CD11b antibody | CC126

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Mouse anti Bovine CD11b:FITC

Mouse anti Bovine CD11b

Product Type
Monoclonal Antibody
Clone
CC126
Isotype
IgG2b
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1425F F 0.1 mg
MCA1425GA C F IP 0.1 mg
Mouse anti Bovine CD11b antibody, clone CC126 recognizes the bovine CD11b cell surface antigen.

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Goat
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of CD11b antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10
Flow Cytometry 1/25 1/200
Immunohistology - Frozen
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2b:FITC HCA038F 0.1 mg F
Human anti Mouse IgG2b:HRP HCA038P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG2b Negative Control:FITC MCA691F 100 Tests F
Mouse IgG2b Negative Control MCA691 100 Tests F

Product Specific References

References for CD11b antibody

  1. Howard, C.J. & Naessens, J. (1993) Summary of workshop findings for cattle (tables 1 and 2).
    Vet Immunol Immunopathol. 39 (1-3): 25-47.
  2. Naessens, J. et al. (1993) Cross-reactivity of workshop antibodies with cells from domestic and wild ruminants.
    Vet Immunol Immunopathol. 39 (1-3): 283-90.
  3. Akesson, C.P. et al. (2008) Phenotypic characterisation of intestinal dendritic cells in sheep.
    Dev Comp Immunol. 32: 837-49.
  4. Lecchi, C. et al. (2008) Bovine alpha-1 acid glycoprotein can reduce the chemotaxis of bovine monocytes and modulate CD18 expression.
    Vet Res. 39: 50.
  5. Connelley, T. et al. (2011) NKp46 defines ovine cells that have characteristics corresponding to NK cells.
    Vet Res. 42: 37.
  6. Kallapur, S.G .et al. (2011) Pulmonary and systemic inflammatory responses to intra-amniotic IL-1α in fetal sheep.
    Am J Physiol Lung Cell Mol Physiol. 301 (3): L285-95.
  7. Albenzio, M. & Caroprese, M. (2011) Differential leucocyte count for ewe milk with low and high somatic cell count.
    J Dairy Res. 78 (1): 43-8.
  8. Graff, J.C. and Jutila, M.A. (2007) Differential regulation of CD11b on gammadelta T cells and monocytes in response to unripe apple polyphenols.
    J Leukoc Biol. 82: 603-7.
  9. Top, S. et al. (2011) Infection of nonhost species dendritic cells in vitro with an attenuated myxoma virus induces gene expression that predicts its efficacy as a vaccine vector.
    J Virol. 85: 12982-94.
  10. Albenzio, M. et al. (2012) Immune competence of the mammary gland as affected by somatic cell and pathogenic bacteria in ewes with subclinical mastitis.
    J Dairy Sci. 95: 3877-87.
  11. Muñoz-Caro T et al. (2015) Eimeria bovis-triggered neutrophil extracellular trap formation is CD11b-, ERK 1/2-, p38 MAP kinase- and SOCE-dependent.
    Vet Res. 46: 23.
  12. Dedieu L et al. (2005) Mycoplasma mycoides ssp. mycoides biotype small colony-secreted components induce apoptotic cell death in bovine leucocytes.
    Scand J Immunol. 62 (6): 528-38.
  13. Flameng W et al. (2014) Coating with fibronectin and stromal cell-derived factor-1α of decellularized homografts used for right ventricular outflow tract reconstruction eliminates immune response-related degeneration.
    J Thorac Cardiovasc Surg. 147 (4): 1398-1404.e2.
  14. Edwards, J.C. et al. (2010) PrP(Sc) is associated with B cells in the blood of scrapie-infected sheep.
    Virology. 405 (1): 110-9.
  15. McClenahan DJ et al. (2000) Association among filamentous actin content, CD11b expression, and membrane deformability in stimulated and unstimulated bovine neutrophils.
    Am J Vet Res. 61 (4): 380-6.
  16. Foulon E & Foucras G (2008) Two populations of ovine bone marrow-derived dendritic cells can be generated with recombinant GM-CSF and separated on CD11b expression.
    J Immunol Methods. 339 (1): 1-10.
  17. Lin Y et al. (2013) Spatial distribution of polychlorinated naphthalenes in the atmosphere across North China based on gridded field observations.
    Environ Pollut. 180: 27-33.
  18. Lamote I et al. (2006) Influence of sex steroids on the viability and CD11b, CD18 and CD47 expression of blood neutrophils from dairy cows in the last month of gestation.
    Vet Res. 37 (1): 61-74.
  19. Weber B et al. (2013) In vitro fabrication of autologous living tissue-engineered vascular grafts based on prenatally harvested ovine amniotic fluid-derived stem cells.
    J Tissue Eng Regen Med. Jul 24. [Epub ahead of print]
  20. Reber AJ et al. (2008) Transfer of maternal colostral leukocytes promotes development of the neonatal immune system I. Effects on monocyte lineage cells.
    Vet Immunol Immunopathol. 123 (3-4): 186-96.
  21. Ozawa T et al. (2012) Effect of intramammary infusion of rbGM-CSF on SCC and expression of polymorphonuclear neutrophil adhesion molecules in subclinical mastitis cows.
    Vet Res Commun. 36 (1): 21-7.
  22. Zoldan K et al. (2014) Increase of CD25 expression on bovine neutrophils correlates with disease severity in post-partum and early lactating dairy cows.
    Dev Comp Immunol. 47 (2): 254-63.
  23. Fach SJ et al. (2006) Pulmonary dendritic cells isolated from neonatal and adult ovine lung tissue.
    Vet Immunol Immunopathol. 112 (3-4): 171-82.
  24. Godoy RF et al. (2014) Do progenitor cells from different tissue have the same phenotype?
    Res Vet Sci. 96 (3): 454-9.
  25. Fach SJ et al. (2007) Neonatal ovine pulmonary dendritic cells support bovine respiratory syncytial virus replication with enhanced interleukin (IL)-4 And IL-10 gene transcripts.
    Viral Immunol. 20 (1): 119-30.
  26. Sassa Y et al. (2010) Bovine macrophage degradation of scrapie and BSE PrPSc.
    Vet Immunol Immunopathol. 133 (1): 33-9.
  27. Summers C et al. (2005) An influx of macrophages is the predominant local immune response in ovine pulmonary adenocarcinoma.
    Vet Immunol Immunopathol. 106 (3-4): 285-94.
  28. Pilla R et al. (2013) Differential cell count as an alternative method to diagnose dairy cow mastitis.
    J Dairy Sci. 96 (3): 1653-60.
  29. Conejeros, I. et al. (2015) Effect of the synthetic Toll-like receptor ligands LPS, Pam3CSK4, HKLM and FSL-1 in the function of bovine polymorphonuclear neutrophils.
    Dev Comp Immunol. 52 (2): 215-25.