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CD11b antibody | CC126

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Anti Bovine CD11b Antibody, clone CC126 thumbnail image 1 Anti Bovine CD11b Antibody, clone CC126 thumbnail image 2 Anti Bovine CD11b Antibody, clone CC126 thumbnail image 3 Anti Bovine CD11b Antibody, clone CC126 thumbnail image 4 Anti Bovine CD11b Antibody, clone CC126 thumbnail image 5
Anti Bovine CD11b Antibody, clone CC126 gallery image 1
Figure A. RPE conjugated Mouse anti Bovine CD335 (MCA2365PE) and FITC conjugated Mouse IgG2b isotype control (MCA691F). Figure B. RPE conjugated Mouse anti Bovine CD335 (MCA2365PE) and FITC conjugated Mouse anti Bovine CD11b (MCA1425F). All experiments performed on red cell lysed bovine blood gated on live cells in the presence of 10% bovine serum. Data acquired on the ZE5™ Cell Analyzer.
Anti Bovine CD11b Antibody, clone CC126 gallery image 2
Figure A. Purified mouse anti bovine CD13 (MCA2338GA) detected with goat anti mouse IgG1 RPE (STAR132PE) and FITC conjugated mouse IgG2b isotype control (MCA691F). Figure B. Purified mouse anti bovine CD13 (MCA2338GA) detected with goat anti mouse IgG1 RPE (STAR132PE) and FITC conjugated mouse anti bovine CD11b (MCA1425F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Anti Bovine CD11b Antibody, clone CC126 gallery image 3
Figure A. FITC conjugated mouse anti bovine CD11b (MCA1425F) and purified mouse IgG1 isotype control (MCA928) detected with RPE conjugated goat anti mouse IgG1 (STAR132PE). Figure B. FITC conjugated mouse anti bovine CD11b (MCA1425F) and purified CD13 (MCA2338GA) detected with RPE conjugated goat anti mouse IgG1 (STAR132PE). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Anti Bovine CD11b Antibody, clone CC126 gallery image 4
Published customer image:
Mouse anti Bovine CD11b antibody, clone CC126 (MCA1425) used as a blocking antibody preventing Emeria bovis infection of bovine neutrophils.
Image caption:
CD11b surface expression and CD11b-dependent-NET formation of bovine E. bovis sporozoite-exposed PMN. (A) Bovine PMN were exposed to CFSE-labeled sporozoites (1:1) and subjected to FACS analysis probing with an antibody directed against CD11b. Non-exposed PMN in plain medium served as negative controls. The histograms represent the shift in the mean fluorescence of the PMN population analyzed and are representative of the experiments performed. (B) NET formation in the presence of anti-CD11b antibodies. Bovine PMN were pre-treated with anti-CD11b prior to sporozoite exposure. Stimulation with zymosan served as a positive control; PMN in plain medium were used for negative controls.

From: Muñoz-Caro et al.
Eimeria bovis-triggered neutrophil extracellular trap formation is CD11b-, ERK 1/2-, p38 MAP kinase- and SOCE-dependent.
Veterinary Research 2015 46:23.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Bovine CD11b Antibody, clone CC126 gallery image 5
Published customer image:
Mouse anti Bovine CD11b antibody, clone CC126 (MCA1425) used for the detection of CD11b on ovine leukocytes by flow cytometry
Image caption:
Analysis of the phenotype of ovine NKp46+ populations. (A)Two-colour flow cytometry analysis of ovine PBMC co-stained with EC1.1 and a selection of monoclonal antibodies against CD2, CD4, CD8, CD11b, CD16, WC1, IgM and MHCII. WC1 and IgM are surface molecules expressed by subsets of γδ T-cells and B-cells respectively. animals.

From: Connelley T, Storset AK, Pemberton A, MacHugh N, Brown J, Lund H, Morrison IW.
NKp46 defines ovine cells that have characteristics corresponding to NK cells.
Vet Res. 2011 Feb 23;42:37.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Bovine CD11b:Alexa Fluor® 647

Mouse anti Bovine CD11b:FITC

Mouse anti Bovine CD11b

Mouse anti Bovine CD11b:RPE

Product Type
Monoclonal Antibody
Clone
CC126
Isotype
IgG2b
Product Code Applications Pack Size List Price Quantity
MCA1425A647
Datasheet
F
MSDS
100 Tests/1ml loader

MCA1425F
Datasheet
F
MSDS
0.1 mg loader

MCA1425GA
Datasheet
C F IP
MSDS
0.1 mg loader

MCA1425PE
Datasheet
F
MSDS
100 Tests loader

Mouse anti Bovine CD11b antibody, clone CC126 recognizes the bovine CD11b cell surface antigen.

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Goat
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor 647 - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.

Storage Information

Storage
Store at +4°C or at -20°C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD11b antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry 1/10
Flow Cytometry 1/25 1/200
Immunohistology - Frozen
Immunoprecipitation
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG2b Negative Control:Alexa Fluor® 647 MCA691A647 F 100 Tests/1ml loader
Mouse IgG2b Negative Control:FITC MCA691F F 100 Tests loader
Mouse IgG2b Negative Control MCA691 F 100 Tests loader
Mouse IgG2b Negative Control:RPE MCA691PE F 100 Tests loader

Product Specific References

References for CD11b antibody

  1. Howard, C.J. & Naessens, J. (1993) Summary of workshop findings for cattle (tables 1 and 2).
    Vet Immunol Immunopathol. 39 (1-3): 25-47.
  2. Naessens, J. et al. (1993) Cross-reactivity of workshop antibodies with cells from domestic and wild ruminants.
    Vet Immunol Immunopathol. 39 (1-3): 283-90.
  3. Akesson, C.P. et al. (2008) Phenotypic characterisation of intestinal dendritic cells in sheep.
    Dev Comp Immunol. 32: 837-49.
  4. Lecchi, C. et al. (2008) Bovine alpha-1 acid glycoprotein can reduce the chemotaxis of bovine monocytes and modulate CD18 expression.
    Vet Res. 39: 50.
  5. Connelley, T. et al. (2011) NKp46 defines ovine cells that have characteristics corresponding to NK cells.
    Vet Res. 42: 37.
  6. Kallapur, S.G .et al. (2011) Pulmonary and systemic inflammatory responses to intra-amniotic IL-1α in fetal sheep.
    Am J Physiol Lung Cell Mol Physiol. 301 (3): L285-95.
  7. Albenzio, M. & Caroprese, M. (2011) Differential leucocyte count for ewe milk with low and high somatic cell count.
    J Dairy Res. 78 (1): 43-8.
  8. Graff, J.C. and Jutila, M.A. (2007) Differential regulation of CD11b on gammadelta T cells and monocytes in response to unripe apple polyphenols.
    J Leukoc Biol. 82: 603-7.
  9. Top, S. et al. (2011) Infection of nonhost species dendritic cells in vitro with an attenuated myxoma virus induces gene expression that predicts its efficacy as a vaccine vector.
    J Virol. 85: 12982-94.
  10. Albenzio, M. et al. (2012) Immune competence of the mammary gland as affected by somatic cell and pathogenic bacteria in ewes with subclinical mastitis.
    J Dairy Sci. 95: 3877-87.
  11. Muñoz-Caro, T. et al. (2015) Eimeria bovis-triggered neutrophil extracellular trap formation is CD11b-, ERK 1/2-, p38 MAP kinase- and SOCE-dependent.
    Vet Res. 46: 23.
  12. Dedieu, L. et al. (2005) Mycoplasma mycoides ssp. mycoides biotype small colony-secreted components induce apoptotic cell death in bovine leucocytes.
    Scand J Immunol. 62 (6): 528-38.
  13. Flameng, W. et al. (2014) Coating with fibronectin and stromal cell-derived factor-1α of decellularized homografts used for right ventricular outflow tract reconstruction eliminates immune response-related degeneration.
    J Thorac Cardiovasc Surg. 147 (4): 1398-1404.e2.
  14. Edwards, J.C. et al. (2010) PrP(Sc) is associated with B cells in the blood of scrapie-infected sheep.
    Virology. 405 (1): 110-9.
  15. McClenahan, D.J. et al. (2000) Association among filamentous actin content, CD11b expression, and membrane deformability in stimulated and unstimulated bovine neutrophils.
    Am J Vet Res. 61 (4): 380-6.
  16. Foulon, E. & Foucras, G. (2008) Two populations of ovine bone marrow-derived dendritic cells can be generated with recombinant GM-CSF and separated on CD11b expression.
    J Immunol Methods. 339 (1): 1-10.
  17. Lin, Y. et al. (2013) Spatial distribution of polychlorinated naphthalenes in the atmosphere across North China based on gridded field observations.
    Environ Pollut. 180: 27-33.
  18. Lamote, I. et al. (2006) Influence of sex steroids on the viability and CD11b, CD18 and CD47 expression of blood neutrophils from dairy cows in the last month of gestation.
    Vet Res. 37 (1): 61-74.
  19. Weber, B. et al. (2016) In vitro fabrication of autologous living tissue-engineered vascular grafts based on prenatally harvested ovine amniotic fluid-derived stem cells.
    J Tissue Eng Regen Med. 10 (1): 52-70.
  20. Reber, A.J. et al. (2008) Transfer of maternal colostral leukocytes promotes development of the neonatal immune system I. Effects on monocyte lineage cells.
    Vet Immunol Immunopathol. 123 (3-4): 186-96.
  21. Ozawa, T. et al. (2012) Effect of intramammary infusion of rbGM-CSF on SCC and expression of polymorphonuclear neutrophil adhesion molecules in subclinical mastitis cows.
    Vet Res Commun. 36 (1): 21-7.
  22. Zoldan, K. et al. (2014) Increase of CD25 expression on bovine neutrophils correlates with disease severity in post-partum and early lactating dairy cows.
    Dev Comp Immunol. 47 (2): 254-63.
  23. Fach, S.J. et al. (2006) Pulmonary dendritic cells isolated from neonatal and adult ovine lung tissue.
    Vet Immunol Immunopathol. 112 (3-4): 171-82.
  24. Godoy, R.F. et al. (2014) Do progenitor cells from different tissue have the same phenotype?
    Res Vet Sci. 96 (3): 454-9.
  25. Fach, S.J. et al. (2007) Neonatal ovine pulmonary dendritic cells support bovine respiratory syncytial virus replication with enhanced interleukin (IL)-4 And IL-10 gene transcripts.
    Viral Immunol. 20 (1): 119-30.
  26. Sassa, Y. et al. (2010) Bovine macrophage degradation of scrapie and BSE PrPSc.
    Vet Immunol Immunopathol. 133 (1): 33-9.
  27. Summers, C. et al. (2005) An influx of macrophages is the predominant local immune response in ovine pulmonary adenocarcinoma.
    Vet Immunol Immunopathol. 106 (3-4): 285-94.
  28. Pilla, R. et al. (2013) Differential cell count as an alternative method to diagnose dairy cow mastitis.
    J Dairy Sci. 96 (3): 1653-60.
  29. Conejeros, I. et al. (2015) Effect of the synthetic Toll-like receptor ligands LPS, Pam3CSK4, HKLM and FSL-1 in the function of bovine polymorphonuclear neutrophils.
    Dev Comp Immunol. 52 (2): 215-25.
  30. Merriman, K.E. et al. (2018) Intramammary 25-hydroxyvitamin D3 treatment modulates innate immune responses to endotoxin-induced mastitis.
    J Dairy Sci. 101 (8): 7593-607.
  31. Brodzki, P. et al. (2019) Selected leukocyte subpopulations in peripheral blood and uterine washings in cows before and after intrauterine administration of cefapirin and methisoprinol.
    Anim Sci J. Nov 06 [Epub ahead of print].
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