Migrating Neutrophils through CCR1.
    MBio. 6 (3): e00335.
  12. Gibson, A. et al. (2012) Identification of a lineage negative cell population in bovine peripheral blood with the ability to mount a strong type I interferon response
    Dev Comp Immunol. 36: 332-41.
  13. Haas, K.M. and Estes, D.M. (2001) The identification and characterization of a ligand for bovine CD5.
    J Immunol. 166: 3158-66.
  14. Miarelli, M. et al. (2013) Tyrosine phosphorylation of monocyte-derived macrophage proteins in buffalo (Bubalus bubalis): A potential phenotype of natural resistance
    Open J Anim Sci. 3 (2): 127-31.
  15. Altreuther, G. et al. (2001) Morphologic and functional changes in bovine monocytes infected in vitro with the bovine leukaemia virus.
    Scand J Immunol. 54: 459-69.
  16. Brodzki, P. et al. (2014) Phenotyping of leukocytes and granulocyte and monocyte phagocytic activity in the peripheral blood and uterus of cows with endometritis.
    Theriogenology. 82 (3): 403-10.
  17. Fikri Y et al. (2002) Costimulatory molecule requirement for bovine WC1+gammadelta T cells' proliferative response to bacterial superantigens.
    Scand J Immunol. 55 (4): 373-81.
  18. Hecker YP et al. (2014) A Neospora caninum vaccine using recombinant proteins fails to prevent foetal infection in pregnant cattle after experimental intravenous challenge.
    Vet Immunol Immunopathol. 162 (3-4): 142-53.
  19. Herry, V. et al. (2017) Local immunization impacts the response of dairy cows to Escherichia coli mastitis.
    Sci Rep. 7 (1): 3441.
  20. Pepponi, I. et al. (2017) A mycobacterial growth inhibition assay (MGIA) for bovine TB vaccine development.
    Tuberculosis (Edinb). 106: 118-22.
  21. Pérez-caballero, R. et al. (2018) Comparative dynamics of peritoneal cell immunophenotypes in sheep during the early and late stages of the infection with Fasciola hepatica by flow cytometric analysis.
    Parasit Vectors. 11 (1): 640.

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