CD11b antibody | CC126
Mouse anti Bovine CD11b antibody, clone CC126 (MCA1425) used as a blocking antibody preventing Emeria bovis infection of bovine neutrophils.
Image caption:
CD11b surface expression and CD11b-dependent-NET formation of bovine E. bovis sporozoite-exposed PMN. (A) Bovine PMN were exposed to CFSE-labeled sporozoites (1:1) and subjected to FACS analysis probing with an antibody directed against CD11b. Non-exposed PMN in plain medium served as negative controls. The histograms represent the shift in the mean fluorescence of the PMN population analyzed and are representative of the experiments performed. (B) NET formation in the presence of anti-CD11b antibodies. Bovine PMN were pre-treated with anti-CD11b prior to sporozoite exposure. Stimulation with zymosan served as a positive control; PMN in plain medium were used for negative controls.
From: Muñoz-Caro et al.
Eimeria bovis-triggered neutrophil extracellular trap formation is CD11b-, ERK 1/2-, p38 MAP kinase- and SOCE-dependent.
Veterinary Research 2015 46:23.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Bovine CD11b antibody, clone CC126 (MCA1425) used for the detection of CD11b on ovine leukocytes by flow cytometry
Image caption:
Analysis of the phenotype of ovine NKp46+ populations. (A)Two-colour flow cytometry analysis of ovine PBMC co-stained with EC1.1 and a selection of monoclonal antibodies against CD2, CD4, CD8, CD11b, CD16, WC1, IgM and MHCII. WC1 and IgM are surface molecules expressed by subsets of γδ T-cells and B-cells respectively. animals.
From: Connelley T, Storset AK, Pemberton A, MacHugh N, Brown J, Lund H, Morrison IW.
NKp46 defines ovine cells that have characteristics corresponding to NK cells.
Vet Res. 2011 Feb 23;42:37.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Bovine CD11b antibody, clone CC126 (MCA1425GA) used for gating bovine adipose tissue derived cells by flow cytometry.
Image caption:
Flow cytometry gating strategy used to define cell populations from the stromal vascular fraction of bovine subcutaneous adipose tissue. (a) Time parameter allowed exclusion of events bursts (1). (b) Selection of cells without debris (2) and (c) singlets (3). (d) Dead cells exclusion with Fixable Viability Dye (FVD) (4). (e) Selection of CD45+ (5) and CD45− (6) cells. (f) Selection of CD14+CD11b+(7) or CD11b−/+CD14− cells (8). (g) Gate used to analyse CD14+CD11b+MHC-II+CD45+ cells (9) (macrophages). (h) Gate used to identify CD11c+ (10) and CD11c− (11) macrophages. (i) Selection of SSC-Ahigh MHC-II− cells (12). (j) Gate used to analyse SSC-AhighCD11b−/+CD14−MHC-II−CH138A−CD45+ cells (13) (mast cells). (k) Selection of CD11b+ (14) or CD11b− (15) cells in CD45− cells. (l) Gate used to analyse CH138A+CD11b+ cells (16) (neutrophils). (m) Gate used to analyse CH138A+CD11b−MHC-II−CD45- cells (17). Pseudocolor plots are representative examples of the analysis of SVF cells isolated from SAT.
From: Oliveira BM, Pinto A, Correia A, Ferreira PG, Vilanova M, Teixeira L.
Characterization of Myeloid Cellular Populations in Mesenteric and Subcutaneous Adipose Tissue of Holstein-Friesian Cows.
Sci Rep. 2020 Feb 4;10(1):1771.
doi: 10.1038/s41598-020-58678-0.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Bovine CD11b:Alexa Fluor® 647
Mouse anti Bovine CD11b:FITC
Mouse anti Bovine CD11b
Mouse anti Bovine CD11b:RPE
- Product Type
- Monoclonal Antibody
- Clone
- CC126
- Isotype
- IgG2b
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA1425A647 | F | 100 Tests/1ml |
|
|
| MCA1425F | F | 0.1 mg |
|
|
| MCA1425GA | C F IP | 0.1 mg |
|
|
| MCA1425PE | F | 100 Tests |
|
Mouse anti Bovine CD11b antibody, clone CC126 recognizes the bovine CD11b cell surface antigen.
Product Details
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Goat Sheep - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor 647 - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose - Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD11b antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | 1/10 | ||
| Flow Cytometry | 1/25 | 1/200 | |
| Immunohistology - Frozen | |||
| Immunoprecipitation | |||
| Flow Cytometry | Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2b Negative Control:Alexa Fluor® 647 | MCA691A647 | F | 100 Tests/1ml |
|
|
| Mouse IgG2b Negative Control:FITC | MCA691F | F | 100 Tests |
|
|
| Mouse IgG2b Negative Control | MCA691 | F | 100 Tests |
|
|
| Mouse IgG2b Negative Control:RPE | MCA691PE | F | 100 Tests |
|
Product Specific References
References for CD11b antibody
-
Howard, C.J. & Naessens, J. (1993) Summary of workshop findings for cattle (tables 1 and 2).
Vet Immunol Immunopathol. 39 (1-3): 25-47. -
Naessens, J. et al. (1993) Cross-reactivity of workshop antibodies with cells from domestic and wild ruminants.
Vet Immunol Immunopathol. 39 (1-3): 283-90. -
Akesson, C.P. et al. (2008) Phenotypic characterisation of intestinal dendritic cells in sheep.
Dev Comp Immunol. 32: 837-49. -
Lecchi, C. et al. (2008) Bovine alpha-1 acid glycoprotein can reduce the chemotaxis of bovine monocytes and modulate CD18 expression.
Vet Res. 39: 50. -
Connelley, T. et al. (2011) NKp46 defines ovine cells that have characteristics corresponding to NK cells.
Vet Res. 42: 37. -
Kallapur, S.G .et al. (2011) Pulmonary and systemic inflammatory responses to intra-amniotic IL-1α in fetal sheep.
Am J Physiol Lung Cell Mol Physiol. 301 (3): L285-95. -
Albenzio, M. & Caroprese, M. (2011) Differential leucocyte count for ewe milk with low and high somatic cell count.
J Dairy Res. 78 (1): 43-8. -
Graff, J.C. and Jutila, M.A. (2007) Differential regulation of CD11b on gammadelta T cells and monocytes in response to unripe apple polyphenols.
J Leukoc Biol. 82: 603-7. -
Top, S. et al. (2011) Infection of nonhost species dendritic cells in vitro with an attenuated myxoma virus induces gene expression that predicts its efficacy as a vaccine vector.
J Virol. 85: 12982-94. -
Albenzio, M. et al. (2012) Immune competence of the mammary gland as affected by somatic cell and pathogenic bacteria in ewes with subclinical mastitis.
J Dairy Sci. 95: 3877-87. -
Muñoz-Caro, T. et al. (2015) Eimeria bovis-triggered neutrophil extracellular trap formation is CD11b-, ERK 1/2-, p38 MAP kinase- and SOCE-dependent.
Vet Res. 46: 23. -
Dedieu, L. et al. (2005) Mycoplasma mycoides ssp. mycoides biotype small colony-secreted components induce apoptotic cell death in bovine leucocytes.
Scand J Immunol. 62 (6): 528-38. -
Flameng, W. et al. (2014) Coating with fibronectin and stromal cell-derived factor-1α of decellularized homografts used for right ventricular outflow tract reconstruction eliminates immune response-related degeneration.
J Thorac Cardiovasc Surg. 147 (4): 1398-1404.e2. -
Edwards, J.C. et al. (2010) PrP(Sc) is associated with B cells in the blood of scrapie-infected sheep.
Virology. 405 (1): 110-9. -
McClenahan, D.J. et al. (2000) Association among filamentous actin content, CD11b expression, and membrane deformability in stimulated and unstimulated bovine neutrophils.
Am J Vet Res. 61 (4): 380-6. -
Foulon, E. & Foucras, G. (2008) Two populations of ovine bone marrow-derived dendritic cells can be generated with recombinant GM-CSF and separated on CD11b expression.
J Immunol Methods. 339 (1): 1-10. -
Lin, Y. et al. (2013) Spatial distribution of polychlorinated naphthalenes in the atmosphere across North China based on gridded field observations.
Environ Pollut. 180: 27-33. -
Lamote, I. et al. (2006) Influence of sex steroids on the viability and CD11b, CD18 and CD47 expression of blood neutrophils from dairy cows in the last month of gestation.
Vet Res. 37 (1): 61-74. -
Weber, B. et al. (2016) In vitro fabrication of autologous living tissue-engineered vascular grafts based on prenatally harvested ovine amniotic fluid-derived stem cells.
J Tissue Eng Regen Med. 10 (1): 52-70. -
Reber, A.J. et al. (2008) Transfer of maternal colostral leukocytes promotes development of the neonatal immune system I. Effects on monocyte lineage cells.
Vet Immunol Immunopathol. 123 (3-4): 186-96. -
Ozawa, T. et al. (2012) Effect of intramammary infusion of rbGM-CSF on SCC and expression of polymorphonuclear neutrophil adhesion molecules in subclinical mastitis cows.
Vet Res Commun. 36 (1): 21-7. -
Zoldan, K. et al. (2014) Increase of CD25 expression on bovine neutrophils correlates with disease severity in post-partum and early lactating dairy cows.
Dev Comp Immunol. 47 (2): 254-63. -
Fach, S.J. et al. (2006) Pulmonary dendritic cells isolated from neonatal and adult ovine lung tissue.
Vet Immunol Immunopathol. 112 (3-4): 171-82. -
Godoy, R.F. et al. (2014) Do progenitor cells from different tissue have the same phenotype?
Res Vet Sci. 96 (3): 454-9. -
Fach, S.J. et al. (2007) Neonatal ovine pulmonary dendritic cells support bovine respiratory syncytial virus replication with enhanced interleukin (IL)-4 And IL-10 gene transcripts.
Viral Immunol. 20 (1): 119-30. -
Sassa, Y. et al. (2010) Bovine macrophage degradation of scrapie and BSE PrPSc.
Vet Immunol Immunopathol. 133 (1): 33-9. -
Summers, C. et al. (2005) An influx of macrophages is the predominant local immune response in ovine pulmonary adenocarcinoma.
Vet Immunol Immunopathol. 106 (3-4): 285-94. -
Pilla, R. et al. (2013) Differential cell count as an alternative method to diagnose dairy cow mastitis.
J Dairy Sci. 96 (3): 1653-60. -
Conejeros, I. et al. (2015) Effect of the synthetic Toll-like receptor ligands LPS, Pam3CSK4, HKLM and FSL-1 in the function of bovine polymorphonuclear neutrophils.
Dev Comp Immunol. 52 (2): 215-25. -
Merriman, K.E. et al. (2018) Intramammary 25-hydroxyvitamin D3 treatment modulates innate immune responses to endotoxin-induced mastitis.
J Dairy Sci. 101 (8): 7593-607. -
Brodzki, P. et al. (2019) Selected leukocyte subpopulations in peripheral blood and uterine washings in cows before and after intrauterine administration of cefapirin and methisoprinol.
Anim Sci J. Nov 06 [Epub ahead of print]. -
Poindexter, M.B. et al. (2020) Feeding supplemental 25-hydroxyvitamin D3 increases serum mineral concentrations and alters mammary immunity of lactating dairy cows.
J Dairy Sci. 103 (1): 805-22. -
Ducournau, C. et al. (2020) Effective Nanoparticle-Based Nasal Vaccine Against Latent and Congenital Toxoplasmosis in Sheep.
Front Immunol. 11: 2183. -
Risalde, M.A. et al. (2020) BVDV permissiveness and lack of expression of co-stimulatory molecules on PBMCs from calves pre-infected with BVDV.
Comp Immunol Microbiol Infect Dis. 68: 101388. -
Cangiano, L.R. et al. (2019) A bioactive extract from Olea europaea. protects newly weaned beef heifers against experimentally induced chronic inflammation.
J Anim Sci. 97 (10): 4349-4361. -
Oliveira, B.M. et al. (2020) Characterization of Myeloid Cellular Populations in Mesenteric and Subcutaneous Adipose Tissue of Holstein-Friesian Cows.
Sci Rep. 10 (1): 1771.
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